Effect of fermentation of chosen vegetables on the nutrient, mineral, and biocomponent profile in human and animal nutrition.

In the present study, the dry matter, crude ash, crude protein, ether extract, and energy, macro- (Na, K, Ca, Mg, P), micro- (Zn, Cu, Fe) minerals, heavy metals (Pb, Cd), vitamin C, A, carotene, and phenolic content were determined in chosen raw and fermented vegetables. The dietary intake of several macro- and microconstituents per one serving (100 g or humans and animals: ducks and pigs) was calculated. The fermentation process was found to reduce water and increase fat content in the vegetables. Lower levels of vitamin C and phenols were also found in the fermented vegetables. The vitamin A and carotene content in the fermented carrots and peppers were increased in comparison with the raw vegetables. The fermentation process decreased the concentration of some basic nutrients, mineral content, vitamins C and A, and phenols. Broccoli, peppers, and red beet had the highest levels of the analyzed nutrients and bioconstituents. The fermentation process is regarded by nutritionists as beneficial to human health. The addition of fermented plants is recommended in animal nutrition as well. This process modifies the chemical composition of preserved vegetables, e.g. it reduces the concentration of dietary fiber, and brings favorable effects in poultry and pig nutrition.

Effect of Raw Chickpea in the Broiler Chicken Diet on Intestinal Histomorphology and Intestinal Microbial Populations.

The aim of the study was to determine the effect of partial replacement of SBM protein with CPR-derived protein in the broiler rearing period from 22 to 42 days of age on the intestinal histomorphology and the composition of the intestinal microbial population. Male broiler chicks aged 1 day were assigned to two groups with different nutrition schemes (n = 100 in each, 5 cages of 20 chicks in each). The chickens were reared for 42 days. All birds were fed isonitrogenous and isoenergetic diets: starter (1 to 21 d), grower (22 to 35 d), and finisher (36 to 42 d). From rearing day 22, different diets were provided to the birds: the SBM group received feed with 100% soybean meal protein, and the diet of the CPR group the protein originated from soybean meal was replaced by 50% chickpea protein. The study results indicated a significant impact of the inclusion of CPR in the diet on the basic intestinal structures (thickness of myenteron: submucosa, jejunum and duodenum mucosa, and jejunum transversal lamina). The addition of CPR led to shortening of intestinal villi, an increase in villus thickness, and reduced intestine absorptive surface in the duodenum and jejunum. The CPR group exhibited a significantly lower villus length-to-crypt depth ratio in the jejunum (p < 0.001). The inclusion of chickpeas in the diet increased the total count of mesophilic bacteria and coliforms in the intestinal contents (p < 0.05). In summary, it has been demonstrated that the inclusion of CPR in the diet induced considerable disturbances in metabolism and intestinal structure. Although CPR is a cheap protein source, its use in poultry diet does not ensure development of the intestinal structure comparable to that in the case of an SBM-only diet.

Supplementation of Broiler Chicken Feed Mixtures with Micronised Oilseeds and the Effects on Nutrient Contents and Mineral Profiles of Meat and Some Organs, Carcass Composition Parameters, and Health Status.

In this study, we included 15% doses of infrared-irradiated camelina, flax, and sunflower seeds in the diets of broiler chickens (grower and finisher) and focused on assessing the effects on the production traits, selected slaughter analysis parameters, nutrients, and minerals in breast and drumstick meat and some organs, i.e., liver, proventriculus, and heart. In total, 200 one-day-old broiler chickens were assigned randomly to four treatments with five replicate cages of 10 broiler chickens per cage (five females and five males). The experiment lasted 6 weeks. In the group of broiler chickens in which diets were supplemented with micronised camelina and flax seeds, there was an increase in (p < 0.05) breast, thigh, and drumstick weight and a decrease (p < 0.05) in the abdominal fat of the carcasses. The oilseed treatments reduced the ether extract content and the calorific value in the breast and drumstick muscles and organs. The flax seeds contributed to an increase in the contents of Ca (breast muscle and liver), Cu (breast muscle and proventriculus), and Fe (drumstick muscle and heart). Likewise, some blood parameters were influenced by supplementation with infrared-irradiated camelina and flax seeds, for example, there was a decrease in the haemoglobin level and the mean corpuscular haemoglobin concentration (p < 0.05). The oilseed treatments also modified the contents of Fe and Ca in the blood plasma of broiler chickens (p < 0.05). It may be concluded that infrared-irradiated camelina, flax, and sunflower seeds can be regarded as good diet components exerting positive effects on the dietary value of poultry meat and organs used in dietetics.

