GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS / Variabilidad genética y características reproductivas de poblaciones de pez cebra (Cyprinidae Danio rerio)

ABSTRACT Specimens of cultured zebrafish acquired from different fish farms in Brazil may show genetic variability and alteration in allele frequency due to genetic drift and selective pressure in a captive environment, resulting in the differentiation of productive and reproductive characteristics. The aim of this study was to evaluate the genetic variability and reproductive characteristics of 180 zebrafish specimens from six Brazilian fish farms. A deviation from the Hardy-Weinberg equilibrium was observed in all evaluated stocks. Differentiation among stocks was observed in the amount of genetic variability with respect to observed heterozygosity and the inbreeding coefficient (FIS). Genetic distance between stocks was determined through the Fst index, and the formation of four distinct groups was observed by plotting the dendrogram based on Nei's genetic distance. Differences were observed among reproductive parameters, such as the average number of eggs per female and hatchability. This second parameter proved to be related to the level of inbreeding of the population, whereas this effect was not observed for spawning frequency. We conclude that zebrafish stocks from the 6 different Brazilian fish farms present significant genetic and phenotypic variability. The genetic structure affects fecundity and should be considered when carrying out work where reproductive rates are evaluated.

RESUMEN Especímenes de pez cebra adquiridos en diferentes piscifactorías pueden mostrar variabilidad genética y alteración en la frecuencia de los alelos debido a la deriva genética y presión selectiva llevada a cabo en un ambiente cautivo, lo que resulta en la diferenciación de las características productivas y reproductivas. Este estudio busco evaluar la variabilidad genética y las características reproductivas de 180 especímenes de pez cebra adquiridos de seis piscifactorías brasileras. Hubo una desviación en el equilibrio de Hardy-Weinberg en todas las poblaciones evaluadas. Se encontró diferenciación en términos del grado de variabilidad dentro de las poblaciones, en vista de los resultados de la heterocigosidad observada y el coeficiente de endogamia (Fis). La distancia genética entre ellos se verificó usando el índice Fst, y se observó la formación de cuatro grupos distintos al trazar el dendrograma basado en la distancia genética de Nei. Se observó una diferencia en relación con los parámetros reproductivos, como el número promedio de huevos por hembra y la incubabilidad. Este segundo parámetro demostró estar relacionado con el nivel de consanguinidad de la población, y este efecto no se verificó para la frecuencia de desove. Se puede considerar que las existencias de pez cebras de diferentes lugares tienen variabilidad genética y fenotípica. La estructura genética influye principalmente en la fertilización y debe tenerse en cuenta al realizar trabajos donde se evalúan los índices reproductivos.

Zebrafish breeding program: genetic parameters estimates for growth traits.

The objective of this study was to evaluate the genetic parameters of two generations of zebrafish breeding program. The base population was formed by crossing individuals of six commercial stocks of zebrafish, resulting in a nucleus with 60 families. Two generations were evaluated, with a total of 780 and 781 individuals for the first and second generation, respectively. The selection was made based on the mean genetic value of each family, followed by mass selection of the breeders. Mathematical models that considered the fixed (age, density in the larval stage, sex, and generation) and random (animal additive genetics, common to full-sibs, and residual) effects were evaluated using BLUPF90 program family. Weight and total length were used as response variables. Total length was the best selection criterion because it had a higher heritability (0.30) than weight (0.22). There was a high common to full-sib effect, especially in the first generation of animals. For second-generation data, the heritability was 0.26 for total length, as well as a lower common to full-sib effect for length. The best model obtained for this evaluation was considering all effects, being age and density as first and second polynomial, respectively. The genetic and phenotypic correlations for weight and length were 0.87 and 0.75, respectively. These results indicate that genetic breeding using total length as the selection criterion may produce a larger and heavier zebrafish strain.

Consumer profile and acceptability of cooked beef steaks with edible and active coating containing oregano and rosemary essential oils.

Fresh animal products are highly perishable and characterized by a short shelf-life. Edible coatings with natural antioxidants (essential oils: EOs) could improve stability, ensure quality, and increase the shelf-life of fresh products. Due to the strong flavor of EOs, their use should consider consumer preferences and sensory acceptability. This study evaluated the effects of edible coating (with oregano and rosemary essential oil) on beef in relation to consumer preferences, besides the determination of habits of consumption and buying intentions of consumers. Acceptability scores from three clusters of consumers was described. Coating with oregano was the preferred. The higher consumer acceptance and willingness to buy this product indicate a great potential and possibility of using coatings with essential oils in fresh animal products.

