Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 47
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Bioorg Med Chem Lett ; 28(14): 2533-2538, 2018 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-29866517

RESUMO

Picornaviruses are non-enveloped viruses that represent a large family of positive-sense single-stranded RNA viruses including a number of causative agents of many human and animal diseases such as coxsackievirus B3 (CVB3) and rhinoviruses (HRV). In this study, we performed a high-throughput screening of a compound library composed of ∼6000 small molecules in search of potential picornavirus 3C protease (3Cpro) inhibitors. As results, we identified quinone analogues that effectively inhibited both CVB3 3Cpro and HRV 3Cpro with IC50 values in low micromolar range. Together with predicted binding modes of these compounds to the active site of the viral protease, it is implied that structural features of these non-peptidic inhibitors may act as useful scaffold for further anti-picornavirus drug design and development.


Assuntos
Antivirais/farmacologia , Benzoquinonas/farmacologia , Inibidores de Proteases/farmacologia , Rhinovirus/efeitos dos fármacos , Proteínas Virais/antagonistas & inibidores , Proteases Virais 3C , Antivirais/síntese química , Antivirais/química , Benzoquinonas/síntese química , Benzoquinonas/química , Cisteína Endopeptidases/metabolismo , Relação Dose-Resposta a Droga , Testes de Sensibilidade Microbiana , Estrutura Molecular , Inibidores de Proteases/síntese química , Inibidores de Proteases/química , Rhinovirus/enzimologia , Relação Estrutura-Atividade , Proteínas Virais/metabolismo
2.
PLoS One ; 12(3): e0173121, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28282460

RESUMO

Many cancer types are serious diseases causing mortality, and new therapeutics with improved efficacy and safety are required. Immuno-(cell)-therapy is considered as one of the promising therapeutic strategies for curing intractable cancer. In this study, we tested R2016, a newly developed heterocyclic quinone derivative, for induction of immunogenic tumor cell death and as a possible novel immunochemotherapeutic. We studied the anti-cancer effects of R2016 against LLC, a lung cancer cell line and B16F10, a melanoma cell line. LLC (non-immunogenic) and B16F10 (immunogenic) cells were killed by R2016 in dose-dependent manner. R2016 reduced the viability of both LLC and B16F10 tumor cells by inducing apoptosis and necrosis, while it demonstrated no cytotoxicity against normal splenocytes. Expression of immunogenic death markers on the cell surface of R2016 treated tumor cells including calreticulin (CRT) and heat shock proteins (HSPs) was increased along with the induction of their genes. Increased CRT expression correlated with dendritic cell (DC) uptake of dying tumor cells: the proportion of CRT+CD11c+cells was increased in the R2016-treated group. The gene transcription of Calr3, Hspb1, and Tnfaip6, which are related to immunogenicity induction of dead cells, was up-regulated in the R2016 treated tumor cells. On the other hand, ANGPT1, FGF7, and URGCP gene levels were down-regulated by R2016 treatment. This data suggests that R2016 induced immunogenic tumor cell death, and suggests R2016 as an effective anti-tumor immunochemotherapeutic modality.


Assuntos
Apoptose/efeitos dos fármacos , Carbazóis/toxicidade , Compostos Heterocíclicos/toxicidade , Quinonas/toxicidade , Animais , Antígeno CD11c/metabolismo , Calreticulina/genética , Calreticulina/metabolismo , Carbazóis/síntese química , Carbazóis/química , Carbazóis/uso terapêutico , Moléculas de Adesão Celular/genética , Moléculas de Adesão Celular/metabolismo , Linhagem Celular Tumoral , Técnicas de Cocultura , Citocinas/análise , Células Dendríticas/citologia , Células Dendríticas/efeitos dos fármacos , Células Dendríticas/metabolismo , Regulação para Baixo/efeitos dos fármacos , Ensaio de Imunoadsorção Enzimática , Feminino , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/metabolismo , Compostos Heterocíclicos/síntese química , Compostos Heterocíclicos/química , Compostos Heterocíclicos/uso terapêutico , Camundongos , Camundongos Endogâmicos C57BL , Necrose , Neoplasias/tratamento farmacológico , Neoplasias/imunologia , Neoplasias/patologia , Quinonas/síntese química , Quinonas/química , Quinonas/uso terapêutico , Regulação para Cima/efeitos dos fármacos
3.
Int J Mol Sci ; 15(11): 20403-12, 2014 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-25383681

