RESUMO
El objetivo de este estudio fue la construcción y estudio piloto de un instrumento para evaluar factores asociados a la disposición hacia la donación de órganos. El Cuestionario sobre conocimientos, actitudes y creencias está conformado por 22 ítems tipo Likert de autoadministración. Fue respondido por 244 personas adultas apoderadas de 10 colegios de la provincia de Concepción, Chile, las cuales pertenecían a los grupos socioeconómicos bajo y medio. Se realizó un análisis factorial exploratorio (AFE) con el método de extracción de máxima verosimilitud y rotación varimax, el cual arrojó cinco factores: a) confianza en el proceso de donación y trasplante; b) comunicación familiar sobre donación de órganos; c) creencias ideológicas alrededor de la donación de órganos; d) conocimientos sobre donación y trasplante de órganos, y e) autonomía en la decisión de donar. La confiabilidad fue medida mediante el cálculo del coeficiente Alfa de Cronbach y la relación entre los factores mediante el coeficiente r de Pearson. Se establece que el instrumento es pertinente y confiable y se propone una nueva aplicación con una muestra más grande y que considere personas del grupo socioeconómico alto.
The object of this study was the construction and pilot study of an instrument for evaluating factors associated to individuals' willingness towards organ donation. The Questionnaire about Knowledge, Attitudes and Beliefs consists of 22 items Likert type of self administration. It was answered by 244 adults, guardians of 10 schools from Concepcion, Chile, who belonged to the middle and lower socioeconomic levels. An Exploratory Factor Analysis was done under the method of Maximum Likelihood Estimation and Varimax rotation, which derived in five factors: a) reliance on the process of donation and transplantation, b) communication about organ donation within the family, c) ideological beliefs on organ donation, d) knowledge about organ donation and transplantation, and e) autonomy on the choice of donation. The reliance was measured through the Cronbach's alpha and the relationship among the factors, through r of Pearson. It was established that the instrument is pertinent and reliable and a new application is proposed, to be administered on a bigger sample that includes individuals from the higher socioeconomic levels.
Assuntos
Obtenção de Tecidos e ÓrgãosRESUMO
Here, we evaluated the prevalence of Human Papillomavirus (HPV) in two groups of Chilean women. The first group consisted of 3235 women aged 18-64 years attended in six primary care centers of Santiago. The second group consisted of 456 women 18-85 aged who consulted the Gynaecology Department of the Reference Hospital of Santiago. Samples were collected from October 2012 to February 2016. Cervical swabs were analyzed both HPV genotyping by PCR and Reverse Line Blot, and cervical cytology by Pap testing. Results showed a prevalence of 12.0% HPV positive, 10.3% high-risk (HR) HPV types positive, 3.9% low-risk (LR) HPV types positive, and 1.0% Pap positive in group 1. The most frequent types were 16, 66, and 59, with a prevalence of 3.0%, 1.6%, and 1.5%, respectively. The prevalence were 71.9% HPV positive, 67.3% HR-HPV types positive, 13.6% LR-HPV types positive, and 62.5% Pap positive in group 2. The most frequent types were 16, 31, and 58, with prevalence of 33.6%, 10.5%, and 7.0%, respectively. Among infected women with HPV: 7.6% were infected with HPV16 or HPV18, 3.0% with HPV31, HPV33 or HPV45, and 6.7% with any other HR-HPV. These findings show great difference in HPV prevalence and types between primary care and reference center, and provide useful epidemiological information to assess the impact of HPV vaccination in the future.
Assuntos
Colo do Útero/virologia , Genótipo , Papillomaviridae/classificação , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/epidemiologia , Infecções por Papillomavirus/virologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Chile/epidemiologia , Feminino , Técnicas de Genotipagem , Humanos , Pessoa de Meia-Idade , Teste de Papanicolaou , Papillomaviridae/genética , Prevalência , Inquéritos e Questionários , Adulto JovemRESUMO
Infection with human T-lymphotropic virus type 1/2 (HTLV-1/2) is a major health problem. HTLV-1/2 infection is endemic in Chile but representative donor prevalence data are lacking. Data on all blood donors in a large network of Chilean blood centers were examined during 2011-2013. Screening of HTLV-1/2 antibodies were measured by enzyme immunoassay (EIA) at all blood banks. Blood samples with anticoagulants from initially reactive blood donors were analyzed by serological confirmation tests (immunofluorescence or recombinant immunoblot) at the HTLV National Reference Laboratory of the Public Health Institute of Chile. Additionally, detection of HTLV-1 and HTLV-2 provirus in peripheral blood mononuclear cells (PBMCs) was performed in all blood donors as confirmatory test. Prevalence rates were calculated. Among 694,016 donors, 706 were seropositive for HTLV-1 (prevalence, 1.02 cases per 1,000; 95% confidence interval [CI], 0.94-1.09), and 97 were seropositive for HTLV-2 (prevalence, 0.14 cases per 1,000; 95%CI, 0.11-0.17). Prevalence of HTLV-1 differed considerably by region, from 0.51 to 1.69 per 1,000. Prevalence of HTLV-2 was similar across the country (0.12-0.16). HTLV-1 prevalence was associated with female sex, older age, and residence in the north of Chile. HTVL-2 prevalence was associated with older age. The HTLV-1 prevalence among Chilean blood donors was relatively high and could be reduced by improving donor recruitment and selection in high prevalence areas. Blood center data may contribute to surveillance for HTLV-1 and HTLV-2 infections.
