Predicting future coastal land use/cover change and associated sea-level impact on habitat quality in the Northwestern Coastline of Guinea-Bissau.

The assessment of coastal land use/cover (LULC) change is one of the most precise techniques for detecting spatio-temporal change in the coastal system. This study, integrated Land Change Modeler, Habitat Quality Model, and Digital Shoreline Analysis System, to quantify spacio-temporal coastal LULC change and driving forces between 2000 and 2020. Combined the CA-Markov Model with Sea Level Affecting Marshes Model (SLAMM), merged local SLR data with future representative concentration pathway (RCP8.5) scenarios, and predicted future coastal LULC change and associated sea-level rise (SLR) impact on the coastal land use and habitat quality in short-, medium- and long-term. The study area had significant coastal LULC change between 2000 and 2020. The tidal flats, whose change was driven mainly by sea level, registered a total net gain of 57.93 km2. We also observed the significant loss of developed land whose change was influenced by tidal flat with a total loss of -75.58 km2. The tidal flat will experience a stunning net gain of 80.55 km2 between 2020 and 2060, making developed land the most negatively impacted land in the study area. The study led to the conclusion that the uncontrolled conversion of saltmarshes, mixed-forest, and mangroves into agriculture and infrastructures were the main factors affecting the coastal systems, including the faster coastal erosion and accretion observed during a 20-year period. The study also concluded that a low coastal elevation of -1 m and a slope of less than 2° have contributed to coastal change. Unprecedented changes will unavoidably pose a danger to coastal ecological services, socioeconomic growth, and food security. Timely efforts should be made by establishing sustainable mitigation methods to avoid the future impact.

Population genetic history of Aristeus antennatus (Crustacea: Decapoda) in the Western and Central Mediterranean Sea.

Aristeus antennatus is an ecologically and economically important deep-water species in the Mediterranean Sea. In this study we investigated the genetic variability of A. antennatus sampled from 10 sampling stations in the Western and Central Mediterranean. By comparing our new samples with available data from the Western area, we aim to identify potential genetic stocks of A. antennatus and to reconstruct its historical demography in the Mediterranean. We analyzed two regions of mitochondrial DNA in 319 individuals, namely COI and 16S. We found two main results: i) the genetic diversity values consistent with previous data within the Mediterranean and the absence of barriers to gene flow within the Mediterranean Sea; ii) a constant long-term effective population size in almost all demes but a strong signature of population expansion in the pooled sample about 50,000 years B.P./ago. We propose two explanation for our results. The first is based on the ecology of A. antennatus. We suggest the existence of a complex meta-population structured into two layers: a deeper-dwelling stock, not affected by fishing, which preserves the pattern of historical demography; and genetically homogeneous demes inhabiting the fishing grounds. The larval dispersal, adult migration and continuous movements of individuals from "virgin" deeper grounds not affected by fishing to upper fishing areas support an effective 'rescue effect' contributing to the recovery of the exploited stocks and explain their genetic homogeneity throughout the Mediterranean Sea. The second is based on the reproduction model of this shrimp: the high variance in offspring production calls for a careful interpretation of the data observed under classical population genetics and Kingman's coalescent. In both cases, management policies for A. antennatus will therefore require careful evaluation of the meta-population dynamics of all stocks in the Mediterranean. In the future, it will be particularly relevant to sample the deepest ones directly.

Diversity of zoonotic enterohepatic Helicobacter species and detection of a putative novel gastric Helicobacter species in wild and wild-born captive chimpanzees and western lowland gorillas.

