RESUMO
The extracts of Desulfovibrio desulfuricans B-1388 cells, grown in anaerobic condition, display the superoxide dismutase activity. The maximum value of level activity (1.02 E/min/mg) is observed in the stationary phase of growth. Essentially the whole enzyme is localized in periplasmic fraction. Cells Desulfovibrio desulfuricans B-1388 do not show the catalase activity but contain active NADH- and NADPH-peroxidases. The activity of involved peroxidases depends on the physiological condition of culture.
RESUMO
Extracts of Desulfovibrio desulfuricans B-1388 cells grown under anaerobic conditions displayed superoxide dismutase activity. The maximal activity was found during the stationary growth phase. The enzyme was virtually completely located in the periplasm fraction. D. desulfuricans B-1388 lacked catalase activity but contained active NADH- and NADPH-peroxidases. The activity of NADH-peroxidase depended on the physiological state of the culture. On changing the growth conditions (the presence of 5% CO in the gaseous phase), the activity of superoxide dismutase decreased.