Biophysical insights into the interaction of human serum albumin with Cassia fistula leaf extracts inspired biogenic potent antibacterial and anticancerous gold nanoparticles.

In the present investigation, the characterization of Cassia fistula leaf extracts (CFLE) mediated gold nanoparticles (CF-GNPs) and its binding features with human serum albumin (HSA) through interaction have been probed. The results from UV-visible, TEM and EDX analysis proved the formation of CF-GNPs. The functional groups like OH, NH, CN etc present in the phytochemicals of CFLE were mainly acted as reducing and protecting agent which was confirmed by FTIR study. The zeta potential (-17.8 mV) and hydrodynamic size (20.4 nm) of the CF-GNPs were also measured by DLS. The microbicidal effect of the CF-GNPs was estimated against gram negative bacterium, Escherichia coli (DH5-Alpha) and MIC was found to be 2.8 nM. Anticancer activity of the CF-GNPs was also checked against A375 (skin melanoma) cell lines where IC50 was 6.5 nM. The interaction study of CF-GNPs with HSA and conformational alteration of HSA upon interaction were investigated by the fluorescence, lifetime, synchronous, circular dichroism spectrum and zeta potential measurement. The negative value of Gibb's free energy indicated spontaneity of the CF-GNPs-HSA complex formation. The fluorescence lifetime measurement confirmed the construction of ground state CF-GNPs-HSA complex passing through static quenching mechanism and determined the distance from donor to acceptor also. Circular dichroism spectroscopy signified unchangeable native structure of HSA with minor decrease of alpha helix structure (54.5% to 51.1%) upon interaction. The more negative zeta potential value (-25.9 mV) of CF-GNPs-HSA system than the CF-GNPs (-17.8 mV) proved the adsorption of HSA on the outer surface of CF-GNPs.Communicated by Ramaswamy H. Sarma.

Carbon dots derived from lychee waste: Application for Fe3+ ions sensing in real water and multicolor cell imaging of skin melanoma cells.

Exploit of biomass as an inexhaustible resource has accepted much more curiosity to the present research world. Herein, a simple, one-step solvothermal action has been used to synthesize an ascendable amount of fluorescent carbon dots (CDs) with an average size of~3.13 nm, from Low-reasonable and green source lychee waste. The excitation/emission maxima of CDs have 365/443 nm with high quantum yield (23.5%). The present ingredient predominantly contained carboxylic acid and hydroxyl group that acted as a passive agent for stabilizing the CDs. The structural and optical properties were evaluated through HRTEM, FTIR, UV-vis, zeta potential, XPS, fluorescence, and fluorescence lifetime experiments. We investigated the manoeuvre of our synthesized CDs as a probe for detection of Fe3+ ions in water bodies; This sensing approach showed impressive selectivity and sensitivity towards Fe3+ions with LOD 23.6 nM. The sensing mechanism took place through static quenching which was entrenched through fluorescence lifetime measurements. Fe3+ ions detection was basically carried out with efficacy in real water. For its lofty Photo-stability, low cytotoxicity and cell viability the probe were substantially applied for bio-imaging experiment i.e. intracellular multi-color cell imaging in skin melanoma cells (A375 cells) with and without Fe3+ ions exemplifying its real applications in living cells.

Binding interaction study on human serum albumin with bactericidal gold nanoparticles synthesized from a leaf extract of Musa balbisiana: a multispectroscopic approach.

This study describes the eco-friendly, low-cost and room-temperature synthesis of gold nanoparticles from Musa balbisiana leaf extract, which acts as both reducing and stabilizing agent, and characterized by ultraviolet-visible (UV-vis) light spectroscopy, fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FE-SEM), analytical transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDAX) and dynamic light scattering (DLS) instruments. These nanoparticles showed an average diameter of 33.83 ± 3.39 nm, which was confirmed from the size distribution histogram. The bactericidal activity of these nanoparticles was confirmed using bacteria Escherichia coli and Staphylococcus aureus at 1 and 2 nM minimum inhibitory concentrations, respectively. The interaction between nanoparticles and human serum albumin (HSA) was investigated, as this plays significant roles in biological systems. The nature of interaction, binding parameters and structural variation of HSA in the presence of these nanoparticles have been evaluated using several useful spectroscopic approaches such as UV-vis, FTIR, time-resolved and steady-state fluorescence, and circular dichroism in addition to the measurement of zeta potential. This interaction study revealed that static quenching occurs in this process with minimal alteration in the secondary structure, but the native structure of HSA remained unaltered. The binding constant and thermodynamic parameters of this interaction process were also evaluated.