[The role of inhalation provocation tests in the diagnosis of occupational bronchial asthma]. / Rol' ingaliatsionno-provokatsionnykh prob v diagnostike professional'noi bronkhial'noi astmy.

Light industry female workers suffering from bronchial asthma demonstrated significant pulmonary ventilation disorders of mixed type with prevalent bronchial obstruction. Those changes prove long-standing harm caused by occupational factors. Inhalation provocative tests point out leading role of industrial dust in increased bronchial reactivity.

[Effects of pesticide fluometuron (cotoran) on template synthesis of RNA]. / Vliianie pestitsida fluometurona (kotorana) na matrichnyi sintez RNK.

The experiments on the investigation of pesticide fluometuron (cotoran) influence on nuclease sensitivity and template activity of rat liver chromatin were carried out. Cotoran was found to bind specifically with non-histone proteins of chromatin. It was shown that this pesticide considerably decreases template activity of chromatin and its sensitivity to the action of nucleases. It suggests, that certain conformation changes occur in chromatin upon the action of cotoran.

[RNA synthesis and transport in the rat liver under the effects of pesticide cotoran (fluometuron)]. / Sintez i transport RNK v pecheni krys pri deistvii pestitsida kotorana (fluometurona).

The experiments on the investigation of pesticide cotoran-effect on RNA synthesis and transport were carried out. Cotoran was shown to destroy considerably the processes of RNA biosynthesis in rat liver, that results in the decrease of RNA transport from nuclei into cytoplasm. By special experiments it was established that functional activity and the integrity of nuclear membrane (according to the alteration in the activity of nuclear membrane enzyme Mg2-dependent ATP-ase) was not destroyed.

[The translocation and specific binding of the pesticide fluometuron (cotoran) with the proteins of rat liver nuclei]. / Translokatsiia i spetsificheskoe sviazyvanie pestitsida fluometurona (kotorana) s belkami iader pecheni krys.

Intracellular distribution of labeled cotoran was studied. 3H-cotoran was shown to penetrate through the nuclear membrane to accumulate uneventfully in the intranuclear components. An insignificant amount of 3H-cotoran was associated with the nucleoplasm and the outer nuclear membrane. At the same time, essential radioactivity was observed in the proteins of the nuclear matrix (up to 30%) and in non-histone proteins of chromatin (up to 60%). Acception of 3H-cotoran on metaphase chromosomes of cultured cells as well as specificity of cotoran binding with non-histone proteins of chromatin in vivo and in vitro was studied by radioautography. It was revealed that cotoran was translocated into the interphase nuclei to be accepted by metaphase chromosomes of the HeLa line cells and fibroblasts in human embryo, and specifically, in receptor-like manner, bound to chromatin proteins.

Effect of defoliant (butiphose) on morpho-physiological properties and enzyme systems of natural membranes.

Butiphose (Tributyltritiophosphate, (C4H9S)3PO) a commonly used defoliant in cotton growing regions of USSR, caused extensive alterations in morphological features of erythrocyte and nuclear membranes and affected the permeability properties of rat liver mitochondrial membrane. It disrupted Ca2+ transport system and other energy dependent processes in mitochondria. A reduction in the activity of cytochrome-c-oxidase and NAD.H-oxidase was also observed.