A number of Helicobacter species cause gastrointestinal or hepatic disease in humans, including H. pylori, gastric non-H. pylori helicobacters from animal origin and enterohepatic Helicobacter species. Little is known on the presence of Helicobacter species in great apes, our closest living relatives and potential reservoirs of microorganisms that might emerge in humans. The aim of the present study was to investigate the presence of gastric and enterohepatic Helicobacter species in African chimpanzees and gorillas. Fresh fecal samples were collected from wild endangered chimpanzees and critically endangered western lowland gorillas from different African National Parks, as well as wild-born captive animals from primate sanctuaries. Intact Helicobacter bacteria were demonstrated in feces by fluorescence in situ hybridization. Screening using a Helicobacter genus-specific PCR revealed the presence of Helicobacter DNA in the majority of animals in all groups. Cloning and sequencing of 16S rRNA gene fragments revealed a high homology to sequences from various zoonotic enterohepatic Helicobacter species, including H. cinaedi and H. canadensis. A number of gorillas and chimpanzees also tested positive using PCR assays designed to amplify part of the ureAB gene cluster and the hsp60 gene of gastric helicobacters. Phylogenetic analysis revealed the presence of a putative novel zoonotic gastric Helicobacter taxon/species. For this species, we propose the name 'Candidatus Helicobacter homininae', pending isolation and further genetic characterization. The presence of several Helicobacter species not only implies a possible health threat for these endangered great apes, but also a possible zoonotic transmission of gastric and enterohepatic helicobacters from these primate reservoirs to humans.

Gastrointestinal symbionts of chimpanzees in Cantanhez National Park, Guinea-Bissau with respect to habitat fragmentation.

One of the major factors threatening chimpanzees (Pan troglodytes verus) in Guinea-Bissau is habitat fragmentation. Such fragmentation may cause changes in symbiont dynamics resulting in increased susceptibility to infection, changes in host specificity and virulence. We monitored gastrointestinal symbiotic fauna of three chimpanzee subpopulations living within Cantanhez National Park (CNP) in Guinea Bissau in the areas with different levels of anthropogenic fragmentation. Using standard coproscopical methods (merthiolate-iodine formalin concentration and Sheather's flotation) we examined 102 fecal samples and identified at least 13 different symbiotic genera (Troglodytella abrassarti, Troglocorys cava, Blastocystis spp., Entamoeba spp., Iodamoeba butschlii, Giardia intestinalis, Chilomastix mesnili, Bertiella sp., Probstmayria gombensis, unidentified strongylids, Strongyloides stercoralis, Strongyloides fuelleborni, and Trichuris sp.). The symbiotic fauna of the CNP chimpanzees is comparable to that reported for other wild chimpanzee populations, although CNP chimpanzees have a higher prevalence of Trichuris sp. Symbiont richness was higher in chimpanzee subpopulations living in fragmented forests compared to the community inhabiting continuous forest area. We reported significantly higher prevalence of G. intestinalis in chimpanzees from fragmented areas, which could be attributed to increased contact with humans and livestock.

Distribution of the entodiniomorphid ciliate Troglocorys cava Tokiwa, Modrý, Ito, Pomajbíková, Petrzelková, & Imai, , (Entodiniomorphida: Blepharocorythidae) in wild and captive chimpanzees.

Trophozoites of Troglocorys cava were detected in all but one of the wild chimpanzee populations from Rubondo Island (Tanzania), with a prevalence ranging between 20% and 78%. However, the ciliate was absent in all captive groups. Prevalence appeared to increase with the number of sequential samples taken from a particular individual and reached 95.5% in wild individuals sampled at least 4 times.

Mortalidade materna: Hospital Geral de Fortaleza (1975-1991) / Maternal mortality: Hospital Geral de Fortaleza (1975-1991)

Quarenta e três mortes maternas ocorreram na Clínica Obstétrica do Hospital Geral de Fortaleza Sistema Estadual de Saúde (HGF-SES), no período de janeiro de 1975 a julho de 1991. As causas obstétricas diretas contribuiram com um maior percentual (69,77 por cento), sendo eclâmpsia, hemorragia e infecçao as principais afecçoes em ordem decrescente de freqüência, seguidas pelas causas obstétricas indiretas (ll,63 por cento)e as nao-obstétricas (6,98 por cento). Comparamos dois períodos: janeiro de 1975 a dezembro de 1987, com um coeficiente de mortalidade matema (CMM) de 206, e janeiro de l988 a julho de l99l, comum CMM de lO4, everificamos uma diminuiçao significativa do mesmo, apesar de este ser ainda alto, visto que nosso serviço atende gestantes de risco. Observamos, ainda, que a maioria das mortes matemas sao evitáveis, sendo necessária a instalaçao de um melhor programa de saúde pública.