Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Acta Psychiatr Scand ; 121(3): 209-15, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19694629

RESUMO

OBJECTIVE: To examine the role of the adipose-tissue-derived low-grade inflammation markers adiponectin and resistin in major depressive disorder (MDD) in a population-based sample. METHOD: Serum levels of adiponectin and resistin were measured from 70 DSM-IV MDD subjects and 70 healthy controls. Depression severity was assessed with the 29-item Hamilton Depression Rating Scale. RESULTS: The MDD group had lowered serum adiponectin levels. Regression modelling with adjustments for age, gender, overweight, several socioeconomic and lifestyle factors, coronary heart disease and metabolic syndrome showed that each 5.0 microg/ml decrease in serum adiponectin increased the likelihood of MDD by approximately 20% (P = 0.01). The resistin levels correlated with atypical (P = 0.02), but not with typical depressive symptoms (P = 0.12). CONCLUSION: Our findings suggest that the lowered adiponectin levels in MDD are depression-specific and not explained by conventional low adiponectin-related factors such as such as coronary heart disease and metabolic disorders.


Assuntos
Adiponectina/sangue , Transtorno Depressivo Maior/metabolismo , Resistina/sangue , Adulto , Doença das Coronárias , Demografia , Transtorno Depressivo Maior/fisiopatologia , Transtorno Depressivo Maior/psicologia , Feminino , Humanos , Estilo de Vida , Masculino , Pessoa de Meia-Idade , Sobrepeso , Índice de Gravidade de Doença , Fatores Socioeconômicos
2.
Osteoarthritis Cartilage ; 17(4): 448-55, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18849174

RESUMO

OBJECTIVES: The present study was designed to reveal changes in the collagen network architecture and collagen content in cartilage during growth and maturation of pigs. METHODS: Femoral groove articular cartilage specimens were collected from 4-, 11- and 21-month-old domestic pigs (n=12 in each group). The animal care conditions were kept constant throughout the study. Polarized light microscopy was used to determine the collagen fibril network birefringence, fibril orientation and parallelism. Infrared spectroscopy was used to monitor changes in the spatial collagen content in cartilage tissue. RESULTS: During growth, gradual alterations were recorded in the collagen network properties. At 4 months of age, a major part of the collagen fibrils was oriented parallel to the cartilage surface throughout the tissue. However, the fibril orientation changed considerably as skeletal maturation progressed. At 21 months of age, the fibrils of the deep zone cartilage ran predominantly at right angles to the cartilage surface. The collagen content increased and its depthwise distribution changed during growth and maturation. A significant increase of the collagen network birefringence was observed in the deep tissue at the age of 21 months. CONCLUSIONS: The present study revealed dynamic changes of the collagen network during growth and maturation of the pigs. The structure of the collagen network of young pigs gradually approached a network with the classical Benninghoff architecture. The probable explanation for the alterations is growth of the bone epiphysis with simultaneous adaptation of the cartilage to increased joint loading. The maturation of articular cartilage advances gradually with age and offers, in principle, the possibility to influence the quality of the tissue, especially by habitual joint loading. These observations in porcine cartilage may be of significance with respect to the maturation of human articular cartilage.


Assuntos
Cartilagem Articular/crescimento & desenvolvimento , Colágeno/metabolismo , Envelhecimento/metabolismo , Envelhecimento/patologia , Animais , Cartilagem Articular/anatomia & histologia , Cartilagem Articular/metabolismo , Feminino , Microscopia de Polarização/métodos , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Sus scrofa
3.
Matrix Biol ; 20(2): 137-46, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11334715

RESUMO

Lysyl hydroxylase is an enzyme involved in collagen biosynthesis, catalyzing the hydroxylation of lysyl residues as a post-translational event. Three isoforms have been characterized so far (LH1, LH2, LH3). Our recent findings indicate that LH3 possesses, not only lysyl hydroxylase activity, but also galactosylhydroxylysyl glucosyltransferase activity [Heikkinen et al., J. Biol. Chem. 275 (2000) 36158-36163]. We report here the characterization of mouse LH2 (Plod2) and LH3/glucosyltransferase (Plod3) genes. Plod2 spans approximately 50 kb of the genomic DNA, and is organized in 20 exons, one of the exons being alternatively spliced in the RNA processing. Plod3 spans approximately 10 kb of the genomic DNA, and contains 19 exons. Analysis of the 5' flanking region with many transcription start sites reveals the lack of a TATAA box in both genes. Sequence analysis indicated many retroposon-like elements within the Plod3 gene. A comparison was carried out among the LH1, LH2 and LH3 gene structures characterized so far from different species.


