RESUMO
PURPOSE: To determine whether the Factor V (1691G/A), Factor V HR2 (4070A/G), Prothrombin (20210G/A), PAI-1 (-675 I/D, 5G/4G), ACE (intron 16 I/D), Factor VII (Gln353Arg), Factor XIII (Val34Leu), ß-fibrinogen (-455G/A), Glycoprotein Ia (807C/T), tPA (intron 8 D/I) gene mutations could be risk factors for recurrent pregnancy loss (RPL). METHODS: Genotyping of thrombophilic gene mutations were carried out by amplification Refractory Mutation System-PCR (ARMS-PCR) method after DNA extraction. RESULTS: We found that the mutant allele frequencies of Factor V (1691G/A), Factor V HR2 (4070A/G), Prothrombin (20210G/A), PAI-1 (-675 I/D, 5G/4G), Factor XIII (Val34Leu) and ß-fibrinogen (-455G/A) were more seen in the case group compared with the healthy control; However, the difference between the two group is not statistically significant (p > 0.05). Whilst the mutant allele frequencies of other studied genes were lower in the case in comparison to the fertile control women (p > 0.05). CONCLUSION: Taken together, our data has shown that the prevalence of thrombophilic gene mutations was similar in women with RPL and healthy controls. Therefore, it appears that further studies on large-scale population and other genetic variants will be needed to conclusively find candidate genes for RPL unknown etiology in the future.
Assuntos
Aborto Habitual/genética , Trombofilia/genética , Adulto , Fator V/genética , Fator VII/genética , Fator VIII/genética , Feminino , Fibrinogênio/genética , Frequência do Gene , Predisposição Genética para Doença , Genótipo , Humanos , Integrina alfa2/genética , Mutação , Peptidil Dipeptidase A/genética , Inibidor 1 de Ativador de Plasminogênio/genética , Gravidez , Protrombina/genética , Fatores de Risco , Adulto JovemRESUMO
Stressful events during gestation have important effects on the later physical and mental health of the offspring. In the study described here, the pilocarpine-induced seizure model was used to test the hypothesis that prenatal stress affects seizure susceptibility in infant rats. Prenatal stress consisted of daily restraint of the dam under normal room conditions (for 120minutes, twice daily) during the first, second, and third weeks of gestation. The pups were then compared with pups born to unstressed dams. Both second- and third-week-gestation stress significantly reduced pilocarpine-induced seizures in 19-day-old rat offspring, as compared with nonstressed control offspring. Mid- and late-gestation stress increased the rate and time of tonic-clonic seizures. Mortality rate 2 and 24hours after pilocarpine administration increased significantly in all stressed rats. Stress induced a significant rise in circulating corticosterone levels (2- to 8-fold, P<0.001) in the offspring. Female offspring differed little from male offspring with respect to blood corticosterone levels and epileptic behaviors. These findings indicate that prenatal stress, particularly during the second and third weeks of pregnancy, may play an important role in increasing seizure vulnerability in the unborn offspring. Female rats are more resistant to stress than males probably because of the lower susceptibility of their hypothalamic-pituitary-adrenal axis.
