RESUMO
Twenty-one operable breast cancer patients were treated with interferon-beta (IFN-beta) at the dosage of 3 x 10(6) IU every other day for a median time of 16 days. Estrogen receptors (ER), progesterone receptors (PgR), labelling index (LI) and DNA ploidy were evaluated on tumour tissue obtained during diagnostic biopsy and surgery, before and after treatment respectively. The study showed a statistically significant rise of ER (18 out of 21 evaluable cases) (p = 0.04) and no influence on ploidy status (8/21) in all evaluable samples. We also noticed a rise of PgR (18/21) and a decrease of LI (10/21 cases), but they were not statistically significant. Moreover, in the patients in whom both determination of ER and LI was possible (10 cases), a significant inverse correlation between the differences pre- and post-treatment was observed (p = 0.016). These preliminary results induce us to study a greater number of patients with the aim to confirm the biological effects of IFN-beta on ER, to clarify the trend of the rise of PgR and the decrease of LI and to analyse the relationship between these parameters.
Assuntos
Neoplasias da Mama/terapia , DNA de Neoplasias/análise , Fatores Imunológicos/uso terapêutico , Interferon beta/uso terapêutico , Proteínas de Neoplasias/análise , Receptores de Estrogênio/análise , Receptores de Progesterona/análise , Adulto , Idoso , Aneuploidia , Neoplasias da Mama/química , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Neoplasias da Mama/cirurgia , Terapia Combinada , Feminino , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Fatores Imunológicos/farmacologia , Interferon beta/farmacologia , Pessoa de Meia-Idade , Índice Mitótico/efeitos dos fármacos , Proteínas de Neoplasias/genética , Neoplasias Hormônio-Dependentes/química , Neoplasias Hormônio-Dependentes/genética , Neoplasias Hormônio-Dependentes/patologia , Neoplasias Hormônio-Dependentes/cirurgia , Neoplasias Hormônio-Dependentes/terapia , Receptores de Estrogênio/genética , Receptores de Progesterona/genéticaRESUMO
In this paper are reported the results obtained by a simple, reproducible and sensitive rT3 RIA, in unextracted serum, using the PEG separation technique. The linear correlation coefficient of the results obtained from extracted and unextracted sera, in a population of 60 adults and 35 child controls was 0.984, with a minimal spread of the data.
