[Graft-versus-host reaction following granulocyte transfusion from a normal donor (author's transl)]. / Graft-versus-Host-Reaktion nach Granulozytentransfusion von einem Normalspender.

Graft-versus-Host disease following the transfusion of leukocytes from normal donor occured during remission-reinduction therapy in a 5 years old girl with acute lymphocytic leukemia. The clinical picture consisting of rash, diarrhea and somnolence was congruent with the diagnosis. Graft-versus-Host disease was confirmed by histology of skin biopsy and exclusion of other causes. The child survived the disease. We recommend the irradiation of leukocyte concentrates before transfusion.

[Problems of conductor demonstration in haemophilia A: error possibilities in the determination of factor-VIII-associated antigen]. / Zur Problematik des Konduktorinnen-Nachweises bei der Hämophilie A:Fehlermöglichkeiten bei der Bestimmung des Faktor VIII-assoziierten Antigens

Difficulties in the detection of haemophilia A carriers by the determination of the ratio factor VII coagulation activity to factor VII antigen concentration are shown in part one of our investigations. The second part evaluates methodical parameters of the quantitative immunoelectrophoretic technique. According to our results reliable data can only be obtained under the following conditions: 1. At least three determinations of each sample are required and the mean value should be used as the final result. 2. Each gel plate should be calibrated separately 3. The calibration line should include values less than 100%. Factor VIII concentrates are not suitable for determination of the calibration curve, as higer dilution results in lower calculated concentration of the undiluted concentrate. Storage of pooled normal plasma at--25 degrees C is possible without significant loss of antigen concentration over 5 months. However, plasma samples of a single person show an unpredictable variation in the antigen concentration during storage over the same time.

Studies on the specificities of various anti-H reagents.

Four different eel serum samples and the lectins Cytisus sessilifolius and Evonymus europaeus were examined for their capacities to react with a number of preparations which show O(H) blood group-like activity. Some similarities and major differences between the anti-H reagents were observed in experiments where the "H-like" activities of invertebrate extracts and blood group substances were compared. A comparison of the reactivities of eel sera, catfish anti-H sera and 4 anti-H lectins with a number of different "H-like" preparations is summarized. Data obtained from preliminary sugar analyses of preparations isolated from 5 different invertebrate species studied is presented.