Genetic parameters for growth performance, fillet traits, and fat percentage of male Nile tilapia (Oreochromis niloticus).

Improvement of fillet traits and flesh quality attributes are of great interest in farmed tilapia and other aquaculture species. The main objective of this study was to estimate genetic parameters for fillet traits (fillet weight and fillet yield) and the fat content of fillets from 1136 males combined with 2585 data records on growth traits (body weight at 290 days, weight at slaughter, and daily weight gain) of 1485 males and 1100 females from a third generation of the Aquaamerica tilapia strain. Different models were tested for each trait, and the best models were used to estimate genetic parameters for the fat content, fillet, and growth traits. Genetic and phenotypic correlations were estimated using two-trait animal models. The heritability estimates were moderate for the fat content of fillets and fillet yield (0.2-0.32) and slightly higher for body weight at slaughter (0.41). The genetic correlation between fillet yield and fat was significant (0.6), but the genetic correlations were not significant between body weight and fillet yield, body weight and fat content, daily weight gain and fillet yield, and daily weight gain and fat content (- 0.032, - 0.1, - 0.09, and - 0.4, respectively). Based on the genetic correlation estimates, it is unlikely that changes in fillet yield and fat content will occur when using growth performance as a selection criterion, but indirect changes may be expected in fat content if selecting for higher fillet yield.

Glúten de trigo em dietas para tilápia do Nilo: digestibilidade e desempenho produtivo de larvas / Wheat gluten in the diet of Nile tilapia:digestibility and larvae growth performance

O objetivo do presente trabalho foi avaliar o potencial de utilização do glúten de trigo em dietas para tilápia-do-nilo (Orechromisniloticus), através de estudos de digestibilidade e desempenho produtivo de larvas. Para o estudo de digestibilidade foi elaboradauma dieta referência e uma dieta teste, contendo glúten de trigo. Utilizou-se o óxido de crômio III como marcador inerte. As fezescoletadas foram destinadas à análise de composição química, assim como as rações e o ingrediente teste. Além disso, foi realizadaanálise de leitura de óxido de crômio das fezes e das dietas. Os resultados obtidos por meio dessas análises foram utilizadospara calcular os coeficientes de digestibilidade. Com base nesses coeficientes foram elaboradas as dietas experimentais dosestudos de dose-resposta para larvas. Foram formuladas cinco dietas experimentais com diferentes níveis de inclusão de glútende trigo, sendo de 0%; 2,5%; 5,0%, 7,5% e 10%. Ao final do experimento foram avaliados o desempenho produtivo dos animais.Os dados de desempenho foram submetidos à análise de regressão linear a 5% de probabilidade. O glúten de trigo apresentaelevados coeficientes de digestibilidade para matéria seca, proteína bruta e energia bruta, sendo de 94,02; 98,18 e 96,90%,respectivamente. A análise de regressão demonstrou um efeito linear negativo quanto ao comprimento final e sobrevivência daslarvas conforme aumentou-se o nível desse alimento. Dessa forma, conclui-se que o glúten de trigo é um ingrediente potencialpara ser utilizado em dietas para tilápia-do-nilo, não sendo recomendada sua inclusão na fase larval.(AU)

The objective of this study was to evaluate the utilization potential of wheat gluten in diets for Nile tilapia (Oreochromis niloticus)through studies of digestibility and productive performance of larvae. For the digestibility study was elaborated a reference dietand a test diet containing wheat gluten. Chromium oxide-III was used as an inert marker. The collected faeces were destined toanalysis of chemists compounds as the feed, and the test ingredient. Besides, reading chromium oxide analysis was performed in thefaeces and diets. The results obtained through these analyses were used to calculate the digestibility coefficients. Based on thesecoefficients, experimental diets were elaborated for the dose-response studies in larvae. For the experiment, five experimental dietswere formulated with different inclusion levels of wheat gluten, with 0%; 2,5%; 5,0%, 7,5% and 10%. At the end of the experiment,the productive performance was evaluated. The performance data were submitted to linear regression with 5% of probability. Thewheat gluten presents high digestibility coefficients for dry matter, crude protein and gross energy with 94,02; 98.18 and 96.90%,respectively. The regression analyses showed a negative linear effect on the final length and survival of the larvae when the levelof this food was increased. Thus, it is concluded that wheat gluten is a potential ingredient to be utilized in diets for Nile tilapia, notbeing recommended its inclusion in the larvae period.(AU)

