Radio-miRs: a comprehensive view of radioresistance-related microRNAs.

Radiotherapy is a key treatment option for a wide variety of human tumors, employed either alone or alongside with other therapeutic interventions. Radiotherapy uses high-energy particles to destroy tumor cells, blocking their ability to divide and proliferate. The effectiveness of radiotherapy is due to genetic and epigenetic factors that determine how tumor cells respond to ionizing radiation. These factors contribute to the establishment of resistance to radiotherapy, which increases the risk of poor clinical prognosis of patients. Although the mechanisms by which tumor cells induce radioresistance are unclear, evidence points out several contributing factors including the overexpression of DNA repair systems, increased levels of reactive oxygen species, alterations in the tumor microenvironment, and enrichment of cancer stem cell populations. In this context, dysregulation of microRNAs or miRNAs, critical regulators of gene expression, may influence how tumors respond to radiation. There is increasing evidence that miRNAs may act as sensitizers or enhancers of radioresistance, regulating key processes such as the DNA damage response and the cell death signaling pathway. Furthermore, expression and activity of miRNAs have shown informative value in overcoming radiotherapy and long-term radiotoxicity, revealing their potential as biomarkers. In this review, we will discuss the molecular mechanisms associated with the response to radiotherapy and highlight the central role of miRNAs in regulating the molecular mechanisms responsible for cellular radioresistance. We will also review radio-miRs, radiotherapy-related miRNAs, either as sensitizers or enhancers of radioresistance that hold promise as biomarkers or pharmacological targets to sensitize radioresistant cells.

Biotechnological potential of yeast cell wall: An overview.

The yeast cell wall is a complex structure whose main function is to protect the cell from physical and chemical damage, providing it with rigidity. It is composed of a matrix of covalently linked polysaccharides and proteins, including ß-glucans, mannoproteins, and chitin, whose proportion can vary according to the yeast species and environmental conditions. The main components of the yeast cell wall have relevant properties that expand the possibilities of use in different industrial sectors, such as pharmaceutical, food, medical, veterinary, and cosmetic. Some applications include bioremediation, enzyme immobilization, animal feed, wine production, and hydrogel production. In the literature it is the description of the cell wall composition of model species like Saccharomyces cerevisiae and Candida albicans, however, it is important to know that this composition can vary according to the species or the culture medium conditions. Thus, understanding the structural composition of different species holds promise as an alternative to expanding the utilization of residual yeast from different bioprocesses. In the context of a circular economy, the conversion of residual yeast into valuable products is an attractive prospect for researchers aiming to develop sustainable technologies. This review provides an overview of yeast cell wall composition and its significance in biotechnological applications, considering prospects to increase the diversification of these compounds in industry.

Fungal Melanonychia: A Systematic Review.

Fungal melanonychia is an uncommon condition, most typically caused by opportunistic melanin-producing pigmented filamentous fungi in the nail plate. In the present study, the clinical characteristics of patients diagnosed with fungal melanonychia were analyzed through a systematic review of cases reported in the literature. The MESH terms used for the search were "melanonychia" AND "fungal" OR "fungi" through four databases: PubMed, SciELO, Google scholar and SCOPUS. After discarding inadequate articles using the exclusion criteria, 33 articles with 133 cases were analyzed, of which 44% were women, 56% were men and the age range was between 9 and 87 years. The majority of cases were reported in Turkey followed by Korea and Italy. Frequent causal agents detected were Trichophyton rubrum as non-dematiaceous in 55% and Neoscytalidium dimidiatum as dematiaceous in 8%. Predisposing factors included nail trauma, migration history, employment and/or outdoor activities. Involvement in a single nail was presented in 45% of the cases, while more than one affected nail was identified in 21%, with a range of 2 to 10 nails. Regarding the clinical classification, 41% evidenced more than one type of melanonychia, 21% corresponded to the longitudinal pattern and 13% was of total diffuse type. Likewise, the usual dermoscopic pattern was multicolor pigmentation. It is concluded that fungal melanonychia is an uncommon variant of onychomycosis and the differential diagnosis is broad, which highlights the complexity of this disease.

Molecular and clinical characterization of a founder mutation causing G6PC3 deficiency.