Effect of Microbial Phytase on Ileal Digestibility of Minerals, Plasma and Urine Metabolites, and Bone Mineral Concentrations in Growing-Finishing Pigs.

The study was conducted to evaluate the effects of added phytase in the diet of pigs on utilization of minerals and to determine the essential levels of this supplement in compound feed. An additional aim of the study was a critical assessment of current recommendations regarding the level of phosphorus in the diet of pigs, taking into account the use of phytase. A total of 432 pigs were allotted to six dietary treatments, with nine replicate pens per treatment according to body weight (BW) and sex. The treatments included a negative control (NC) with reduced content of digestible phosphorus; the NC diet supplemented with 6-phytase produced by a genetically modified strain of Aspergillus oryzae in the amount of 250 (NC + 250), 500 (NC + 500), 1000 (NC + 1000), or 1500 (NC + 1500) FTU/kg of feed; and a positive control (PC) diet formulated to meet NCR nutrient requirements for pigs. The results showed that, the higher the phytase activity in the diet (NC+), the lower the concentration of P, Ca, and Mg in the chyme (p < 0.05). Pigs fed the PC and NC+ diets had higher plasma levels of P and Ca than group NC in both fattening periods (p < 0.05). The content of phosphorus in the femur of pigs increased with the level of phytase added to the diet (p < 0.05). The content of Cu and Zn in the femur of pigs in the growing period was higher in groups NC + 500 and NC + 1000 than in the remaining experimental groups (p < 0.001). The content of P and Ca in the feces was higher in pigs fed the PC diet in comparison to the remaining experimental groups in both fattening periods (p < 0.001). There was a decrease in the content of P, Ca, and Mg in the excreta of pigs fed NC+ diets in both fattening periods (p < 0.05). A linear decrease in excretion of zinc in the feces was noted in the case of high levels of phytase, i.e., 1000 and 1500 FTU (p < 0.001). Increasing the level of phytase decreased the Cu (p < 0.001) content in the urine of growing−finishing pigs. In conclusion, the analysis of the effects of the use of phytase in a range of 0−1500 FTU/kg in low-phosphorus diets for fattening pigs indicates that 1000 FTU is the most effective level of phytase for increasing utilization of minerals and reducing excretion of elements into the environment.

Effect of inclusion of micronized camelina, sunflower, and flax seeds in the broiler chicken diet on performance productivity, nutrient utilization, and intestinal microbial populations.

The aim of the study was to evaluate the effect of inclusion of micronized full-fat camelina, flax, or sunflower seeds in the diet for broiler chickens on the performance productivity, nutrient utilization, and composition of intestinal microbial populations and to assess the possibility of modification of the resistance of isolated bacteria to chemotherapeutic agents with different mechanisms of action. The use of micronized oilseeds improved the broiler chicken body weight (P = 0.035) and the FCR value (P = 0.045) in the final rearing stage by enhancement of the utilization of total protein and organic matter. Lactobacillus-Enterococcus spp., Bifidobacterium spp., Escherichia coli, and Salmonella spp. were isolated from small intestinal contents, and Enterobacteriaceae taxa were detected in the cecum and cloaca of the broiler chickens. The addition of micronized camelina seeds (CAM.IR) contributed to an increase in the Bifidobacterium counts in the small intestine, compared with the control treatment (P < 0.050). Escherichia coli bacteria were not isolated only in the CAM.IR treatment. Nitrofurantoin and chloramphenicol were the most effective agents against the isolates from the cecum and cloaca in all oilseed treatments, whereas streptomycin exhibited the lowest efficacy. In the CAM.IR and micronized sunflower seed (SUN.IR) treatments, there were higher counts of trimethoprim/sulfamethoxazole-resistant Enterobacteriaceae strains than in the control and micronized flax seed (FLA.IR) treatments (P < 0.05). There was a difference between strains isolated from the cecum and cloaca only in the FLA.IR treatment, i.e., increased tetracycline sensitivity was exhibited by strains isolated from the cloaca (13% vs. 50%), also in comparison with the control treatments (P = 0.054). In comparison with the CAM.IR and control treatments, reduced numbers of multi-resistant strains were found in the cloaca isolates from the for FLA.IR and SUN.IR variants. Micronized camelina, flax, and sunflower seeds can be used as part of an effective nutritional strategy focused on optimization of the efficiency of rearing broiler chickens, as they positively modify intestinal microbial populations and increase bacterial sensitivity to the analyzed chemotherapeutic agents.

