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1.
Transplant Proc ; 49(1): 61-64, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28104160

RESUMO

BACKGROUND: Transplant kidney function is thought to be affected by sex differences, such as physical conditions including muscle volume, sex hormones, immune responses, and so forth. We examined the effect of sex differences on transplant kidney function. METHODS: The subjects were selected from kidney transplant recipients, who received kidney transplantation on our hospital between January 2000 and August 2015. Cadaveric donors and parent-child pairs with an age difference were excluded, then we included 47 recipients whose sex was different from the sex of the donor. We compared transplant kidney function between male donors and female recipients group (M→F, n = 20) and female donors and male recipients group (F→M, n = 27). RESULTS: Nadir creatinine value was higher in the F→M group than in the M→F group (1.09 mg/dL vs 0.76 mg/dL, P < .0001). The estimated glomerular filtration rate (eGFR) was significantly higher in the M→F group than in the F→M group (66.6 mL/min/1.73 m2 vs 50.1 mL/min/1.73 m2, P = .002), and eGFR ratio (recipient to donor) was significantly higher in the M→F group than in the F→M group (1.13 vs 0.57, P < .0001). Multiple linear regression analysis showed that the only the sex of the recipient was significant prognostic factor of eGFR after renal transplantation (P = .037). CONCLUSIONS: The short-term kidney function of the graft from male to female was better than that of the graft from female to male.


Assuntos
Transplante de Rim , Caracteres Sexuais , Doadores de Tecidos , Adulto , Feminino , Taxa de Filtração Glomerular , Humanos , Rim/fisiologia , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Estudos Retrospectivos
2.
Transplant Proc ; 46(2): 620-2, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24656028

RESUMO

A 50-year-old man, who had received an ABO-incompatible living related preemptive renal transplantation 1 year before, presented with painful lesions on both lower extremities and fever. At first, bacterial cellulitis was suspected and antibiotic therapy was initiated, but it was not effective. The serum cryptococcal antigen titer was 1:4,098, and pathologic examination of debrided tissue and wound pus culture revealed cryptococcal necrotizing fasciitis. Liposomal amphotericin B and fluconazole were started, and repeated debridement and skin grafting were performed. Because his graft function deteriorated because of antibody-mediated rejection and polyoma viral nephropathy, hemodialysis was induced on day 9 of hospitalization. During the treatment, he suffered repeated urinary tract infections, which were treated with antibiotics, and cytomegalovirus retinopathy, which was treated with ganciclovir. His cryptococcal necrotizing fasciitis was successfully cured by the combination of antimicrobial treatment and surgical procedures. He could walk with a cane and was discharged on day 298 of hospitalization. Cryptococcal necrotizing fasciitis in renal transplant recipients is so rare that only 14 cases have been reported. The mortality is not very high, but the prognosis of the patient is complicated by worsening of the cryptococcal infection of the central nervous system (CNS). Early detection and treatment to prevent spreading to other sites, especially the CNS or disseminated disease, is very important in cases of cryptococcal necrotizing fasciitis.


Assuntos
Criptococose/complicações , Fasciite Necrosante/complicações , Transplante de Rim/efeitos adversos , Anfotericina B/administração & dosagem , Anfotericina B/uso terapêutico , Criptococose/tratamento farmacológico , Quimioterapia Combinada , Fasciite Necrosante/tratamento farmacológico , Fasciite Necrosante/microbiologia , Fluconazol/administração & dosagem , Fluconazol/uso terapêutico , Humanos , Masculino , Pessoa de Meia-Idade
3.
Eur J Phys Rehabil Med ; 44(4): 407-15, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19002090