Background: G6PC3 deficiency is a rare genetic disorder that causes syndromic congenital neutropenia. It is driven by the intracellular accumulation of a metabolite named 1,5-anhydroglucitol-6-phosphate (1,5-AG6P) that inhibits glycolysis. Patients display heterogeneous extra-hematological manifestations, contributing to delayed diagnosis. Objective: The G6PC3 c.210delC variant has been identified in patients of Mexican origin. We set out to study the origin and functional consequence of this mutation. Furthermore, we sought to characterize the clinical phenotypes caused by it. Methods: Using whole-genome sequencing data, we conducted haplotype analysis to estimate the age of this allele and traced its ancestral origin. We examined how this mutation affected G6PC3 protein expression and performed extracellular flux assays on patient-derived cells to characterize how this mutation impacts glycolysis. Finally, we compared the clinical presentations of patients with the c.210delC mutation relative to other G6PC3 deficient patients published to date. Results: Based on the length of haplotypes shared amongst ten carriers of the G6PC3 c.210delC mutation, we estimated that this variant originated in a common ancestor of indigenous American origin. The mutation causes a frameshift that introduces a premature stop codon, leading to a complete loss of G6PC3 protein expression. When treated with 1,5-anhydroglucitol (1,5-AG), the precursor to 1,5-AG6P, patient-derived cells exhibited markedly reduced engagement of glycolysis. Clinically, c.210delC carriers display all the clinical features of syndromic severe congenital neutropenia type 4 observed in prior reports of G6PC3 deficiency. Conclusion: The G6PC3 c.210delC is a loss-of-function mutation that arose from a founder effect in the indigenous Mexican population. These findings may facilitate the diagnosis of additional patients in this geographical area. Moreover, the in vitro 1,5-AG-dependent functional assay used in our study could be employed to assess the pathogenicity of additional G6PC3 variants.

The anticancer activity of bovine lactoferrin is reduced by deglycosylation and it follows a different pathway in cervix and colon cancer cells.

Bovine lactoferrin (bLF) is a glycosylated protein with purported beneficial properties. The aim of this work was to determine the role of bLF glycosylation in the adhesion, internalization, and growth inhibition of cancer cells. The viability of cervix (HeLa) and colon (Caco-2) cancer cells (MTT assay and epifluorescence microscopy) was inhibited by bLF, while deglycosylated bLF (bLFdeg) had no effect. Adhesion to cell surfaces was quantified by immunofluorescence assay and showed that bLF was able to bind more efficiently to both cell lines than bLFdeg. Microscopic observations indicated that bLF glycosylation favored bLF binding to epithelial cells and that it was endocytosed through caveolin-1-mediated internalization. In addition, the mechanism of action of bLF on cancer cell proliferation was investigated by determining the amount of phosphorylated intermediates of signaling pathways such as epidermal growth factor receptor (EGFR), extracellular signal-regulated kinase (ERK) and protein kinase B (known as Akt). Chemoluminescence immunoassay of phosphorylated intermediates showed that bLF inhibited Akt phosphorylation, consistent with its growth inhibiting activity. This assay also indicated that the bLF receptor/signaling pathways may be different in the two cell lines, Caco-2 and HeLa. This work confirmed the effect of glycosylated bLF in inhibiting cancer cell growth and that glycosylation is required for optimal surface adhesion, internalization, and inhibition of the ERK/Akt pathway of cell proliferation through glycosylated cell surface receptors.

Testing intergroup contact theory through a natural experiment of randomized college roommate assignments in the United States.

Many colleges and universities seek to leverage the promise of intergroup contact theory by adopting housing policies that randomly assign first-year students to roommates, with the goal of increasing intergroup contact. Yet, it is unclear whether random roommate assignment policies increase cross-race contact, whether this (potentially involuntary, but sanctioned by authorities) contact improves racial attitudes or behaviors, or how these effects may differ for racial majority and minority students. The present studies used a natural experiment of random roommate assignment to directly test roommate relationship, attitudinal, and behavioral changes based on roommate race. Across three samples drawn from two student cohorts, the random assignment policy increased the likelihood that students had a cross-race roommate because without the policy, students tended to self-segregate by race. Moreover, selecting (Study 1) or being randomly assigned (Study 2) a cross-race roommate was associated with having more racial outgroup friends and demonstrating more positive verbal and nonverbal behavior during a novel cross-race interaction (Study 3). There were no roommate group (same vs. cross-race roommates) differences in relationship quality, and the results were largely independent of participant race. These findings suggest randomized roommate assignment is a promising avenue for universities to promote cross-race contact amid persistent racial segregation on college campuses with limited negative consequences for relationship quality. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

The NDV-MLS as an Immunotherapeutic Strategy for Breast Cancer: Proof of Concept in Female Companion Dogs with Spontaneous Mammary Cancer.