Nutritional and Anti-Nutritional Factors in Vicia sativa L. Seeds and the Variability of Phenotypic and Morphological Characteristics of Some Vetch Accessions Cultivated in European Countries.

Agricultural research has traditionally focused on staple crops, while relatively little attention has been given to minor crops. Therefore, this study aimed to determine the nutrient contents and some anti-nutritional factors in the seeds of common vetch (Vicia sativa L.) and the variation of phenotypic and morphological traits in field studies of 44 European accessions, originating from Russia, Ukraine, Poland (east and east-central Europe), the Czech Republic, the Slovak Republic, Hungary (south-central Europe), and Germany (west-central Europe) and grown in the same soil-climate conditions. A three-year field study was conducted from 2010 to 2012. Accessions originating from west-central Europe flowered three days earlier than those from south-central Europe. They also had the lowest seed number per pod (5.9) but the highest thousand seed weight (58 g) (p < 0.05). Vetch lines coming from south-central Europe contained the highest level of crude protein in comparison with vetch seeds originating from west-central Europe (353 vs. 324 g kg-1, respectively) (p < 0.05), but the latter had the highest essential amino acid index value (75 vs. 71 in west-central Europe and south-central Europe, respectively) (p < 0.05). The highest protein level was noted in the seeds of Slovak origin (average 358 g kg-1), whereas the lowest protein level (324 g kg-1) was determined in the Russian and German lines. Vetch grain fat was rich in linoleic (53%) and linolenic (14%) acids. The best n-6/n-3 fatty acid ratio (4) and the highest α-linolenic acid level were exhibited by the Slovak and Polish accessions (p < 0.05). The seeds of vetch accessions from east, east-central, and south-central Europe contained higher levels of (p < 0.05) iron than those originating from west-central Europe. The concentration of tannins, trypsin inhibitors, and hydrogen cyanide reached on average 8, 3, and 81 mg kg-1, respectively. The highest hydrogen cyanide (HCN) levels was determined in the accessions of German and Russian origin (126 and 119 mg kg-1, respectively), and the lowest values were noted in the Slovak and Polish lines (50 and 67 mg HCN kg-1, respectively). Furthermore, the Polish accessions contained the lowest levels of tannins and trypsin inhibitors. Common vetch seeds may be valuable protein feed/food stuff, but their nutritional usefulness is limited by the presence of anti-nutritional factors, especially HCN, which is difficult to remove. Further selection in this direction may be postulated.

Inclusion of Camelina, Flax, and Sunflower Seeds in the Diets for Broiler Chickens: Apparent Digestibility of Nutrients, Growth Performance, Health Status, and Carcass and Meat Quality Traits.