RESUMO

AIM: In contrast to the single evaluation methods used in the past, the combination of multiple tests allows one to obtain a global assessment of the ankle joint. The aim of this study was to determine the reliability of the different tests in a functional test battery. METHODS: Twenty-four male recreational athletes with unilateral functional ankle instability (FAI) were recruited for this study. One component of the test battery included five different functional ability tests. These tests included a single limb hopping course, single-legged and triple-legged hop for distance, and six and cross six meter hop for time. The ankle joint position sense and one leg standing test were used for evaluation of proprioception and sensorimotor control. The isokinetic strengths of the ankle invertor and evertor muscles were evaluated at a velocity of 120 degrees /s. The reliability of the test battery was assessed by calculating the intraclass correlation coefficient (ICC). Each subject was tested two times, with an interval of 3-5 days between the test sessions. RESULTS: The ICCs for ankle functional and proprioceptive ability showed high reliability (ICCs ranging from 0.94 to 0.98). Additionally, isokinetic ankle joint inversion and eversion strength measurements represented good to high reliability (ICCs between 0.82 and 0.98). CONCLUSION: The functional test battery investigated in this study proved to be a reliable tool for the assessment of athletes with functional ankle instability. Therefore, clinicians may obtain reliable information from the functional test battery during the assessment of ankle joint performance in patients with functional ankle instability.


Assuntos
Traumatismos do Tornozelo/reabilitação , Articulação do Tornozelo/fisiopatologia , Instabilidade Articular/fisiopatologia , Força Muscular , Propriocepção , Esportes/fisiologia , Traumatismos do Tornozelo/fisiopatologia , Teste de Esforço , Humanos , Músculo Esquelético/fisiopatologia , Amplitude de Movimento Articular , Reprodutibilidade dos Testes , Entorses e Distensões/fisiopatologia , Entorses e Distensões/reabilitação , Análise e Desempenho de Tarefas
4.
Endocr J ; 48(1): 103-11, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11403095

RESUMO

A rare case of aldosterone-producing adrenocortical adenoma with preclinical Cushing's syndrome and hypersecretion of parathyroid hormone (PTH) is described. A 64-year-old male patient had a history of hypertension for two decades and hypokalemia for 4 years. He suffered from left hemiparesis and aphasia due to cerebral hemorrhage, but his appearance was not Cushingoid. His plasma renin activity was below the normal range, while plasma aldosterone concentration was high. They did not respond to furosemide-upright test. His plasma cortisol level in the morning was at the upper limit of the normal range, but it did not show a diurnal rhythm nor was it suppressed by 1 mg and 8 mg of dexamethasone. Computed tomography showed a low density tumor in the right adrenal gland. An adrenal scintigram under dexamethasone treatment revealed an uptake of the tracer on the right side, and plasma aldosterone and cortisol concentrations in the adrenal vein were higher on the right side than on the opposite. The diagnosis of right aldosterone-producing adrenal adenoma with an autonomous production of cortisol was confirmed by right adrenalectomy. Histological findings showed an adenoma consisting mostly of clear cells, but that the nests of compact cells were scattered. Analysis of an extract from the adenoma revealed that the adenoma contained an excess amount of aldosterone and that the cortisol/corticosterone ratio was higher than that of aldosterone-producing adenoma. Both serum calcium and PTH levels remained high one year after adrenalectomy. Ultrasonography revealed the swelling of a parathyroid gland on the left side, indicating the coexistence of an autonomous hyperparathyroidism.


Assuntos
Adenoma/diagnóstico , Neoplasias do Córtex Suprarrenal/diagnóstico , Aldosterona/biossíntese , Síndrome de Cushing/diagnóstico , Hormônio Paratireóideo/metabolismo , Adenoma/fisiopatologia , Adenoma/cirurgia , Neoplasias do Córtex Suprarrenal/fisiopatologia , Neoplasias do Córtex Suprarrenal/cirurgia , Adrenalectomia , Hormônio Adrenocorticotrópico/sangue , Aldosterona/sangue , Cálcio/sangue , Ritmo Circadiano , Hormônio Liberador da Corticotropina , Síndrome de Cushing/fisiopatologia , Dexametasona , Glucocorticoides , Humanos , Hidrocortisona/sangue , Masculino , Pessoa de Meia-Idade , Renina/sangue , Tomografia Computadorizada por Raios X
5.
Pediatr Nephrol ; 14(8-9): 794-6, 2000 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10955929