RESUMO

We report the discovery of aurora kinase inhibitor using the fragment-based virtual screening by multi-docking strategy. Among a number of fragments collected from eMololecules, we found four fragment molecules showing potent activity (>50% at 100 µM) against aurora kinase. Based on the explored fragment scaffold, we selected two compounds in our synthesized library and validated the biological activity against Aurora kinase.


Assuntos
Aurora Quinases/antagonistas & inibidores , Avaliação Pré-Clínica de Medicamentos , Simulação de Acoplamento Molecular , Inibidores de Proteínas Quinases/análise , Inibidores de Proteínas Quinases/farmacologia , Interface Usuário-Computador , Ligantes , Relação Estrutura-Atividade
4.
Chem Pharm Bull (Tokyo) ; 62(11): 1119-24, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25196129

RESUMO

2H-[1,2,3]Triazolo[4,5-g]isoquinoline-4,9-diones and 2H-[1,2,3]triazolo[4,5-g]quinoline-4,9-diones were synthesized and tested for in vitro antifungal activity against pathogenic fungi. Many of those synthesized showed potent antifungal activity. Compounds 3a, 3b, 3g, and 3h completely inhibited the growth of all fungal species tested at the MIC level of 0.8-12.5 µg/mL. The results suggest that 2H-[1,2,3]triazolo[4,5-g]isoquinoline-4,9-diones could be antifungal agents.


Assuntos
Antifúngicos/química , Antifúngicos/farmacologia , Fungos/efeitos dos fármacos , Isoquinolinas/química , Isoquinolinas/farmacologia , Triazóis/química , Triazóis/farmacologia , Antifúngicos/síntese química , Desenho de Fármacos , Humanos , Isoquinolinas/síntese química , Testes de Sensibilidade Microbiana , Micoses/tratamento farmacológico , Relação Estrutura-Atividade , Triazóis/síntese química
5.
Chem Pharm Bull (Tokyo) ; 62(7): 668-74, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24789966

RESUMO

Benzofuro[6,7-d]thiazoles, benzofuro[7,6-d]thiazoles and 6-arylaminobenzo[d]thiazole-4,7-diones were synthesized and tested for in vitro antifungal activity against Candida, Aspergillus species and Cryptococcus neoformans. Among them tested, many of synthesized compounds showed potent antifungal activity. The compounds 4d, 6e and 6h completely inhibited the growth of all Candida and Aspergillus species tested at the MIC level of 6.3 µg/mL. The results suggest that benzofuro[6,7-d]thiazoles and 6-arylaminobenzo[d]thiazole-4,7-diones would be promising antifungal agents.


Assuntos
Antifúngicos/síntese química , Benzofuranos/química , Tiazóis/química , Antifúngicos/química , Antifúngicos/farmacologia , Aspergillus/efeitos dos fármacos , Candida/efeitos dos fármacos , Cryptococcus neoformans/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Tiazóis/síntese química , Tiazóis/farmacologia
6.
Bioorg Med Chem Lett ; 23(7): 2065-8, 2013 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-23453842

RESUMO

7-Arylamino-5,8-dioxo-5,8-dihydroisoquinoline-4-carboxylates were synthesized and tested for in vitro antifungal activity against two pathogenic strains of fungi. Most of tested compounds showed good antifungal activity. The results suggest that those 5,8-dioxo-5,8-dihydroisoquinolines would be potent antifungal agents.


