Histomorfometria do epitélio ruminal de cabritos 1/2 sangue Boer submetidos a dietas com tortas oriundas da produção do biodiesel / Histomorphometrics of the ruminal epithelium of 1/2 blood Bôer goats submitted to diets with pies from the production of biodiesel

Objetivou-se determinar o melhor nível de inclusão e de substituição das tortas de dendê e amendoim, respectivamente, em dietas para cabritos 1/2 sangue Boer, por meio da avaliação histomorfométrica das papilas ruminais. Foram utilizados 40 cabritos 1/2 sangue Boer, para cada período experimental, machos, não castrados, com idade aproximada de três meses e com peso inicial de 15,01±1,76kg. Os períodos experimentais constaram de 75 dias e 72 dias. As dietas consistiram de volumoso feno de Tifton-85 e de ração em mistura completa, contendo níveis de inclusão da torta de dendê nas proporções de 0,0; 7,0; 14,0; e 21,0% com base na matéria seca; e de mistura completa, contendo níveis de substituição do farelo de soja pela torta de amendoim nas proporções de 0,0; 33,0; 66,0; e 100%, constituindo-se os tratamentos. No epitélio do rúmen, foram avaliadas altura e largura das papilas, assim como densidade papilar e espessura da parede muscular do rúmen. Entre estas, a altura das papilas, no experimento com torta de amendoim, foi a única a sofrer efeito linear decrescente (P<0,0014); as demais variáveis, tanto dos animais alimentados com torta de amendoim quanto daqueles alimentados com torta de dendê, não foram afetadas pela dieta. As médias de altura encontradas foram de 2,6 e 2,3mm para as tortas de amendoim e dendê, respectivamente. Portanto, a inclusão de até 21% de torta de dendê e a substituição de 100% de torta de amendoim no concentrado de cabritos não alteraram a morfometria das papilas ruminais.(AU)

The aim of this study was to determine the best level of pies and replacement of palm oil and peanut, respectively, in diets of 1/2 blood Bôer goats from the histomorphometry of the rumen papillae. A total of 40 1/2 blood male, unneutered, aged approximately three months and initial weight of 15.01±1.76kg Bôer goats were used in each experimental period. The experimental periods consisted of 75 days and 72 days. The diets consisted of roughage hay Tifton-85 and total mixed ration containing levels of inclusion of palm kernel cake in the proportions of 0.0, 7.0, 14.0 and 21.0% based on dry matter; and the second experiment consisted of a complete mixture containing substitution levels of soybean meal by groundnut cake in the proportions of 0.0, 33.0, 66.0 and 100%. Epithelial cells were evaluated in the rumen height and width of the papillae, and density and thickness of the papillary muscle of the rumen. Among these, the height of the papillae in the experiment with peanut butter pie was the only one to suffer a negative linear effect (P<0.0014), the other variables, both in animals fed groundnut cake and palm oil, were not affected by the diet. The average heights found were 2.6 and 2.3, for pies, peanut and palm oil, respectively. Therefore, the inclusion of up to 21% palm kernel cake and replacement of 100% peanut cake in the concentrate of kids did not alter the morphology of the rumen papillae.(AU)

Histomorfometria do epitélio ruminal de cabritos 1/2 sangue Boer submetidos a dietas com tortas oriundas da produção do biodiesel / Histomorphometrics of the ruminal epithelium of 1/2 blood Bôer goats submitted to diets with pies from the production of biodiesel

Objetivou-se determinar o melhor nível de inclusão e de substituição das tortas de dendê e amendoim, respectivamente, em dietas para cabritos 1/2 sangue Boer, por meio da avaliação histomorfométrica das papilas ruminais. Foram utilizados 40 cabritos 1/2 sangue Boer, para cada período experimental, machos, não castrados, com idade aproximada de três meses e com peso inicial de 15,01±1,76kg. Os períodos experimentais constaram de 75 dias e 72 dias. As dietas consistiram de volumoso feno de Tifton-85 e de ração em mistura completa, contendo níveis de inclusão da torta de dendê nas proporções de 0,0; 7,0; 14,0; e 21,0% com base na matéria seca; e de mistura completa, contendo níveis de substituição do farelo de soja pela torta de amendoim nas proporções de 0,0; 33,0; 66,0; e 100%, constituindo-se os tratamentos. No epitélio do rúmen, foram avaliadas altura e largura das papilas, assim como densidade papilar e espessura da parede muscular do rúmen. Entre estas, a altura das papilas, no experimento com torta de amendoim, foi a única a sofrer efeito linear decrescente (P<0,0014); as demais variáveis, tanto dos animais alimentados com torta de amendoim quanto daqueles alimentados com torta de dendê, não foram afetadas pela dieta. As médias de altura encontradas foram de 2,6 e 2,3mm para as tortas de amendoim e dendê, respectivamente. Portanto, a inclusão de até 21% de torta de dendê e a substituição de 100% de torta de amendoim no concentrado de cabritos não alteraram a morfometria das papilas ruminais.

