Wickerhamiella lachancei f.a. sp. nov., a novel ascomycetous yeast species isolated from flowers of Lantana camara in India.

Two isolates representing a novel species of the genus Wickerhamiella were obtained in India from nectar of flowers of Lantana camara, an ornamental exotic species native to Central and South America. Phylogenetic analyses of the D1/D2 domain of the 26S large subunit (LSU) rRNA gene, internal transcribed spacer (ITS) region, and physiological characteristics, supported the recognition of the novel species, that we designate Wickerhamiella lachancei sp. nov (MycoBank no. MB851709), with MCC 9929T as the holotype and PYCC 10003T as the isotype. Considering pairwise sequence similarity, the type strain of the novel species differs from the type strain of the most closely related species, Wickerhamiella drosophilae CBS 8459T, by 16 nucleotide substitutions and two gaps (3.9 % sequence variation) in the D1/D2 region (560 bp compared) and 28 nucleotide substitutions and five gaps (7.22 % sequence variation) in the ITS region (444 bp compared).

Extensive remodeling of sugar metabolism through gene loss and horizontal gene transfer in a eukaryotic lineage.

BACKGROUND: In yeasts belonging to the subphylum Saccharomycotina, genes encoding components of the main metabolic pathways, like alcoholic fermentation, are usually conserved. However, in fructophilic species belonging to the floral Wickerhamiella and Starmerella genera (W/S clade), alcoholic fermentation was uniquely shaped by events of gene loss and horizontal gene transfer (HGT). RESULTS: Because HGT and gene losses were first identified when only eight W/S-clade genomes were available, we collected publicly available genome data and sequenced the genomes of 36 additional species. A total of 63 genomes, representing most of the species described in the clade, were included in the analyses. Firstly, we inferred the phylogenomic tree of the clade and inspected the genomes for the presence of HGT-derived genes involved in fructophily and alcoholic fermentation. We predicted nine independent HGT events and several instances of secondary loss pertaining to both pathways. To investigate the possible links between gene loss and acquisition events and evolution of sugar metabolism, we conducted phenotypic characterization of 42 W/S-clade species including estimates of sugar consumption rates and fermentation byproduct formation. In some instances, the reconciliation of genotypes and phenotypes yielded unexpected results, such as the discovery of fructophily in the absence of the cornerstone gene (FFZ1) and robust alcoholic fermentation in the absence of the respective canonical pathway. CONCLUSIONS: These observations suggest that reinstatement of alcoholic fermentation in the W/S clade triggered a surge of innovation that goes beyond the utilization of xenologous enzymes, with fructose metabolism playing a key role.

Genomic factors shape carbon and nitrogen metabolic niche breadth across Saccharomycotina yeasts.

Organisms exhibit extensive variation in ecological niche breadth, from very narrow (specialists) to very broad (generalists). Two general paradigms have been proposed to explain this variation: (i) trade-offs between performance efficiency and breadth and (ii) the joint influence of extrinsic (environmental) and intrinsic (genomic) factors. We assembled genomic, metabolic, and ecological data from nearly all known species of the ancient fungal subphylum Saccharomycotina (1154 yeast strains from 1051 species), grown in 24 different environmental conditions, to examine niche breadth evolution. We found that large differences in the breadth of carbon utilization traits between yeasts stem from intrinsic differences in genes encoding specific metabolic pathways, but we found limited evidence for trade-offs. These comprehensive data argue that intrinsic factors shape niche breadth variation in microbes.

Phylogenomic delineation of two new species of ascomycetous yeasts, Wickerhamiella koratensis sp. nov. and Wickerhamiella limtongiae sp. nov., and proposal of two synonyms, Wickerhamiella infanticola and Wickerhamiella tropicalis.

