1.
Bioorg Med Chem Lett
; 12(2): 177-80, 2002 Jan 21.
Artigo
em Inglês
| MEDLINE
| ID: mdl-11755348
RESUMO
A new and more efficient route to the synthesis of branched PEG for protein conjugation, bearing a reporter dipeptide Met-betaAla, is described, which allows better purification of the final product by ion exchange chromatography. The product has the combined advantages of an 'umbrella-like' branched structure, which allows a better coverage of the protein surface, and the presence of the dipeptide Met-betaAla which has been used to detect the position of PEGylation within the peptide sequence.