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J Phys Chem Lett ; 12(14): 3679-3684, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33829785

RESUMO

Electron paramagnetic resonance (EPR) spectroscopy is an established technique to site-specifically monitor conformational changes of spin-labeled biomolecules. Emerging in-cell EPR approaches aiming to address spin-labeled proteins in their native environment still struggle to reach a broad applicability and to target physiologically relevant protein concentrations. Here, we present a comparative in vitro and in-cell double electron-electron resonance (DEER) study demonstrating that nanomolar protein concentrations are at reach to measure distances up to 4.5 nm between protein sites carrying commercial gadolinium spin labels.


Assuntos
Elétrons , Proteínas/análise , Espectroscopia de Ressonância de Spin Eletrônica , Células HEK293 , Humanos
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