Two novel ascomycetous yeast species of the genus Wickerhamiella are proposed based on isolates obtained in Thailand from food waste and the fruiting body of a polypore fungus, and on a combination of conventional DNA-barcode sequence analyses and whole-genome phylogenies. We focus on a particular subclade of the genus Wickerhamiella that contains species found in anthropic environments and describe Wickerhamiella limtongiae sp. nov. (DMKU-FW31-5T=PYCC 9022T=TBRC 15055T), found on food waste samples. In an adjacent clade, we describe Wickerhamiella koratensis sp. nov. (DMKU-KO16T=PYCC 8908T=TBRC 14869T), which represents the closest relative of Wickerhamiella slavikovae and was isolated from the fruiting body of Bjerkandera sp. In the subclade of W. limtongiae sp. nov., we propose that Wickerhamiella infanticola should be regarded as a synonym of Wickerhamiella sorbophila and that Wickerhamiella tropicalis should be regarded as a synonym of Wickerhamiella verensis.

Comparative Efficacy of iPACK vs Popliteal Sciatic Nerve Block for Pain Management Following Total Knee Arthroplasty: A Retrospective Analysis.

Introduction Total knee arthroplasty (TKA) is associated with severe acute postoperative pain. The use of tourniquets and drains (T/D) is common in TKA but may have an influence on postoperative pain and muscular strength. The infiltration of local anesthetic between the popliteal artery and capsule of the knee (iPACK block) is a motor-sparing block that provides analgesia to the posterior aspect of the knee. However, evidence regarding its efficacy is scarce. This study aims to assess the effectiveness of iPACK block and the impact of T/D use on pain and muscular strength after TKA. Material and methods A retrospective study was carried out including patients who underwent TKA from January 2020 to April 2023. Patients were allocated into groups according to the peripheral nerve block performed and T/D use. Results We included 415 patients in this study. No differences were found in pain at rest or the need for rescue analgesia between patients who received an iPACK block or sciatic nerve block (SNB) with T/D applied. Patients who received a SNB reported lower pain scores on movement (p = 0.019), but with a higher prevalence of motor block (p < 0.001). Patients who underwent surgery without using T/D reported lower pain scores on movement (p = 0.021) and reduced need for rescue analgesia (p = 0.041). Conclusion These findings indicate that iPACK block can facilitate early mobilization after TKA without significant impact on postoperative muscle strength. Furthermore, the use of a T/D may be a source of postoperative pain that could compromise early rehabilitation.

Tracking alternative versions of the galactose gene network in the genus Saccharomyces and their expansion after domestication.

When Saccharomyces cerevisiae grows on mixtures of glucose and galactose, galactose utilization is repressed by glucose, and induction of the GAL gene network only occurs when glucose is exhausted. Contrary to reference GAL alleles, alternative alleles support faster growth on galactose, thus enabling distinct galactose utilization strategies maintained by balancing selection. Here, we report on new wild populations of Saccharomyces cerevisiae harboring alternative GAL versions and, for the first time, of Saccharomyces paradoxus alternative alleles. We also show that the non-functional GAL version found earlier in Saccharomyces kudriavzevii is phylogenetically related to the alternative versions, which constitutes a case of trans-specific maintenance of highly divergent alleles. Strains harboring the different GAL network variants show different levels of alleviation of glucose repression and growth proficiency on galactose. We propose that domestication involved specialization toward thriving in milk from a generalist ancestor partially adapted to galactose consumption in the plant niche.

Taxogenomic analyses of Starmerella gilliamiae f.a, sp. nov. and Starmerella monicapupoae f.a., sp. nov., two novel species isolated from plant substrates and insects.

Four yeast isolates collected from flowers from different ecosystems in Brazil, one from fruit of Nothofagus alpina in Argentina, three from flowers of Neltuma chilensis in Chile and one obtained from the proventriculus of a female bumblebee in Canada were demonstred, by analysis of the sequences of the internal transcribed spacer (ITS) region and D1/D2 domains of the large subunit rRNA gene, to represent two novel species of the genus Starmerella. These species are described here as Starmerella gilliamiae f.a, sp. nov. (CBS 16166T; Mycobank MB 851206) and Starmerella monicapupoae f.a., sp. nov. (PYCC 8997T; Mycobank MB 851207). The results of a phylogenomic analysis using 1037 single-copy orthogroups indicated that S. gilliamiae is a member of a subclade that contains Starmerella opuntiae, Starmerella aceti and Starmerella apicola. The results also indicated that S. monicapupoae is phylogenetically related to Starmerella riodocensis. The two isolates of S. monicapupoae were obtained from flowers in Brazil and were probably vectored by insects that visit these substrates. Starmerella gilliamiae has a wide geographical distribution having been isolated in flowers from Brazil and Chile, fruit from Argentina and a bumblebee from Canada.

