Cadmium inhibits GABA-activated ion currents by increasing intracellular calcium level in snail neurons.

Blocking of the GABA-activated chloride current by cadmium was investigated in identified nerve cells (RPeD1, RPaD1) of the pond snail (Lymnaea stagnalis L.), using a two-microelectrode voltage-clamp technique. Cd2+, at 50 microM extracellular concentration, inhibited GABA-activated chloride currents, both in normal and Ca2+-free solution. Intracellular injection of Ca2+ or the application of caffeine mimicked the inhibitory effect of Cd2+ on GABA-elicited currents. Cd2+-block was eliminated, or it was substantially decreased, when neurons were intracellularly loaded with EGTA, or when the Ca2+-release was blocked by ruthenium red. The blocking effect of Cd2+ was also eliminated by applying G-protein inhibitors: pertussis toxin, suramin or GTP-gamma-S. Finally, intracellularly injected Cd2+ was ineffective at eliciting an inward current on GABA-activated currents, suggesting that the Cd2+-binding site resides extracellularly. These results suggest that cadmium inhibited GABA-activated chloride currents by increasing the intracellular Ca2+ level, by releasing it from intracellular stores and by interacting with a putative G-protein-coupled cell-surface "metal-receptor".

Molluscs in biological monitoring of water quality.

Molluscs living in Lake Balaton accumulate persistent toxic substances, namely heavy metals, to a greater extent, than other organisms, and can serve as excellent passive biomonitors. Especially gills are good accumulators. Regular sampling showed that the level of Cd and Hg concentrations increased, while Pb contamination decreased during the past 20 years in mussels, corresponding probably to changes in pollution of the Lake. In functional, active monitoring various behavioral patterns of molluscs were employed. In mussels the periodicity of activity and rest (pumping activity vs. valve closure) is a sensitive indicator of unfavorable conditions and so of toxic substances. Low concentrations of inorganic and organic toxicants (heavy metals, PCBs, PAH compounds) cause reduction of the active and increase of the rest periods in a concentration dependent manner in a few hours. A second, suitable test for evaluating toxicity of chemicals is the measurement of the water flow through the exhalant siphon. Under the effect of toxicants the siphon activity, both the strength and duration of water flow changes characteristically within a few minutes. For both behavioral tests special techniques have been developed suitable for long duration recording, supported by mechano-electrical transduction and computerized data evaluation. In case of the pond snail (Lymnaea stagnalis L.) the behavior (positive/negative geotaxis and orientation) is disturbed in the presence of the above mentioned toxic chemicals. The execution and evaluation of the changes in the movement of the snail is based on video-recording and measurement of the direction and distance the animal performs in uncontaminated water and in the presence of the pollutants during the same period of time (0.5-4 h).

Age- and size-specific patterns of heavy metals in the organs of freshwater fish Abramis brama L. populating a low-contaminated site.

Concentrations of cadmium, copper, mercury, lead and zinc were determined by atomic absorption spectrophotometry in the muscle, gill and liver of bream Abramis brama L. to study the relationship between the heavy metal load of fish and their age and size, and the seasonal variation of pollutant loads. Fish were collected from the Western basin of Lake Balaton (Hungary) in October 1999 and May 2000. The average metal concentrations of different organs varied in the following ranges: Cd 0.42-2.10; Cu 1.77-56.2; Hg 0.01-0.19; Pb 0.44-3.24; Zn 10.9-82.5 microg g(-1) dry weight. The highest Cd, Cu, Pb and Zn concentrations were detected in the gill or liver of fish, whereas the highest Hg concentrations were measured in the muscle. In the liver of bream for cadmium, copper and mercury the Pearson correlation analysis revealed positive associations related to age and size (length, net weight), as well as for the mercury load of all three investigated organs. In the muscle and gill the copper, lead and zinc concentrations, similarly to the lead and zinc concentrations of the liver, the associations related to age and size were negative. The correlations between the heavy metal concentrations of organs and the individual condition factors of fish samples proved to have opposite trends compared to those related to the age and size of fish. The seasonal variations in the heavy metal load of bream could be attributed rather to the seasonal change in the condition factor of fish than to variations in the pollutant load of the site.