RESUMO

The number of patients with membranoproliferative glomerulonephritis(MPGN) diagnosed in our institution and the incidence of hypocomplementemia as determined by school urinary screening programs in Japan were investigated retrospectively. Fifty-seven children were diagnosed as having MPGN between 1974 and 1997. Of these, 13 patients were diagnosed during period 1 (1974-1979) and 25 during period 2 (1980-1985). A decreasing trend was observed during periods 3 (1986-1991) (12 patients) and 4 (1992-1997) (7 patients) compared with period 2 (P<0.05 and P<0.01, respectively). A significant difference was also noted when MPGN was compared between periods 1 and 2 (1974-1985) and periods 3 and 4 (1986-1997) (P<0.01). Serum C3 was measured in 1,282 school children with abnormal urinary findings between 1980 and 1997. Thirty children had hypocomplementemia. The incidence of hypocomplementemia was significantly lower in period 3 (1986-1991) (9 children) and period 4 (1992-1997) (2 children) compared with period 2 (1980-1985) (19 children) (P<0.05 and P<0.01, respectively). New cases of MPGN in our institution and the incidence of hypocomplementemia as determined by school urinary screening programs are decreasing in Japan. The reason for this trend is unknown.


Assuntos
Complemento C3/deficiência , Proteínas do Sistema Complemento/deficiência , Glomerulonefrite Membranoproliferativa/diagnóstico , Glomerulonefrite Membranoproliferativa/epidemiologia , Adulto , Criança , Complemento C3/análise , Proteínas do Sistema Complemento/análise , Europa (Continente)/epidemiologia , Glomerulonefrite Membranoproliferativa/urina , Humanos , Incidência , Japão/epidemiologia , Programas de Rastreamento , Estudos Retrospectivos , Estados Unidos/epidemiologia
6.
Eur J Neurosci ; 11(9): 3355-8, 1999 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-10510202

RESUMO

The neuronal circuitry through which prolonged excitation is generated in the spinal dorsal horn was investigated using optical imaging of neuronal excitation in transverse slices of rat spinal cords. It is known that tetanic stimulation (20 Hz for 1 s) of the dorsal root that activates both A and C primary afferent fibres elicits slow intrinsic optical signals (IOS) in the dorsal horn, seen most intensely in the substantia gelatinosa (SG), lamina II, and that IOS expresses in part the slow synaptic response recorded intracellularly in dorsal horn neurons. We here report that the slow IOS within the SG were completely abolished after an incision was made at the border between the SG and the deeper laminae, but not after an incision within the deeper dorsal horn of the laminae III-V. The result demonstrates directly that, in order to generate prolonged excitation in the SG, the neuronal elements in the deeper dorsal horn must be intact. Thus, the afferent information might be received first by the deeper elements and then transmitted to the SG region, and/or collaboration between the SG and deeper elements is necessary to maintain prolonged excitation in the SG.


Assuntos
Rede Nervosa/fisiologia , Células do Corno Posterior/fisiologia , Animais , Estimulação Elétrica , Eletrofisiologia , Soluções Hipotônicas , Rede Nervosa/citologia , Neurônios Aferentes/fisiologia , Estimulação Luminosa , Ratos , Ratos Sprague-Dawley , Substância Gelatinosa/fisiologia , Transmissão Sináptica/fisiologia
7.
Mol Gen Genet ; 262(4-5): 781-9, 1999 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-10628861

RESUMO

The Escherichia coli gene recA is essential for homologous recombination and DNA repair, and homologs have been identified in eukaryotes. A basidiomycete, Coprinus cinereus, which has many advantages for the study of meiosis, was recently reported to have a homolog of one of these, RAD51. In the yeast Saccharomyces, mutations in the RAD51 gene cause defects in both somatic and meiotic cells. Based on this finding, we screened for a meiosis-specific homolog of recA, equivalent to Lilium LIM15 or Saccharomyces DMC1, in C. cinereus, and isolated a clone containing a 1.2-kb DNA fragment from a cDNA library constructed with Coprinus poly(A)+ RNA isolated from cells undergoing meiosis. The predicted amino acid sequence was 52% identical to the putative gene product of the lily cDNA clone LIM15 and 61% identical to Saccharomyces DMC1, and showed limited sequence similarity to the products of RAD52, 55, and 57. The synchrony of meiosis in Coprinus provides an ideal system for the investigation of differential gene expression in relation to meiosis and fruiting body development. Northern analysis indicated that Coprinus LIM15/DMC1 was expressed at meiotic prophase within 8 h after the onset of karyogamy, suggesting that the gene functions mostly at the stage at which the homologous chromosomes pair, but may not be essential at the point at which they recombine. The gene is not expressed in somatic cells.