The absence of tumor-infiltrating lymphocytes negatively impacts the response to chemotherapy and prognosis in all subtypes of breast cancer. Therapies that stimulate a proinflammatory environment may help improve the response to standard treatments and also to immunotherapies such as checkpoint inhibitors. Newcastle disease virus (NDV) shows oncolytic activity, as well as immune modulating potential, in the treatment of breast cancer in vitro and in vivo; however, its potential to enhance tumor-infiltrating immune cells in breast cancer has yet to be evaluated. Since spontaneous canine mammary tumors represent a translational model of human breast cancer, we conducted this proof-of-concept study, which could provide a rationale for further investigating NDV-MLS as immunotherapy for mammary cancer. Six female companion dogs with spontaneous mammary cancer received a single intravenous and intratumoral injection of oncolytic NDV-MLS. Immune cell infiltrates were evaluated by histology and immunohistochemistry in the stromal, intratumoral, and peritumoral compartments on day 6 after viral administration. Increasing numbers of immune cells were documented post-viral treatment, mainly in the peritumoral compartment, where plasma cells and CD3+ and CD3-/CD79- lymphocytes predominated. Viral administration was well tolerated, with no significant adverse events. These findings support additional research on the use of NDV-MLS immunotherapy for mammary cancer.

Parasitism-Induced Changes in Microbial Eukaryotes of Peruvian Alpaca Gastrointestinal Tract.

Alpacas, important genetic resources in the Andean region of Peru, are vulnerable to diarrhea caused by pathogenic parasites such as Eimeria lamae and Giardia sp., which can be fatal, especially in neonates, due to their physiological immaturity and limited adaptability. The study investigated the diversity and abundance of intestinal fungi and protists in alpacas infected with Eimeria lamae and Giardia sp. compared to healthy alpacas. A total of 19 alpacas, aged between one and two months, were included. They were divided into two groups, one with pathological conditions (nine) and the other healthy (ten). Parasitological analyses for the detection of parasites and subsequent molecular analysis were performed on the collected fecal samples. The results revealed a greater diversity and abundance of protists in infected alpacas in comparison with healthy alpacas, while the fungal composition did not show significant changes. Therefore, parasitic infections affect the protist component of the alpaca gut microbiota. Also, it was observed that Blastocystis was identified in all healthy alpacas, serving as a possible marker of the health of the intestinal microbiota; in addition, Prussia and Pichia are beneficial fungi that help control diseases. This groundbreaking study in neonatal alpacas is the first to explore potential changes in the intestinal microbiota during an infectious state, underscoring the importance of further research to comprehend its effects on alpaca health and immune responses.

Study of an Enterococcus faecium strain isolated from an artisanal Mexican cheese, whole-genome sequencing, comparative genomics, and bacteriocin expression.

Enterococci are ubiquitous microorganisms in almost all environments, from the soil we step on to the food we eat. They are frequently found in naturally fermented foods, contributing to ripening through protein, lipid, and sugar metabolism. On the other hand, these organisms are also leading the current antibiotic resistance crisis. In this study, we performed whole-genome sequencing and comparative genomics of an Enterococcus faecium strain isolated from an artisanal Mexican Cotija cheese, namely QD-2. We found clear genomic differences between commensal and pathogenic strains, particularly in their carbohydrate metabolic pathways, resistance to vancomycin and other antibiotics, bacteriocin production, and bacteriophage and CRISPR content. Furthermore, a bacteriocin transcription analysis performed by RT-qPCR revealed that, at the end of the log phase, besides enterocins A and X, two putative bacteriocins not reported previously are also transcribed as a bicistronic operon in E. faecium QD-2, and are expressed 1.5 times higher than enterocin A when cultured in MRS broth.

Exploring the Orgasm Gap Across Racial/Ethnic Groups: A Descriptive Analysis.

Orgasm gaps between heterosexual men and women, and for women across sexual orientations, are well-established in research. However, no large-scale assessments of orgasm frequency by race/ethnicity exist. Here, we analyzed 10 years of cross-sectional Singles in America survey data between 2011 and 2021 to investigate the orgasm gap at the intersection of gender and racial/ethnic identity (i.e. White, Black, Hispanic/Latino, and Asian) for heterosexual participants (N = 27,347). White and Hispanic/Latino men reported greater orgasm frequency than Black and Asian men. Hispanic/Latino women reported the greatest orgasm frequency, and Asian women reported the lowest, with White and Black women's frequencies in between. The orgasm gap between men and women was largest for White (d = 0.89) and Asian (d = 0.86) groups, although Asian participants reported a lower orgasm frequency than White participants overall. The orgasm gap was smaller for Hispanic/Latino participants (d = 0.66), because Hispanic/Latino women reported a greater orgasm frequency than other racial/ethnic groups of women. The orgasm gap was smaller for Black participants (d = 0.61), because Black men reported a lower orgasm frequency than some other racial/ethnic groups of men. This descriptive study serves as an important starting point for future research on orgasm experiences across racial/ethnic groups.