Glúten de trigo em dietas para tilápia do Nilo: digestibilidade e desempenho produtivo de larvas / Wheat gluten in the diet of Nile tilapia: digestibility and larvae growth performance

O objetivo do presente trabalho foi avaliar o potencial de utilização do glúten de trigo em dietas para tilápia-do-nilo (Orechromis niloticus), através de estudos de digestibilidade e desempenho produtivo de larvas. Para o estudo de digestibilidade foi elaborada uma dieta referência e uma dieta teste, contendo glúten de trigo. Utilizou-se o óxido de crômio III como marcador inerte. As fezes coletadas foram destinadas à análise de composição química, assim como as rações e o ingrediente teste. Além disso, foi realizada análise de leitura de óxido de crômio das fezes e das dietas. Os resultados obtidos por meio dessas análises foram utilizados para calcular os coeficientes de digestibilidade. Com base nesses coeficientes foram elaboradas as dietas experimentais dos estudos de dose-resposta para larvas. Foram formuladas cinco dietas experimentais com diferentes níveis de inclusão de glúten de trigo, sendo de 0%; 2,5%; 5,0%, 7,5% e 10%. Ao final do experimento foram avaliados o desempenho produtivo dos animais. Os dados de desempenho foram submetidos à análise de regressão linear a 5% de probabilidade. O glúten de trigo apresenta elevados coeficientes de digestibilidade para matéria seca, proteína bruta e energia bruta, sendo de 94,02; 98,18 e 96,90%, respectivamente. A análise de regressão demonstrou um efeito linear negativo quanto ao comprimento final e sobrevivência das larvas conforme aumentou-se o nível desse alimento. Dessa forma, conclui-se que o glúten de trigo é um ingrediente potencial para ser utilizado em dietas para tilápia-do-nilo, não sendo recomendada sua inclusão na fase larval.

The objective of this study was to evaluate the utilization potential of wheat gluten in diets for Nile tilapia (Oreochromis niloticus) through studies of digestibility and productive performance of larvae. For the digestibility study was elaborated a reference diet and a test diet containing wheat gluten. Chromium oxide-III was used as an inert marker. The collected faeces were destined to analysis of chemists compounds as the feed, and the test ingredient. Besides, reading chromium oxide analysis was performed in the faeces and diets. The results obtained through these analyses were used to calculate the digestibility coefficients. Based on these coefficients, experimental diets were elaborated for the dose-response studies in larvae. For the experiment, five experimental diets were formulated with different inclusion levels of wheat gluten, with 0%; 2,5%; 5,0%, 7,5% and 10%. At the end of the experiment, the productive performance was evaluated. The performance data were submitted to linear regression with 5% of probability. The wheat gluten presents high digestibility coefficients for dry matter, crude protein and gross energy with 94,02; 98.18 and 96.90%, respectively. The regression analyses showed a negative linear effect on the final length and survival of the larvae when the level of this food was increased. Thus, it is concluded that wheat gluten is a potential ingredient to be utilized in diets for Nile tilapia, not being recommended its inclusion in the larvae period.

Effect of different periods of pre-slaughter stress on the quality of the Nile tilapia meat

Abstract The study was carried out with the objective of evaluating the influence of pre-slaughter stress on meat quality of Nile tilapia (O. niloticus) for 10 days of storage in ice. 75 whole fish were used eviscerated, with average weight and length of 825.75 ± 101.59 g and 28.25 ± 0.81 cm, respectively. The treatments consisted of time in which the animals underwent pre-slaughter hypoxia, being 0, 3 and 6 minutes. Rigor mortis and muscle pH were assessed besides the application of the Quality Index Method and analysis of total protein and blood glucose. Rigor mortis did not differ among treatments during the storage period, and after three hours all animals were in rigor stage. The muscle pH values varied widely during the study period, observing higher values on the third day of storage. Sensory analysis showed that after 10 days the tilapia from the different treatments did not differ in the evaluated quality parameters similarly, there was no difference regarding the evaluated blood biochemical parameters Thus, hypoxia of 6 minutes pre-slaughter does not influence the rigor mortis, muscle pH, qualitative perception of the meat quality, total protein and blood glucose of Nile tilapia.

