RESUMO
Streptococcus suis, a zoonotic bacterial pathogen circulated through swine, can cause severe infections in humans. Because human S. suis infections are not notifiable in most countries, incidence is underestimated. We aimed to increase insight into the molecular epidemiology of human S. suis infections in Europe. To procure data, we surveyed 7 reference laboratories and performed a systematic review of the scientific literature. We identified 236 cases of human S. suis infection from those sources and an additional 87 by scanning gray literature. We performed whole-genome sequencing to type 46 zoonotic S. suis isolates and combined them with 28 publicly available genomes in a core-genome phylogeny. Clonal complex (CC) 1 isolates accounted for 87% of typed human infections; CC20, CC25, CC87, and CC94 also caused infections. Emergence of diverse zoonotic clades and notable severity of illness in humans support classifying S. suis infection as a notifiable condition.
Assuntos
Streptococcus suis , Humanos , Animais , Suínos , Epidemiologia Molecular , Streptococcus suis/genética , Europa (Continente)/epidemiologia , Filogenia , Sequenciamento Completo do GenomaRESUMO
Strain HF14-78462T is an environmental bacterium found in clinical samples from an immunocompromized patient in 2014 at Hospital Universitari i Politècnic La Fe (Valencia, Spain). Phenotypically, strain HF14-78462T cells were Gram-stain-negative, aerobic, non-spore forming and non-motile small rods which formed mucous and whitish-translucent colonies when incubated at 20-36 °C. Phylogenetic analyses based on the 16S rRNA genes and the whole genomes of closest sequenced relatives confirmed that strain HF14-78462T is affiliated with the genus Starkeya. The strain was oxidase, catalase and urease positive; but indole, lysine decarboxylase, ornithine decarboxylase and DNase negative, did not produce H2S and was able to utilize a wide variety of carbon sources including acetamide, adonitol, amygdalin, l-arabinose, citric acid, glucose, mannitol and melibiose. Unlike Starkeya novella and Starkeya koreensis, strain HF14-78462T failed to grow in thiosulphate-oxidizing media and had a narrower temperature growth range. Its genome was characterized by a size of 4.83 Mbp and a C+G content of 67.75âmol%. Major fatty acids were C18:1 ω7c, cyclo C19â:â0 and C16â:â0, its polar acids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an aminophospholipid; while the ubiquinones were Q9 (1.8â%) and Q10 (98.2â%). Digital DNA-DNA hybridization values were 41 and 41.4 against S. novella and S. koreensis, respectively, while average nucleotide identity values were around 84â%. Phenotypic, average nucleotide identity and phylogenomic comparative studies suggest that strain HF14-78462T is a new representative of the genus Starkeya and the name Starkeya nomas sp. nov. is proposed. The type strain is HF14-78462T (=CECT 30124T=LMG 31874T).
Assuntos
Ácidos Graxos , Noma , Humanos , Ácidos Graxos/química , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , DNA Bacteriano/genética , Técnicas de Tipagem Bacteriana , Composição de Bases , BactériasRESUMO
No disponible
Assuntos
Humanos , Masculino , Adulto , Bacteriemia/diagnóstico , Bacteriemia/etiologia , Cupriavidus/isolamento & purificação , Colite Ulcerativa/complicações , Bacteriemia/microbiologia , Imunossupressores/uso terapêutico , Bacilos e Cocos Aeróbios Gram-Negativos/isolamento & purificação , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Imipenem/uso terapêuticoAssuntos
Antibacterianos/farmacologia , Bacteriemia/diagnóstico , Chryseobacterium/isolamento & purificação , Colistina/farmacologia , Farmacorresistência Bacteriana , Infecções por Bactérias Gram-Negativas/diagnóstico , Antibacterianos/administração & dosagem , Transtorno Autístico/complicações , Bacteriemia/microbiologia , Bacteriemia/patologia , Criança , Chryseobacterium/efeitos dos fármacos , Ciprofloxacina/administração & dosagem , Feminino , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/patologia , Humanos , Resultado do Tratamento , Combinação Trimetoprima e Sulfametoxazol/administração & dosagemRESUMO
Streptococcus pyogenes appears to be almost exclusively restricted to humans, with few reports on isolation from animals. We provide a detailed characterization (emm typing, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing [MLST]) of 15 S. pyogenes isolates from animals associated with different clinical backgrounds. We also investigated erythromycin resistance mechanisms and phenotypes and virulence genes. We observed 2 emm types: emm12 (11 isolates) and emm77 (4 isolates). Similarly, we observed 2 genetic linages, sequence type (ST) 26 and ST63. Most isolates exhibited the M macrolide resistance phenotype and the mefA/ermB genotype. Isolates were grouped into 2 clones on the basis of emm-MLST-PFGE-virulence gene profile combinations: clone 1, characterized by the combined genotype emm12-ST36-pulsotype A-speG; and clone 2, characterized by the genotype emm77-ST63-pulsotype B-speC. Our results do not show conclusively that animals may represent a new reservoir of S. pyogenes but indicate the ability of human-derived S. pyogenes isolates to colonize and infect animals.