Advanced Oxidative Protein Products Had a Diagnostic Accuracy for Identifying Chronic Kidney Disease in Adult Population.

Chronic Kidney Disease (CKD) is a serious public health problem. Hyperglycemia stimulates the production of reactive oxygen species that cause oxidative damage to proteins. AOPPs constitute a group of oxidized dityrosine-containing proteins that are generated during periods of oxidative stress. They have proved to be a valuable early marker of oxidative tissue damage and active mediators of inflammation associated with the uremic state. To analyze if advanced oxidative protein products (AOPPs) have diagnostic accuracy for identifying chronic kidney disease (CKD) in the adult population. We conducted a diagnostic test validation study in 302 adults ≥20 years old, of both sexes, with and without T2D. After obtaining informed consent, a comprehensive clinical history, anthropometric measurements (weight, BMI) and blood pressure were recorded. Glucose, cholesterol, triglyceride, HDL-c, LDL-c and AOPPs were determinates. Glomerular filtration rate (GFR) was calculated using Cockcroft-Gault (C-G) corrected by body surface area (BSA, mL/min/1.73 m2), CKD-EPI and MDRD equations to identify five stages of CKD. This study follows the Standards for Reporting Diagnostic Accuracy Studies (STARD). The median value of AOPPs was 198.32 µmol/L (minimum-maximum value: 113.48-522.42 µmol/L). The group with patients diagnosed with T2D exhibited higher concentrations (median: 487.39 µmol/L) compared to the non-diabetic group (median: 158.50 µmol/L, p = 0.0001). The selected cut-off point was ≥200 µmol/L using the closest to the median value of AOPPs with sensitivity and specificity as follows: C-G: sensitivity 96.58%; specificity 80%; likelihood ratio: 4.83; CKD-EPI: sensitivity 95.76%; specificity 79.89%; likelihood ratio: 4.76; MDRD: sensitivity 86.55%; specificity: 73.22%; likelihood ratio: 3.23. A difference was observed between AOPPs and chronic kidney disease stage. This study provides evidence that AOPPs ≥ 200 µmol/L have diagnostic accuracy in identifying stage 4-5 CKD by C-G, MDRD and CKD-EPI equations in adults with and without T2D.

Personality traits as identity threat cues: Stigmatized perceivers infer prejudice from disagreeableness.

OBJECTIVE: Across four studies, we examined whether certain personality traits cue prejudice and serve as identity threat cues. BACKGROUND: Stigmatized group members may be vigilant to personality cues that signal prejudice. METHOD: In Study 1 (N = 76), perceivers selected traits and behaviors associated with disagreeableness and closedness to experience as indicators of prejudice. In Studies 2-4, perceivers with stigmatized identities (Total N = 907) learned about a target person who was depicted as disagreeable or agreeable (Studies 2 and 3) and as disagreeable or another trait matched on perceived negativity (i.e., low in conscientiousness, Study 4). RESULTS: Participants perceived the disagreeable target as more discriminatory and hierarchy-endorsing (Studies 2-4), more morally disengaged (Study 3), and more likely to discriminate against stigmatized identity groups (Studies 2 and 4) than the agreeable or low conscientious targets. The relationship between target disagreeableness and perceived discrimination was partially explained by higher perceived hierarchy endorsing beliefs (Studies 2-4) and perceived moral disengagement (Study 3). CONCLUSIONS: This research finds that perceivers with stigmatized identities utilize target disagreeableness as a cue of identity threat, inferring that disagreeable people are more likely to be discriminatory, prejudicial, and hierarchy-endorsing than agreeable and low conscientious people.

"What's Something You've Heard About Sex, But Are Unsure If It's True?": Assessing Middle and High School Students' Sex Education Questions.