Assuntos
Proteínas de Ciclo Celular , Cromossomos Fúngicos , Coprinus/genética , Proteínas de Ligação a DNA/genética , Proteínas Fúngicas/genética , Meiose/genética , Sequência de Aminoácidos , Sequência de Bases , Northern Blotting , Southern Blotting , Primers do DNA , DNA Fúngico , Dados de Sequência Molecular , RNA Mensageiro/genética , Proteínas de Saccharomyces cerevisiae , Homologia de Sequência de Aminoácidos
8.
Neuroreport ; 9(16): 3663-7, 1998 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-9858376

RESUMO

Tetanic stimulation of high-threshold primary afferent fibers in the dorsal root was found to elicit intrinsic optical signals (IOSs) in transverse slices of 11- to 20-day-old rat spinal cords. The IOS, lasting for 30 s or longer, was most prominent in the lamina II of the dorsal horn. Treatment with a Na+-K+-2Cl- co-transport blocker, furosemide, abolished the IOS, suggesting that the origin of the IOS is the cellular swelling due to an activity-dependent rise in extracellular K+. Substance P antagonist spantide, glutamate antagonists 2-amino-5-phosphonovaleric acid and 6-cyano-7-nitroquinoxaline-2,3-dione, and the mu-opioid agonist [d-Ala2,N-Me-Phe4,Gly5-ol]-enkephalin suppressed IOSs. Thus, IOSs represent at least in part the slow excitatory response that is known to be generated in dorsal horn neurons after tetanic activation of unmyelinated afferent fibers.


Assuntos
Neurônios Aferentes/fisiologia , Substância Gelatinosa/citologia , Substância Gelatinosa/fisiologia , 2-Amino-5-fosfonovalerato/farmacologia , 6-Ciano-7-nitroquinoxalina-2,3-diona/farmacologia , Potenciais de Ação/efeitos dos fármacos , Potenciais de Ação/fisiologia , Analgésicos/farmacologia , Analgésicos Opioides/farmacologia , Animais , Corantes , Diuréticos/farmacologia , Estimulação Elétrica , Ala(2)-MePhe(4)-Gly(5)-Encefalina , Encefalinas/farmacologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/fisiologia , Furosemida/farmacologia , Gânglios Espinais/fisiologia , Dor/fisiopatologia , Ratos , Substância P/análogos & derivados , Substância P/farmacologia , Substância Gelatinosa/efeitos dos fármacos
9.
Int J STD AIDS ; 9(7): 414-7, 1998 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-9696198

RESUMO

A clinical study of patients with male urethritis (n=316) was undertaken to determine the sensitivity potential for a new dual amplified immunoassay (IDEIA PCE Chlamydia). Increased sensitivity (98.8%, 84/85) was obtained for IDEIA PCE Chlamydia compared to a conventional antigen detection test (IDEIA Chlamydia, 81.2%, 69/85) when testing urine samples. In a smaller patient population (n=104) the positivity rate for the first-void urine tested with IDEIA PCE Chlamydia of 30.8% (32/104) was similar to the 27.9% (29/104) obtained from urethral swabs tested with a DNA probe assay (PACE 2). The increased sensitivity of the test was confirmed with a commercial PCR kit (Amplicor) and nested PCR. The IDEIA PCE Chlamydia kit has the sensitivity potential to be a clinically reliable alternative for detecting Chlamydia trachomatis.