Assuntos
Antifúngicos/farmacologia , Aspergillus/efeitos dos fármacos , Candida/efeitos dos fármacos , Ácidos Carboxílicos/farmacologia , Cryptococcus neoformans/efeitos dos fármacos , Isoquinolinas/farmacologia , Antifúngicos/síntese química , Antifúngicos/química , Candida/classificação , Ácidos Carboxílicos/síntese química , Ácidos Carboxílicos/química , Relação Dose-Resposta a Droga , Isoquinolinas/síntese química , Isoquinolinas/química , Testes de Sensibilidade Microbiana , Estrutura Molecular , Relação Estrutura-Atividade
7.
Bioorg Med Chem Lett ; 22(18): 5777-9, 2012 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-22902652

RESUMO

1-Thia-4b-aza-cyclopenta[b]fluorene-4,10-diones were synthesized and tested for in vitro antifungal activity against two pathogenic strains of fungi. Among them tested, many compounds showed good antifungal activity. The results suggest that 1-thia-4b-aza-cyclopenta[b]fluorene-4,10-diones would be antifungal agents.


Assuntos
Antifúngicos/síntese química , Antifúngicos/farmacologia , Fluorenos/farmacologia , Fungos/efeitos dos fármacos , Antifúngicos/química , Relação Dose-Resposta a Droga , Fluorenos/síntese química , Fluorenos/química , Fungos/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Estrutura Molecular , Estereoisomerismo , Relação Estrutura-Atividade
8.
Immune Netw ; 12(2): 66-9, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22740792

RESUMO

The immunological death induction by EY-6 on the human tumor cell lines was screened. Human colon carcinoma (HCT15, HCT116), gastric carcinoma (MKN74, SNU668), and myeloma (KMS20, KMS26, KMS34) cells were died by EY-6 treatment with dose-dependent manner. CRT expression, a typical marker for the immunological death, was increased on the EY-6-treated colorectal and gastric cancer cells. Interestingly, the effects on the myeloma cell lines were complicated showing cell line dependent differential modulation. Cytokine secretion from the EY-6 treated tumor cells were dose and cell-dependent. IFN-γ and IL-12 secretion was increased in the treated cells (200% to over 1000% of non-treated control), except HCT116, SNU668 and KMS26 cells which their secretion was declined by EY-6. Data suggest the potential of EY-6 as a new type of immuno-chemotherapeutics inducing tumor-specific cell death. Further studies are planned to confirm the efficacy of EY-6 including in vivo study.

9.
Bioorg Med Chem Lett ; 22(1): 497-9, 2012 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-22115590

RESUMO

Pyrido[1,2-a]indole-1,4-diones and benzo[f]pyrido[1,2-a]indole-6,11-diones were synthesized and tested for in vitro antifungal activity against two pathogenic strains of fungi. Among them tested, many compounds showed good antifungal activity. The results suggest that pyrido[1,2-a]indole-1,4-diones and benzo[f]pyrido[1,2-a]indole-6,11-diones would be potent antifungal agents.


Assuntos
Antifúngicos/farmacologia , Carbolinas/síntese química , Química Farmacêutica/métodos , Aspergillus flavus/metabolismo , Aspergillus niger/metabolismo , Candida albicans/metabolismo , Carbolinas/farmacologia , Desenho de Fármacos , Fungos/efeitos dos fármacos , Indóis/química , Testes de Sensibilidade Microbiana , Modelos Químicos , Saccharomyces cerevisiae/metabolismo , Fatores de Tempo
10.
Bioorg Med Chem Lett ; 22(1): 500-3, 2012 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-22113112

RESUMO

6,7-Bis(arylthio)-quinazoline-5,8-dione and furo[2,3-f]quinazolin-5-ol derivatives were synthesized and tested for in vitro antifungal activity against Candida, Aspergillus species, and Cryptococcus neoformans. Among them tested, many of furo[2,3-f]quinazolin-5-ols and 6,7-bis(arylthio)-quinazoline-5,8-diones showed good antifungal activity. The compounds completely inhibited the growth of all against Candida and Aspergillus species tested at the MIC level of 12.5µg/mL. The results suggest that furo[2,3-f]quinazolin-5-ols and 6,7-bis(arylthio)-quinazoline-5,8-diones would be promising antifungal agents.