PURPOSE: Comprehensive sex education (CSE) encourages safer sex behavior for teens and young adults. However, young people recognize a gap between sex education as taught in the classroom and the reality of their sexual experiences. Thus, CSE should take into account the perspectives of its target population. METHODS: The current project assesses young people's sexual uncertainties by analyzing open-ended responses that were anonymously submitted during in-person sex education sessions. These education programs were administered in middle and high schools in New York State by facilitators from Planned Parenthood Hudson Peconic, Inc. The authors analyzed 1,335 responses from a racially diverse sample of students between the ages of 10 and 21, with 75% of participants between 15 and 17 years old. RESULTS: Using content analysis, the authors identified 49 recurring content codes in participants' responses, which were organized into 16 categories. Most responses centered around pregnancy, sexually transmitted infections, and how to prevent these outcomes. However, responses also highlighted topics that are not always covered with nuance and transparency, if at all, in sex education (e.g., withdrawal, effectiveness of condoms and other contraception, anal and oral sex). Additionally, gender analyses indicated that girls made greater reference to pain, while boys made greater reference to pleasure, which has implications for girls' development of a positive sexuality. DISCUSSION: These results should be interpreted with a social equity lens to inform the development of needs-driven, target-based CSE programming.

Contributions of educational interventions in the management of Computer Vision Syndrome: a review of the literature / Aportes de las intervenciones educativas en el manejo del Síndrome Visual Informático: una revisión de literatura / Contribuições das intervenções educacionais no Manejo da Síndrome da Visão Computacional: uma revisão de literatura

Objective: To identify health education interventions that have demonstrated improvements in the visual and ocular health of computer-using workers with Computer Vision Syndrome. Method: Bibliographic review of original articles in Spanish and English, published between 2017 and June 2022 in PubMed, Scopus, MEDLINE (EBSCO), Redalyc, and Ovid databases. Results: Out of 1,695 articles, 11 complied with the protocol. Of these, 90.90% were published from 2020 to June 2022 and India had the highest number of publications (27.27%). Most of the studies were experimental or quasi-experimental (36.36%), and for the qualitative synthesis they were classified in educational interventions with technology 54.54% (n=6) and those that handled traditional education 45.45% (n=5). Conclusion: Health education interventions were shown to contribute to the improvement of visual and ocular health of computer users with Computer Vision Syndrome. It is necessary to investigate and divulge results about the topic, which contribute to the processes of promotion of visual and ocular health, as well as in the prevention of Computer Vision Syndrome in the workplace

Objetivo: Identificar intervenciones de educación para la salud que hayan demostrado me-joras en la salud visual y ocular de trabajadores usuarios de computadoras con Síndrome de Visión por Computador. Método: Revisión bibliográfica de artículos originales en español e inglés, publicados entre 2017 y junio de 2022 en las bases de datos PubMed, Scopus, MEDLINE (EBSCO), Redalyc y Ovid. Resultados: De 1.695 artículos, 11 cumplieron con el protocolo. De estos, el 90,90% se publicaron desde 2020 hasta junio de 2022 y la India tuvo el mayor número de publicaciones (27,27%). La mayoría de los estudios fueron experimentales o cuasi-experimentales (36,36%), y para la síntesis cualitativa se clasificaron en intervenciones educativas con tecnología 54,54% (n=6) y las que manejaban educación tradicional 45,45% (n=5). Conclusión: Se demostró que las intervenciones de educación en salud contribuyen a la mejora de la salud visual y ocular de los usuarios de computadora con Síndrome de Visión por Computador. Es necesario investigar y divulgar resultados sobre el tema, que contribuyan a los procesos de promoción de la salud visual y ocular, así como en la prevención del Síndrome de Visión por Computador en el ámbito laboral

Objetivo: Identificar intervenções de educação em saúde que demonstraram melhorias na saúde visual e ocular de trabalhadores usuários de computador com Síndrome de Visão de Computa-dor. Método: Revisão bibliográfica de artigos originais em espanhol e inglês, publicados entre 2017 e junho de 2022 nas bases de dados PubMed, Scopus, MEDLINE (EBSCO), Redalyc e Ovid. Resultados: Dos 1.695 artigos, 11 estavam de acordo com o protocolo. Destes, 90,90% foram publicados de 2020 a junho de 2022 e a Índia apresentou o maior número de publicações (27,27%). A maioria dos estudos foi experimental ou quase-experimental (36,36%), e para a síntese qualitativa foram classificados em intervenções educativas com tecnologia 54,54% (n=6) e aquelas que tratavam da educação tradicional 45,45% (n=5). Conclusão: As intervenções de educação em saúde demonstraram contribuir para a melhoria da saúde visual e ocular de usuários de computador com Síndrome de Visão de Computador. É preciso investigar e divulgar resulta-dos sobre o tema, que contribuam nos processos de promoção da saúde visual e ocular, bem como na prevenção da Síndrome da Visão do Computador no ambiente de trabalho

