Facilitated remediation of heavy metals contaminated land using Quercus spp. with different strategies: Variations in amendments and experiment periods.

Phytoremediation using trees combined with soil amendments has gained much attention for its highly cost-effective trait. In natural field conditions, however, the results may not reflect the true performance of amendments based on short-term laboratory studies. In this three-year field trial, various soil amendments such as rice straw biochar, palygorskite, a combined biochar of rice straw biochar and palygorskite, and hydroxyapatite were used to systematically study the potential of the low-accumulator (Quercus fabri Hance) and high-accumulator (Quercus texana Buckley) for cadmium (Cd) and zinc (Zn) to remediate severely contaminated soils. Soil amendments enhanced the dendroremediation capacity of Quercus as the growth period prolonged. In 2021, the rice straw biochar treatment increased Cd and Zn accumulation by 1.76 and 2.09 times in Q. fabri, respectively, compared to the control. Cd and Zn accumulation increased to 1.78 and 2.10 times, respectively, under combined biochar treatment for Q. texana compared to the control. Metals accumulation was mainly enhanced by soil amendments through increasing the growth biomass of Q. fabri and improving the biomass and bioconcentration ability of Q. texana. Overall, soil amendments effectively improved the phytoremediation efficiency of Quercus in the long term, and selecting suitable amendments should be fully considered in phytoremediation.

Evaluation of dendroremediation potential of ten Quercus spp. for heavy metals contaminated soil: A three-year field trial.

Woody plants have gained considerable attention for remediating soils contaminated with heavy metals because of their cost-efficient and ecologically friendly nature. However, most studies on potential phytoremediation evaluation are limited to short-term experiments in greenhouse or field, meaning that differences may exist between laboratory results and application in natural environment. In this study, ten Quercus spp. were tested in a consecutive 3-year field trial (2018-2020) to assess their dendroremediation abilities for Cd and Zn contaminated soil. The results revealed that nine Quercus spp. demonstrated good survival ability without any stress, except for Quercus velutina Lam., in the 3-year growth period. In 2020, Quercus texana Buckley and Quercus fabri Hance plants produced the greatest biomass (2100 and 1880 g plant-1) among the nine Quercus spp. Quercus texana had the highest total Cd accumulation (39.3 mg plant-1) in 2020, which was 8.5 times higher than that in 2018, followed by Quercus pagoda Raf. (8.85 mg plant-1) and Q. fabri (8.07 mg plant-1) plants, respectively, whereas Cd accumulation increased by 7.4 times for Q. pagoda and 22 times for Q. fabri compared to 2018. The results from 2020 indicated that Q. fabri had the highest Zn accumulation (205 mg plant-1), followed by Quercus nigra L. (149 mg plant-1) and Q. texana (140 mg plant-1), respectively, and these values increased 14, 6.4, and 6.2 times in comparison to 2018. The comprehensive bioaccumulation index (CBAI) was proposed to evaluate the dendroremediation potential of Quercus spp., suggesting that Q. texana and Q. fabri had the most outstanding potential for remediation of Cd and Zn polluted soil, with the values of 0.82 and 0.60, respectively. In summary, Q. texana and Q. fabri are ideal for remediating Cd/Zn-contaminated soil, and long-term field trials and the CBAI method are helpful for comprehensively evaluating the remediation capacity of trees.

Effects of Contaminated Soil on the Survival and Growth Performance of European (Populus tremula L.) and Hybrid Aspen (Populus tremula L. × Populus tremuloides Michx.) Clones Based on Stand Density.

This study was conducted to assess the survival rates, growth, and chlorophyll fluorescence (Fv/Fm) of four hybrid aspen (14, 191, 27, 291) and two European aspen (R3 and R4) clones cultivated in creosote- and diesel oil-contaminated soil treatments under three different plant densities: one plant per pot (low density), two plants per pot (medium density), and six plants per pot (high density) over a period of two years and three months. Evaluating the survival, growth, and Fv/Fm values of different plants is a prerequisite for phytoremediation to remediate polluted soils for ecological restoration and soil health. The results revealed that contaminated soils affected all plants' survival rates and growth. However, plants grown in the creosote-contaminated soil displayed a 99% survival rate, whereas plants cultivated in the diesel-contaminated soil showed a 22−59% survival rate. Low plant density resulted in a higher survival rate and growth than in the other two density treatments. In contrast, the medium- and high-density treatments did not affect the plant survival rate and growth to a greater extent, particularly in contaminated soil treatments. The effects of clonal variation on the survival rate, growth, and Fv/Fm values were evident in all treatments. The results suggested that hybrid aspen clones 14 and 291, and European aspen clone R3 were suitable candidates for the phytoremediation experiment, as they demonstrated reasonable survival rates, growth, and Fv/Fm values across all treatments. A superior survival rate for clone 291, height and diameter growth, and stem dry biomass production for clone 14 were observed in all soil treatments. Overall, a reasonable survival rate (~75%) and Fv/Fm value (>0.75) for all plants in all treatments, indicating European aspen and hybrid aspen have considerable potential for phytoremediation experiments. As the experiment was set up for a limited period, this study deserves further research to verify the growth potential of different hybrid aspen and European aspen clones in different soil and density treatment for the effective phytoremediation process to remediate the contaminated soil.