Assuntos
Antifúngicos/farmacologia , Aspergillus/metabolismo , Candida/metabolismo , Química Farmacêutica/métodos , Cryptococcus neoformans/metabolismo , Relação Dose-Resposta a Droga , Desenho de Fármacos , Fluconazol/química , Testes de Sensibilidade Microbiana , Modelos Químicos , Quinazolinas/química , Relação Estrutura-Atividade , Compostos de Sulfidrila/química
11.
Bioorg Med Chem Lett ; 21(3): 952-5, 2011 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-21215618

RESUMO

Furo[2,3-f]quinolin-5-ol derivatives were synthesized and tested for in vitro antifungal activity against Candida,Aspergillus species, and Cryptococcus neoformans. Among them tested, many furo[2,3-f]quinolin-5-ols showed good antifungal activity. The results suggest that furo[2,3-f]quinolin-5-ols would be promising antifungal agents.


Assuntos
Antifúngicos/síntese química , Hidroxiquinolinas/química , Antifúngicos/química , Antifúngicos/farmacologia , Aspergillus/efeitos dos fármacos , Candida/efeitos dos fármacos , Hidroxiquinolinas/metabolismo , Hidroxiquinolinas/farmacocinética , Testes de Sensibilidade Microbiana , Relação Estrutura-Atividade
12.
Immune Netw ; 11(6): 383-9, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22346779

RESUMO

BACKGROUND: EY-6 is one of the newly synthesized indoledione derivatives to induce tumor cell-specific cell death. In this study, we investigated the mechanism of immunological death induced by EY-6 at mouse colon cancer cell as well as at the normal immune cell represented by dendritic cell. METHODS: C57BL/6 mouse syngeneic colon cancer cell MC38 was treated with EY-6, and analyzed by MTT for viability test, flow cytometry for confirming surface expressing molecules and ELISA for detection of cytokine secretion. Normal myeloid-dendritic cell (DC) was ex vivo cultured from bone marrow hematopoietic stem cells of C57BL/6 mice with GM-CSF and IL-4 to analyze the DC uptake of dead tumor cells and to observe the effect of EY-6 on the normal DC. RESULTS: EY-6 killed the MC38 tumor cells in a dose dependent manner (25, 50 and 100 µM) with carleticulin induction. And EY-6 induced the secretion of IFN-γ but not of TNF-α from the MC38 tumor cells. EY-6 did not kill the ex-vivo cultured DCs at the dose killing tumor cells and did slightly but not significantly induced the DC maturation. The OVA-specific cross-presentation ability of DC was not induced by chemical treatment (both MHC II and MHC I-restricted antigen presentation). CONCLUSION: Data indicate that the EY-6 induced tumor cell specific and immunological cell death by modulation of tumor cell phenotype and cytokine secretion favoring induction of specific immunity eliminating tumor cells.

13.
Bioorg Med Chem Lett ; 21(1): 427-30, 2011 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-21075635

RESUMO

6-Hydroxy-1H-carbazole-1,4(9H)-diones were synthesized and tested for in vitro antifungal activity against two pathogenic strains of fungi. Among them tested, many compounds showed good antifungal activity. The results suggest that 6-hydroxy-1H-carbazole-1,4(9H)-diones would be potent antifungal agents.


Assuntos
Antifúngicos/síntese química , Carbazóis/química , Antifúngicos/química , Antifúngicos/farmacologia , Carbazóis/síntese química , Carbazóis/farmacologia , Fungos/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Relação Estrutura-Atividade
14.
Bioorg Med Chem Lett ; 20(22): 6777-80, 2010 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-20851600

RESUMO

Benzofuran-5-ol derivatives were synthesized and tested for in vitro antifungal activity against Candida, Aspergillus species, and Cryptococcus neoformans. Among them tested, many benzofuran-5-ols showed good antifungal activity. The results suggest that benzofuran-5-ols would be promising antifungal agents.


Assuntos
Antifúngicos/síntese química , Antifúngicos/farmacologia , Benzofuranos/síntese química , Benzofuranos/farmacologia , Aspergillus/efeitos dos fármacos , Candida/efeitos dos fármacos , Cryptococcus neoformans/efeitos dos fármacos , Testes de Sensibilidade Microbiana
15.
Bioorg Med Chem Lett ; 19(20): 5924-6, 2009 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-19733068

RESUMO

Benzo[d]oxazole-4,7-diones were synthesized and tested for in vitro antifungal activity against fungi. Among them tested, many compounds showed good antifungal activity. The results suggest that benzo[d]oxazole-4,7-diones would be potent antifungal agents.