Propiedades psicométricas de la escala de prosocialidad de Caprara et al. (2005) en Colombia

Objetivo: El presente estudio tuvo como objetivo establecer las propiedades psicométricas de la escala de prosocialidad de Caprara et al. (2005) en Colombia, tomando como referencia su adaptación con estudiantes chilenos (Mieres-Chacaltana et al., 2020), dada la reciente relevancia investigativa que ha tenido el comportamiento prosocial en los escenarios educativos y sociales y la ausencia de un instrumento que evalúe este constructo en adolescentes colombianos. Método: Se realizó un análisis de confiabilidad, análisis factorial exploratorio y análisis factorial confirmatorio. Participaron 501 estudiantes universitarios de dos Instituciones de Educación Superior (IES) ubicadas en los departamentos de Magdalena y Tolima con edades comprendidas entre 15 y 18 años (M=17,12 años, DE=0,766), que voluntariamente decidieron vincularse a esta investigación. Resultados: La validez de constructo se estableció mediante un análisis factorial confirmatorio y ajuste del modelo con límites estándares de aceptación: RMSEA=0,070, CFI = 0,923 y TLI = 0,905. El análisis de consistencia de fiabilidad evidenció un índice adecuado de fiabilidad Alfa de Cronbach (α=0,880). Conclusiones: Este instrumento presenta propiedades psicométricas que lo constituyen en una adecuada herramienta para evaluar la prosocialidad en adolescentes colombianos. Su relevancia radica en que permite el empleo de una escala normalizada para Colombia con el fin de medir la prevalencia de comportamientos prosociales reportados por esta población y se une a otras validaciones para poblaciones latinoamericanas ampliando las posibilidades de estudiar este constructo en relación con otras variables propias de la región.

Objective: The present study aimed to establish the psychometric properties of the prosociality scale of Caprara et al. (2005) in Colombia, taking as a reference its adaptation with Chilean students (Mieres-Chacaltana et al., 2020), given the recent research relevance that prosocial behavior has had in educational and social settings and the absence of an instrument that evaluates this construct in Colombian adolescents. Method: A reliability analysis, exploratory factor analysis, and confirmatory factor analysis were performed. 501 university students from two Higher Education Institutions (HEIs) located in the departments of Magdalena and Tolima with ages between 15 and 18 years old (M=17,12 years, SD=0,766) participated, who voluntarily decided to join this research. Results: Construct validity was established through confirmatory factor analysis and model adjustment with standard acceptance limits: RMSEA=0,070, CFI=0,923, and TLI=0,905. The reliability consistency analysis showed an adequate Cronbach's Alpha reliability index (α=0,880). Conclusions: This instrument presents psychometric properties that make it an adequate tool to assess prosociality in Colombian adolescents. Its relevance lies in the fact that it makes it possible to measure the prevalence of prosocial behaviors reported by this population and broadens the possibilities of studying this construct in relation to other variables.

Ingesta dietética y patrón alimentario de las mujeres en periodo de gestación y lactancia de los pueblos indígenas de Colombia. Problemática que requiere urgente atención

Objetivo: Analizar el patrón alimentario y el riesgo de deficiencia en la ingesta usual de energía y nutrientes de las mujeres gestantes y lactantes de algunos pueblos indígenas. Metodología: Estudio descriptivo transversal, con información del componente de ingesta dietética tomada del Estudio nacional de la situación alimentaria y nutricional de los pueblos indígenas de Colombia, realizado entre 2013 y 2019, que incluyó 1028 mujeres indígenas gestantes (319) y lactantes (709), de las regiones norte y sur del país. Resultados: Se encontró una proporción superior al 90 % (Desviación estándar = 0,04) de gestantes y lactantes con consumo inferior al recomendado de calorías (p= 0,038). Alrededor del 70 % de las gestantes y lactantes no consumió lácteos el día anterior a la encuesta, y 50 % no consumió frutas y verduras. Con respecto a los micronutrientes, se encontraron, en las gestantes y lactantes, altas prevalencias del riesgo de deficiencia en la ingesta de vitamina C (50,3 y 80,2 %), folatos (80,4 y 95,1 %), zinc (87,6 y 96,3 %), hierro (88,9 y 68,6 %) y calcio (87,5 y 98,5 %). Conclusión: El consumo de alimentos y nutrientes en las mujeres indígenas gestantes y lactantes es deficiente en nutrientes claves para mantener su estado de salud y proporcionar los nutrientes necesarios a su bebé, y su patrón alimentario se clasifica como no saludable.

