Immunosuppressive proteins isolated from spiral and coccoid cytoplasmic solutions of Helicobacter pylori.

The aim of this study was to investigate the antiproliferative proteins that probably have a role in Helicobacter pylori evade of immune response and cause chronic infection disease and also to see if coccoid form had a role in its chronicity. H. pylori strain VacA s2/m2 positive and CagA negative, from a gastric biopsy of a patient with peptic ulcer disease, was isolated and cultured in brucella agar. Both spiral and coccoid forms were harvested and ruptured by sonication. The cytoplasmic solutions of both forms were collected and their fractions obtained by gel chromatography and preparative polyacrylamide gel electrophoresis. The fractions were analyzed by MTT assay for their antiproliferative activity. We isolated two proteins with a significant dose dependent antiproliferative activity that analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis, one of them that was urease positive showed two bands with 61 and 27 kDa, which is resumed to urease of H. pylori, another consist of 57 and 63 kDa. Helicobacterpylori secret some proteins like urease that inhibit immune cells proliferation response against its antigens.

Study of nano-fiber cellulose production by Glucanacetobacter xylinum ATCC 10245.

Bacterial Celluloses (BC) are gaining importance in research and commerce due to numerous factors affecting the bacterial cellulose characteristics and application in different industries. The aim of the present study was to produce bacterial cellulose in different media using different cultivation vessels. Bacterial cellulose was produced by static cultivation of Glucanacetobacter xylinum ATCC 10245 in different culture media such as Brain Heart Agar, Luria Bertani Agar /Broth, Brain Heart Infusion, Hestrin-Schramm and medium no. 125. Cultivation of bacterium was conducted in various culture vessels with different surface area. The cellulose membrane was treated and purified with a 0.1 M NaOH solution at 90 degreesC for 30 min and dried by a freeze- drier at -40 degreesC to obtain BC. The prepared bacterial cellulose was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD). The amount of produced BC was related directly to the surface area of culture vessels.

Optimization of culture medium to increase the production of desferrioxamine B (Desferal) in Streptomyces pilosus.

The aim of this study was optimization of culture medium in direction of increasing the production rate of desferrioxamine B. Streptomycetes are the most widely studied and well known genus of the actinomycete family. Streptomycetes usually inhabit soil and are important decomposers. The genus Streptomyces are Gram-positive and GC rich bacteria that are important for production of many antibiotics and secondary metabolites. These metabolites are important in industrial and medical fields. Deferoxamines (also known as desferrioxamine B, desferoxamine B, DFO-B, DFOA, DFB or desferal) are low-molecular-weight, iron-chelating compounds (siderophores) produced and secreted by many actinomycetes, including species of Streptomyces, Nocardia and Micromonospora. Streptomyces pilosus synthesizes the siderofore desferrioxamine B. Desferrioxamine B is used clinically to treat disorders related to iron overload and pathological iron deposition in human. Our results revealed that the use of soybean as a base medium plus additives such as Na2HPO4.12H2O, NaH2PO4, MgSO4.7H2O, ZnSO4.7H2O, FeSO4.7H2O, CaCl2.2H2O, NaCl, MnSO4, NH4Cl, KH2PO4, K2HPO4, some of the amino acids and vitamins increased the production of desferrioxamine B about 8 times in comparison with the control.

Safety of human therapeutic morphine vaccine employing Lohmann specific pathogen free eggs.

One of the sensitive and standard tests to control the safety of a vaccine is the inoculation of such vaccine to the air pocket of Lohmann specific pathogen free eggs. The aim of this study is to control the safety of morphine vaccine. This study reveals the safety of morphine vaccine by employing Lohmann specific pathogen free embryo eggs. The changeable parameters in this test were: weight of eggs, safety of eggs embryo, vaccine concentration, normal saline and temperature of the incubator. To study, the safety of morphine vaccine, we used 30 eggs (after controlling the safety of eggs and their embryos) which were divided into two groups of control (15 eggs) and test (15 eggs). After weighing the eggs, the eggs under experiment were inoculated with morphine vaccine and the control group was inoculated with physiological solution. Both groups were incubated and weight of the eggs and chickens were determined accordingly. The eggs of each group were controlled by their weights showing healthy, normal growth and evolution. The comparison between the weights of control and experimental groups did not show any significant changes. Exactly growth and evolution of each group were found equally to be balancing for three weeks after injection. Finally all eggs were observed to be safe, alive and in evolutionary form. By comparing the growth and evolution amongst each egg in the group under experiment, after injection, the eggs did not show any adverse reaction after inoculation with therapeutic human morphine vaccine.

Improved production of L-lysine by over-expression of Meso-diaminopimelate decarboxylase enzyme of Corynebacterium glutamicum in Escherichia coli.

The aim of this study is over-expression of Meso-diaminopimelate decarboxylase enzyme (EC 4.1.1.20) and enhancement of L-lysine production rate. The C. glutamicum LysA gene which encodes a Meso-diaminopimelate decarboxylase was cloned in E. coli. The cloned gene was sequenced; it encodes a 445 amino acids protein with molecular weight of 47 kD. Expression of the LysA gene in E. coli resulted in an increase in Meso-diaminopimelate decarboxylase activity, correlated with the presence in sodium dodecyl sulfate-polyacrylamid gels of a clear protein band that corresponds to this enzyme. The induction of cloned gene by IPTG has been shown to have an inhibitory effect on cell growth due to over-expression of the cloned gene. A two fold increase in lysine production rate was observed after introduction of the cloned gene into E. coli.

Mutation of Streptomyces griseoflavus in order to obtain high yield desferrioxamine producing fused cells.

Streptomyces griseoflavus PTCC 1130 was mutated by UV irradiation. Two mutants were obtained (C7031 and S7011). These two mutants were able to produce desferioxamine. Desferrioxamine was extracted from the culture broth of the two mutated strains and the thin layer chromatogram of the products showed the R(F) values of 0.461, 0.463 and 0.456 for S7011, C7031 and the standard, respectively. The protoplasts of mutated Streptomyces griseoflavus were isolated and fused together. Total numbers of 58 fusions were obtained and only 17 fusions showed significant resistance to sodium azide and crystal violet. In terms of production of desferrioxamine only fusion PF9 and PF10 increased 68.3 and 81.8% desferrioxamine production as compared to parent strain (PTCC 1130), respectively.