The study determined the effect of the addition of 15% of camelina, flax, and sunflower seeds to iso-caloric and iso-nitrogenous diets for broiler chickens during 21-42 days of age on the nutrient digestibility, production traits, slaughter analysis parameters, hematological indices, blood mineral elements, and dietary value of breast and drumstick meat. Two hundred one-day-old broiler chickens were assigned to four groups (treatments) with five replicates (10 birds per cage, 5 females and 5 males). The experiment lasted 6 weeks. Broiler chickens receiving diets supplemented with camelina and flax seeds exhibited an increase (p < 0.05) in average body weight and a decrease (p < 0.05) in the ether extract content and energy digestibility of the diets. Moreover, the best carcass quality with a high proportion of muscles and low abdominal fat content (p < 0.05) was noted in broilers fed flax- and sunflower-enriched diets. The treatments with the oil seeds reduced the ether extract content and the calorific value of breast and drumstick muscles. The flax seeds contributed to an increase in the Fe content in drumstick muscles. Additionally, some blood parameters were influenced by the flax seed supplementation, e.g., the level of hemoglobin declined (p < 0.05) and the iron level in plasma increased (p < 0.05). It can be concluded that the camelina, flax, and sunflower seeds can be regarded as good dietary components with positive effects on the dietary value of poultry meat.

Immunomodulant feed supplement Boswellia serrata to support broiler chickens' health and dietary and technological meat quality.

Supplementation of broiler chicken diets with resin rich in bioactive components, such as different boswellic acids, can improve the productivity, fatty acid composition, and technological parameters of produced meat. The aim of the study was to assess the effect of different levels of Boswellia serrata resin (BSR) supplementation in broiler chicken diet on fatty acid profiles in tissues and meat quality (physicochemical properties). The experimental Ross 308 broiler chickens were randomly assigned to 4 dietary treatments with 5 cages per treatment. The dietary treatments applied for 6 wk consisted of the control (C) and supplementation with 1.5 (BSR1.5), 2 (BSR2), or 2.5% (BSR2.5) of BSR resin. All the diets were isoenergetic and isonitrogenous. The BSR supplementation had a positive effect (P < 0.05) on the share of polyunsaturated fatty acid in the sum of total fatty acids in breast and drumstick muscles and abdominal fat. In addition, the following dietary parameters of the meat were improved: n-3/n-6, saturation, atherogenic and thrombogenic indices, and hypocholesterolemic/hypercholesterolemic ratio. The addition of BSR to the broiler chicken diets increased linearly (C vs. BSR, P < 0.05) the physicochemical properties of the breast and drumstick muscles: water-holding capacity and cooking losses. The color parameter a* decreased linearly (P = 0.033) in the breast muscles of the BSR-treated broiler chickens (8.6 and 7.8% C vs. BSR2 and BSR 2.5).

Comparative analysis of selected bioactive components (fatty acids, tocopherols, xanthophyll, lycopene, phenols) and basic nutrients in raw and thermally processed camelina, sunflower, and flax seeds (Camelina sativa L. Crantz, Helianthus L., and Linum L.).

The aim of study was to determine the content of basic nutrients, the level of fatty acids, tocopherols, xanthophyll, and lycopene, and the total phenolic content in camelina (Camelina sativa L. Crantz) (Cs), sunflower (Helianthus L.) (Ha), and flax (Linum L.) (Lu) seeds. The seeds were either raw or subjected to processing, i.e. boiling, micronization, or microwave roasting. The basic chemical composition was established and the fatty acid composition as well as the content of tocopherol (α, ß, γ, δ, total), ß-carotenoids, xanthophyll, lycopene, and total phenolics were determined in the analyzed oil seeds. The analyzed oil seeds are a rich source of protein and PUFAs as well as α-tocopherols (Ha) and γ-tocopherols (Cs, Lu), xanthophyll, and phenolics One portion of seeds covered from 746/513 (Cs) to as much as 1209/813% (Lu) (female/male) of the ALA daily intake. The AI value in the processed seeds increased (P < 0.05) and the values of H/H and HC declined (P < 0.05). The oil seed processing resulted in loss of most nutrients and bioactive constituents and appearance of some amounts of trans isomers, especially in the microwave roasted seeds (0.99-1.79 g/100 g crude lipid). The phenolic content decreased in the boiled seeds (Ha: 1301; Cs: 578.3, and Lu: 62.75 mg/100 g).

Nutrient and Mineral Profile of Chosen Fresh and Smoked Fish.

