Effect of surface treatment and resin cement type on the bond strength of polyetheretherketone to lithium disilicate ceramic.

BACKGROUND: This study aims to evaluate the effect of surface treatment and resin cement on the shear bond strength (SBS) and mode of failure of polyetheretherketone (PEEK) to lithium disilicate ceramic (LDC). This is suggested to study alternative veneering of PEEK frameworks with a ceramic material. METHODS: eighty discs were prepared from PEEK blank and from lithium disilicate ceramic. Samples were divided into four groups according to surface treatment: Group (A) air abraded with 110 µm Al2O3, Group (AP) air abrasion and primer application, Group (S) 98% sulfuric acid etching for 60 s, Group (SP) Sulfuric acid and primer. Each group was subdivided into two subgroups based on resin cement type used for bonding LDC:1) subgroup (L) self- adhesive resin cement and 2) subgroup (B) conventional resin cement (n = 10). Thermocycling was done for all samples. The bond strength was assessed using the shear bond strength test (SBS). Failure mode analysis was done at 50X magnification with a stereomicroscope. Samples were chosen from each group for scanning electron microscope (SEM). The three-way nested ANOVA followed by Tukey's post hoc test were used for statistical analysis of results. Comparisons of effects were done utilizing one way ANOVA and (p < 0.05). RESULTS: The highest mean of shear bond strength values was demonstrated in Group of air abrasion with primer application using conventional resin cement (APB) (12.21 ± 2.14 MPa). Sulfuric acid groups showed lower shear bond strength values and the majority failed in thermocycling especially when no primer was applied. The failure mode analysis showed that the predominant failure type was adhesive failure between cement and PEEK, while the remaining was mixed failure between cement and PEEK. CONCLUSION: The air abrasion followed by primer application and conventional resin cement used for bonding Lithium Disilicate to PEEK achieved the best bond strength. Primer application did not have an effect when self-adhesive resin cement was used in air-abraded groups. Priming step is mandatory whenever sulfuric acid etching surface treatment is utilized for PEEK.

Biaxial flexural strength of different provisional restorative materials under chemo-mechanical aging: An in vitro study.

PURPOSE: Durability is a critical factor for the success of long-term provisional restorations. This study evaluated the effect of different storage media and cyclic loading on the biaxial flexural strength of different types of provisional materials. MATERIALS AND METHODS: Discs (N = 360, 10 × 2 mm) were prepared from different provisional materials; conventional (Protemp 4, Tuff-Temp, Tempron), CAD-CAM milled (VITA CAD-Temp, breCAM.multiCOM), and 3D-printed (Nextdent C&B MFH) (n = 60). Each material group was subdivided into four subgroups according to the storage media (n = 15): nonaged, artificial saliva, mouthwash, and coffee. The specimens in storage media were placed in an incubator at 37°C for 4 weeks followed by 60,000 simulated chewing cycles. Biaxial flexural strength test was done. Data were analyzed using two-way ANOVA. Weibull distribution parameters were estimated. RESULTS: Both the material and aging protocol showed a significant effect on the biaxial flexural strength. Both Protemp 4 and Nextdent C&B MFH showed a significantly higher biaxial flexural strength before and after aging compared to all other materials. Protemp 4 and Tuff Temp showed a significant decrease in strength with aging. CAD-CAM materials showed higher Weibull moduli. CONCLUSIONS: The 3D-printed polymethylmethacrylate provisional material presents with both greater biaxial flexural strength and increased durability against chemical and mechanical aging compared to conventional and CAD-CAM milled provisional materials tested.

Effect of different intraoral scanners and post-space depths on the trueness of digital impressions.

BACKGROUND: The trueness of intraoral scanners (IOSs) has been evaluated in many clinical situations. However, the tests of their performance when scanning post-space preparations are still lacking. OBJECTIVES: The aim of the present study was to compare the trueness of the digital impressions of post spaces with different depths, captured by means of different IOSs. MATERIAL AND METHODS: Digital impressions of teeth (N = 16) with post spaces of depths of 8 mm and 10 mm were captured. Three IOSs were used, including Primescan AC, Medit i500 and CS 3600. The STL files were compared to the files obtained from the traditional impression scanning performed with an InEos X5 desktop scanner. Then, reverse engineering software measured the trueness values, which were analyzed using the two-way analysis of variance (ANOVA), followed by Tukey's post-hoc test. The significance level was set at p < 0.05. RESULTS: Significant differences were found between the scanners in terms of root mean square (RMS) values (p < 0.001). The highest RMS value was found for CS 3600 (0.30 ±0.11 mm), followed by Primescan AC (0.26 ±0.09 mm), while the lowest value was found for Medit i500 (0.18 ±0.05 mm). The 8-millimeter-deep post spaces had a significantly higher RMS value than the 10-millimeter-deep ones (0.28 ±0.10 mm and 0.21 ±0.09 mm, respectively) (p = 0.009). CONCLUSIONS: The Medit i500 scanner showed the highest post-space digital impression trueness as compared to Primescan AC and CS 3600. In the digital impressions captured with CS 3600, the 10 mm postspace depth had higher trueness than the 8 mm depth. Moreover, CS 3600 was less able to capture the full length of both the 8 mm and 10 mm post-space depths than Primescan AC and Medit i500.