Phytoextraction and recovery of rare earth elements using willow (Salix spp.).

Soil and water contaminations are caused by rare earth elements (REEs) due to mining and industrial activities, that threaten the ecosystem and human health. Therefore, phytoremediation methods need to be developed to overcome this problem. To date, little research has been conducted concerning the phytoremediation potential of Salix for REEs. In this study, two Salix species (Salix myrsinifolia and Salix schwerinii) and two Salix cultivars (Klara and Karin) were hydroponically exposed to different concentrations of six-REE for 4 weeks. The treatments were: T1 (Control: tap water), T2 (La: 50 mg/L) and T3 (La 11.50 + Y 11 + Nd 10.50 + Dy 10 + Ce 12 and Tb 11.50 in mg L-1). The effects of the REE on Salix growth indicators (height, biomass, shoot diameter and root length), concentrations of REE in the produced biomass, and accumulation of REE in different parts of the Salix (stem, root, and leaf) tissues, were determined. In addition, the retention of REE in ashes following Salix combustion (800 and 1000 °C) was determined. The result indicates that with La and REE exposure, the height growth, dry biomass, shoot diameter and root length of all Salix remained equivalent to the control treatment excluding Klara, which displayed relatively higher growth in all parameters. Further, among the REE studied, the highest La concentration (8404 µg g-1 DW) and La accumulation (10,548 µg plant-1) were observed in Karin and Klara root respectively. Translocations and bioconcentration factors were discovered at <1 for all Salix, which indicates their phytostabilization potential. The total REE concentrations in bottom ashes varied between 7 and 8% with retention rates between 85 and 89%. This study demonstrates that Salix are suitable candidates for REE phytostabilization and the remediation of wastewater sites to limit metals percolating to the water layers in the ecosystem.

The effect of particle size of bamboo biochar on the phytoremediation of Salix psammophila C. to multi-metal polluted soil.

Biochar shows great potential in soil remediation. The benefits of biochar on soil depend onits intrinsic properties and soil characteristics. However, the influence of particle sizes of biochar on soil remediation is not clear. In a pot experiment, we evaluated the effects of bamboo biochar (BBC) particle sizes (P1 < 0.15 mm, 0.15 mm < P2 < 0.25 mm, 0.25 mm < P3 < 0.50 mm) on phytoremediation efficiency of Salix psammophila C. cultivated in multi-metal polluted soil. We added the BBC at 3% (w/w) in tested soil. Next, the BBC was thoroughly mixed with soil and weighting to the pot, and S. psammophila cuttings were planted and grown for six months in the amended soil under model growth condition.Results revealed the addition of different sizes of BBC particles affected soil quality, plant growth, and HMs accumulation in plants. All sizes of BBC treatments improved Cd and Zn accumulation, whereas plants in P2 treatment showed the greatest accumulation, increased by 52.41 and 25.55% compared with the control (1,503 and 19,928 µg·plant-1). Overall, the results indicated BBC enhanced the phytoremediation efficiency of S. psammophila. Plants cultivated in P2 treatment showed the most significant effect on remediating contaminated soil.

Impacts of bamboo biochar on the phytoremediation potential of Salix psammophila grown in multi-metals contaminated soil.

We investigated the effects of bamboo biochar (BBC) as soil amendment on growth and phytoremediation potential of Salix psammophila in soil heavily polluted by Cd and Zn. Bamboo biochar was added to soil at ratios ranging from 1 to 7% (w/w), which significantly increased the organic matter, available potassium (K) content, while decreased the hydrolyzable nitrogen (N) content and the levels of total and bioavailable HMs in soil. The BBC amendment at ratios of 1% to 5% showed little effect on growth of plant, whereas at 7% ratio significantly decreased biomass compared to the control. BBC amendment stimulated the accumulation of Cu, Cd and Zn in plant tissues, meanwhile, Cd and Zn accumulation were more evident, especially in the BBC-3% treatment. BBC amendment improved the TF and BCF values of Cd, Zn and Cu compared to control. Higher BCF for Cd (BCF >1) and TF for Zn (TF >1) values indicate Salix psammophila have considerable potential for phytoremediation efficiency in BBC amended soil treatment. This study provides practical evidence of the efficient BBC-assisted phytoremediation capability of Salix psammophila and highlights its potential as a viable and inexpensive approach for in situ remediation.