Tilapia by-product hydrolysate powder in diets for Nile tilapia larvae

This study aimed to evaluate the digestibility of tilapia by-product protein hydrolysate powder (TBHP) from tilapia filleting by-product, and its inclusion in diets for Nile tilapia larvae. In order to determine the apparent digestibility coefficients, two diets were formulated, the reference diet and the test diet (20% of TBHP) and, to evaluate the inclusion, six diets were formulated, which were with 0.0, 2.0; 4.0; 6.0, and 8.0% of TBHP. The apparent digestibility coefficients of crude protein and gross energy were 89.5 and 98.3%, respectively. At levels above 4.0% of inclusion of TBHP, the weight, final length and weight gain were impaired, however, survival rate was higher (p < 0.05). The TBHP can be used in diets for tilapia due to the high digestibility coefficients for crude protein and gross energy. The inclusion of up to 4.0% of TBHP does not affect the reproductive performance and survival rate of Nile tilapia larvae.  

Tilapia by-product hydrolysate powder in diets for Nile tilapia larvae / Hidrolisado proteico seco do resíduo da filetagem de tilápias em dietas para larvas de tilápia do Nilo

This study aimed to evaluate the digestibility of tilapia by-product protein hydrolysate powder (TBHP) from tilapia filleting by-product, and its inclusion in diets for Nile tilapia larvae. In order to determine the apparent digestibility coefficients, two diets were formulated, the reference diet and the test diet (20% of TBHP) and, to evaluate the inclusion, six diets were formulated, which were with 0.0, 2.0; 4.0; 6.0, and 8.0% of TBHP. The apparent digestibility coefficients of crude protein and gross energy were 89.5 and 98.3%, respectively. At levels above 4.0% of inclusion of TBHP, the weight, final length and weight gain were impaired, however, survival rate was higher (p 0.05). The TBHP can be used in diets for tilapia due to the high digestibility coefficients for crude protein and gross energy. The inclusion of up to 4.0% of TBHP does not affect the reproductive performance and survival rate of Nile tilapia larvae.

Objetivou-se avaliar a digestibilidade do hidrolisado proteico seco do resíduo da filetagem de tilápias (HPST) e sua inclusão em dietas para larvas de tilápia do Nilo. Para determinar os coeficientes de digestibilidade aparente, duas dietas foram elaboradas, referência e teste (com 20% do HPST) e para avaliação da inclusão, seis dietas foram formuladas com 0,0, 2,0; 4,0; 6,0, e 8,0% de HPST. Os coeficientes de digestibilidade aparente da proteína e energia bruta foram de 89,5 e 98,3%, respectivamente. Nos níveis acima de 4,0% de inclusão de HPST o peso e comprimento final e ganho em peso foram prejudicados, no entanto a sobrevivência foi maior (p 0,05). O HPST pode ser utilizado em dietas para tilápias em função dos elevados coeficientes de digestibilidade para proteína bruta e energia bruta. A inclusão de até 4,0% de HPST não afeta o desempenho produtivo e a sobrevivência de larvas de tilápias do Nilo.

Tilapia by-product hydrolysate powder in diets for Nile tilapia larvae / Hidrolisado proteico seco do resíduo da filetagem de tilápias em dietas para larvas de tilápia do Nilo

This study aimed to evaluate the digestibility of tilapia by-product protein hydrolysate powder (TBHP) from tilapia filleting by-product, and its inclusion in diets for Nile tilapia larvae. In order to determine the apparent digestibility coefficients, two diets were formulated, the reference diet and the test diet (20% of TBHP) and, to evaluate the inclusion, six diets were formulated, which were with 0.0, 2.0; 4.0; 6.0, and 8.0% of TBHP. The apparent digestibility coefficients of crude protein and gross energy were 89.5 and 98.3%, respectively. At levels above 4.0% of inclusion of TBHP, the weight, final length and weight gain were impaired, however, survival rate was higher (p 0.05). The TBHP can be used in diets for tilapia due to the high digestibility coefficients for crude protein and gross energy. The inclusion of up to 4.0% of TBHP does not affect the reproductive performance and survival rate of Nile tilapia larvae.(AU)