Yeasts from tropical forests: Biodiversity, ecological interactions, and as sources of bioinnovation.

Tropical rainforests and related biomes are found in Asia, Australia, Africa, Central and South America, Mexico, and many Pacific Islands. These biomes encompass less than 20% of Earth's terrestrial area, may contain about 50% of the planet's biodiversity, and are endangered regions vulnerable to deforestation. Tropical rainforests have a great diversity of substrates that can be colonized by yeasts. These unicellular fungi contribute to the recycling of organic matter, may serve as a food source for other organisms, or have ecological interactions that benefit or harm plants, animals, and other fungi. In this review, we summarize the most important studies of yeast biodiversity carried out in these biomes, as well as new data, and discuss the ecology of yeast genera frequently isolated from tropical forests and the potential of these microorganisms as a source of bioinnovation. We show that tropical forest biomes represent a tremendous source of new yeast species. Although many studies, most using culture-dependent methods, have already been carried out in Central America, South America, and Asia, the tropical forest biomes of Africa and Australasia remain an underexplored source of novel yeasts. We hope that this review will encourage new researchers to study yeasts in unexplored tropical forest habitats.

The marula and elephant intoxication myth: assessing the biodiversity of fermenting yeasts associated with marula fruits (Sclerocarya birrea).

The inebriation of wild African elephants from eating the ripened and rotting fruit of the marula tree is a persistent myth in Southern Africa. However, the yeasts responsible for alcoholic fermentation to intoxicate the elephants remain poorly documented. In this study, we considered Botswana, a country with the world's largest population of wild elephants, and where the marula tree is indigenous, abundant and protected, to assess the occurrence and biodiversity of yeasts with a potential to ferment and subsequently inebriate the wild elephants. We collected marula fruits from over a stretch of 800 km in Botswana and isolated 106 yeast strains representing 24 yeast species. Over 93% of these isolates, typically known to ferment simple sugars and produce ethanol comprising of high ethanol producers belonging to Saccharomyces, Brettanomyces, and Pichia, and intermediate ethanol producers Wickerhamomyces, Zygotorulaspora, Candida, Hanseniaspora, and Kluyveromyces. Fermentation of marula juice revealed convincing fermentative and aromatic bouquet credentials to suggest the potential to influence foraging behaviour and inebriate elephants in nature. There is insufficient evidence to refute the aforementioned myth. This work serves as the first work towards understanding the biodiversity marula associated yeasts to debunk the myth or approve the facts.

Trabalho e riscos de adoecimento na Atenção Psicossocial Territorial: implicações para a gestão do cuidado em saúde mental / Work and illness risks in Territorial Psychosocial Care: implications for mental health care management

RESUMO O estudo busca analisar a precarização do trabalho por meio das condições laborais que influenciam a gestão do cuidado em saúde mental e a saúde do trabalhador. Trata-se de estudo de caso único, com abordagem mista, realizado em seis Centros de Atenção Psicossocial (Caps) de Área Descentralizada de Saúde. Aplicou-se o Inventário sobre Trabalho e Riscos de Adoecimento em amostra intencional total de 35 trabalhadores, dos quais, 15 participaram da entrevista projetiva. Os dados foram tratados no SPSS 26.0.0.0, expressos como medidas de tendência central e dispersão. As entrevistas foram categorizadas a partir dos eixos de avaliação propostos pelo inventário, contextualizadas e problematizadas a partir do pensamento complexo de Edgar Morin. O resultado é crítico para a maioria dos preditores que avaliaram o contexto, o custo humano, o prazer, o sofrimento e os danos relacionados ao trabalho nos Caps. Dados ilustrados pelas narrativas dos trabalhadores descrevem as condições do trabalho precário. Princípios produtivistas do neoliberalismo foram incorporados rapidamente pelos gestores locais do Sistema Único de Saúde, tornando a precarização do trabalho uma constante real. É preciso rever os processos de gestão do cuidado em saúde mental, financiamento e condições ocupacionais e contratuais legais, para que se alinhem com a Atenção Psicossocial Territorial (APT).