Assuntos
Farmacorresistência Bacteriana/genética , Genótipo , Doenças dos Ovinos/epidemiologia , Infecções Estreptocócicas/epidemiologia , Infecções Estreptocócicas/veterinária , Streptococcus pyogenes/genética , Animais , Antibacterianos/farmacologia , Antígenos de Bactérias/genética , Antígenos de Bactérias/metabolismo , Proteínas da Membrana Bacteriana Externa/genética , Proteínas da Membrana Bacteriana Externa/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Células Clonais , Eletroforese em Gel de Campo Pulsado , Fazendas , Expressão Gênica , Macrolídeos/farmacologia , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Testes de Sensibilidade Microbiana , Tipagem de Sequências Multilocus , Fenótipo , Coelhos , Ovinos/microbiologia , Doenças dos Ovinos/tratamento farmacológico , Doenças dos Ovinos/microbiologia , Doenças dos Ovinos/transmissão , Espanha , Infecções Estreptocócicas/tratamento farmacológico , Infecções Estreptocócicas/transmissão , Streptococcus pyogenes/classificação , Streptococcus pyogenes/efeitos dos fármacos , Streptococcus pyogenes/isolamento & purificaçãoRESUMO
BACKGROUND: Members of the genus Pseudonocardia have been widely reported and recovered from several ecosystems, such as soil samples and plant samples. Pseudonocardia bacteria colonize the microbial communities on the integument of fungus gardening ant species. We present the first documented case of Pseudonocardia carboxydivorans isolated in human cerebrospinal fluid (CSF). To the best of our knowledge, this is the first report of an human infection by P. carboxydivorans. CASE PRESENTATION: A patient, who suffered a traumatic brain injury a month before, was admitted to this hospital due to gait alteration and cognitive disturbances. Culture of cerebrospinal fluid showed ramified, not acid-fast, Gram positive bacilli. The bacterium was identified by molecular methods as P. carboxydivorans. CONCLUSION: This is the first documented case of isolating P. carboxydivorans in human CSF in a case of probable meningitis. Further research is needed in order to determine its pathogenic role in human infections.
Assuntos
Actinobacteria/isolamento & purificação , Lesões Encefálicas Traumáticas/complicações , Infecções por Bactérias Gram-Positivas/líquido cefalorraquidiano , Actinobacteria/genética , Actinobacteria/patogenicidade , Idoso , Lesões Encefálicas Traumáticas/microbiologia , Líquido Cefalorraquidiano/microbiologia , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Infecções por Bactérias Gram-Positivas/etiologia , Infecções por Bactérias Gram-Positivas/microbiologia , Humanos , MasculinoRESUMO
No disponible
Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Peritonite/complicações , Peritonite/diagnóstico , Peritonite/tratamento farmacológico , Streptococcus equi , Streptococcus equi/isolamento & purificação , Paracentese/métodos , Aztreonam/uso terapêutico , Metronidazol/uso terapêutico , Testes de Sensibilidade Microbiana/métodos , Levofloxacino/uso terapêutico , Colangiografia , Constrição Patológica/complicações , Constrição PatológicaRESUMO
Christensenella minuta is a Gram-negative strictly anaerobic short rod that inhabits the human gut. This bacterium was isolated in a mixed infection with Desulfovibrio desulfuricansfrom the blood of a patient with a diagnosis of acute appendicitis. The strain was identified by 16S rRNA sequence analysis. As far as we know, this is the first time C.minuta has been isolated from a human clinical specimen.