Assuntos
Infecções por Chlamydia/diagnóstico , Doenças dos Genitais Masculinos/diagnóstico , Imunoensaio/métodos , Uretrite/diagnóstico , Infecções por Chlamydia/urina , Chlamydia trachomatis/isolamento & purificação , Estudos de Avaliação como Assunto , Doenças dos Genitais Masculinos/urina , Humanos , Masculino , Sensibilidade e Especificidade , Uretrite/urina
10.
Auris Nasus Larynx ; 24(1): 85-90, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-9148733

RESUMO

The relationship of nucleolar organizer regions (NORs) to proliferative activity and tumor progression was studied in 16 supraglottic carcinomas. The number of NORs stained by a silver colloid staining method (AgNOR staining method) was determined. The mean AgNOR number tended to be higher (but not significantly so) in poorly differentiated tumors. Moreover as T and N categories and stage of the tumor rose, the AgNOR number also rose, but not significantly. More interestingly, the mean AgNOR number was significantly higher in the presence than in the absence of lymph node metastasis. These studies indicate that the AgNOR number might be of clinical value as a predictor of lymph node metastasis of supraglottic carcinomas.


Assuntos
Carcinoma de Células Escamosas/patologia , Glote/patologia , Neoplasias Laríngeas/patologia , Metástase Linfática , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Estudos Prospectivos , Taxa de Sobrevida
11.
Auris Nasus Larynx ; 22(3): 200-4, 1995.
Artigo em Inglês | MEDLINE | ID: mdl-8561703

RESUMO

A rare case of laryngectomee who acquired TE shunt speech is reported. Our patient was a 64-year-old woman with laryngeal cancer (glottic type T3N0M0). She underwent laryngectomy in 1990, and a Komorn tracheo-esophageal shunt operation was performed simultaneously. A voice prosthesis (BIVONA) was inserted into the shunt, but the voice valve was not used. She acquired shunt speech soon after the operation, and there was no need to close the tracheostoma during phonation. Fiberoptic, X-ray and aerodynamic findings of the shunt and esophagus were as follows: (i) The one-way valve of the voice prosthesis was open during inspiration and closed during phonation. (ii) Swallowing of air, as seen in esophageal speech, was not observed. (iii) The neoglottis was in the upper part of the esophagus. It was concluded that the air for the vibration of the neoglottis entered the esophagus through the T-E shunt during inspiration.


Assuntos
Laringectomia , Voz Alaríngea , Voz Esofágica , Feminino , Humanos , Neoplasias Laríngeas/patologia , Neoplasias Laríngeas/cirurgia , Laringe/patologia , Laringe/cirurgia , Laringe Artificial , Masculino , Pessoa de Meia-Idade
12.
Eur Arch Otorhinolaryngol ; 252(8): 499-503, 1995.
Artigo em Inglês | MEDLINE | ID: mdl-8719595

RESUMO

The significance of nucleolar organizer regions (NORs) and nuclear DNA content in 73 glottic carcinomas was assessed for proliferative activity and tumor progression. NORs stained with silver colloid were counted, and nuclear DNA content was assayed by cytofluorometry. The cytofluorometric study demonstrated that the percentage of tumors with aneuploidy tended to increase as histological differentiation decreased. Survival rates of patients with diploid and aneuploid tumors were not significantly different. AgNOR staining revealed that mean AgNOR numbers rose as histological differentiation of tumors decreased. Moreover, as T and N categories and stages showed advancing malignancy, mean AgNOR numbers tended to rise. However, there was no significant difference in survival rates between tumors with low and with high AgNOR counts. These studies indicate that while AgNOR staining is better than DNA cytofluorometry for determining histological differentiation of glottic carcinoma, neither is of prognostic value at the present time.


Assuntos
Carcinoma/ultraestrutura , DNA de Neoplasias , Citometria de Fluxo , Glote/ultraestrutura , Neoplasias Laríngeas/ultraestrutura , Carcinoma/patologia , Movimento Celular , Técnicas de Cultura , Glote/patologia , Humanos , Neoplasias Laríngeas/patologia , Ploidias
13.
Eur Arch Otorhinolaryngol ; 252(3): 176-80, 1995.
Artigo em Inglês | MEDLINE | ID: mdl-7662354