In the present study, were determined the basic nutrients (dry matter, crude ash, crude protein, ether extract, and energy) and mineral elements content in chosen species of raw and smoked freshwater and sea fish. The content of dry matter, and basic nutrients and Na+, K+, Ca+2, Mg+2, P+2, Zn+2, and Cu+2 in the fish samples was determined. The dietary intake of several macro- and microconstituents per one serving (150 g fresh or smoked fish) was calculated. The fresh fish contained on average 220.2 to 283.7 g·kg-1 of dry matter, 12.4 to 10.7 g·kg-1 of crude ash, 176.2 to 173.5 g·kg-1 of crude protein, 32.6 to 78.6 g·kg-1 of ether extract, and 104.6 to 119.1 kcal (freshwater and sea fish, respectively). Thermal treatment reduces the water and fat content in fish meat. Reduction of the K, Ca, Mg, P, Zn, and Cu levels was observed most frequently. The one serving of fish covers approximately 23% and 12% of the recommended dietary amount of K, 7.5-5.0% of Ca, ~12% of Mg, 6.8 to 12.5% of Zn, and about covered 6.7% of Cu. The smoking process increased the concentration of some basic nutrients and reduced the fat and mineral content. Whitefish, trout, halibut, mackerel, and herring had the highest levels of the analyzed minerals.

Peroxidation and unsaturation coefficients as biomarkers of environmental micro-exposure to molluscicides in Helix pomatia L.

This study was a follow-up of a previous study that investigated a set of selected fatty acids (FAs; 12 of 56 pools) in Helix pomatia L. as biomarkers of chemical stress induced by applying micro-doses of molluscicides. Here, the potential of rarely used peroxidation (PI) and unsaturation (UI) coefficients were tested as biomarkers. These indices were calculated based on the FA profiles of foot and hepatopancreas tissues of H. pomatia L. Snails were treated with three molluscicides: metaldehyde, methiocarb, and potassium chloride, in three doses each (5, 10, or 15 µl, 0.01% w/v concentration), for 12 weeks, under laboratory conditions. Correlations were evaluated between frequently used oxidation status indicators (catalase, glutathione peroxidase, selenium-dependent peroxidase, superoxide dismutase, glutathione transferase, glutathione reductase, glutathione, carbonyl protein, and thiobarbituric acid reactive substances, in the form of MDA) and UI and PI ratios. These results confirmed that fatty acids could be directly used as biomarkers of exposure and oxidative physiological status in snails. Moreover, the UI and PI, calculated based on FAs, clearly reflected the current oxidation status in snails. These indices changed with the application of micro-doses of molluscicides. In conclusion, these indices could serve as sensitive biomarkers of chemical stress in snails.

Higher Ca and Na content in the hair of obese people in Poland.

The authors of this paper decided to check whether the content of selected minerals in human hair is projected into the nutritional status of the body expressed as the BMI. The study focused on evaluating the content of calcium, sodium, potassium and magnesium in the hair of 7845 individuals in relation to their BMI. 5126 women and 2719 men aged from 18 to 92, domiciled throughout Poland, were involved in the study. Hair samples were taken from several points of the occipital scalp. Ca, Na, K and Mg in the hair were determined using inductively coupled plasma optical emission spectrometry (ICP-OES). The content of Ca and Na in the hair of obese people (>30.0 kg·m2) was higher (323 mg·kg-1 and 180 mg·kg-1) in comparison with the groups of people with normal body weight and those grade 2 underweight (<16.9 kg·m2): 191 mg·kg-1 and 103 mg·kg-1 respectively. The highest level of K (317 mg·kg-1) was found in the hair of people classed as grade 2 underweight. The body mass index caused no differentiation in the content of Mg in hair. Positive correlations were found between Ca-Na, Ca-Mg and Na-Mg and between the BMI of the subjects and the content of Ca in hair (R = 0.163; p < 0.01) and between the BMI and the content of Na (R = 0.191; p < 0.01). On the other hand, a negative relation between K and the BMI was correlated only to an infinitesimal degree (R = -0.030, p < 0.01). The results point to a relationship between the body mass index and the content of Ca, Na and K in the hair of adults.

