Levels of luteinizing hormone (LH), follicle-stimulating hormone, and 17 beta-estradiol in response to D-Trp6-LH-releasing hormone during different phases of the menstrual cycle in normal women.

PIP: The response of serum LH, FSH, and 17beta-estradiol (17b-E) to stimulation with D-Tryp6-LH-releasing hormone (LH-RH analog) was measured in 34 normal women. The analog was administered either by vein, muscle, or continuous iv infusion in doses from 1 to 50 mcg during different phases of the menstrual cycle. The increase in LH and FSH to 10 mcg analog was significant (p .05) in all phases of the menstrual cycle by 60 minutes postadministration and lasted for at least 8 hours. 17b-E increased significantly by 8 hours. LH-RH analog was 40 and 21 times more potent than LH-RH in the stimulation of LH and FSH, respectively, during the follicular phase. In the ovulatory phase, LH and FSH responses differed kinetically from their responses in the other phases, and gonadotropin release was both faster and greater. The gonadotropin responses of 3 men when compared with those of the female subjects were almost always smaller (p .05). Because of the greater potency of this LH-RH analog, it may be useful in the treatment of infertility.^ieng

Serum LH, FSH and testosterone response to the administration of a new LH-RH analog, D-Trp6-LH-RH, in normal men.

A long-acting superactive analog of LH-RH, D-Trp6-LH-RH was given to 23 normal men by several routes of administration (iv, im, sc, continuous infusion) and in increasing doses of 1 to 50 microgram. LH and FSH responses were obtained at doses as low as 2.5 microgram. The maximal absolute LH and FSH increment in response to a 10 microgram iv bolus injection of this analog was similar to 100 microgram of LH-RH. In addition, after administration of the analog the LH and FSH level was maintained at a higher than basal level for at least 8 hours. With a 50 microgram iv bolus injection, from 30 minutes onwards the increases in LH levels were significantly greater (P less than 0.05) than those elicited by the 10 microgram dose for at least 8 hours. Maximum release of LH and RSH was observed when this same dose was given as continuous infusion for 8 hours (P less than 0.05). There seemed to be no significant differences between the im and sc routes. Following the administration of the D-Trp6-LH-RH, testosterone levels were maintained above the normal values (P less than 0.02). The high potency and prolonged duration of action of this compound suggest its potential usefulness for increasing testosterone levels and for stimulation of spermatogenesis in men.

[Clomiphen-test in anovulatory sterility (author's transl)]. / L'épreuve au clomiphène dans les stérilités par cycle anovulatoire

Twenty women with sterility due to an anovulatory cycle, were submitted to a load of hundred milligramms by day of clomiphene citrate during five days. The test was always performed between the 10th and the 14th day of the cycle. Before and after the clomiphene administration, FSH and LH in plasma and total estrogens and pregnanediol in 24 hours urine, were estimated. The results, are compared with those of ten normal women and the observed results may schematically been classified into three groups: A) Normal response. -- B) Week gonadotropic response. -- and C) High LH baseline. This results seem to indicate three main origines for the anovulatory cycle syndrome: hypothalamic, pituitary and ovarian.