RESUMO

In this study of proliferative activity of adenoid cystic carcinomas (ACCs) with different histological patterns, the argyrophilic staining method of nucleolar organizer regions (AgNORs) was applied to paraffin sections from 25 tumors. Sixteen of the neoplasms showed a mixture of different histological patterns in the same section. The AgNOR numbers in the different histological pattern areas in the same tissue sections of all 16 tumors were lowest in the cribriform, intermediate in the trabecular, and highest in the solid pattern areas. The mean AgNOR number was highest in the solid pattern (3.33 +/- 0.52), and lowest in the cribriform pattern (2.12 +/- 0.43). The results show the order of proliferative activity in the different histological pattern areas of ACC, and explain the previous clinical finding that the prognosis of ACC is poorest in solid pattern ACCs. AgNOR staining appears to be a useful technique for evaluation of the proliferative activity of each histological pattern area of ACC, especially when there are different histological pattern areas in the same tissue section.


Assuntos
Carcinoma Adenoide Cístico/ultraestrutura , Região Organizadora do Nucléolo/ultraestrutura , Neoplasias das Glândulas Salivares/ultraestrutura , Adulto , Idoso , Carcinoma Adenoide Cístico/patologia , Divisão Celular , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Neoplasias das Glândulas Salivares/patologia , Coloração pela Prata
14.
Hinyokika Kiyo ; 40(6): 475-8, 1994 Jun.
Artigo em Japonês | MEDLINE | ID: mdl-8073953

RESUMO

Ultrasound-guided percutaneous puncture of renal cysts replaced by CO2 was performed on 9 renal cysts of 6 patients from May 1993 through September 1993. Puncture and aspiration of cysts was performed using a 5.5 Fr one-step fluid drain kit (pig tail stent) under ultrasonography inspection. Aspirated volume of cyst contents was 15-292ml (mean 115 ml), and the instilled CO2 volume was 15-150 ml (mean 73 ml). All patients were checked by CT scan. In one month, the reduction rate of cyst volume was 46-84% (mean 69%) and lumbago, their chief complaint, was improved in all patients. No complications were observed in patients treated by CO2 instillation. Although long-term followup is needed to draw a definite conclusion, this therapy may be useful for benign renal cystic lesions.


Assuntos
Dióxido de Carbono/administração & dosagem , Doenças Renais Císticas/terapia , Punções/métodos , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Injeções Intralesionais , Doenças Renais Císticas/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Sucção , Tomografia Computadorizada por Raios X , Ultrassonografia
15.
Nihon Jibiinkoka Gakkai Kaiho ; 97(6): 1034-40, 1994 Jun.
Artigo em Japonês | MEDLINE | ID: mdl-8051591

RESUMO

Some papillary carcinomas of thyroid glands contain not only typical papillary structures but also various histological features in the same tumor. To investigate how areas with variable histological features differ in proliferative activity and how these differences influence the biological behavior of the whole tumor, we measured DNA contents by cytofluorometry in six papillary carcinomas with different histological features in the same tumor. DNA ploidy patterns were determined and the percentage of S plus G2M phase cells (S + G2M fraction) was calculated only in diploid tumors to assess the proliferative activity. The following conclusions were drawn. 1) It was demonstrated that there were differences in proliferative activity among areas with variable histological features in 4 of six tumors. 2) In the tumors which invaded adjacent tissues, the proliferative activity of the invasive area tended to be higher than that of the original area of the tumor. Therefore, the possibility exists that more precise evaluation of the biological behavior of the tumor can be obtained by measuring DNA contents of the tumor cells in invasive areas. 3) There was the gap in S + G2M fractions among some tumors with the same histological differentiation. Accordingly, DNA analysis of tumors would be of value in estimating proliferative activity.


Assuntos
Carcinoma Papilar/química , DNA de Neoplasias/análise , Neoplasias da Glândula Tireoide/química , Idoso , Idoso de 80 Anos ou mais , Carcinoma Papilar/patologia , Divisão Celular , Feminino , Citometria de Fluxo , Humanos , Pessoa de Meia-Idade , Ploidias , Neoplasias da Glândula Tireoide/patologia
16.
Nihon Jinzo Gakkai Shi ; 36(2): 123-9, 1994 Feb.
Artigo em Japonês | MEDLINE | ID: mdl-8139144