The effect of Er, Cr:YSGG laser debonding on the bond strength of two ceramic materials to dentin.

OBJECTIVES: Evaluating the bond strength of two ceramic materials to dentin after Er,Cr:YSGG laser debonding. Would laser debonding affect the bond strength of ceramic to dentin? MATERIALS AND METHODS: Recently extracted human molars were ground to expose dentin. Forty square shaped samples were prepared from CAD/CAM ceramic blocks. Samples were divided into two groups according to the type of ceramic material; group E: Lithium disilicate and group T: Ultra-translucent Zirconia (n = 20) Each group was divided into two subgroups (n = 10) according to the laser debonding effect (subgroup B: bonded samples, subgroup R: re-bonded samples after laser debonding). Ceramic samples were bonded to dentin using dual cure self-adhesive resin cement. Laser debonding of ceramic samples of subgroups R using Er, Cr:YSGG laser, were then re-bonded again to dentin surface with same resin cement. The Shear bond strength test using Universal testing machine was done. The failure mode was analyzed. Two-way analysis of variance was used to compare the mean bond strength and re-bond strength of two materials. The significance level was set at P ≤ 0.05. RESULTS: Two-Way ANOVA showed that ceramic type had a significant effect on the re-bond strength to dentin. The predominant failure mode was adhesive. CONCLUSIONS: Laser debonding of Lithium disilicate and Ultra translucent Zirconia decreased the re-bond strength to dentin. Deterioration in re-bond strength for Lithium disilicate ceramics was more pronounced than for Ultra translucent Zirconia. Clinical Relevance Deterioration in the bond strength between ceramics & dentin after laser debonding still needs improvement to allow its clinical use.

The Development of FVIII Inhibitors in Relation to IL10 Gene Polymorphism in Hemophilia A Egyptian Pediatric Patients.

BACKGROUND: Development of inhibitors against Factor VIII (FVIII) in hemophilia A patients is a serious complication of therapy. Many cytokines, including interleukin-10 (IL10), may affect inhibitor development; however, literature data are not sufficient to prove this association. The aim of this study was to investigate the relation between FVIII inhibitor formation and IL10-1082A/G polymorphism among Egyptian hemophiliacs. METHODS: Patients were screened for FVIII inhibitors using the Bethesda method. IL10-1082A/G polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism. RESULTS: Six patients (12%) developed inhibitors. No statistically significant difference was found between inhibitor positive and negative patients regarding IL10-1082A/G genotypes, disease severity, or treatment-related variables (type of FVIII received, treatment regimen, age at first exposure to FVIII, and frequency of replacement therapy). CONCLUSIONS: FVIII inhibitor formation in this group of Egyptian hemophiliacs was not correlated to IL10-1082A/G polymorphism, disease severity, or any of the treatment variables.

Quantitative assessment of Wilms tumor 1 (WT1) gene transcripts in Egyptian acute lymphoblastic leukemia patients.

BACKGROUND: Accurate assessment of minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL) patients after initial chemotherapy is essential to evaluate the efficacy of therapeutic regimens. Wilms tumor 1 (WT1) is a pan-leukemic marker used for identification of the leukemic clone rather than the use of individual specific molecular aberration of ALL. METHODS: Using a real-time quantitative polymerase chain reaction, bone marrow samples from 41 newly diagnosed Egyptian ALL patients; 22 adults and 19 children were examined for WT1 expression. After induction therapy, WT1 expression was reestimated in 20 ALL patients. RESULTS: WT1 was overexpressed in adult and pediatric ALL patients (95.4% and 89.4%, respectively). WT1 expression at diagnosis had no statistically significant impact on disease-free survival of patients (P = 0.054). However, WT1 expression increased after induction chemotherapy in the 3 pediatric patients who had relapse. CONCLUSIONS: WT1 is a leukemia-associated molecular marker that may be used for the diagnosis and for monitoring clinical progress in ALL; it also can be used as a molecular target for adoptive immunotherapy.