Sperm Quality in Mouse After Exposure to Low Doses of TCDD.

BACKGROUND: In the last decade, the harmful use of dioxin has been demonstrated in human health and in the whole environment. It is well known among scientists that 2, 3, 7, 8-tetrachloro dibenzo-p-dioxin (TCDD) is an environmental pollutant that causes endocrine disruption, which causes male reproductive toxicity. OBJECTIVE: The objective of the present study was to evaluate the toxicity effect of low doses of TCDD in male CD1 mice. MATERIALS AND METHODS: Three concentrations of TCDD (0.375, 0.75, 1.5 mg / kg) were analyzed and the effects on spermatozoa were evaluated 10 days after oral administration of the product. As bioindicators of TCDD toxicity, an exhaustive analysis of several spermatic parameters including motility, vitality, count, morphology and viability, flow cytometry was used to determine the affected sperm population by cytotoxicity and apoptosis. In addition, a morphometric analysis of testicles was performed. RESULTS: The results show that the body weight of the treated animals was reduced in medium and high doses (0.75, 1.5 mg / kg) with respect to the control groups. In the groups treated with TCDD, the abnormal head of the sperm increased by 52.5% more than the control group. Significant differences in apoptosis were observed between the negative control and vehicle control, including the median dose (0.75 mg / kg). CONCLUSION: It is concluded that at these low doses there was an impact on the quality of the mouse sperm, adding an effect on apoptosis and cytotoxicity of sperm exposed to these doses of TCDD.

Chemical composition and fatty acid profile in meat from grazing lamb diets supplemented with ryegrass hay, fishmeal and soya bean meal as PUFA sources / Perfil de ácidos graxos na carne ovina suplementado com farinha de peixe ou farinha de soja

ABSTRACT: This study examined the characteristics of the fatty acids profile in the meat of lambs fed grazing and supplemented with different PUFA sources. Thirty Suffolk lambs were used (37.2±5.4kg BW), grazing on perennial ryegrass (Lolium perenne) (12h d-1) and supplemented (30g kg-1 BW0.75) with ryegrass hay (RGH), or supplements formulated with fishmeal (FHM) or soybean meal (SBM). Lambs were used in a completely randomized design. Results showed an effect on total SFA, with SBM higher than RGH (P=0.033), and SBM and FHM showing higher MUFA content compared with RGH (P=0.001). Total PUFA content was higher for RGH, followed by FHM and with the lowest content for SBM (P=0.001). Concluding that there is a variation in fatty acid content depending on the lipid-protein source used in supplementation in grazing sheep, the content of PUFA was higher for grazing lambs supplemented with RGH compared with those supplemented with FHM or SBM.

RESUMO: Este estudo analisou as características dos ácidos graxos na carne de cordeiros alimentados com pastagem e suplementados com diferentes fontes de proteína. Trinta cordeiros Suffolk foram utilizados (37,2±5,4kg BW) que pastavam em azevém perene (Lolium perenne) (12h d-1) e suplementados com (30g kg-1 PV0,75) com azevém feno (RGH), ou concentrados formulado com farinha de peixe (FSM) ou farelo de soja (SBM). Os resultados mostram um efeito sobre o total SFA, sendo mais elevada do que SBM RGH (P=0,033). SBM e FSM mostrando o maior teor total de MUFA vs. RGH (P=0,001). O teor total de AGPI foi maior para RGH>EFM>SBM (P=0,001). Concluindo que há uma variação no teor de ácidos graxos dependendo da fonte de proteína utilizada na suplementação emanimais pastoreados, o teor de PUFA foi maior para ovinos mantidos em comparação com os suplementado com EFM ou SBM.