Assuntos
Antifúngicos/síntese química , Oxazóis/síntese química , Antifúngicos/química , Antifúngicos/farmacologia , Fungos/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Oxazóis/química , Oxazóis/farmacologia , Relação Estrutura-Atividade
16.
Bioorg Med Chem Lett ; 19(1): 146-8, 2009 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-19010669

RESUMO

1H-Pyrrolo[3,2-g]quinoline-4,9-diones and 4,9-dioxo-4,9-dihydro-1H-benzo[f]indoles were synthesized and tested for in vitro antifungal activity against fungi. Among them tested, many compounds showed good antifungal activity. The results suggest that 1H-pyrrolo[3,2-g]quinoline-4,9-diones and 4,9-dioxo-4,9-dihydro-1H-benzo[f]indoles would be potent antifungal agents.


Assuntos
Antifúngicos/síntese química , Indóis/síntese química , Quinolinas/síntese química , Animais , Antifúngicos/farmacologia , Fungos/efeitos dos fármacos , Indóis/farmacologia , Quinolinas/farmacologia , Relação Estrutura-Atividade
17.
Bioorg Med Chem ; 16(22): 9772-9, 2008 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-18930405

RESUMO

The abnormal proliferation and migration of vascular smooth muscle cells (SMCs) play an important role in the pathology of coronary artery atherosclerosis and restenosis following angioplasty. It was reported that some heterocyclic quinone derivatives such as 6-arylamino-quinoxaline-5,8-diones and 6-arylamino-1H-benzo[d]imidazole-4,7-diones have inhibitory activity on rat aortic smooth muscle cell (RAoSMC) proliferation. To understand the structural basis for antiproliferative activity to design more potent agents, we generated pharmacophore models of representative molecules with high activity using Genetic Algorithm with Linear Assignment of Hypermolecular Alignment of Database (GALAHAD) and aligned a series of compounds to the selected pharmacophore model, then performed three-dimensional quantitative structure-activity relationship (3D-QSAR) studies using Comparative Molecular Field Analysis (CoMFA) and Comparative Molecular Similarity Indices Analysis (CoMSIA). Good cross-validated correlations were obtained with CoMFA (resulting in q(2) of 0.734 and r(2) of 0.947) and CoMSIA (resulting in q(2) of 0.736 and r(2) of 0.913). The IC(50) values of the heterocyclic quinone derivatives on RAoSMC exhibited a strong correlation with steric and hydrophobic fields of the 3D structure of the molecules, resulting in the reliable prediction of inhibitory activity of the series of compounds.


Assuntos
Músculo Liso Vascular/efeitos dos fármacos , Miócitos de Músculo Liso/efeitos dos fármacos , Quinonas/química , Quinonas/farmacologia , Animais , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Simulação por Computador , Desenho de Fármacos , Concentração Inibidora 50 , Modelos Moleculares , Estrutura Molecular , Músculo Liso Vascular/citologia , Relação Quantitativa Estrutura-Atividade , Quinonas/síntese química , Ratos
19.
Eur J Pharmacol ; 586(1-3): 74-81, 2008 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-18402936

