[The dynamics of hormonal changes in female rats exposed to chronic stress and androgen excess during pubescence].

The dynamics of blood plasma corticosterone, testosterone and androstenedione levels and their reaction to acute stress (30 min immobilization) in 35-, 40- and 45-day old female rats exposed to chronic stress (daily 30-min immobilization from 35th to 45th day of life) and/or to excess of exogenous androgens (implantation of capsules with testosterone to 33-day old animals) during pubescence was studied. Both control and experimental females in all age groups responded to acute stress by significant elevation of blood plasma corticosterone levels. At the end of the chronic stress session, the extent of adrenals activation in response to acute dosed stress was lowered in androgenized 45-day old females and increased gradually in stressed ones. After acute stress, the blood plasma testosterone level decreased in control 35-day old females and rose - in androgenized females against 10-fold rising of basal hormonal level. In 40-day old control females as well as in androgenized ones exposed to chronic stress during 5 days, the acute dosed stress did not result in significant changes of blood plasma testosterone and elevated blood plasma androstenedione. Stressed 40-day old females with increased basal androstenedione secretion did not respond to acute stress by the hormone level changes while blood plasma testosterone declined significantly. At the end ofpubescence (on the 45th day of life), acute stress did not affect the blood plasma testosterone level in control and androgenized animals, while decreased it in stressed females and increased - in androgenized rats exposed to chronic stress against elevated basal level of the hormone. The conclusion is made about possible functional relationship between the changes in hormonal homeostasis during pubescence and development of reproductive system in mature animals.

[Effects of cytokine-like polypeptide EMAP II and flutamide on the testosterone-stimulated prostate of castrated rats].

The effects of separate and combined administration of cytokine-like polypeptide EMAP II and flutamide, a nonsteroid antiandrogen, on morphology and function of the accessory sexual glands in castrated rats stimulated with testosterone propionate were studied. We found antiangiogenic, procoagulating and proapoptotic effects of EMAP II in the ventral prostate. Combined administration of the preparations enhanced their antiprostatic effects, which were manifested in inhibition of the androgen-dependent processes in prostate tissues, changes in proliferation and apoptosis, DNA, RNA and protein contents. We conclude that combined administration of EMAP II and flutamide can be used for development of new therapeutic modalities in prostate cancer.

[Effect of chronic stress during puberty on the reproductive system of female rats with experimental hyperandrogenicity].

Effects of chronic stress (daily 30-min immobilization) on 35-45 days of life and its combination with androgenization (implantation of testosterone-containing capsules on 33rd day of life) on reproductive system of 2.5 month old female rats were studied. The term of sexual maturation, estrous cycles regularity and structure, blood plasma levels of testosterone, progesterone and androstenedione as well as ovarian histology were examined. Androgenization resulted in the blood plasma testosterone level increase and the androstenedione level decrease, development of oligo- or anovulatory condition characterized by disorders or discontinuation in estrous cyclicity. We also detected abrupt reduction or absence of postovulatory luteal bodies, cysts formation and ovarian interstitial tissue overgrowth. All experimental animals had normal blood plasma corticosterone level. Stressed rats had no considerable changes in reproductive system except of some cyclicity disorders. Stressed against androgenization rats demonstrated delayed pubescence, an increased number of ovarian cysts along with attenuation ofandrogenization-caused negative effects on the sexual cyclicity.

[Effect of combined application of the synthetic luteinizing hormone-releasing hormone agonist and nonsteroidal antiandrogen on the reproductive system of male rats].

The influence of combined use of different doses of LGRH agonist (Surfagon) and non-steroidal antiandrogen Flutamide (Niftolid) on the structure and functional state of accessory sexual glands and hypophyseal-gonadal system of rate males had been studied. Optimal ratio of the drug doses for achieving the best antiprostatic effect has been determined. It was shown that combined use of above mentioned drugs resulted in the potentiation of antiprostatic effects. A maximal effect was observed after 30 days of Surfagon administration in a dose of 50 mg/kg b.w. together with flutamid in a dose of 10 mg/kg. b.w. Such a combination of drugs is recommended for clinical approbation as therapy for prostate cancer.

[Nucleic acid and protein content in rat prostate during combined treatment with the gonadorelin agonist and an androgen receptor inhibitor]. / Vmist nukleïnovykh kyslot i bilka u peredmikhurovii zalozi shchuriv za umov poiednanoho zastosuvannia ahonista honadoliberynu ta blokatopa retseptoriv androheniv.

The effects of androgen receptor blocker Flutamide (Niftolide) and/or LH-releasing hormone agonist Surfagon on the nucleic acids and protein contents in the ventral prostate of male rats were studied. It was shown that combined drug treatment led to potentiating of "antiprostatic" effects of both preparations resulted in significant decrease of DNA, RNA and protein contents.