[Subunit immunogen of the tick-borne encephalitis virus. The isolation of glycoprotein V3 from 2 types of the tick-borne encephalitis virus and a study of its amino acid composition]. / Sub"edinichnyi immunogen virusa kleshchevogo éntsefalita. Vydelenie glikoproteida V3 iz dvukh tipov virusa kleshchevogo éntsefalita i izuchenie ego aminokislotnogo sostava.

An effective method for isolation of the membrane structural E protein, the so-called V3 glycoprotein, is described. Preliminary data of its amino acid composition have been obtained.

[Subunit immunogen of the tick-borne encephalitis virus. The immunological characteristics of glycoprotein V3 from 2 antigenic types of tick-borne encephalitis virus]. / Sub"edinichnyi immunogen virusa kleshchevogo éntsefalita. Immunologicheskie kharakteristiki glikoproteida V3 iz dvukh antigennykh tipov virusa kleshchevogo éntsefalita.

V3 glycoprotein isolated from virions of two antigenic types of TBE virus was found to be a valuable immunogen in experimental immunizations of rabbits and mice. The immune sera contained high titres of antibodies to TBE virus and other viruses of the TBE antigenic complex detectable in three serological tests. Species-, type-, and group-specific antibodies to TBE viruses were demonstrated. Mice vaccinated with TBE V3 glycoprotein developed immunity to TBE virus challenge. The isolated V3 glycoprotein of TBE virus, like whole TBE virus virions, carries several antigenic determinants.

[Cloning and primary structure of DNA copies of genome fragments of the tick-borne encephalitis virus]. / Klonirovanie i izuchenie pervichnoi struktury DNK-kopii uchastkov genoma virusa kleshchevogo éntsefalita.

RNA of a flavivirus-tick-borne encephalitis virus (Far-East, type 1, strain Sofin) was subjected to reverse transcription and the DNA copy was transformed into double-stranded DNA by action of E. coli DNA-polymerase (Klenow's fragment) without primer. The hairpin structures were removed by S1 nuclease. Oligo-dC ends were attached to ds-cDNA thus obtained, and this DNA was annealed with pBR322 plasmid cut by PstI and equipped with oligo-dG termini. The recombinant plasmids were cloned in E. coli HB101. Of the 360 TcrAps clones obtained, 187 clones efficiently hybridized with partially degraded 32P-RNA of TBE virus. The sequence of the insert of one of the clones was determined by the Maxam-Gilbert method. The 720 b.p. sequence is translatable into an amino acid sequence without interruption. Nearby the 3'-terminus of the insert, the sequence ACACAGG is present which is homologous with that found in RNA of the flavivirus West Nile.

[Clofibrate-induced changes in the content of a low molecular-weight polypeptide in rat liver cell cytoplasm]. / Izmenenie soderzhaniia nizkomolekuliarnogo polipeptida v tsitiplazme kletok pecheni krys pod vliianiem klofibrata.

Data from Na-DS polyacrylamide gel electrophoresis suggest that the relative amount of the polypeptide with molecular weight of 12,000 in the soluble fraction of rat liver cytosol is increased after treatment with the hypolipidemic agent clofibrate. The experimental results indicate that this polypeptide is identical to a fatty acid binding protein (Z-protein).