Dietary Intake and Content of Cu, Mn, Fe, and Zn in Selected Cereal Products Marketed in Poland.

This paper aims to verify whether cereal products are a good source of Cu, Mn, Fe, and Zn in the diets of Poles. The study material comprised of 445 cereal products including baked goods, breakfast cereals and groats, pasta, and rice. Products that required culinary treatment (pasta, groats, rice) were boiled in drinking quality water as recommended by the producer. The content of Cu, Mn, Fe, and Zn was determined by using the FAAS method. The average content of microelements in the analyzed products can be represented as Fe (17.9 mg kg-1 ± 10.3) > Zn (12.4 mg kg-1 ± 5.2) > Mn (9.6 mg kg-1 ± 6.7) > Cu (3.9 mg kg-1 ± 2.9). Considering the daily requirement of the analyzed minerals among adult Poles, it was determined that cereal products supply 58% RDA Cu, 61% AI Mn (men)-78% AI Mn (women), 19% RDA Fe (women)-34% RDA Fe (men), and 16% RDA Zn (men)-22% RDA Fe (women). Baked goods account for as much as about 90% of all Cu, Mn, Fe, and Zn consumed as cereal products in a daily diet. Based on the results, it can be concluded that cereal products are the main source of Cu, Mn, Fe, and Zn in the diets of Poles. In connection with low assimilability of minerals, they should not be considered the fundamental source of those microelements in the diets of Poles.

Comparative effect of different dietary inulin sources and probiotics on growth performance and blood characteristics in growing-finishing pigs.

The study was focused on assessment of the effect of an extract of long-chain inulin (LCI) and dried tubers of Jerusalem artichoke (JA) and a multispecies probiotic preparation as well as a combination thereof on growth performance and blood parameters of fattening pigs. In total, 144 pigs (initial body weight 30.0 ± 0.5 kg) were used in a 98-d experiment. The six dietary treatments consisted of the control diet (Con), diet Con supplemented with probiotics (ConP) and four diets supplemented with LCI or JA alone or with probiotics (diets LCIP and JAP). Throughout the fattening period, there was a beneficial effect of the probiotic supplementation to the inulin-containing diets and the average daily gain (ADG) was increased by supplementation of probiotics in combination with inulin sources (p < 0.05). At the end of the fattening period, ADG and feed conversion ratio (FCR) were higher after supplementation of LCI only (p < 0.05). Compared with group ConP, in groups LCI and JA, the ADG and FCR were improved (p < 0.05). Only in the first fattening stage, the addition of the prebiotics and/or probiotics had an impact on the level of white blood cells and some biochemical indices in pigs. In younger animals, probiotic or LCI supplementation increased the IgG level (p < 0.05). There was also an interaction between the probiotics and JA resulting in increased IgG and IgA concentrations (p < 0.05). In the finishing period, LCI addition increased the IgM level (p < 0.05), whereas JA addition increased IgG and IgM levels as well (p < 0.05). In conclusion, both dietary sources of inulin and probiotic supplementation can improve the fattening performance and health status of growing pigs.

Content of Minerals and Fatty Acids and Their Correlation with Phytochemical Compounds and Antioxidant Activity of Leguminous Seeds.

The aim of the study was to determine the mineral composition and fatty acid profile in the seeds of selected Fabaceae species and cultivars and to assess their correlations with phytochemicals and antioxidant activity. The Andean lupine was characterised by a particularly high level of Mg and K as well as Cu, Zn, and Fe (P < 0.05). There were various correlations (P < 0.05) between the total phenols and tannins and these elements. The highest contribution of α-linolenic acid (ALA, 18:3, n-3) in total fatty acids was noted in the lentil (13.8 in 100 g-1 fat), common bean (11.9 in 100 g-1 fat), and pea seeds (10.4 in 100 g-1 fat) (P = 0.028). In turn, the white lupine contained the highest content of ALA-0.67 g 100 g-1 seeds; its lowest level was determined in the broad bean-0.03 g 100 g-1 seeds. The seeds exhibited a high proportion of hypocholesterolemic fatty acids (on average 86%). The 2,2-diphenyl-1-picrylhydrazyl antiradical activity was positively correlated with UFA and PUFA (P < 0.05). This indicates great protective potential of legume seeds for prevention and treatment of diet-dependent diseases.