Assessment of European and hybrid aspen clones efficiency based on height growth and removal percentage of petroleum hydrocarbons-a field trial.

Soils polluted by organic or inorganic pollutants are an emerging global environmental issue due to their toxic effects. A phytoremediation experiment was conducted to evaluate the extraction potential of three European aspen clones (R2, R3, and R4) and seven hybrid aspen clones (14, 27, 34, 134, 172, 191, and 291) grown in soils polluted with hydrocarbons (includes polycyclic aromatic hydrocarbons (PAH) and total petroleum hydrocarbons (TPH)). Height growth, plant survival rates, and .hydrocarbon removal efficiencies were investigated over a 4-year period at a site in Somerharju, Luumaki Finland, to assess the remediation potential of the clones. Hydrocarbon content in the soil was determined by gas chromatography and mass spectrometry. The results revealed that hybrid aspen clones 14 and 34 and European aspen clone R3 achieved greater height growth (171, 171, and 114 cm, respectively) than the other clones in the study. Further, the greatest removals of PAH (90% at depth 10-50 cm) and (86% at depth 5-10 cm) were observed in plot G15 planted with clone R2. Furthermore, the greatest TPH removal rate at 5-10 cm depth (C22-C40, 97%; C10-C40, 96%; and C10-C21, 90%) was observed in plot 117 with clone 134. However, other clones demonstrated an ability to grow in soils with elevated levels of TPH and PAH, which indicates their tolerance to hydrocarbons and their potential capacity for phytoremediation of hydrocarbon-polluted soils. Our study suggests that European aspen and hybrid aspen clones could be used for the remediation of soils polluted with PAH and TPH.

The simultaneous antibiotics and nitrogen removal in vertical flow constructed wetlands: Effects of substrates and responses of microbial functions.

A vertical flow constructed wetland (VFCW) packed with the different substrates was designed to remediate the antibiotics in the wastewater. Zeolite (CW-Z) paralleled with Manganese (Mn) ore (CW-M) and biochar (CW-C) were used to enhance the synchronous removal of ciprofloxacin hydrochloride (CIPH), sulfamethazine (SMZ) and nitrogen (N) from the wastewater. The result indicated that CW-M had a significant potential to remove CIPH (93%), SMZ (69%), TN (71%), NH4+-N (94%) and NO3--N (94%) across all the treatments. The abundance of amoA, nirK and nirS genes are dramatically higher in CW-M and CW-C, while CW-C inhibited the production of quinolone resistance genes. Results showed that different substrates could affect the microbial diversity and structure. The addition of Mn ore to the water led to an improved abundance of nitrogen-related phyla. Overall, Mn ore has a considerable potential to simultaneously remove antibiotics and N in VFCWs.

Phytoaccumulation of Zn, Pb, and Cd in Conocarpus lancifolius irrigated with wastewater: does physiological response influence heavy metal uptake?

Conocarpus lancifolius is a fast-growing and drought tolerant tree species with phytoremediation potential in arid environments. The present study was conducted to evaluate the phytoaccumulation potential under wastewater treatment. The experiment was performed in a greenhouse where 3-month-old seedlings were irrigated with industrial wastewater and growth, biomass and physiological parameters were measured. Concentrations of zinc (Zn), lead (Pb), and cadmium (Cd) in leaves, shoots, and roots along with translocation and tolerance index were also determined. The results showed that under wastewater treatment total biomass increased from 24.2 to 31.5 g, net CO2 assimilation rate increased from 9.93 to 13.3 µmol m-2 s-1, and water use efficiency increased from 1.7 to 2.42. Similarly, heavy metals (Zn, Pb, and Cd) accumulation in stem, leaves, and roots increased significantly under wastewater treatment where the highest concentration of Zn, Pb and Cd was found in roots followed by leaves and stem, respectively. Tolerance index was found >1, and translocation factor of all heavy metals was found >1. The study revealed that phytoaccumulation potential of C. lancifolius was mainly driven by improved net CO2 assimilation rate and water use efficiency.

Biomass growth variation and phytoextraction potential of four Salix varieties grown in contaminated soil amended with lime and wood ash.

