RESUMO
A high level of the nucleotide sequence conservation was found for mitochondrial nad3 gene of carrot. Three silent nucleotide substitutions differentiate nad3 open reading frames from cytoplasmic male sterile and male fertile carrots. All these differences are preserved on the RNA level. Partial and silent editing also distinguished both carrots. Three of the C to U conversions were specific to the fertile line. In the two examined carrot lines editing did not affect the mode of alteration of encoded amino acids.
Assuntos
Daucus carota/genética , Mitocôndrias/genética , Proteínas Mitocondriais , Proteínas de Plantas/genética , Edição de RNA/genética , RNA/genética , Sequência de Aminoácidos , Códon/genética , Daucus carota/citologia , Regulação da Expressão Gênica de Plantas , RNA/metabolismo , RNA Mitocondrial , RNA de Plantas/genética , RNA de Plantas/metabolismo , Reprodução/genética , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Seven populations and five mutant lines of the Andean lupin and four species from the section Albus were screened for their mitochondrial and chloroplast polymorphisms. For this purpose the RFLP method with EcoRI as a restriction enzyme was used. Lupinus luteus, Lupinus albus and Phaseolus vulgaris organellar clones as well as amplified fragments were used as probes. We found that mitochondrial probes were more suitable than chloroplast probes for identification of inter- and intra-specific variations within the examined material. Most mitochondrial probes differentiate the two species investigated. A high level of mitochondrial polymorphism was observed among the populations of L. mutabilis in contrast to monomorphism among the species in the section Albus. A limited polymorphism was detected between the mutant lines of L. mutabilis. We conclude from this study that the mitochondrial RFLP analysis is a valuable tool for identification of variability among Andean lupin populations.
Assuntos
Mitocôndrias/enzimologia , Proteínas Mitocondriais , NADH Desidrogenase/genética , Animais , Eucariotos/enzimologia , Eucariotos/genética , Fungos/enzimologia , Fungos/genética , Expressão Gênica , Proteínas de Insetos/genética , Proteínas de Plantas/genética , Plantas/enzimologia , Plantas/genética , Transcrição Gênica/genéticaRESUMO
A high level of the nucleotide sequence conservation of mitochondrial nad3 and rps12 genes was found in four lupin species. The only differences concern three nucleotides in the Lupinus albus rps12 gene and three nucleotides insertion in the L. mutabilis spacer. Northern blot analysis as well as RT-PCR confirmed cotranscription of the L. luteus genes because the transcripts detected were long enough.