The aim of this study was to determine the best level of pies and replacement of palm oil and peanut, respectively, in diets of 1/2 blood Bôer goats from the histomorphometry of the rumen papillae. A total of 40 1/2 blood male, unneutered, aged approximately three months and initial weight of 15.01±1.76kg Bôer goats were used in each experimental period. The experimental periods consisted of 75 days and 72 days. The diets consisted of roughage hay Tifton-85 and total mixed ration containing levels of inclusion of palm kernel cake in the proportions of 0.0, 7.0, 14.0 and 21.0% based on dry matter; and the second experiment consisted of a complete mixture containing substitution levels of soybean meal by groundnut cake in the proportions of 0.0, 33.0, 66.0 and 100%. Epithelial cells were evaluated in the rumen height and width of the papillae, and density and thickness of the papillary muscle of the rumen. Among these, the height of the papillae in the experiment with peanut butter pie was the only one to suffer a negative linear effect (P<0.0014), the other variables, both in animals fed groundnut cake and palm oil, were not affected by the diet. The average heights found were 2.6 and 2.3, for pies, peanut and palm oil, respectively. Therefore, the inclusion of up to 21% palm kernel cake and replacement of 100% peanut cake in the concentrate of kids did not alter the morphology of the rumen papillae.

Professionalism, patient satisfaction and quality of health care: experience during Zimbabwe's structural adjustment programme.

In 1991, Zimbabwe embarked on a structural adjustment programme. In the health sector, collection of fees was enforced and fees were later increased. Utilisation subsequently declined. This paper examines the perceptions of both government nurses and health care consumers regarding the impact of adjustment on overall quality of care, including nurse professionalism, the nurse-client relationship and patient satisfaction with care. These issues were explored in a series of focus group discussions held in December 1993, about three years after policy reforms. The discussions suggested many areas of shared concern (fees, drug availability, waiting times), but divergent views regarding the process of care. Nurses were concerned mainly with overwork and patient ingratitude, and failed to recognise nurse behaviour as a major source of patient dissatisfaction. Community women saw nurses as hardened and indifferent, especially in urban areas. These differences are rooted in the perceived class differences between nurses and the communities they serve, but appear to have sharpened during the period of structural adjustment.

Low birth weight in urban Harare.

OBJECTIVES: 1. To measure the incidence of low birth weight from all institutional deliveries in a defined catchment area of urban Harare. 2. To estimate the relative proportions of preterm and small for gestational age (SGA). DESIGN: The study was descriptive and was conducted during the last three months of 1986. SETTING: The low risk maternity units in three high density suburbs, Highfield, Glen Norah and Glen View and the referral centre Harare Central Hospital. SUBJECTS: All babies born to women residing in the study areas in the maternity clinics and those transferred to the referral hospital were identified and weighed. MAIN OUTCOME MEASURES: For low birthweight babies gestation was estimated by the Dubowitz method and perinatal outcome was recorded. RESULTS: During the three month study period in 1986, 2,056 babies in total were born; 223 (10.8%) of which were low birthweight. Of these 65 (44%) were preterm and 55 (37%) were SGA. CONCLUSION: The findings show a lower percentage of low birthweight and SGA babies than in many developing countries but higher than the norm for developed countries. The study demonstrates a need for resources to prevent low birthweight delivery and improve care for low birthweight babies.

