[Comparative characteristics of color reactions to ecdysteroids]. / Sravnitel'naia kharakteristika tsvetnykh reaktsii na ékdisteroidy.

The colour reactions used to determine sterols have been compared to find most sensitive method of quantitative determination of ecdysteroids. It is shown that the Liberman-Burchard reaction is most sensitive to most ecdysteroids, while the Chugaev reaction is most specific.

[Biochemical characterization of fractionated rat liver chromatin in experimental D-hypovitaminosis and after administration of steroidal drugs]. / Biokhimicheskie kharakteristiki fraktsionirovannogo khromatina pecheni krys v usloviiakh éksperimental'nogo D-gipovitaminoza i pri vvedenii preparatov steroidov.

Marked changes in the structural and functional characteristics of liver nuclear chromatin fractions are observed under experimental D-hypovitaminosis, which differ in the degree of transcriptional activity. DNA-polymerase activity and activity of the fraction, enriched with RNA-polymerase I, increases in the active fraction. Free radical LPO reactions are modified in the chromatin fraction with low activity and to the less degree in the active one. Disturbances of chromatine structural properties are caused with the change in the protein and lipid components of chromatin. Administration of ecdysterone preparations (separately and together with vitamin D3) has a partial corrective effect on structural and functional organization of nuclear chromatine. At the action of ecdysterone normalization of LPO reactions modified by pathological changes is observed in the chromatin fraction with low activity and to the less degree in the active one. This kind of influence corrects to the less degree chromatin functional activity and quantitative and qualitative modifications of its protein component. Simultaneous influence of ecdysterone and vitamin D3 leads to the partial normalization of the biochemical indices studied (except for those which characterize LPO reactions) mainly in the active chromatin fraction.

[Antiradical properties and antioxidant activity of ecdysterone]. / Antiradikal'nye svoistva i antiokislitel'naia aktivnost' ékdisterona.

Antiradical properties and total antioxidative activity of ecdysterone (10(-6)-10(-3)M were found on the model systems--photochemical one and that containing phosphatidylcholine liposomes oxidated with Fe2+ and Fe3+. The antioxidative activity of ecdysterone was shown to be comparable with that of the known inhibitors of peroxide oxidation of lipids--diethylparaphenylendiamine and ethylendiamine tetraacetate.