[Effectiveness of oxygen-vitamin cocktails in preventing anemia, rickets, post-vaccinal complications and the risk of perinatal pathology in infants]. / Effektivnost' kislorodno-vitaminnykh kokteilei v profilaktike anemii, rakhita, postvaktsinal'nykh oslozhnenii, realizatsii riska perinatal'noi patologii u detei rannego vozrasta.

Data are presented on the composition of oxygen-vitamin cocktales used for simultaneous prevention and treatment of rickets, anemia, dystrophy, postvaccinal side effects and realization of perinatal pathology risk into a disease after birth. Mechanisms of the action of varying components of the cocktails, methods and duration of the prophylactic course, effectiveness of their use in the catamnesis have been described.

[Decreased synthetic activity as a possible cause of the death of Escherichia coli bacteria during amino acid starvation]. / Snizhenie sinteticheskoi aktivnosti kak vozmozhnaia prichina gibeli bakterii Escherichia coli vo vremia aminokislotnogo golodaniia.

The work is concerned with studying the breakdown of proteins and RNA when a polyauxotrophic Escherichia coli strain is incubated in a salt solution without amino acids, phosphorus, nitrogen and glucose at 43 degrees C as well as the ability of starving bacterial cells to recommence protein and RNA synthesis (also in the course of phage T4 infection) and to reproduce bacteriophages T4, lambda and MS2. Within the first two hours of the incubation, 12% of proteins and 40% of RNA break down to acid-soluble fragments. Then protein degradation stops while RNA decomposition goes on, but at a lower rate. Within 4-6 h of starvation, the rate of protein and RNA synthesis drops down 4-5 times and the survival rate equals 40-60% when the cells are transferred onto a complete medium. The quantitative characteristics of phages T4, lambda and MS2 reproduction fall down in prestarved cells. The authors speculate that E. coli cells die off in the course of starvation not because some unique structure is destroyed, but owing to the fact that the activity of enzymes and ribosomes gradually declines. As a result, the synthetic activity of the cell drops down abruptly and irreversibly because the enzymes are inactivated and RNA breaks down, which eventually causes cell death.

[Survival of bacteria and the fate of Escherichia coli DNA in amino acid deficiency]. / Gibel' bakterii i sud'ba DNK Escherichia coli pri aminokislotnom golodanii.

The survival rate of an E. coli polyauxotrophous strain AB1157 and the behaviour of its DNA were studied when the strain was incubated for a long time at 43 degrees C in a medium deficient in glucose, phosphates and amino acids. Under these conditions, the survival rate fell down to 10%, but no cell lysis occurred. DNA synthesis stopped within the first two hours of starvation. Neither DNA degradation, despiralization nor decrease of its molecular weight could be detected during the entire starvation. Therefore, the death of E. coli cells under these conditions was not associated with DNA damages.