PIP: A 1986 descriptive study of all institutional deliveries in a defined catchment area of urban Harare, Zimbabwe, investigated the incidence of low birth weight (LBW, under 2500 grams), and the relative contributions of preterm and small-for-gestational-age (SGA, gestational age 37 weeks or above plus LBW) deliveries to LBW. Since most LBW statistics are based on deliveries from central hospitals that serve as referral centers for distant rural areas, they cannot be considered representative of a particular urban population. The high-density, low-income Harare suburbs of Highfield, Glen Norah, and Glen View were selected for the analysis. During the 3-month study period, there were 2056 registered births in the 3 communities. 1101 of these deliveries occurred at the 3 low-risk maternal clinics serving these areas and 955 took place, after referral, at the high-risk Harare Central Hospital. There were 223 LBW infants (10.8%), 50 of whom were delivered in the clinics and 173 in the referral hospital. All 17 infants weighing under 1000 grams were delivered at the hospital. 148 LBW infants (including 13 stillbirths, 6 early neonatal deaths, and 14 sets of twins) were selected for further study. 44% of these infants were preterm and 37% were SGA; the remainder were appropriate for gestational age. When the data were adjusted to conform with the Villa/Belizan categories, 5.3% were LBW/preterm and 5.5% were LBW/intrauterine growth retardation. Overall, the LBW pattern identified in this study falls between that of a developed and a developing country, which is presumably reflective of the high level of general development in Harare.

Community-based survey versus sentinel site sampling in determining the nutritional status of rural children. Implications for nutritional surveillance and the development of nutritional programmes.

A study of the anthropometric status of under-5-year-olds was conducted in the Nqutu district of KwaZulu by means of a representative community-based sample and concurrent samples from primary health care clinics, preschools and primary schools. The first objective of this exercise was to determine the extent of acute nutritional stress in the district as an indication of the appropriateness of food relief efforts in the region. The absence of wasting and the high prevalence of stunting (37.5%) in the community-based sample suggested that the main problem is chronic socio-economic underdevelopment, rather than a severe or immediate lack of food. The fact that fewer than 20% of households are in any way reliant on domestic production for their maize requirements explains why the recent drought has not had a greater impact on the nutritional status of this vulnerable group. This study confirms that the more recent emphasis of the National Nutrition and Social Development Programme on social development is appropriate. An equally important objective of this study was to evaluate the usefulness of clinics, preschools and schools as sites for the collection of anthropometric data and the development of nutritional programmes. This preliminary attempt to develop the methodology for district-based nutrition surveillance suggested that all these sites have limitations both in respect of data collection and community access. The implications of using these sites and the developments needed to improve their usefulness in a future nutrition surveillance system are discussed.

First report from the Plunket National Child Health Study: smoking during pregnancy in New Zealand.

The Plunket National Child Health Study is a 5-year longitudinal study of a birth cohort of 4285 children born in New Zealand during 1990-1991. This paper describes the major lines of epidemiological research, the methods and study design, and reports on the demographic data of New Zealand children. During this first report from the Plunket Child Health Study, we examine on the smoking rates of New Zealand mothers during pregnancy. Overall 33% of mothers smoked during pregnancy. Particularly high rates were found amongst teenage mothers,. Maori women, single women and women with lower educational levels. Over 60% of women in these categories smoked during pregnancy.

Estimating child mortality in Zimbabwe: results of a pilot study using the preceding births technique.

Using the preceding birth technique, 2,229 mothers were interviewed at four antenatal centres (two of which were urban) over a three month period during a pilot study in Zimbabwe. Results of the study showed that there was a small difference between the under two and under five mortality. The smaller than expected difference could have been due to some degree of urbanisation being experienced at two of the centres, and to a systematic selection bias of the method. Other findings of the study showed that younger mothers (under 20 years of age) and older mothers (over 40 years) experienced higher proportions of mortality, than mothers in the age group between. The higher proportion of male mortality compared to that for females reflected the expected trend, but the size of the difference was somewhat surprising, and could have been due to recall bias. The mean birth interval was 36 months, rather than the expected 30 months, and analysis of mortality in relation to birth interval and maternal age showed that a birth interval of less than 18 months was associated with higher child mortality, significantly so with the second last child, independent of mother's age. Longer birth intervals (more than three years) among older mothers were associated with higher changes of child survival. It is concluded that the Brass-Macrae method is a useful technique to analyse levels and trends of child mortality.

