Sum frequency generation spectroscopy of the attachment disc of a spider.

The pyriform silk of the attachment disc of a spider was studied using infrared-visible vibrational sum frequency generation (SFG) spectroscopy. The spider can attach dragline and radial lines to many kinds of substrates in nature (concrete, alloy, metal, glass, plant branches, leaves, etc.) with the attachment disc. The adhesion can bear the spider's own weight, and resist the wind on its orb web. From our SFG spectroscopy study, the NH group of arginine side chain and/or NH2 group of arginine and glutamine side chain in the amino acid sequence of the attachment silk proteins are suggested to be oriented in the disc. It was inferred from the observed doublet SFG peaks at around 3300 cm-1 that the oriented peptide contains two kinds of structures.

Optical second-harmonic images of sacran megamolecule aggregates.

We have detected a second-order nonlinear optical response from aggregates of the ampholytic megamolecular polysaccharide sacran extracted from cyanobacterial biomaterials by using optical second-harmonic-generation (SHG) microscopy. The SHG images of sacran cotton-like lump, fibers, and cast films showed SHG intensity microspots of several tens of micrometers in size. The dependence of the SHG spot intensity on an excitation light polarization angle was observed to illustrate sacran molecular orientation in these microdomains. We also observed SHG signals around a special region of the cast film edges of sacran. These results show that sacran megamolecules aggregate in several different ways.

Ionoluminescence in the helium ion microscope.

Ionoluminescence (IL) is the emission of light from a material due to excitation by an ion beam. In this work, a helium ion microscope (HIM) has been used in conjunction with a luminescence detection system to characterize IL from materials in an analogous way to how cathodoluminescence (CL) is characterized in a scanning electron microscope (SEM). A survey of the helium ion beam induced IL characteristics, including images and spectra, of a variety of materials known to exhibit CL in an SEM is presented. Direct band-gap semiconductors that luminesce strongly in the SEM are found not do so in the HIM, possibly due to defect-related nonradiative pathways created by the ion beam. Other materials do, however, exhibit IL, including a cerium-doped garnet sample, quantum dots, and rare-earth doped LaPO4 nanocrystals. These emissions are a result of transitions between f electron states or transitions across size dependent band gaps. In all these samples, IL is found to decay with exposure to the beam, fitting well to double exponential functions. In an exploration of the potential of this technique for biological tagging applications, imaging with the IL emitted by rare-earth doped LaPO4 nanocrystals, simultaneously with secondary electron imaging, is demonstrated at a range of magnifications.

Helium ion microscopy of Lepidoptera scales.

In this report, helium ion microscopy (HIM) is used to study the micro and nanostructures responsible for structural color in the wings of two species of Lepidotera from the Papilionidae family: Papilio ulysses (Blue Mountain Butterfly) and Parides sesostris (Emerald-patched Cattleheart). Electronic charging of uncoated scales from the wings of these butterflies, due to the incident ion beam, is successfully neutralized, leading to images displaying a large depth-of-field and a high level of surface detail, which would normally be obscured by traditional coating methods used for scanning electron microscopy (SEM). The images are compared with those from variable pressure SEM, demonstrating the superiority of HIM at high magnifications. In addition, the large depth-of-field capabilities of HIM are exploited through the creation of stereo pairs that allows the exploration of the third dimension. Furthermore, the extraction of quantitative height information which matches well with cross-sectional transmission electron microscopy measurements from the literature is demonstrated.

Single-mode tellurite glass holey fiber with extremely large mode area for infrared nonlinear applications.

We report the fabrication of a large mode area tellurite holey fiber from an extruded preform, with a mode area of 3000microm(2). Robust single-mode guidance at 1.55microm was confirmed by both optical measurement and numerical simulation. The propagation loss was measured as 2.9dB/m at 1.55microm. A broad and flat supercontinuum from 0.9 to 2.5microm with 6mW output was obtained with a 9cm length of this fiber.

Analytical method to find the optimal parameters for gas detectors based on correlation spectroscopy using a Fabry-Perot interferometer.

Several designs of infrared sensors use a Fabry-Perot interferometer (FPI) to modulate the incident light. In this work we analyze the particular case where the FPI fringes are matched with very well defined rovibrational absorption lines of a target molecule such as CO(2), CO, N(2)O, or CH(4). In this kind of sensor, modulation is induced by scanning the FPI cavity length over one half of the reference wavelength. Here we present an analytical method based on the Fourier transform, which simplifies the procedure to determine the sensor response. Furthermore, this method provides a simple solution to finding the optimal FPI cavity length and mirror reflectivity. It is shown that FPI mirrors with surprisingly low reflectivity (<50%) are generally the optimum choice for target gases at atmospheric pressure. Finally, experimental measurements and simulation results are presented.