RESUMO
Eight Y-chromosome specific STR (Y-STR) loci including DYS19, DYS388, DYS389I, DYS389II, DYS390, DYS391, DYS392 and DYS393 were investigated in a group of males from Central Anatolian Region of Turkey. Healthy 59 males living in this region for at least three generations were included in the study. PCR analysis was carried out with Y-STR specific primers on genomic DNA obtained from peripheral blood samples and size determination of PCR products was performed by silver staining following 6% polyacrylamide gel electrophoresis (PAGE). DYS388 was found to be the locus with lowest diversity (D) whereas DYS389II was the locus with highest diversity. The current study presented a framework of variation for the eight Y-STR loci in Central Anatolian population.
Assuntos
Cromossomos Humanos Y , Genética Populacional , Polimorfismo Genético , Sequências de Repetição em Tandem , Impressões Digitais de DNA/métodos , Frequência do Gene , Humanos , Masculino , TurquiaRESUMO
VNTR loci are useful as genetic markers in population genetics, forensics, and chimerism detection because of their high level of variability. In this study we present frequency information for two polymorphic VNTR loci, namely, D17S30 and TP53, which we examined in 100 healthy individuals from a Turkish population.