In a greenhouse experiment, plant growth and copper (Cu) and zinc (Zn) uptake by four Salix cultivars grown in Cu and Zn contaminated soils collected from a mining area in Finland were tested to assess their suitability for phytoextraction. The cultivars displayed tolerance to heavily contaminated soils throughout the experiment. After uptake, total mean Cu concentrations in the leaves, shoots and roots in all cultivars and treatments ranged from 163 to 474 mg kg-1 and mean Zn concentrations ranged from 776 to 1823 mg kg-1. Lime and wood ash addition increased dry biomass growth (25-43%), chlorophyll fluorescence (Fv/Fm) values (3-6%), the translocation factor (TF) (15-60% for Cu; 10-25% for Zn), the bio-concentration factor (BCF) (40-85% for Cu; 70-120% for Zn), and metal uptake (55-70% for Cu; 50-65% for Zn) compared to unamended treatment across all cultivars. The results revealed that Salix cultivars have the potential to take up and accumulate significant amounts of Cu and Zn. Cultivar Klara (Salix viminalis × S. schwerinii × S. dasyclados) was found to be the most effective cultivar for phytoextraction since it displayed greater dry biomass production, Fv/Fm, TF, BCF values and uptake percentage rates of Cu and Zn compared to the other three cultivars. This study indicates that further research is needed to clarify the wider phytoextraction capabilities of different Salix cultivars.

Effects of soil amendments on the growth response and phytoextraction capability of a willow variety (S. viminalis × S. schwerinii × S. dasyclados) grown in contaminated soils.

This study was conducted to evaluate the effects of lime and bisphosphonates (BPs) such as N10O chelate amendment on the growth, physiological and biochemical parameters, and phytoextraction potential of the willow variety Klara (Salix viminalis × S. schwerinii × S. dasyclados) grown in soils heavily contaminated with copper (Cu), nickel (Ni) and zinc (Zn). The plants were irrigated with tap or processed water (mine wastewater). The results suggest that the combined effects of the contaminated soil and processed water inhibited growth parameters, gas exchange parameters and chlorophyll fluorescence (Fv/Fm) values. In contrast, malondialdehyde (MDA) content, organic acids, total phenolic and total flavonoid contents, and the accumulation of metals/metalloids in the plant tissues were increased compared to the control. When the soil was supplemented with lime and N10O; growth, physiological, biochemical parameters, and resistance capacity were significantly higher compared to unamended soil treatments, especially in the contaminated soil treatments. The combined lime‒ and N10O‒amended soil treatment produced higher growth rates, resistance capacity, photosynthesis rates and phytoextraction efficiency levels relative to either the lime‒amended or the N10O‒amended soil treatments. This study provides practical evidence of the efficient chelate‒assisted phytoextraction capability of Klara and highlights its potential as a viable and inexpensive novel approach for in situ remediation of Cu‒, Ni‒ and Zn‒contaminated soils and mine wastewaters.

Effects of contaminated soil on the growth performance of young Salix (Salix schwerinii E. L. Wolf) and the potential for phytoremediation of heavy metals.

Salix schwerinii was tested in a pot experiment to assess plant growth performance i.e., relative height and dry biomass and the potential for heavy metal uptake in soils polluted with chromium, zinc, copper, nickel and total petroleum hydrocarbons. The soil used in the pot experiment was collected from a landfill area in Finland. Peat soil was added at different quantities to the polluted soil to stimulate plant growth. The plants were irrigated with tap water or processed water (municipal waste water) to further investigate the effects of nutrient loading on plant biomass growth. The soil was treated at two pH levels (4 and 6). The results showed that the addition of 40-70% peat soil at pH 6 to a polluted soil, and irrigation with processed water accelerated plant growth and phytoextraction efficiency. In the pot experiment, Salix grown in chromium, zinc, copper, nickel and total petroleum hydrocarbons -contaminated field soil for 141 days were unaffected by the contaminated soil and took up excess nutrients from the soil and water. Total mean chromium concentration in the plant organs ranged from 17.05 to 250.45 mg kg-1, mean zinc concentration ranged from 142.32 to 1616.59 mg kg-1, mean copper concentration ranged from 12.11 to 223.74 mg kg-1 and mean nickel concentration ranged from 10.11 to 75.90 mg kg-1. Mean chromium concentration in the plant organs ranged from 46 to 94%, mean zinc concentration ranged from 44 to 76%, mean copper concentration ranged from 19 to 54% and mean nickel concentration ranged from 8 to 21% across all treatments. Under the different treatments, chromium was taken up by Salix in the largest quantities, followed by zinc, copper and nickel respectively. Salix also produced a moderate reduction in total petroleum total petroleum hydrocarbons in the polluted soil. The results from the pot experiment suggest that Salix schwerinii has the potential to accumulate significant amounts of chromium, zinc, copper and nickel. However, long term research is needed to verify the phytoextraction abilities of Salix observed in the pot experiment.