Objective: To analyze the dietary pattern and deficiency risk in the usual energy and nutrient intake of pregnant and lactating women from some indigenous peoples. Methodology: Cross-sectional descriptive study, with information from the dietary intake component taken from the National Survey of the food and nutritional situation of indigenous peoples in Colombia, conducted between 2013 and 2019, which included 1,028 pregnant (319) and lactating (709) indigenous women from the northern and southern regions of the country. Results: A proportion higher than 90% (SD = 0.04) of pregnant and lactating women with lower than recommended calorie intake was found (p = 0.038). Around 70% of pregnant and lactating women had no any dairy products the day before the survey, and 50% did not eat fruits and vegetables. Regarding micronutrients, high prevalence of deficiency risk in the intake of vitamin C (50.3 and 80.2%), folate (80.4 and 95.1%), zinc (87.6 and 96.3%), iron (88.9 and 68.6%) and calcium (87.5 and 98.5%) was found in pregnant and lactating women. Conclusion: Food and nutrient intake in pregnant and lactating indigenous women is deficient in key nutrients to maintain their general health and provide the necessary nutrients to their babies. Their dietary pattern is considered unhealthy.

Objetivo: Analisar o padrão alimentar e o risco de deficiência na ingestão usual de energia e nutrientes das mulheres gestantes e lactantes de alguns povos indígenas. Metodologia: Estudo descritivo transversal, com informação do componente de ingesta dietética tirada do Estudo nacional da situação alimentar e nutricional dos povos indígenas da Colômbia, realizado entre 2013 e 2019, que incluiu 1028 mulheres indígenas gestantes (319) e lactantes (709) das regiões norte e sul do país. Resultados: Encontrou-se uma proporção superior a 90% (Desvio-padrão = 0,04) de gestantes e lactantes com consumo inferior ao recomendado em calorias (p = 0,038). Ao redor de 70% das gestantes e lactantes não consumiu lácteos no dia prévio à enquete, e 50% não consumiu frutas e verduras. No que se refere aos micronutrientes, acharam-se nas gestantes e lactantes altas prevalências de risco de deficiência na ingestão de vitamina C (50,3 e 80,2 %), folatos (80,4 e 95,1 %), zinco (87,6 e 96,3 %), ferro (88,9 e 68,6 %) e cálcio (87,5 e 98,5 %). Conclusão: O consumo de alimentos e nutrientes nas mulheres indígenas gestantes e lactantes é deficiente em nutrientes-chave para manter seu estado de saúde e proporcionar os nutrientes necessários para o bebê; seu padrão alimentar classifica-se como não saudável.

Aplicaciones de un instrumento para evaluar factores de riesgo comportamentales asociados a enfermedades no transmisibles en población trabajadora / Applications of an instrument to assess behavioral risk factors associated with non-communicable diseases in the working population

Introducción: La Organización Mundial de la Salud diseñó un instrumento para la vigilancia de los factores de riesgo asociados con las enfermedades crónicas, este abarca tres pasos que incluyen los datos personales y comportamentales, antropométricos y bioquímicos. Objetivo: Describir las aplicaciones del Instrumento STEPS (paso 1) para la identificación de factores de riesgo comportamentales asociados a enfermedades no transmisibles en adultos trabajadores, según país y año de publicación. Metodología: Revisión narrativa de estudios originales que utilizaron el STEPS, publicados entre 2015 y 2019 en español, inglés y portugués en las bases de datos PubMed, SciELO y el motor de búsqueda Google Scholar. Resultados: Se revisaron 20 documentos de los cuales 6 fueron de Asia y África, y el año de mayor publicación fue 2017. El consumo nocivo del alcohol, tabaquismo, inactividad física y alimentación no saludable son los riesgos más estudiados. Conclusiones: La utilización del instrumento STEPS en publicaciones es más frecuente en países de África y Asia. Se requiere que su implementación sea sistemática y difundida en otros países, con la finalidad de diseñar e implementar estrategias de salud pública para la prevención, mitigación y tratamiento oportuno de las enfermedades no transmisibles.

