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Pathog Dis ; 79(7)2021 09 06.
Artigo em Inglês | MEDLINE | ID: mdl-34410374

RESUMO

Neisseria meningitis (Nm) is a human-specific bacterial pathogen that can cause sepsis and meningitis. To cause meningitis Nm must enter the central nervous system (CNS) across one of the barriers between the blood and the brain. We have previously shown that a capsule-depleted Serogroup B strain of Nm displays enhanced invasion into human choroid plexus (CP) epithelial papilloma (HIBCPP) cells, which represent an in vitro model of the blood-cerebrospinal fluid barrier (BCSFB). Still, the processes involved during CNS invasion by Nm, especially the role of host cell actin cytoskeleton remodeling, are not investigated in detail. Here, we demonstrate that invasion into CP epithelial cells by encapsulated and capsule-depleted Nm is mediated by distinct host cell pathways. Whereas a Serogroup B wild-type strain enters HIBCPP cells by a possibly dynamin-independent, but actin related protein 2/3 (Arp2/3)-dependent mechanism, invasion by a capsule-depleted mutant is reduced by the dynamin inhibitor dynasore and Arp2/3-independent. Both wild-type and mutant bacteria require Src kinase activity for entry into HIBCPP cells. Our data show that Nm can employ different mechanisms for invasion into the CP epithelium dependent on the presence of a capsule.


Assuntos
Complexo 2-3 de Proteínas Relacionadas à Actina/metabolismo , Cápsulas/metabolismo , Dinaminas/metabolismo , Células Epiteliais/microbiologia , Infecções Meningocócicas/metabolismo , Infecções Meningocócicas/microbiologia , Neisseria meningitidis/metabolismo , Actinas/metabolismo , Barreira Hematoencefálica/metabolismo , Barreira Hematoencefálica/microbiologia , Células Cultivadas , Plexo Corióideo/metabolismo , Plexo Corióideo/microbiologia , Endocitose , Células Epiteliais/metabolismo , Epitélio/metabolismo , Epitélio/microbiologia , Interações Hospedeiro-Patógeno , Humanos , Neisseria meningitidis/patogenicidade , Transdução de Sinais , Virulência , Quinases da Família src/metabolismo
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