Objetivou-se avaliar a digestibilidade do hidrolisado proteico seco do resíduo da filetagem de tilápias (HPST) e sua inclusão em dietas para larvas de tilápia do Nilo. Para determinar os coeficientes de digestibilidade aparente, duas dietas foram elaboradas, referência e teste (com 20% do HPST) e para avaliação da inclusão, seis dietas foram formuladas com 0,0, 2,0; 4,0; 6,0, e 8,0% de HPST. Os coeficientes de digestibilidade aparente da proteína e energia bruta foram de 89,5 e 98,3%, respectivamente. Nos níveis acima de 4,0% de inclusão de HPST o peso e comprimento final e ganho em peso foram prejudicados, no entanto a sobrevivência foi maior (p 0,05). O HPST pode ser utilizado em dietas para tilápias em função dos elevados coeficientes de digestibilidade para proteína bruta e energia bruta. A inclusão de até 4,0% de HPST não afeta o desempenho produtivo e a sobrevivência de larvas de tilápias do Nilo.(AU)

Artemia sp IN FEEDING LARVAE OF SILVER CATFISH (Rhamdia quelen) / Artemia sp. NA ALIMENTAÇÃO DE LARVAS DE JUNDIÁ (Rhamdia quelen)

A considerable problem in the catfish larvae rearing is related to the first feeding. This study aimed to determine the larvae growth and the survival of silver catfish submitted to feeding with Artemia sp. in different time periods. The study was conducted in the Aquaculture Laboratory of the Universidade Estadual do Oeste do Paraná, with four treatments and five replications, constituted by feeding with Artemia sp. in 5, 10, 15 and 20 days, and after  each period of time, by its replacement by ration. We used 200 larvae distributed into 20 aquariums, soon after the eggs hatched. The larvae were fed 4 times a day until apparent satiation for 30 days. The Artemia sp. feeding for 15 days provided the best results on weight gain, final length and survival.KEYWORDS: Aquaculture; Iguaçu river; native fish; nutrition.

Uma das dificuldades na larvicultura do jundiá está relacionada à primeira alimentação. Este experimento teve como objetivo determinar o crescimento e a sobrevivência de larvas de jundiá submetidas à alimentação com Artemia sp. em diferentes períodos de tempo. O estudo foi realizado no Laboratório de Aquicultura da Universidade Estadual do Oeste do Paraná, com quatro tratamentos e cinco repetições, constituídos pela alimentação com Artemia sp em 5, 10, 15 e 20 dias, e após cada período, por sua substituição pela ração. Foram utilizadas 200 larvas distribuídas em 20 aquários, logo após a eclosão dos ovos. As larvas foram alimentadas 4 vezes ao dia até a saciedade aparente por um período de 30 dias. A alimentação com Artemia sp. durante 15 dias proporcionou os melhores resultados de ganho de peso, comprimento final e sobrevivência.PALAVRAS-CHAVE: Aquicultura; nutrição; peixe nativo; rio Iguaçu.

Artemia sp IN FEEDING LARVAE OF SILVER CATFISH (Rhamdia quelen) / Artemia sp. NA ALIMENTAÇÃO DE LARVAS DE JUNDIÁ (Rhamdia quelen)

A considerable problem in the catfish larvae rearing is related to the first feeding. This study aimed to determine the larvae growth and the survival of silver catfish submitted to feeding with Artemia sp. in different time periods. The study was conducted in the Aquaculture Laboratory of the Universidade Estadual do Oeste do Paraná, with four treatments and five replications, constituted by feeding with Artemia sp. in 5, 10, 15 and 20 days, and after  each period of time, by its replacement by ration. We used 200 larvae distributed into 20 aquariums, soon after the eggs hatched. The larvae were fed 4 times a day until apparent satiation for 30 days. The Artemia sp. feeding for 15 days provided the best results on weight gain, final length and survival.KEYWORDS: Aquaculture; Iguaçu river; native fish; nutrition.