Assuntos
Processamento Alternativo , Glucosiltransferases/genética , Pró-Colágeno-Lisina 2-Oxoglutarato 5-Dioxigenase/genética , Animais , Sequência de Bases , Clonagem Molecular , DNA Complementar , Humanos , Camundongos , Dados de Sequência Molecular , Isoformas de Proteínas/genética , Homologia de Sequência do Ácido Nucleico , Distribuição Tecidual
4.
J Invest Dermatol ; 116(4): 602-5, 2001 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-11286629

RESUMO

Type VI Ehlers-Danlos syndrome is a disease characterized by disturbed lysine hydroxylation of collagen. The disease is caused by mutations in lysyl hydroxylase 1 gene and it affects several organs including the cardiovascular system, the joint and musculoskeletal system, and the skin. The skin of type VI Ehlers-Danlos syndrome patients is hyperelastic, scars easily, and heals slowly and poorly. We hypothesized that providing functional lysyl hydroxylase 1 gene to the fibroblasts in and around wounds in these patients would improve healing. In this study we tested the feasibility of transfer of the lysyl hydroxylase 1 gene into fibroblasts derived from rats and a type VI Ehlers-Danlos syndrome patient (in vitro) and into rat skin (in vivo). We first cloned human lysyl hydroxylase 1 cDNA into a recombinant adenoviral vector (Ad5RSV-LH). Transfection of human type VI Ehlers-Danlos syndrome fibroblasts (about 20% of normal lysyl hydroxylase 1 activity) with the vector increased lysyl hydroxylase 1 activity in these cells to near or greater levels than that of wild type, unaffected fibroblasts. The adenoviral vector successfully transfected rat fibroblasts producing both beta-galactosidase and lysyl hydroxylase 1 gene activity. We next expanded our studies to a rodent model. Intradermal injections of the vector to the abdominal skin of rats produced lysyl hydroxylase 1 mRNA and elevated lysyl hydroxylase 1 activity, in vivo. These data suggest the feasibility of gene replacement therapy to modify skin wound healing in type VI Ehlers-Danlos syndrome patients.


Assuntos
Adenoviridae/genética , Síndrome de Ehlers-Danlos/classificação , Síndrome de Ehlers-Danlos/enzimologia , Técnicas de Transferência de Genes , Pró-Colágeno-Lisina 2-Oxoglutarato 5-Dioxigenase/metabolismo , Síndrome de Ehlers-Danlos/patologia , Fibroblastos/enzimologia , Galactosidases/genética , Humanos , Hidroxilisina/metabolismo , Pró-Colágeno-Lisina 2-Oxoglutarato 5-Dioxigenase/genética , RNA Mensageiro/metabolismo , Pele/enzimologia , Pele/metabolismo , Pele/patologia , Pele/fisiopatologia
6.
Matrix Biol ; 18(3): 325-9, 1999 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10429951

RESUMO

We report on the isolation and characterization of cDNA clones for mouse lysyl hydroxylases 1, 2 and 3 (LH1, LH2, LH3). Phylogenetic analysis using nine lysyl hydroxylase sequences from five species indicates that the isoforms are derived from an ancestral gene by two duplication events, isoforms 1 and 2 being more closely related and having resulted from a more recent duplication than isoform 3. Expression of the isoforms is highly regulated in adult mouse tissues. LH1 is strongly expressed in the liver, heart, lung, skeletal muscle and kidney tissue, LH2 expression is high in the heart, lung, kidney, eye, ovary and placenta, whereas LH3 expression is high in the heart, lung, liver and testis tissue.


Assuntos
Pró-Colágeno-Lisina 2-Oxoglutarato 5-Dioxigenase/genética , Regiões 3' não Traduzidas , Sequência de Aminoácidos , Animais , DNA Complementar , Humanos , Isoenzimas/classificação , Isoenzimas/genética , Camundongos , Dados de Sequência Molecular , Filogenia , Pró-Colágeno-Lisina 2-Oxoglutarato 5-Dioxigenase/classificação , Homologia de Sequência de Aminoácidos , Distribuição Tecidual
7.
Percept Mot Skills ; 80(3 Pt 2): 1184-6, 1995 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-7478875

RESUMO

To examine the extent to which personal goals and their appraisals are associated with problems in socialization, 20 young 'social drop-outs' (15 men, 5 women) and 21 students from a vocational school (20 men, 1 woman) were interviewed about their personal goals, related views of internality, externality, and likelihood of accomplishing goals. Analysis indicated that young adults who showed problems in socialization mentioned less frequently personal goals related to future education and housing than did the control group. Second, social drop-outs held more external and less internal views and were less optimistic about accomplishing personal goals than was the control group.


