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1.
Environ Toxicol Chem ; 29(3): 522-34, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20821474

RESUMO

River sediments are a dynamic system, especially in areas where floods occur frequently. In the present study, an integrative approach is used to investigate the seasonal and spatial dynamics of contamination of sediments from a regularly flooded industrial area in the Czech Republic, which presents a suitable model ecosystem for pollutant distribution research at a regional level. Surface sediments were sampled repeatedly to represent two different hydrological situations: spring (after the peak of high flow) and autumn (after longer period of low flow). Samples were characterized for abiotic parameters and concentrations of priority organic pollutants. Toxicity was assessed by Microtox test; genotoxicity by SOS-chromotest and green fluorescent protein (GFP)-yeast test; and the presence of compounds with specific mode of action by in vitro bioassays for dioxin-like activity, anti-/androgenicity, and anti-/estrogenicity. Distribution of organic contaminants varied among regions and seasonally. Although the results of Microtox and genotoxicity tests were relatively inconclusive, all other specific bioassays led to statistically significant regional and seasonal differences in profiles and allowed clear separation of upstream and downstream regions. The outcomes of these bioassays indicated an association with concentrations of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) as master variables. There were significant interrelations among dioxin-like activity, antiandrogenicity and content of organic carbon, clay, and concentration of PAHs and PCBs, which documents the significance of abiotic factors in accumulation of pollutants. The study demonstrates the strength of the specific bioassays in indicating the changes in contamination and emphasizes the crucial role of a well-designed sampling plan, in which both spatial and temporal dynamics should be taken into account, for the correct interpretations of information in risk assessments.


Assuntos
Sedimentos Geológicos/análise , Rios/química , Poluentes Químicos da Água/análise , Aliivibrio fischeri/efeitos dos fármacos , Bioensaio , Compostos Orgânicos/análise , Bifenilos Policlorados/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , Estações do Ano , Poluentes Químicos da Água/toxicidade
2.
Biosens Bioelectron ; 23(12): 1850-5, 2008 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-18403193

RESUMO

Sediments polluted with high concentrations of persistent organic pollutants, many of which are ligands of the aryl hydrocarbon receptor (AhR), are currently of concern around the industrialized world. Bioassays that can detect the presence of AhR ligands in environmental samples offer a relatively rapid and cost-effective means of prioritizing samples before more elaborate, laborious, and costly chemical analyses are applied. This paper presents a new bioluminescent yeast assay based on transcriptional activation of AhR. Its applicability for determining AhR ligands in complex environmental samples was demonstrated by analyzing a set of sediment samples from the River Kymi, Finland. The results from the assay are shown to be consistent with those from both a chemical analysis and an H4IIE-luc bioassay. The yeast assay procedure is simple and can be performed within 1 day. The yeasts grow rapidly, are easy to handle, and do not require continuous cell culturing. Moreover, the robustness of the yeast allows the application of the test to crude extracts or even sediment suspensions. The yeast assay described in this paper can be useful in screening and prioritization of samples prior to chemical analysis. Moreover, the strain can be used in the construction of fibre-optic biosensors.


Assuntos
Bioensaio/instrumentação , Sedimentos Geológicos/análise , Medições Luminescentes/instrumentação , Receptores de Hidrocarboneto Arílico/análise , Receptores de Hidrocarboneto Arílico/química , Saccharomyces cerevisiae/isolamento & purificação , Poluentes do Solo/análise , Desenho de Equipamento , Análise de Falha de Equipamento , Ligantes , Luminescência , Saccharomyces cerevisiae/metabolismo
3.
Environ Toxicol Chem ; 26(8): 1591-9, 2007 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17702330

RESUMO

Retinoids are known to regulate important processes such as differentiation, development, and embryogenesis. Some effects, such as malformations in frogs or changes in metabolism of birds, could be related to disruption of the retinoid signaling pathway by exposure to organic contaminants. A new reporter gene assay has been established for evaluation of the modulation of retinoid signaling by individual chemicals or environmental samples. The bioassay is based on the pluripotent embryonic carcinoma cell line P19 stably transfected with the firefly luciferase gene under the control of a retinoic acid-responsive element (clone P19/ A15). The cell line was used to characterize the effects of individual chemicals and sediments extracts on retinoid signaling pathways. The extracts of sediments from the River Kymi, Finland, which contained polychlorinated dioxins and furans and polycyclic aromatic hydrocarbons (PAHs), significantly increased the potency of all-trans retinoic acid (ATRA), while no effect was observed with the extract of the sediment from reference locality. Considerable part of the effect was caused by the labile fraction of the sediment extracts. Also, several individual PAHs potentiated the effect of ATRA; on the other hand, 2,3,7,8-tetrachlorodibenzo-p-dioxin and several phthalates showed slightly inhibiting effect. These results suggest that PAHs could be able to modulate the retinoid signaling pathway and that they could be responsible for a part of the proretinoid activity observed in the sediment extracts. However, the effects of PAHs on the retinoic acid signaling pathways do not seem to be mediated directly by crosstalk with aryl hydrocarbon receptor.


Assuntos
Bioensaio/métodos , Poluentes Ambientais/toxicidade , Sedimentos Geológicos/química , Retinoides/metabolismo , Transdução de Sinais , Animais , Carcinoma Embrionário/patologia , Linhagem Celular Tumoral , Dioxinas/análise , Dioxinas/toxicidade , Poluentes Ambientais/análise , Finlândia , Furanos/análise , Furanos/toxicidade , Genes Reporter , Luciferases de Vaga-Lume/genética , Ácidos Ftálicos/análise , Ácidos Ftálicos/toxicidade , Hidrocarbonetos Policíclicos Aromáticos/análise , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Retinoides/genética , Medição de Risco , Tretinoína/análise , Tretinoína/toxicidade
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