Cost evaluation of the use of conventional and electronic identification and registration systems for the national sheep and goat populations in Spain.

A cost model was developed to compare different implementation strategies of the new European Commission regulation for sheep and goat identification and registration (EC 21/2004) in Spain. Strategies were as follows: 1) conventional identification (CID) by two ear tags; 2) electronic identification (EID) by one bolus and one ear tag; and 3) mixed CID and EID strategy (MID), consisting of CID for fattening stock and EID for breeding stock. Complete and simplified implementations of the regulation were considered as options. Total costs per animal identified for all strategies and options varied according to the implementation option, ranging from Euros 2.48 and 4.64. The EID was the most expensive strategy (Euros 4.47 to 4.64) for all implementation options. Cost of CID and MID strategies ranged from Euros 2.63 to 2.98 and from Euros 2.48 to 3.03, respectively. The model was submitted to a sensitivity analysis without considering extra benefits of sheep and goat identification. Critical values for which the cost of MID equaled CID depended on strategy and option, and ranged from 7.5 to 11.5% for ear tag losses and from Euros 1.80 to 3.30 for bolus price. In conclusion, the use of a mixed strategy combining conventional ear tags (animals intended for slaughter) and electronic boluses (breeding stock) seems to be an affordable strategy that fulfills the European Commission regulation requirements for the identification of sheep and goats in Spain. Price reductions for devices and equipment would make the full electronic identification strategy less expensive in the future.

Regiocontrolled one-step synthesis of 3,3'-disubstituted 2,2'-bipyridine ligands by cobalt(I)-catalyzed cyclotrimerization.

A one-step, regioselective synthesis of annelated symmetric and asymmetric 3,3'-disubstituted 2,2'-bipyridines by cobalt(I)-catalyzed [2+2+2] cycloadditions between 5-hexynenitrile and 1,3-diynes is described. In the symmetric case, the total regioselectivity of the first cycloaddition is ensured electronically by the conjugation of the triple bonds, and for aminomethylated diynes that of the second is ensured by the cobalt coordinating to the aminomethyl rather than to the hexynenitrile nitrogen. In the asymmetric case, the first cycloaddition took place chemoselectively, which in the case of bis(trimethylsilyl)-1,3,5-hexatriyne (viewed as a 1,3-diyne) is explained by semiempirical calculation of LUMO coefficients. The copper(I) complex of 6b, constituting the first reported complex of the form ML2 (L is a symmetric 3,3'-disubstituted 2,2'-bipyridine), has been prepared. It had UV/Vis and NMR spectra reflecting the 3-substituent-induced mutual torsion of the bipyridine rings in the cis conformation, as was confirmed by x-ray diffractometric determination. The bipyridine 6c forms the dinuclear complex [Cu2(6c)2(CH3CN)2]2+ in the solid state.

Intramolecular

2-Propynyldiarylacetylenes undergo thermal intramolecular [4 + 2] cycloaddition to give benzo[b]fluorene derivatives in good yields. The hybridization of the tether connecting the reacting alkynes has a pronounced effect on the course of the reaction. Theoretical calculations and isotopic labeling studies support a mechanism which involves the generation of a cyclic allene intermediate that evolves to the final benzo[b]fluorene.