RESUMO

Indoledione derivatives have pronounced biological effects, i.e., cytotoxic activities against cancer cell lines and antifungal and antibacterial activities. The present study was designed to investigate the effects of YSK2821, a newly synthesized indoledione derivative, on platelet-derived growth factor (PDGF-BB)-induced vascular smooth muscle cell (VSMC) proliferation, as well as the molecular mechanisms of the anti-proliferative effects of YSK2821 in VSMCs. We found that YSK2821 caused the accumulation of cells in the G1 phase of the cell cycle and inhibited [3H]-thymidine incorporation. We demonstrated that YSK2821 remarkably decreased Akt kinase phosphorylation as the mechanism by which YSK2821 suppressed cell signal transduction events in VSMC proliferation. Furthermore, in terms of the effects of YSK2821 on cell cycle-related proteins, YSK2821 enhanced the expression of the cyclin-dependent protein kinase (CDK) inhibitor p27 and down-regulated CDK2 and cyclin E expression, but did not affect CDK4 and cyclin D1 expression. YSK2821 also inhibited the phosphorylation of Rb, a key regulator in the cell cycle. These results indicate that YSK2821, a newly synthesized indoledione derivative, may inhibit VSMC proliferation via a phosphatidylinositol (PI)-3 kinase-dependent pathway, and thus shed light on a novel role for YSK2821 as a potential preventive regulator of cardiovascular disease.


Assuntos
Proteínas de Ciclo Celular/fisiologia , Ciclo Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Músculo Liso Vascular/enzimologia , Fosfatidilinositol 3-Quinases/metabolismo , Pirróis/farmacologia , Quinolonas/farmacologia , Apoptose/efeitos dos fármacos , Western Blotting , Contagem de Células , Morte Celular/efeitos dos fármacos , Humanos , Espectroscopia de Ressonância Magnética , Músculo Liso Vascular/citologia , Músculo Liso Vascular/efeitos dos fármacos , Necrose/induzido quimicamente , Fator de Crescimento Derivado de Plaquetas/farmacologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transdução de Sinais/efeitos dos fármacos , Timidina/metabolismo
20.
Biochem Pharmacol ; 75(6): 1331-40, 2008 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-18191105

RESUMO

The increased potential for growth of vascular smooth muscle cells (VSMCs) is a key abnormality in the development of atherosclerosis and postangioplasty restenosis. Platelet-derived growth factor (PDGF)-BB is a potent mitogen for VSMCs that plays an important role in the intimal accumulation of VSMCs. This study examined the effect of JM91, a newly synthesized indoledione derivative, on the proliferation of PDGF-BB-stimulated rat aortic VSMCs. The antiproliferative effect of JM91 on rat aortic VSMCs was examined by cell counting and [(3)H]thymidine incorporation assay. The pre-incubation of JM91 (0.5-3.0 microM) significantly inhibited the proliferation and DNA synthesis of 25 ng/mL PDGF-BB-stimulated rat aortic VSMCs in a concentration-dependent manner. JM91 inhibited the PDGF-BB-stimulated phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) and Akt kinase, while had no effect on PLCgamma1 and PDGF-Rbeta activation. In addition, treatment with JM91 (0.5-3.0 microM) induced cell-cycle arrest in the G(1) phase, which was associated with the down-regulation of cyclins and CDKs. These findings suggest that the inhibitory effects of JM91 against proliferation, DNA synthesis and cell cycle progression of PDGF-BB-stimulated rat aortic VSMCs are mediated by the suppression of the ERK1/2 and PI3K/Akt signaling pathways. Furthermore, JM91 may be a potential antiproliferative agent for the treatment of atherosclerosis and angioplasty restenosis.


Assuntos
Proliferação de Células/efeitos dos fármacos , Miócitos de Músculo Liso/efeitos dos fármacos , Inibidores de Proteínas Quinases/farmacologia , Quinolonas/farmacologia , Quinonas/farmacologia , Animais , Aorta/citologia , Becaplermina , Ciclo Celular/efeitos dos fármacos , Proteínas de Ciclo Celular/metabolismo , Células Cultivadas , DNA/metabolismo , Proteína Quinase 1 Ativada por Mitógeno/antagonistas & inibidores , Proteína Quinase 3 Ativada por Mitógeno/antagonistas & inibidores , Miócitos de Músculo Liso/citologia , Miócitos de Músculo Liso/metabolismo , Inibidores de Fosfoinositídeo-3 Quinase , Fosforilação/efeitos dos fármacos , Fator de Crescimento Derivado de Plaquetas/farmacologia , Proteínas Proto-Oncogênicas c-akt/antagonistas & inibidores , Proteínas Proto-Oncogênicas c-sis , Ratos , Proteína do Retinoblastoma/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...