Uma das dificuldades na larvicultura do jundiá está relacionada à primeira alimentação. Este experimento teve como objetivo determinar o crescimento e a sobrevivência de larvas de jundiá submetidas à alimentação com Artemia sp. em diferentes períodos de tempo. O estudo foi realizado no Laboratório de Aquicultura da Universidade Estadual do Oeste do Paraná, com quatro tratamentos e cinco repetições, constituídos pela alimentação com Artemia sp em 5, 10, 15 e 20 dias, e após cada período, por sua substituição pela ração. Foram utilizadas 200 larvas distribuídas em 20 aquários, logo após a eclosão dos ovos. As larvas foram alimentadas 4 vezes ao dia até a saciedade aparente por um período de 30 dias. A alimentação com Artemia sp. durante 15 dias proporcionou os melhores resultados de ganho de peso, comprimento final e sobrevivência.PALAVRAS-CHAVE: Aquicultura; nutrição; peixe nativo; rio Iguaçu.

Alimentary handling in the larviculture of mandi-pintado (Pimelodus britskii) / Manejo alimentar na larvicultura do mandi-pintado ("Pimelodus britskii")

This study aimed to evaluate the growth rate and survival of mandi-pintado (Pimelodus britskii) larvae submitted to different feeding management. The experiment was conducted in a completely randomized design with four treatments and five replicates in a tank with 30 liters of water, each containing 30 larvae (0.032 ± 0.0048g and 8.22 ± 0.40mm). The treatments consisted of larvae fed with artemia; diet; artemia + diet; and artemia for two weeks + diet. Fish fed with artemia + diet showed greater weight and length, when compared to other feeding managements. The fish fed only with diet showed the worst performance. For the mandi-pintado larval rearing, the use of artemia combined with diet provides the best results for survival and growth.

O presente trabalho foi conduzido com o objetivo de avaliar a taxa de crescimento e sobrevivência de larvas de mandi-pintado (Pimelodus britskii) submetidas a diferentes manejos alimentares. O experimento foi realizado em um delineamento inteiramente casualizado com quatro tratamentos e cinco repetições, em aquários com 30 litros de água, cada um com 30 larvas (0,032 ± 0,0048g e 8,22 ± 0,40mm). Os tratamentos consistiram em larvas alimentadas com artemia; ração; artemia + ração e artemia por duas semanas + ração. Os peixes alimentados com artemia + ração apresentaram maior peso e comprimento final, quando comparados aos demais manejos alimentares. Os peixes alimentados apenas por ração apresentaram o pior desempenho. Para a larvicultura do mandi-pintado, a utilização de artemia combinada com ração proporciona os melhores resultados de sobrevivência e crescimento.

Alimentary handling in the larviculture of mandi-pintado (Pimelodus britskii) / Manejo alimentar na larvicultura do mandi-pintado ("Pimelodus britskii")

This study aimed to evaluate the growth rate and survival of mandi-pintado (Pimelodus britskii) larvae submitted to different feeding management. The experiment was conducted in a completely randomized design with four treatments and five replicates in a tank with 30 liters of water, each containing 30 larvae (0.032 ± 0.0048g and 8.22 ± 0.40mm). The treatments consisted of larvae fed with artemia; diet; artemia + diet; and artemia for two weeks + diet. Fish fed with artemia + diet showed greater weight and length, when compared to other feeding managements. The fish fed only with diet showed the worst performance. For the mandi-pintado larval rearing, the use of artemia combined with diet provides the best results for survival and growth.

O presente trabalho foi conduzido com o objetivo de avaliar a taxa de crescimento e sobrevivência de larvas de mandi-pintado (Pimelodus britskii) submetidas a diferentes manejos alimentares. O experimento foi realizado em um delineamento inteiramente casualizado com quatro tratamentos e cinco repetições, em aquários com 30 litros de água, cada um com 30 larvas (0,032 ± 0,0048g e 8,22 ± 0,40mm). Os tratamentos consistiram em larvas alimentadas com artemia; ração; artemia + ração e artemia por duas semanas + ração. Os peixes alimentados com artemia + ração apresentaram maior peso e comprimento final, quando comparados aos demais manejos alimentares. Os peixes alimentados apenas por ração apresentaram o pior desempenho. Para a larvicultura do mandi-pintado, a utilização de artemia combinada com ração proporciona os melhores resultados de sobrevivência e crescimento.