ABSTRACT The study aims to analyze precarious work due to working conditions that influence the management of mental health care and occupational health. This single case mixed study was conducted in six Psychosocial Care Centers (CAPS) in a Decentralized Health Sector. The Work and Illness Risks Inventory was applied to a total purposeful sample of 35 workers, 15 of whom participated in the projective interview. Data were processed in SPSS 26.0.0.0 as measures of central tendency and dispersion. The interviews were categorized based on the evaluation axes proposed by the inventory, contextualized, and discussed from Edgar Morin's Complex Thought. The result is critical for most predictors that evaluated the context, human cost, pleasure, distress, and harm related to work in the CAPS. Data illustrated by the workers' narratives describe the precarious work conditions. Local Unified Health System managers quickly incorporated neoliberalism's productivist principles, perpetuating substandard work conditions. Mental health care management processes, funding, and legal occupational and contractual conditions must be reviewed to align with Territorial Psychosocial Care (APT).

Do microbes evade domestication? - Evaluating potential ferality among diastatic Saccharomyces cerevisiae.

Certain lineages of the wine, beer and bread yeast Saccharomyces cerevisiae have diastatic activity. They contain the chimeric gene STA1 that codes for an extracellular glucoamylase which enables the strains to degrade starch and dextrins. Beer contaminations by diastatic yeasts can be dangerous because they can cause super-attenuation due to the consumption of otherwise non-fermentable oligosaccharides, gushing and off-flavours. Given that diastatic yeasts can be used for beer fermentation it is important to understand the relationship between production and contaminant strains, their natural reservoirs and entry routes into the brewery. Here, we analyze real cases of contamination in a Portuguese craft brewery over a period of 18 months. By analyzing with whole genome sequencing several contaminants, we show that recurrent contaminations by diastatic yeasts are caused by environmental strains. Moreover, some beer contaminants were closely related to diastatic environmental strains isolated in Botswana. We observed the widespread presence of domestication signatures in diastatic strains. Moreover, the combined phylogeny of STA1 and its ancestor, SGA1, suggested a single STA1 origin, as ancient as the entire lineage of diastatic yeasts. Together, our results suggest that diastatic yeasts isolated in natural settings could be escaping from domestication settings and becoming feral.

Genomic and ecological factors shaping specialism and generalism across an entire subphylum.

Organisms exhibit extensive variation in ecological niche breadth, from very narrow (specialists) to very broad (generalists). Paradigms proposed to explain this variation either invoke trade-offs between performance efficiency and breadth or underlying intrinsic or extrinsic factors. We assembled genomic (1,154 yeast strains from 1,049 species), metabolic (quantitative measures of growth of 843 species in 24 conditions), and ecological (environmental ontology of 1,088 species) data from nearly all known species of the ancient fungal subphylum Saccharomycotina to examine niche breadth evolution. We found large interspecific differences in carbon breadth stem from intrinsic differences in genes encoding specific metabolic pathways but no evidence of trade-offs and a limited role of extrinsic ecological factors. These comprehensive data argue that intrinsic factors driving microbial niche breadth variation.

