RESUMO
Testicular germ cell tumors account for about 1% of all cancers. The incidence of these tumors is increasing and they represent the most common solid malignancies of young men aged 15-40 years with seminoma being one of the most common histotype. Pathogenesis of testicular germ cell tumors remains unknown and, although cryptorchidism is considered the main risk factor, there is evidence of an association with environmental and genetic risk factors. Human papillomaviruses (HPV) are a family of DNA viruses and represent a major risk factor for cervical cancer. In addition, they have been associated with other human non-malignant and malignant diseases, including breast and head and neck cancer. HPV sequences have been detected throughout the male lower genitourinary tract as well as in seminal fluid and an increased testicular tumorigenesis has been reported in HPV transgenic mice. Aim of this study was to evaluate the potential involvement of HPV in human testicular tumorigenesis. Real-time PCR employing GP5+/GP6+ consensus HPV primers was used to examine the presence of HPV sequences in a subset of human seminoma (n = 61) and normal testicles (n = 23). None of the specimens tested displayed the presence of HPV DNA. These findings do not support an association between HPV and human seminoma and warrant further studies to assess definitively the role of these viruses in human testicular tumorigenesis.
Assuntos
Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/complicações , Infecções por Papillomavirus/virologia , Seminoma/etiologia , Seminoma/virologia , Animais , Primers do DNA/genética , Humanos , Masculino , Camundongos , Camundongos Transgênicos , Papillomaviridae/genética , Reação em Cadeia da Polimerase/métodos , Proteínas Estruturais Virais/genéticaRESUMO
We report an unusual case of documented Bartonella henselae genotype I from hepatic tissue in an Italian immunocompetent girl presenting with erythema nodosum and hepatic granulomata. Polymerase chain reaction (PCR) was performed on biopsied liver sample to confirm the etiologic role of B. henselae and to identify the genetic variant of this organism. A PCR on the same liver biopsy for parvovirus B19 was also positive, but the clinical meaning of this was not clear.
Assuntos
Infecções por Bartonella/diagnóstico , Bartonella henselae/genética , Eritema Nodoso/etiologia , Parvovirus B19 Humano/genética , Antibacterianos/uso terapêutico , Infecções por Bartonella/complicações , Infecções por Bartonella/tratamento farmacológico , Bartonella henselae/classificação , Pré-Escolar , Claritromicina/uso terapêutico , Eritema Nodoso/tratamento farmacológico , Feminino , Granuloma/tratamento farmacológico , Granuloma/microbiologia , Humanos , Imunocompetência , Hepatopatias/tratamento farmacológico , Hepatopatias/microbiologia , Infecções por Parvoviridae/complicações , Infecções por Parvoviridae/diagnóstico , Infecções por Parvoviridae/genética , Reação em Cadeia da PolimeraseRESUMO
A case of primary infection by human herpesvirus 6 (HHV-6) variant A in a 54-year-old woman, which occurred at the same time as the onset of encephalomyelitis, is reported. The correlation between the two events is discussed. It is speculated that, during the early phase of the infection, the HHV-6 spread to the central nervous system and triggered a pathogenic process that initially developed without symptoms. When the neurological disorders appeared, HHV-6 had already established a latent state: only the virus carried by infected blood cells was detected in the cerebrospinal fluid.
