RESUMO
The study of biological effect of LH and iso-LH isolated from the hypophyses of higher vertebrates on two zoons of carp-like lower vertebrates, has shown that both preparations injected in vivo induce maturation of female loaches, while in the carp, iso-LH stimulates fish maturation and LH single and/or repeated doses (2-3 injections) do not provoke female maturation. Both preparations in vitro stimulate the maturation process and the loach oocyte ovulation, but the character of this effect is dissimilar. LH exerts a high stimulating effect on the loach oocyte maturation and a low one on ovulation. Iso-LH possesses a more complicated biologic effect, depending on its concentration in the medium. Within the limits of 0.01 to 0.2 mg/ml concentrations stimulation of 50% oocyte ovulation is observed (80% ovulating normally), while within 0.5 to 4-5 mg/ml concentrations a more pronounced ability to stimulate the maturation process, but almost the same capability as in the first concentration limits to stimulate the ovulation process, is seen. A comparison of the biological effects of both preparations on the carp oocytes in vitro has shown LH to possess marked maturation-stimulating activity and almost complete absence of ovulation-stimulating action, while iso-LH to be less active as a maturation process stimulant, but to exert a high stimulating effect on ovulation process of mature oocytes.