Introduction: The World Health Organization designed an instrument for the surveillance of risk factors associated with chronic diseases, it has three steps that include personal and behavioral, anthropometric, and biochemical data. Objective: To describe the applications of the STEPS Instrument (step 1) to identify behavioral risk factors associated with noncommunicable diseases in working adults, according to country and year of publication. Methodology: Narrative review of original studies that used STEPS, published between 2015 and 2019 in Spanish, English and Portuguese in the databases PubMed, SciELO, and the search engine Google Scholar. Results: A total of 20 studies were reviewed, of which 6 were from Asia and Africa, and the year of most significant publication was 2017. The most studied risks are harmful alcohol consumption, smoking, physical inactivity, and unhealthy eating. Conclusions: the use of the STEPS instrument in publications is more frequent in countries of Africa and Asia. Its implementation must be systematic and disseminated in other countries to design and implement public health strategies for the prevention, mitigation, and timely treatment of noncommunicable diseases.

[Autoimmunity and Freiburg classification in common variable immunodeficiency]. / Autoinmunidad y clasificación de Freiburg en pacientes con inmunodeficiencia común variable.

Introduction: Up to 25% of patients with common variable immunodeficiency (CVID) debut with autoimmunity, which is related to the Freiburg classification, which is based on flow cytometry. Objective: to determine the frequency and type of autoimmune diseases and their association with the Freiburg classification in adults with CVID. Methods: A cross-sectional, analytical and observational study was carried out with 33 patients belonging to the Primary Immunodeficiency Clinic of a third level hospital, with a diagnosis of CVID. They were divided into 3 phenotypes according to the Freiburg classification. Results: Of the 33 patients studied, 66.6% presented autoimmune diseases, 19 of them (86.3%) had cytopenia; 42.1% belonged to Freiburg group Ia, 36.8% to Ib and 21% to phenotype II. In 36.6% of the patients, autoimmune cytopenia were the first manifestation of CVID; and up to 70% of them belong to the Freiburg phenotype Ia (p = 0.086). Patients with autoimmune cytopenia had a lower percentage of isotype-switched memory B cells (p = 0.018), no higher percentage of CD21low B cells (p = 0.226). Conclusions: Classification by CVID phenotypes allows the identification of the patient's profile according to the percentage of memory B cells with isotype change, which is useful to intentionally search for non-infectious complications of the disease.

Introducción: hasta el 25% de los pacientes con inmunodeficiencia común variable (IDCV) debutan con autoinmunidad, la cual guarda relación con la clasificación de Freiburg, que se basa en la citometría de flujo. Objetivo: determinar la frecuencia y tipo de enfermedades autoinmunes y su asociación con la clasificación de Freiburg en adultos con IDCV. Métodos: se realizó un estudio transversal, analítico y observacional con 33 pacientes pertenecientes a la Clínica de Inmunodeficiencias Primarias de un hospital de tercer nivel con diagnóstico de IDCV. Se dividieron en tres fenotipos según la clasificación de Freiburg. Resultados: de los 33 pacientes estudiados, el 66.6% presentó enfermedades autoinmunes, de ellos 19 (86.3%) tuvieron citopenias. El 42.1% se clasificó en el grupo Ia de Freiburg, el 36.8% en el grupo Ib y el 21% en el fenotipo II. En el 36.6% de los pacientes las citopenias autoinmunes fueron la primera manifestación de IDCV, y hasta el 70% de ellos pertenecen al fenotipo Ia de Freiburg (p = 0.086). Los pacientes con citopenias autoinmunes tuvieron un menor porcentaje de células B de memoria con cambio de isotipo (p = 0.018), sin mayor porcentaje de células B CD21low (p = 0.226). Conclusiones: la clasificación por fenotipos en IDCV permite identificar el perfil del paciente y el tipo de manifestaciones asociadas, lo que es útil para buscar de manera intencionada complicaciones no infecciosas propias de la enfermedad.

Intensity-modulated radiotherapy and chemotherapy for canine right atrial tumors: A retrospective study of seven dogs.

Most primary cardiac tumors in dogs are located in the right atrium/atrial appendage, with hemangiosarcoma being the most common. The aims of this retrospective, case series were to describe outcomes for seven dogs with right atrial tumors treated with hypofractionated intensity-modulated radiotherapy and concurrent vinblastine and propranolol. One dog had a complete response, four dogs had partial responses and two dogs had stable disease after treatment. Effusions resolved in all dogs. Median progression-free survival was 290 days. Five dogs died from metastatic disease, one dog from unrelated neoplasia, and one dog is alive. Median overall survival was 326 days. Three dogs with confirmed hemangiosarcoma survived 244, 326, and 445 days. Two dogs developed clinically significant, but nonfatal, cardiac arrhythmias. One dog that received three courses of radiation had subclinical myocardial and arterial fibrosis at necropsy. Hypofractionated chemoradiotherapy was well tolerated and may provide clinical benefit in dogs with right atrial tumors.