A complexidade do trabalho precário na Atenção Psicossocial Territorial: reflexão crítica sobre o contexto brasileiro / The complexity of precarious work in Territorial Psychosocial Care: critical reflection on the Brazilian context

RESUMO Este artigo propõe compreender, na perspectiva da Teoria da Complexidade, e por meio da reflexão teórica crítica, a precarização do trabalho enquanto realidade no mercado brasileiro do trabalho público da saúde e sua influência nos resultados de uma política pública baseada no paradigma teórico/ prático da Atenção Psicossocial Territorial. Traça uma possibilidade de problematização sobre o tema central, por meio dos princípios: sistêmico, hologramático, círculo retroativo, recursivo, autonomia/ independência, dialógico e o da reintrodução do conhecimento em todo conhecimento, propostos por Edgar Morin, diante das discussões e dos estudos realizados pelo Grupo de Pesquisa Vida e Trabalho sobre a precarização do trabalho e a saúde mental em campo concreto. Essa reflexão propõe-se a ser fonte de reconhecimento para os atores, sobre as causas reais da precarização, que derivam do modo de exploração do trabalho em saúde em estreitamente próprio da aplicação do receituário neoliberal em estados burocráticos autoritários situados na periferia do capitalismo dependente. A reflexão permitiu a compreensão do todo e das partes que constituem o problema, reconhecendo as causas reais da precarização, ao contrário da culpabilização dos trabalhadores da saúde mental, diante das condições insalubres, da jornada de trabalho e da insegurança sobre os direitos sociais e direitos trabalhistas.

ABSTRACT This article proposes to understand, from the perspective of Complexity Theory, and through critical theoretical reflection, the precarization of work as a reality in the Brazilian public health work market, and its influence on the results of a public policy based on the theoretical/practical paradigm of Territorial Psychosocial Care. It outlines a possibility of problematizing the central theme, through the principles: systemic, hologrammatic, retroactive circle, recursive, autonomy/independence, dialogic, and the reintroduction of knowledge in all knowledge, proposed by Edgar Morin, in view of the discussions and studies carried out by the Life and Work Research Group on the precariousness of work and mental health in a concrete field. This reflection is intended to be a source of recognition for actors on the real causes of precariousness, which derive from the way in which health work is exploited, which is closely related to the application of neoliberal prescriptions in authoritarian bureaucratic states located on the periphery of dependent capitalism. The reflection allowed the understanding of the whole and the parts that constitute the problem, recognizing the real causes of precariousness, which blame mental health workers, in the face of unhealthy conditions, working hours, and insecurity about social rights and labor rights.

Macroevolutionary diversity of traits and genomes in the model yeast genus Saccharomyces.

Species is the fundamental unit to quantify biodiversity. In recent years, the model yeast Saccharomyces cerevisiae has seen an increased number of studies related to its geographical distribution, population structure, and phenotypic diversity. However, seven additional species from the same genus have been less thoroughly studied, which has limited our understanding of the macroevolutionary events leading to the diversification of this genus over the last 20 million years. Here, we show the geographies, hosts, substrates, and phylogenetic relationships for approximately 1,800 Saccharomyces strains, covering the complete genus with unprecedented breadth and depth. We generated and analyzed complete genome sequences of 163 strains and phenotyped 128 phylogenetically diverse strains. This dataset provides insights about genetic and phenotypic diversity within and between species and populations, quantifies reticulation and incomplete lineage sorting, and demonstrates how gene flow and selection have affected traits, such as galactose metabolism. These findings elevate the genus Saccharomyces as a model to understand biodiversity and evolution in microbial eukaryotes.

Hannaella floricola sp. nov., a novel basidiomycetous yeast species isolated from a flower of Lantana camara in Portugal.

During a survey of floricolous yeasts in Portugal, a basidiomycetous yeast representing a novel species in the genus Hannaella was isolated in Portugal from the flower of Lantana camara, an ornamental exotic species native to Central and South America. A combination of phylogenetic analyses of DNA barcode sequences used in yeast molecular systematics, namely the D1/D2 domain and the complete internal transcribed spacer (ITS) region supported the recognition of a new species of Hannaella, that we designate Hannaella floricola sp. nov. (ex-type strain PYCC 9191T=CBS 18097T). Although the assignment of the new species to the genus Hannaella was evident, the detection of its closest relatives appeared more problematic. Nevertheless, our analyses suggested that H. floricola sp. nov. belongs a clade that also includes H. coprosmae, H. oryzae and H. surugaensis, together four candidate novel species. In addition we provide the molecular identification of several unidentified strains whose D1/D2 and ITS sequences are available from GenBank.

