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1.
World J Microbiol Biotechnol ; 40(1): 15, 2023 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-37975907

RESUMO

Populus deltoides is one of the most favored cash crops in northern India. Thus, accurate identification of pathogens affecting P. deltoides is a critical step in finding or developing effective control measures. In June 2020, symptoms of a leaf blight disease were observed on P. deltoides trees planted at Forest Research Institute, Dehradun, India. Calonectria-like fungal isolates were consistently isolated from the infected leaf samples. Morphological features coupled with phylogenetic analysis of combined partial actin (act), calmodulin (cmdA), histone (his3), translation elongation factor 1-alpha (tef1) and ß-tubulin (tub2) gene regions of two fungal isolates confirmed a novel species, which is described and illustrated here as Calonectria populi sp. nov. Symptoms similar to those observed in natural conditions were caused by both the isolates on P. deltoides clone AM109 in detached leaf assays and glasshouse inoculation experiments. Finally, Koch's postulates were established by re-isolation and re-identification of the pathogen from the inoculated leaves. This work is the first to confirm a new leaf blight disease of P. deltoides caused by C. populi sp. nov. in India and worldwide.


Assuntos
Hypocreales , Populus , Filogenia , Actinas , Bioensaio , Índia
3.
Acta Biomater ; 31: 71-79, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26654763

RESUMO

(RADA)4 peptides are promising biomaterials due to their high degree of hydration (<99.5% (w/v)), programmability at the molecular level, and their subsequent potential to respond to external stimuli. Interestingly, these peptides have also demonstrated the ability to cause rapid (∼15s) hemostasis when applied directly to wounds. General hemocompatibility of (RADA)4 nanofibers was investigated systematically using clot formation kinetics, C3a generation, and platelet activation (morphology and CD62P) studies. (RADA)4 nanofibers caused a rapid clot formation, but yielded a low platelet activation and low C3a activation. The study suggests that the rapid hemostasis observed when these materials are employed results principally from humoral coagulation, despite these materials having a net neutral charge and high hydration at physiological conditions. The observed rapid hemostasis may be induced due to the available nanofiber surface area within the hydrogel construct. In conclusion, our experiments strongly support further development of (RADA)4 peptide based biomaterials. STATEMENT OF SIGNIFICANCE: Biomedicine based applications of (RADA)4 peptides are being extensively studied for the purpose of improving drug carriers, and 3D peptide nanofiber scaffolds. However, this peptide's biocompatibility has not been investigated till now. One particular study has reported a revolutionary and very desirable ability of (RADA)4 peptide to achieve complete and rapid hemostasis, nevertheless, the literature remains inconclusive on the underlying molecular mechanism. In this manuscript we bridge these two main knowledge gaps by providing the much needed systematic biocompatibility analysis (morphology analysis, platelet and C3a activation) of the (RADA)4 based hydrogels, and also investigate the underlying hemostatic mechanism of this peptide-induced hemostasis. Our work not only provides the much-needed biocompatibility of the peptide for applicative research, but also explores the molecular mechanism of hemostasis, which will help us design novel biomaterials to achieve hemostasis.


Assuntos
Hemostasia , Hidrogéis/química , Peptídeos/química , Engenharia Tecidual/métodos , Materiais Biocompatíveis/química , Coagulação Sanguínea , Complemento C3/química , Complemento C3a/química , Proteínas do Sistema Complemento , Portadores de Fármacos , Sistemas de Liberação de Medicamentos , Humanos , Inflamação , Cinética , Teste de Materiais , Microscopia Eletrônica de Transmissão , Nanofibras/química , Nefelometria e Turbidimetria , Selectina-P/química , Ativação Plaquetária
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