RESUMO
Escherichia coli isolated from animals up to three months of age, with diarrhea (255 calves and 29 dogs (pups)), without diarrhea (21 calves and 11 pups, used as controls), and 58 adult dogs with cystitis were tested to investigate the occurrence and functional expression of cyclomodulins cycle inhibiting factor (CIF), cytotoxic necrotizing factors (CNFs) and cytolethal distending toxins (CDTs). In cyclomodulin-positive isolates the association was assessed with other virulence genotypes and phylogenetic groups. Of 374 E. coli isolates, 80 (21.4%) were positive for at least one cyclomodulin and 14 of the latter (3.7%) showed different combinations of more than one. cif-positive isolates showed a low number of additional virulence factors, and were commonly associated with phylogroup B1, while cnf- and cdt-positive isolates, harboring many extraintestinal virulence factors, belonged to phylogroups B2 and D. Almost all isolates showed an irreversible cytopathic effect (CPE), displaying functionality of cyclomodulins. Five isolates that presented a mutation of cif were CPE-negative.
Assuntos
Toxinas Bacterianas/metabolismo , Doenças dos Bovinos/microbiologia , Doenças do Cão/microbiologia , Proteínas de Escherichia coli/metabolismo , Escherichia coli/metabolismo , Regulação Bacteriana da Expressão Gênica/fisiologia , Animais , Toxinas Bacterianas/genética , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças do Cão/epidemiologia , Cães , Escherichia coli/genética , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Infecções por Escherichia coli/veterinária , Proteínas de Escherichia coli/genética , Itália/epidemiologia , Mutação , Filogenia , Fatores de Virulência/genética , Fatores de Virulência/metabolismoRESUMO
By means of a cell-counting technique, it has been shown that patients with Parkinson's disease underwent decreased density of striatal nerve cell population. Large cells were more severely affected than small cells. These findings support the view that striatal involvement, although not severe enough to be easily recognized by conventional neuropathological examination, might be responsible of the progressive loss of benefit that most patients experience during chronic L-dopa treatment.
Assuntos
Corpo Estriado/patologia , Neurônios/patologia , Doença de Parkinson/patologia , Idoso , Contagem de Células , Corpo Estriado/fisiopatologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Doença de Parkinson/fisiopatologiaRESUMO
A young male patient affected with Lafora's disease and concomitant mental deterioration, myoclonic jerks and epileptic seizures is reported. A cerebral biopsy showed round PAS-positive myoclonus bodies in nerve cells and neuropile. A tendency to periodic recurrence of paroxysmal activity in the EEG tracings, an unusual finding in Lafora's disease, is briefly discussed.
Assuntos
Córtex Cerebral/fisiopatologia , Epilepsias Mioclônicas/diagnóstico , Adolescente , Eletroencefalografia , Epilepsias Mioclônicas/patologia , Epilepsias Mioclônicas/fisiopatologia , Lobo Frontal/patologia , Humanos , Masculino , Periodicidade , RecidivaRESUMO
A significant correlation between age and decrease in the number of nerve cells has been found in the putamen. Both small and large cells in this nucleus were proportionally involved. A possible relationship with the outfall of cells in other structures of the brain, with which the putamen is connected, in order to balance the activity of the extrapyramidal system has been suggested.