A glimpse at an early stage of microbe domestication revealed in the variable genome of Torulaspora delbrueckii, an emergent industrial yeast.

Microbe domestication has a major applied relevance but is still poorly understood from an evolutionary perspective. The yeast Torulaspora delbrueckii is gaining importance for biotechnology but little is known about its population structure, variation in gene content or possible domestication routes. Here, we show that T. delbrueckii is composed of five major clades. Among the three European clades, a lineage associated with the wild arboreal niche is sister to the two other lineages that are linked to anthropic environments, one to wine fermentations and the other to diverse sources including dairy products and bread dough (Mix-Anthropic clade). Using 64 genomes we assembled the pangenome and the variable genome of T. delbrueckii. A comparison with Saccharomyces cerevisiae indicated that the weight of the variable genome in the pangenome of T. delbrueckii is considerably smaller. An association of gene content and ecology supported the hypothesis that the Mix-Anthropic clade has the most specialized genome and indicated that some of the exclusive genes were implicated in galactose and maltose utilization. More detailed analyses traced the acquisition of a cluster of GAL genes in strains associated with dairy products and the expansion and functional diversification of MAL genes in strains isolated from bread dough. In contrast to S. cerevisiae, domestication in T. delbrueckii is not primarily driven by alcoholic fermentation but rather by adaptation to dairy and bread-production niches. This study expands our views on the processes of microbe domestication and on the trajectories leading to adaptation to anthropic niches.

O cuidado em saúde mental e a participação política de usuários e familiares na ressignificação do estigma sobre os transtornos mentais / Mental health care and the political participation of users and families in the resignification of stigma on mental disorders

Resumo A persistência do estigma associado aos transtornos mentais deve ser um problema a ser enfrentado pelos sistemas de saúde na luta contra a discriminação de pessoas que experienciam sofrimento psíquico. Este estudo qualitativo, do tipo narrativo, objetiva compreender a experiência de conviver com o estigma relacionado aos transtornos mentais a partir dos relatos de usuários e de familiares de pessoas atendidas em Centros de Atenção Psicossocial de diferentes regiões da cidade de Fortaleza-CE. Foram entrevistados quatro usuários e três familiares que guardavam a particularidade de pertencerem a um núcleo da luta antimanicomial. Por meio de um roteiro com perguntas sobre as experiências individuais na busca por cuidados, as informações produzidas foram analisadas pelo método da hermenêutica dialética. Os resultados mostram que o estigma se faz presente no cotidiano, implicando sofrimento e discriminação. Contudo, os processos de cuidado recebidos, orientados para o reconhecimento dos potenciais e da condição de sujeitos políticos, ressignificaram percepções negativas sobre si mesmos e sobre os serviços em que eram atendidos. Esse processo colaborou com o engajamento dos usuários e dos familiares nas instâncias oficiais de controle social e no movimento antimanicomial, instigando, entre os sujeitos, a defesa do cuidado humanizado e do combate ao estigma.

Abstract The persistence of stigma associated with mental disorders is a problem that health systems must face in the fight against discrimination of people who experience psychological distress. This qualitative narrative study aims to understand the experience of living with the stigma related to mental disorders from the reports of users and family members of people assisted in Psychosocial Care Centers in different regions of the city of Fortaleza-CE. Four users and three family members who kept the particularity of belonging to a nucleus of the anti-asylum struggle were interviewed. Through a script with questions about individual experiences in the search for care, the information produced was analyzed by the method of dialectical hermeneutics. The results show that stigma is still present in everyday life, implying suffering and discrimination of these people. However, the mental health care processes received, which were oriented towards the recognition of the potential and condition of political subjects, gave new meaning to negative perceptions about themselves and about the services in which they were treated. This process contributed with the engagement of users and family members in official instances of social control and in the anti-asylum movement, instigating, among the subjects, the defense of humanized care and the fight against stigma.