RESUMO

Cyclosporine A (CYA) a new immunosuppressive drug, has greatly improved the outcome of solid graft transplantation. The nephrotoxicity of CYA, however, is a serious problem, and constitutes the major obstacle limiting the use of CYA as an immunosuppressive agent for renal transplantation. On the other hand, the newly described atrial natriuretic peptide (ANP) hormonal system in both humans and animals appears to play an important role in sodium and water excretion. The present study examined the nephrotoxicity of CYA and the effect of recombinant-ANP (r-ANP) on the damaged kidneys in three groups. Group 1 consisted of rats with 45 minutes warm ischemic left kidney and right nephrectomy [WI rats]; Group 2 consisted of WI rats with oral administration of CYA (100mg/kg/day) for 2 weeks [WI+CYA rats]; Group 3 consisted of WI+CYA rats with intraperitoneal administration of r-ANP (10ng/kg/day) for 2 weeks. Group 2 had lower glomerular filtration rates (GFR) and renal plasma flow (RPF) than Group 1, but Group 3 had significantly higher GFR and RPF than Group 2. Additionally, in order to demonstrate the change in renal viability among the three groups, the adenosine triphosphoric acid level (ATP) and adenylate energy charge ratio (EC) of renal parenchymal tissue were measured by high performance liquid chromatography (HPLC). Group 2 had lower ATP and EC than Group 1, and Group 3 had higher ATP and EC than Group 2. The results of this study suggest that r-ANP is efficacious on rat kidney damage induced by CYA and WIT.


Assuntos
Fator Natriurético Atrial/farmacologia , Ciclosporina/toxicidade , Isquemia/prevenção & controle , Rim/efeitos dos fármacos , Animais , Rim/irrigação sanguínea , Masculino , Ratos , Ratos Endogâmicos F344 , Proteínas Recombinantes/farmacologia
17.
Acta Otolaryngol ; 113(4): 568-74, 1993 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-8379314

RESUMO

Precancerous lesions and carcinomas were produced in rat tongues by oral administration of 0.001% 4-nitroquinoline 1-oxide (4NQO) in drinking water, and the biological characteristics and tumor kinetics were studied. Bromodeoxyuridine labeling index (BrdU L.I.) was determined immunohistochemically, and the nuclear DNA content was measured by DNA cytofluorometry. The BrdU L.I. was lowest in normal epithelium (3.49 +/- 0.49%), higher in papillomas (8.24 +/- 1.76%) and highest in squamous cell carcinomas (13.36 +/- 4.40%). Normal lingual epithelial cells, papillomas and 18 of the 25 squamous cell carcinomas showed a diploid pattern, while 7 squamous cell carcinomas showed a diploid plus tetraploid pattern. The correlation between polyploidization and tumor progression was significant, but the correlation between BrdU L.I. and tumor progression was not significant. The results suggest that while polyploidization of tumors increases with tumor progression the growth rate of tumors does not necessarily do so.


Assuntos
Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Papiloma/genética , Papiloma/patologia , Neoplasias da Língua/genética , Neoplasias da Língua/patologia , 4-Nitroquinolina-1-Óxido/efeitos adversos , Animais , Bromodesoxiuridina , Carcinoma de Células Escamosas/induzido quimicamente , Ciclo Celular , Divisão Celular , Diploide , Epitélio/efeitos dos fármacos , Epitélio/patologia , Citometria de Fluxo , Fase G2 , Imuno-Histoquímica , Masculino , Mitose , Papiloma/induzido quimicamente , Poliploidia , Ratos , Ratos Sprague-Dawley , Fase S , Neoplasias da Língua/induzido quimicamente
18.
Gan To Kagaku Ryoho ; 20(6): 839-42, 1993 Apr.
Artigo em Japonês | MEDLINE | ID: mdl-8489295

RESUMO

Eleven tongue papillomas and 25 tongue carcinomas were produced in rats by the oral administration of 0.001% 4-nitroquinoline 1-oxide (4 NQO) in drinking water, and tumor kinetics were investigated by both DNA cytofluorometry and Ag-nucleolar organizer regions (Ag-NORs) staining. The histopathologic characteristics, the mean Ag-NOR number per cell and DNA ploidy patterns were compared. The mean Ag-NOR number was least in normal epithelium, more numerous in papillomas and highest in squamous cell carcinomas. The differences were statistically significant. All cases of normal epithelium and papilloma showed a diploid pattern. Eighteen cases of the squamous cell carcinomas (72%) showed a diploid pattern, and 7 cases (28%) exhibited a diploid plus tetraploid pattern. There was a significant difference in the mean Ag-NOR number between the two groups of squamous cell carcinomas. These results suggest that the mean Ag-NOR number may reflect the cell kinetics in the process of carcinogenesis and polyploidization of carcinomas.