Assuntos
Apendicite/diagnóstico , Bacteriemia/diagnóstico , Clostridiales/isolamento & purificação , Coinfecção/diagnóstico , Infecções por Bactérias Gram-Positivas/diagnóstico , Adolescente , Apendicite/complicações , Apendicite/microbiologia , Bacteriemia/microbiologia , Bacteriemia/patologia , Clostridiales/classificação , Clostridiales/genética , Coinfecção/microbiologia , Coinfecção/patologia , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Desulfovibrio desulfuricans/classificação , Desulfovibrio desulfuricans/genética , Desulfovibrio desulfuricans/isolamento & purificação , Infecções por Desulfovibrionaceae/diagnóstico , Infecções por Desulfovibrionaceae/microbiologia , Infecções por Desulfovibrionaceae/patologia , Infecções por Bactérias Gram-Positivas/microbiologia , Infecções por Bactérias Gram-Positivas/patologia , Humanos , Masculino , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
Streptococcus dysgalactiae subsp. equisimilis (SDSE) has virulence factors similar to those of Streptococcus pyogenes. Therefore, it causes pharyngitis and severe infections indistinguishable from those caused by the classic pathogen. The objectives of this study were: to know the prevalence of SDSE invasive infections in Argentina, to study the genetic diversity, to determine the presence of virulence genes, to study antibiotic susceptibility and to detect antibiotic resistance genes. Conventional methods of identification were used. Antibiotic susceptibility was determined by the disk diffusion and the agar dilution methods and the E-test. Twenty eight centers from 16 Argentinean cities participated in the study. Twenty three isolates (16 group G and 7 group C) were obtained between July 1 2011 and June 30 2012. Two adult patients died (8.7%). Most of the isolates were recovered from blood (60.9%). All isolates carried speJ and ssa genes. stG62647, stG653 and stG840 were the most frequent emm types. Nineteen different PFGE patterns were detected. All isolates were susceptible to penicillin and levofloxacin, 6 (26.1%) showed resistance or reduced susceptibility to erythromycin --#91;1 mef(A), 3 erm(TR), 1 mef(A) + erm(TR) and 1 erm(TR) + erm(B)--#93; and 7 (30.4%) were resistant or exhibited reduced susceptibility to tetracycline --#91;2 tet(M), 5 tet(M) + tet(O)--#93;. The prevalence in Argentina was of at least 23 invasive infections by SDSE. A wide genetic diversity was observed. All isolates carried speJ and ssa genes. Similarly to other studies, macrolide resistance (26.1%) was mainly associated to the MLS B phenotype.
Streptococcus dysgalactiae subsp. equisimilis (SDSE) posee factores de virulencia similares a Streptococcus pyogenes y, en consecuencia, produce faringitis e infecciones graves indistinguibles de las generadas por este patógeno clásico. Los objetivos del estudio fueron conocer la prevalencia de SDSE en infecciones invasivas en Argentina, estudiar su diversidad genética, determinar la presencia de genes de virulencia, ensayar su sensibilidad a los antibióticos y conocer los genes de resistencia. Se emplearon métodos convencionales de identificación. La sensibilidad se determinó por difusión, Etest y dilución en agar. Participaron 28 centros de 16 ciudades argentinas. Se obtuvieron 23 aislamientos (16 del grupo G y 7 del grupo C) desde el 1-7-2011 hasta el 30-6-2012. Se registraron 2 muertes en adultos (8,7%). La mayoría de los aislamientos fueron obtenidos de sangre (60,9%). Todos eran portadores de los genes speJ y ssa. Los genotipos más frecuentes fueron stG62647, stG653 y stG840. Se detectaron 19 pulsotipos distintos. Todos los aislamientos fueron sensibles a penicilina y levofloxacina, 6 (26,1%) presentaron resistencia o sensibilidad disminuida a eritromicina (1 mef--#91;A--#93;, 3 erm--#91;TR--#93;, 1 mef--#91;A--#93; + erm--#91;TR--#93; y 1 erm--#91;TR--#93; + erm--#91;B--#93;) y 7 (30,4%) fueron resistentes o tuvieron sensibilidad disminuida a tetraciclina (2 tet--#91;M--#93;, 5 tet--#91;M--#93; + tet--#91;O--#93;). La prevalencia anual en la Argentina fue de al menos 23 infecciones invasivas por SDSE y se observó una amplia diversidad genética. Todos los aislamientos presentaron los genes ssa y speJ. Como en otros estudios, la resistencia a macrólidos (26,1%) estuvo asociada, principalmente, al fenotipo MLS B.
Assuntos
Humanos , Masculino , Feminino , Infecções Estreptocócicas/classificação , Streptococcus/isolamento & purificação , Streptococcus/patogenicidade , Argentina , Streptococcus/genética , Resistência Microbiana a Medicamentos , Estudos Transversais/métodosRESUMO
No disponible
Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Bordetella/isolamento & purificação , Infecções por Bordetella/complicações , Infecções por Bordetella/tratamento farmacológico , Infecções por Bordetella/patologia , Testes de Sensibilidade Microbiana/métodos , Testes de Sensibilidade Microbiana , Fibrose Cística/complicações , 51426 , Combinação Trimetoprima e Sulfametoxazol/uso terapêutico , Penicilinas/uso terapêutico , Cefalosporinas/uso terapêutico , Minociclina/uso terapêuticoRESUMO
No disponible
Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Bactéria Gordonia , Bactéria Gordonia/isolamento & purificação , Abscesso/complicações , Abscesso/diagnóstico , Abscesso/tratamento farmacológico , Combinação Amoxicilina e Clavulanato de Potássio/uso terapêutico , Ágar/análise , Anti-Infecciosos/uso terapêutico , Escarro/citologia , Escarro/microbiologiaRESUMO
Human infections caused by Streptococcus lactarius have not been previously reported. In the present report, we describe a lactational mastitis caused by this organism. The infection occurred in a 28-year-old breast-feeding female, with a 10-days history of moderate pain on the right breast. The patient was cured after antibiotic treatment with levofloxacin for 21 days. Our case shows that S. lactarius should be considered as a cause of lactational mastitis. The introduction of molecular microbiology techniques can be extremely useful for knowing the implication of streptococci in lactational mastitis.