Assuntos
Aspirações Psicológicas , Objetivos , Socialização , Evasão Escolar/psicologia , Adolescente , Adulto , Feminino , Humanos , Controle Interno-Externo , Masculino , Educação Vocacional
8.
Acta Chem Scand B ; 42(8): 537-45, 1988 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-2906489

RESUMO

The crystal structure, thermal behaviour, mass spectrum and protonation of 4-[1-(2,3-dimethylphenyl)ethyl]-1H-imidazole (medetomidine) hydrochloride have been investigated. The title compound crystallizes in both hydrated and anhydrous forms, and their structures have been determined by three-dimensional X-ray structure analysis. The crystals of the anhydrous form are monoclinic and those of the hydrated form (containing one hydrate water molecule) are triclinic with unit-cell dimensions: a = 23.861(9), b = 7.721(4), c = 22.037(9) A, beta = 140.20(4) degrees, Z = 8, and space group C2/c, and a = 7.841(4), b = 8.380(3), c = 12.743(6) A, alpha = 93.66(3), beta = 102.90(3), gamma = 116.85(3) degrees, Z = 2, and space group P1, respectively. Thermal decomposition of the title compound has been interpreted from the TG, DTG and DSC curves with the help of mass spectrometry. Medetomidine hydrochloride monohydrate decomposes in four stages. The first is dehydration at 45-100 degrees C, the second is evaporation of HCl and medetomidine base at 200-320 degrees C, and the third and fourth are decomposition at 340-570 degrees C. The protonation constant is 7.04 in aqueous 0.1 M NaClO4 (25 degrees C).


Assuntos
Agonistas alfa-Adrenérgicos , Imidazóis , Cristalização , Espectrometria de Massas , Medetomidina , Modelos Moleculares , Prótons , Estereoisomerismo
9.
Endocrinology ; 120(6): 2453-9, 1987 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-3569138

RESUMO

A previously unknown melatonin metabolite was isolated by chloroform extraction and reverse phase HPLC from human and rat urine after administration of synthetic melatonin and characterized by mass spectroscopy and proton magnetic resonance spectroscopy to 1-acetyl-1,2,3,3a,8,8a-hexahydro-8a-hydroxy-5-methoxypyrrolo[2,3-b ]indole, a cyclic isomer of 2-hydroxymelatonin. This isolation was based on the fact that our melatonin antibody (a-MT-K1) cross-reacted against this novel metabolite at a level of 0.1% (melatonin 100%). In our HPLC program for indoles the cyclic 2-hydroxymelatonin eluted at 25 min, separately from synthetic indoles, between 6-hydroxymelatonin (19 min) and melatonin (35 min). In [3H] melatonin studies it was found to be present (at 25 min in our HPLC), accounting for 5% of the urinary metabolites of melatonin in the rat. Since beta-glucuronidase-arylsulfatase treatment of rat urine did not liberate the cyclic 2-hydroxymelatonin this would appear to be excreted into urine as the free form.


Assuntos
Melatonina/análogos & derivados , Melatonina/metabolismo , Adulto , Animais , Cromatografia Líquida de Alta Pressão , Feminino , Humanos , Isomerismo , Espectroscopia de Ressonância Magnética , Masculino , Espectrometria de Massas , Melatonina/urina , Ratos
11.
Anal Biochem ; 142(2): 284-9, 1984 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-6549370

RESUMO

Synthetic melatonin was iodinated by treatment with potassium iodide in the presence of an oxidizing agent, Iodo-Gen. The iodination products of melatonin were extracted with chloroform and separated by HPLC. The fraction showing immunoreactivity with respect to melatonin antisera was characterized as iodomelatonin by mass spectrometry, so that the substitution of iodine had occurred at a ring carbon atom. 1H NMR spectra showed the iodine to be incorporated at the C-2 position of the indole moiety. The N-[2-(2-iodo-5-methoxy-1H-indol-3-yl)ethyl]acetamide (2-iodomelatonin) reported here is more useful than [3H]melatonin as a tracer in melatonin radioimmunoassay. This method offers also the possibility of preparing iodinated serotonin and other indoleamines for biological studies.


Assuntos
Melatonina/análogos & derivados , Sítios de Ligação , Fenômenos Químicos , Química , Cromatografia Líquida de Alta Pressão , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Melatonina/síntese química , Oxirredução
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...