RESUMO
We have identified a number of transcribed sequences within a 400-kb interval on chromosome 11p15.1--> p14. Six genes and 13 novel transcripts including ESTs, cDNAs and exons have been identified and assigned to this region. Comparison of mRNA sequence with genomic sequence has enabled us to determine the exon/intron structure of four of the genes (NUCB2, PIK3C2A, RPS13 and OR7E14P).
Assuntos
Cromossomos Humanos Par 11/genética , Genes , Mapeamento Físico do Cromossomo , Transcrição Gênica/genética , Sequência de Bases , Clonagem Molecular , DNA Complementar/genética , Éxons/genética , Etiquetas de Sequências Expressas , Ordem dos Genes/genética , Humanos , Hibridização in Situ Fluorescente , Íntrons/genética , Família Multigênica/genética , Pseudogenes/genética , RNA Mensageiro/análise , RNA Mensageiro/genéticaRESUMO
A very stable chelating resin matrix was synthesized by covalently linking o-vanillinthiosemicarbazone (oVTSC) with the benzene ring of the polystyrene-divinylbenzene resin Amberlite XAD-2 through a -NN- group. The resin was used successfully for the separation and preconcentration of copper(II), zinc(II) and lead(II) prior to their determination by atomic absorption spectrophotometry. The total sorption capacity of the resin was 850, 1500 and 2000 mug g(-1) of the resin for Cu(II), Zn(II) and Pb(II), respectively. For the quantitative sorption and recovery of Cu(II), Zn(II) and Pb(II), the optimum pH and eluants were pH 2.5-4.0 and 4 M HCl or 2 M HNO(3) for Cu(II), pH 5.5-6.5 and 1.0-2.0 M HCl for Zn(II) and pH 6.0-7.5 and 3 M HCl or 1 M HNO(3) for Pb(II). Both, the uptake and stripping of these metal ions were fairly rapid, indicating a better accessibility of the chelating sites. The t (1 2 ) values for Cu(II), Zn(II) and Pb(II) were also determined. Limit of tolerance of some electrolytes like NaCl, NaF, NaNO(3), Na(2)SO(4) and Na(3)PO(4) have been reported. The preconcentration factor for Cu(II), Zn(II) and Pb(II) was 90, 140 and 100 respectively. The method was applied for the determination of Cu(II), Zn(II) and Pb(II) in the water samples collected from Sabarmati river, Ahmedabad, India.
RESUMO
The human carbonic anhydrase (CA) VIII gene (CA8) has been mapped to chromosome 8 at q11-->q12 by human-mouse hybrid mapping and by fluorescence in situ hybridization. The closely-linked human CA isozyme genes, CA1, CA2 and CA3, are also located on chromosome 8, but at q22, and therefore not closely linked to the CA8 locus.