Modelos e paradigmas orientadores do trabalho multiprofissional em Centros de Atenção Psicossocial em um município brasileiro / Models and guiding paradigms of multiprofessional work in Psychosocial Care Centers in a Brazilian municipality

Resumo Introdução Os Centros de Atenção Psicossocial Territorial, apesar de serem serviços substitutivos à internação psiquiátrica hospitalar, vem sendo afetado por práticas retrógradas, as quais dificultam o trabalho interdisciplinar e humanizado. Objetivo Compreender os modelos de atenção e os paradigmas existentes que orientam o processo de trabalho em saúde mental em Centros de Atenção Psicossocial Territorial. Métodos Estudo qualitativo, com abordagem reflexiva e crítica. Participaram do estudo 15 trabalhadores de nível médio e superior que atuavam nos Centros de Atenção Psicossocial Territorial de um município do estado do Ceará, Brasil, cujos relatos, coletados por meio de entrevistas individuais, semiestruturadas, as quais foram gravadas, tiveram seus corpos textuais tratados pelo software Iramuteq. Resultados As reflexões sobre as evidências empíricas nos conduzem para a institucionalização e enraizamento do modelo psiquiátrico clássico, sob a roupagem do modelo de atenção psicossocial territorial. Apesar de não haver mais barreiras e o aprisionamento físico dos usuários da Rede de Atenção Psicossocial territorial, estes vem se tornando dependentes do cuidado quase que exclusivo dentro dos serviços, sob forte caracter biomédico, pela dificuldade em desenvolver o processo de trabalho interdisciplinar de base territorial, havendo práticas pontuais de cuidado e prescrição medicamentosa. Considerações finais Torna-se preciso retomar o percurso reformista, o qual fortifique a estrutura da Política Nacional de Saúde Mental, considerando além do usuários, os trabalhadores, por meio da educação permanente em saúde, na garantia dos direitos sociais e trabalhistas, os quais favorecam a efetivação do modelo de Atenção Psicossocial Territorial.

Abstract Introduction The Territorial Psychosocial Care Centers, despite being substitutive services for psychiatric hospitalization, have been affected by retrograde practices, which make interdisciplinary and humanized work difficult. Objective To understand the care models and existing paradigms that guide the mental health work process in the Territorial Psychosocial Care Centers. Methods Qualitative study, with a reflective and critical approach. Participated in the study 15 secondary and higher education workers who worked in the Territorial Psychosocial Care Centers of a county in the state of Ceará, Brazil, whose reports, collected through individual, semi-structured interviews, which were recorded, had their textual bodies treated by the Iramuteq software. Results Reflections on empirical evidence lead us to the institutionalization and rooting of the classic psychiatric model, under the guise of the territorial psychosocial care model. Although there are no more barriers and the physical imprisonment of users of the Territorial Psychosocial Care Network, they have become dependent on almost exclusive care within the services, under a strong biomedical character, due to the difficulty in developing the interdisciplinary work process of territorial basis, with specific practices of care and drug prescription. Final considerations It is necessary to resume the reformist path, which strengthens the structure of the National Mental Health Policy, considering, in addition to the users, the workers, through permanent health education, in the guarantee of social and labor rights, which favor the effectiveness of the Territorial Psychosocial Care model.

Analysis of the geological control on the spatial distribution of potentially toxic concentrations of As and F- in groundwater on a Pan-European scale.

The distribution of the high concentrations of arsenic (As) and fluoride (F-) in groundwater on a Pan-European scale could be explained by the geological European context (lithology and structural faults). To test this hypothesis, seventeen countries and eighteen geological survey organizations (GSOs) have participated in the dataset. The methodology has used the HydroGeoToxicity (HGT) and the Baseline Concentration (BLC) index. The results prove that most of the waters considered in this study are in good conditions for drinking water consumption, in terms of As and/or F- content. A low proportion of the analysed samples present HGT≥ 1 levels (4% and 7% for As and F-, respectively). The spatial distribution of the highest As and/or F- concentrations (via BLC values) has been analysed using GIS tools. The highest values are identified associated with fissured hard rock outcrops (crystalline rocks) or Cenozoic sedimentary zones, where basement fractures seems to have an obvious control on the distribution of maximum concentrations of these elements in groundwaters.