Determination of Minerals in Herbal Infusions Promoting Weight Loss.

The study aimed at determination of the mineral composition of slimming herbal teas and estimation of the coverage of their total intake with infusions in women's daily diet. The content of Na+, K+, Ca+2, Mg+2, Zn+2, Cu+2, Fe+2, and Mn+2 was determined in infusions and mineralisates obtained from the slimming herbal teas. Among macroelements, the highest content was recorded for Ca-on average 3.73 mg·100 ml-1 in its infusion. Mn was a microelement with the highest concentration amounting to 0.20 mg·100 ml-1 in the infusion. The investigations revealed that, referring to the dietary reference intakes (DRIs), weight loss herbal infusions cover the recommended daily intake of manganese for women to the highest extent (on average 54 %), which suggests that they can be a major source of this microelement for the organism. Herbal teas only to a slight extent (to approx. 4 %) covered the recommended daily intake of magnesium, sodium, potassium, iron, zinc, copper, and calcium in the daily diet.

Effect of micronized pea seeds (Pisum sativum L.) as a substitute of soybean meal on tissue fatty acid composition and quality of broiler chicken meat.

This study aimed to evaluate the effect of micronized pea seeds introduced into feed mixes for broilers on the slaughter yield, blood lipid parameters, content of fatty acids in selected tissues, and meat quality. The studies involved 150 1-day-old Ross 308 chicks split into three groups (for 42 days). The feed rations differed in terms of the source of proteins: in the control group (C), it was post-extraction soybean meal (SBM) 100%; in group I, SBM 50% and micronized peas 50%; and in group II, micronized peas only, 100%. Irradiated pea seeds added to the feed ration for chicks reduced the fattening grade of carcasses (P < 0.05). Additionally, significant improvement of blood lipid indices was recorded. The share of the irradiated pea seeds in feed mixes decreased the share of saturated fatty acids in the muscles and abdominal fat and had a positive effect on the n-6/n-3 ratio, hypocholesterolemic / hypercholesterolemic ratio, as well as the atherogenic and thrombogenic indices (P < 0.05).

Content of Selected Minerals and Active Ingredients in Teas Containing Yerba Mate and Rooibos.

The study aimed to determine the content of selected elements: sodium, potassium, copper, zinc, iron, manganese and active ingredients such as phenolic acids and tannins in teas containing Yerba Mate and Rooibos cultivated in various areas. The study material comprised six samples of Yerba Mate teas and of Rooibos teas, both tea bags and leaves, purchased in Pulawy and online via Allegro. In total, 24 samples were tested. Yerba Mate was particularly abundant in Mn and Fe. The richest source of these elements was Yerba Mate Yer-Vita (2261.3 mg · kg(-1) d.m.) and (691.6 mg · kg(-1) d.m.). The highest content of zinc was determined in Yerba Mate Amanda with lime (106.0 mg · kg(-1) d.m.), while copper was most abundant in Yerba Mate Big-Active cocoa and vanilla (14.05 mg · kg(-1) d.m.). In Rooibos, the content of sodium was several times higher than in Yerba Mate. A clear difference was observed in the content of minerals in dry weight of the examined products, which could be a result of both the taxonomic distinctness and the origin of the raw material. Leaf teas turned out to be a better source of tannins; on the other hand, tea bags contained substantially more phenolic acids. The richest source of phenolic acids was Yer-Vita in bags (1.8 %), and the highest amount of tannins was recorded in the leaf tea Green Goucho caramel and dark chocolate (9.04 g · 100 g(-1) d.m.). In Rooibos products, the highest content of phenolic acids was recorded in tea bags (Savannah with honey and vanilla 0.96 %), and tannins in (Lord Nelson with strawberry and cream 7.99 g · 100 g (-1) d.m.).