Assuntos
Carcinoma de Células Escamosas/patologia , DNA de Neoplasias/análise , Região Organizadora do Nucléolo/patologia , Papiloma/patologia , Neoplasias da Língua/patologia , Animais , Carcinoma de Células Escamosas/genética , Divisão Celular , DNA de Neoplasias/genética , Citometria de Fluxo , Masculino , Papiloma/genética , Ploidias , Ratos , Ratos Sprague-Dawley , Coloração pela Prata , Neoplasias da Língua/genética
19.
Nihon Jibiinkoka Gakkai Kaiho ; 96(3): 409-14, 1993 Mar.
Artigo em Japonês | MEDLINE | ID: mdl-8386231

RESUMO

The histological features of adenoid cystic carcinoma are varied. In general, the tumors are classified into 4 histological patterns: tubular, cribriform, trabecular and solid. Numerous previous reports have indicated that the tubular pattern usually represents a favorable prognosis, the solid pattern a poor prognosis and the cribriform pattern an intermediate prognosis. Therefore, the present study was undertaken to determine precisely the proliferative potential of each histological pattern of adenoid cystic carcinomas. A silver colloid technique to identify nucleolar organizer region associated protein (AgNORs) was applied to paraffin sections in a total of 16 adenoid cystic carcinomas. A morphometric analysis of highly magnified photographic images of AgNORs in light microscopic preparations was performed. Of the 16 tumors, 8 showed a mixture of different histological patterns in the same section. In comparing the AgNOR number among different histological patterns in the same section, the value was highest for the solid pattern, lowest for the cribriform pattern and intermediate for the trabecular pattern. Moreover, the mean AgNOR number also showed a stepwise increase from the cribriform pattern (2.3 +/- 0.3) through the trabecular pattern (2.9 +/- 0.2) to the solid pattern (3.3 +/- 0.6). There was a significant difference in AgNOR numbers between cribriform and trabecular and between cribriform and solid patterns. Our results indicate that the proliferative potential of histological patterns of adenoid cystic carcinoma is lowest in the cribriform, highest in the solid, and intermediate in the trabecular pattern area. The AgNOR staining technique appears to be of value in estimating the proliferative activity of adenoid cystic carcinomas.


Assuntos
Carcinoma Adenoide Cístico/patologia , Neoplasias de Cabeça e Pescoço/patologia , Região Organizadora do Nucléolo/patologia , Adulto , Idoso , Carcinoma Adenoide Cístico/classificação , Divisão Celular , Humanos , Pessoa de Meia-Idade , Coloração pela Prata
20.
J Laryngol Otol ; 106(11): 1016-20, 1992 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-1336026

RESUMO

Eight cases of adenoid cystic carcinoma were reviewed to determine whether and how the histological features of the tumour vary with the progress of the disease. The tumours were classified by their histological patterns as tubular, cribriform, trabecular or solid. The relative amount of each pattern seen in routine light microscopic sections was calculated histomorphometrically and compared in the primary tumour and recurrent and/or metastatic lesions in the same case. In the early stage, the tubular pattern predominated. Later, the highest percentage shifted to the cribriform, then to the trabecular and finally, in the late stage, to the solid pattern. There was no reverse direction of histological transformation from the solid to the tubular pattern. These results may help to explain previous reports that the tubular pattern usually represents a favourable prognosis, the solid pattern a poor prognosis and the cribriform pattern an intermediate prognosis.


Assuntos
Carcinoma Adenoide Cístico/patologia , Adulto , Idoso , Neoplasias da Orelha/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/patologia , Neoplasias Nasais/patologia , Neoplasias da Glândula Submandibular/patologia
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