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1.
J Mol Cell Cardiol ; 41(4): 689-97, 2006 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-16926021

RESUMO

Cytokine systems are activated in heart failure, and it is believed that interaction between such systems may be important during progression of this disorder. We have previously shown that failing hearts have increased levels of the interleukin-6 related cytokine leukemia inhibitory factor (LIF) and activin A, a member of the transforming growth factor-beta family. The aim of this study was to examine the effects of activin A on cardiomyocytes and a potential interaction with LIF-mediated changes in cell signaling and growth. Cardiomyocytes were isolated from 1- to 3-day-old Wistar rats, and the cells were treated with LIF, activin A or a combination thereof. Our main findings were: (i) activin A treatment reduced the LIF-mediated increase in cardiomyocyte length, perimeter and sarcomeric organization and was accompanied by a substantially decreased alpha-skeletal actin gene expression. (ii) The activin A-mediated phosphorylation of Smad2 was markedly enhanced by LIF. (iii) Activin A markedly induced SOCS3 gene expression, while LIF potently increased the expression of Smad7 mRNA, representing inhibitors of LIF and activin A signaling pathways, respectively. (iv) Inhibiting activation of the Smad2/3 pathway abolished the effects of activin A on LIF-induced changes in cell length, perimeter and sarcomeric organization. In conclusion, activin A markedly attenuates LIF-induced changes in cardiomyocytes, reflecting a potentially important role for both activin A and the Smad2/3 pathway in regulation of myocardial remodeling.


Assuntos
Ativinas/farmacologia , Proteínas de Transporte/metabolismo , Fator Inibidor de Leucemia/farmacologia , Miócitos Cardíacos/metabolismo , Sarcômeros/metabolismo , Animais , Biomarcadores/análise , Cardiomegalia/tratamento farmacológico , Cardiomegalia/metabolismo , Crescimento Celular , Proliferação de Células , Células Cultivadas , Antagonismo de Drogas , Miócitos Cardíacos/efeitos dos fármacos , Ratos , Sarcômeros/efeitos dos fármacos , Transdução de Sinais , Proteína Smad2/antagonistas & inibidores , Proteína Smad2/metabolismo , Proteína Smad3/antagonistas & inibidores , Proteína Smad3/metabolismo
2.
Eur J Clin Invest ; 34(7): 451-8, 2004 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-15255781

RESUMO

BACKGROUND: Oxidative stress is a key factor in atherogenesis, in which it is closely associated with the inflammation and formation of bioactive lipids. Although 8-isoprostane is regarded as a reliable marker of oxidative stress in vivo, the pathogenic role of this F(2)-isoprostane in atherogenesis is far from clear. Based on the important role of foam cells in the initiation and progression of atherosclerosis we hereby examined the ability of 8-isoprostane to modulate oxidized (ox)LDL-induced foam cell formation and the function of these cells, particularly focusing on the effect on matrix degradation. METHODS AND RESULTS: 8-isoprostane (10 micro M) augmented the oxLDL-induced (20 micro g mL(-1)) lipid accumulation of THP-1 macrophages evaluated by Oil-Red-O staining and lipid mass quantification (colourimetric assay). Additionally, 8-isoprostane induced the expression of the scavenger receptor A type 1 (MSR-1) [mRNA and protein level], assessed by RT-PCR and Western blotting, respectively. Moreover, 8-isoprostane counteracted the oxLDL-induced apoptosis of these cells, involving both mitochondrial-protective and caspase-suppressive mechanisms. Along with these changes, 8-isoprostane increased the oxLDL-induced gene expression of matrix metalloproteinase (MMP)-9 and its endogenous inhibitor [i.e. tissue inhibitor of MMP (TIMP)-1] accompanied by enhanced total MMP activity. CONCLUSIONS: We show that 8-isoprostane increases foam cell formation at least partly by enhancing MSR-1 expression and by inhibiting apoptosis of these cells, inducing long-lived foam cells with enhanced matrix degrading capacity. Our findings further support a role for 8-isoprostane not only as a marker of oxidative stress in patients with atherosclerotic disorders, but also as a mediator in atherogenesis and plaque destabilization.


Assuntos
Dinoprosta/farmacologia , Células Espumosas/efeitos dos fármacos , Metaloproteinase 9 da Matriz/metabolismo , Receptores Imunológicos/metabolismo , Inibidor Tecidual de Metaloproteinase-1/metabolismo , Vasoconstritores/farmacologia , Apoptose , Western Blotting , Caspases/metabolismo , Dinoprosta/análogos & derivados , Células Espumosas/enzimologia , Humanos , Estresse Oxidativo/fisiologia , Receptores Depuradores
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