Assuntos
Mastite/etiologia , Mastite/patologia , Infecções Estreptocócicas/diagnóstico , Infecções Estreptocócicas/patologia , Streptococcus/classificação , Streptococcus/isolamento & purificação , Adulto , Antibacterianos/uso terapêutico , Feminino , Humanos , Levofloxacino/uso terapêutico , Mastite/tratamento farmacológico , Mastite/microbiologia , Técnicas de Diagnóstico Molecular , Infecções Estreptocócicas/tratamento farmacológico , Infecções Estreptocócicas/microbiologia , Resultado do TratamentoRESUMO
No disponible
Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Nocardia/isolamento & purificação , Nocardiose/microbiologia , Injeções Intralesionais/efeitos adversos , Osteoartrite/tratamento farmacológicoRESUMO
Streptococcus dysgalactiae subsp. equisimilis (SDSE) has virulence factors similar to those of Streptococcus pyogenes. Therefore, it causes pharyngitis and severe infections indistinguishable from those caused by the classic pathogen. The objectives of this study were: to know the prevalence of SDSE invasive infections in Argentina, to study the genetic diversity, to determine the presence of virulence genes, to study antibiotic susceptibility and to detect antibiotic resistance genes. Conventional methods of identification were used. Antibiotic susceptibility was determined by the disk diffusion and the agar dilution methods and the E-test. Twenty eight centers from 16 Argentinean cities participated in the study. Twenty three isolates (16 group G and 7 group C) were obtained between July 1 2011 and June 30 2012. Two adult patients died (8.7%). Most of the isolates were recovered from blood (60.9%). All isolates carried speJ and ssa genes. stG62647, stG653 and stG840 were the most frequent emm types. Nineteen different PFGE patterns were detected. All isolates were susceptible to penicillin and levofloxacin, 6 (26.1%) showed resistance or reduced susceptibility to erythromycin [1 mef(A), 3 erm(TR), 1 mef(A)+erm(TR) and 1 erm(TR)+erm(B)] and 7 (30.4%) were resistant or exhibited reduced susceptibility to tetracycline [2 tet(M), 5 tet(M)+tet(O)]. The prevalence in Argentina was of at least 23 invasive infections by SDSE. A wide genetic diversity was observed. All isolates carried speJ and ssa genes. Similarly to other studies, macrolide resistance (26.1%) was mainly associated to the MLSB phenotype.
Assuntos
Bacteriemia/microbiologia , Infecções Estreptocócicas/microbiologia , Streptococcus/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Argentina/epidemiologia , Artrite Infecciosa/epidemiologia , Artrite Infecciosa/microbiologia , Bacteriemia/epidemiologia , Líquidos Corporais/microbiologia , Criança , Pré-Escolar , DNA Bacteriano/genética , Farmacorresistência Bacteriana Múltipla/genética , Eletroforese em Gel de Campo Pulsado , Feminino , Genes Bacterianos , Variação Genética , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Prevalência , Infecções dos Tecidos Moles/epidemiologia , Infecções dos Tecidos Moles/microbiologia , Infecções Estreptocócicas/epidemiologia , Streptococcus/efeitos dos fármacos , Streptococcus/isolamento & purificação , Streptococcus/patogenicidade , Streptococcus pyogenes/genética , Streptococcus pyogenes/isolamento & purificação , Virulência , Adulto JovemRESUMO
BACKGROUND: We describe a case of traumatic ocular endophthalmitis caused by Nocardia kruczakiae after vegetable trauma in an immunocompetent child. FINDINGS: A 5-year-old boy suffered from a trauma with a palm tree leaflet. Two months later, he was diagnosed with traumatic infectious uveitis and intumescent cataract with anterior capsule rupture. Intensive treatment with systemic and topical vancomycin, ceftazidime and methylprednisolone began. After 1 month, he underwent phacoemulsification with intraocular lens implantation (IOL). After some episodes of reactivation, he was diagnosed with traumatic nocardial endophthalmitis from aqueous humour samples. Several operations and specific antibiotic therapy resolved the infection. CONCLUSIONS: In cases of traumatic endophthalmitis and several recurrences, it is extremely useful to make an etiologic diagnosis in order to treat the patient with specific antibiotics.