PIP: The University of Zimbabwe and the Zimbabwe Congress of Trade Unions tested the ability of the Brass-Macrae methodology (i.e., preceding birth technique) to estimate current levels and trends in child mortality. Interviews were conducted with 2229 mothers attending the prenatal clinics of the Mbare and Glen View polyclinics in Harare and of a provincial hospital (Marondera) and district hospital (Mutoko), both in Mashonaland East Province. The overall child mortality rate was 53/1000 of the last live births and 54/1000 of the second last live births. Some degree of urbanization and a systematic selection bias of the method may have contributed to the small difference between the two rates. Among last live births, the provincial hospital in Marondera had the lowest child mortality rate, while the Mbare polyclinic in the capital city of Harare had the highest (43 vs. 61). The difference in child mortality rates for last live births between urban and rural areas was slight, while it was much greater for second last live births (2 vs. 11 points). Among second last live births, the district hospital in Mutoko had the lowest rate, while the Marondera provincial hospital had the highest rate (39 vs. 64). Child mortality levels peaked in the mothers' age groups less than 20 (88 for last live births) and more than 40 (84 for last live births and 78 for second last live births). Among last and second last live births, boys were more likely to die than girls (64 vs. 42, p 0.05, and 64 vs. 45, p 0.05, respectively). This difference could have been due to boys being more frail than girls or mothers being able to better recall boys deaths, since boys are highly valued. The mean birth interval was 36 months, but the expected interval was 30 months. A birth interval of less than 18 months was significantly associated with higher child mortality among second last live births (p 0.001), regardless of mother's age. Birth intervals of more than 3 years were associated with the best child survival rates in every maternal age group (e.g., 30-34 years, 8 vs. 52-250). This pilot study showed that the Brass-Macrae method is useful to estimate child mortality.

Tuberculosis presenting as corticosteroid responsive wheezing in infancy.

A nine-month-old child received intermittent oral prednisolone over a 6 month period because of persistent wheezing. This therapy had appeared to improve his symptoms. At 15 months of age, a chest X-ray and culture of tracheal aspirate revealed disseminated pulmonary tuberculosis. Response to antituberculous therapy was slow, and withdrawal of steroids produced marked worsening of wheeze and respiratory distress. Corticosteroids are recognised adjuncts in the management of bronchial-lymph node tuberculosis. Responsiveness of wheezing to prednisolone does not imply that its cause is hyperreactive airways disease, and other causes should be sought particularly in developing countries.

Light regulation of a SSRubisco-nos chimaeric gene: photoregulatory control sequences from a C3 plant function in cells of a CAM plant.

A SSRubisco-nos chimaeric gene has been constructed, in an oncogenic Ti-plasmid vector. A 900bp soybean SSRubisco upstream fragment, carrying CAAT and TATA boxes and transcription initiation point, was fused to the nos coding region, the fusion site being within the 5'-untranslated region. When this chimaeric construct was transferred to Kalanchoe cells, nos expression was shown to be light-regulated. Thus DNA sequences responsible for light-dark control of gene expression are wholly or partly contained within the 900bp soybean SSRubisco upstream region. Moreover, this is the first demonstration that photoregulatory elements in a gene derived from a C3 plant, function in cells of a plant exhibiting the CAM trait.

Body image stereotypes: a developmental comparison.

The purpose of this study was to test the hypothesis that temperament judgments associated with different body types were dependent upon sex and age of the respondents. A total of 500 participants, 249 females and 251 males ranging from 6 to 60 years of age, were sampled. Participants were drawn from various civic, educational, institutional, and residential groups in a Southern cosmopolitan city. A paper-pencil instrument consisted of three body silhouettes (representing an ectomorph, mesomorph, and endomorph) and 40 temperament/behavior descriptors. The respondents matched each descriptor with one of the three body types. The results indicated that the descriptors were differentially associated with body types according to age groups but not by sex. The mesomorph was overwhelmingly and consistently viewed as the most positive of the three body types, but was rated somewhat more negatively as the age of the group increased. Participants of ages 6 through 25 rated the endomorph more negatively than the ectomorph, while those of ages 26-40 rates them equally. In contrast, participants over 40 years of age viewed the ectomorph more negatively than the endomorph.