[Effect of C60 fullerene on metabolic and proliferative activity of PKE cell line].

The effect of C60 fullerene aqueous colloid solution (C60 FAS) on activity of redox and proliferative processes in PKE (transplantable cell line of pig kidney embryo) cells has been studied. In particular, it was established that the presence of C60 fullerene (127 microM) in culturing medium of PKE cells during 48 h did not change their ability to reduce non-toxic AlamarBlue redox indicator and proliferative activity.

[Investigation of adhesive properties of polypropylene net prosthesis "ESPERA"].

Adhesive towards the cells properties of three polypropylene net prostheses (PNP), manufactured by "Espera", with the slots size 1 x 1 mm, 2 x 2 mm, 2 x 4 mm and one specimen (the slot diameter 1 mm) on the oxycellulose background, were tested for prognostication of hernioplasty results, using nonresorbable PNP. The adhesion degree and peculiarities of cellular line, obtained from embryonal kidney of pig, put on the tested nets specimen in conditions of cultivation during 5 - 10 days were estimated, using inverted microscope in regime of phasic contrast. The data obtained witness the lowered cellular adhesion towards PNP "Espera" without oxycellular background. The net processing, using components of extracellular matrix, as well as presence of the oxycellulose fragments, facilitate the PNP ingrowth by connective tissue.

[Cryosurgery, reloading and renewal].

The work constitutes an attempt to analyze and to determine the causes of problems concerning the cryosurgical methods of treatment development as well as the possible ways of their solving, Along with vivid progress in cryosurgical technologies taking place, cryosurgery is developing actively and need the conduction of profound scientific investigations in this field. It is possible to reveal a real potential of cryosurgery after scientific experimental--practical base creation, aiming at introduction of cryosurgical methods in a full volume into clinical medicine.

The use of catalytic carbon deposits as 3D carriers for human bone marrow stromal cells.

We studied the possibility of using 3D structures based on carbon catalytic deposits as carriers for human bone marrow stromal cells. It was found that carbon catalytic deposits obtained by gas deposition method using FeCl(3) × 6H(2)O as the catalyst are a biocompatible material for human bone marrow stromal cells promoting adhesion, proliferation, and distribution of cells within the 3D carrier, and therefore can be used for tissue engineering.

[Cryosurgery in diffuse hepatic diseases].

Comparative studying, using histological and biomicroscopic methods, of the dosed cryohepatodestruction (CHD), periarterial cryodenervation of hepatic artery (CDHA) and their concomitant application influence on the dynamics of hepatic restoration processes in experimental cirrhosis was performed. The investigations were done on 215 male rats owing body mass 200-280 g in a not changed and pathologically changed liver. There was shown, that CDHA promotes changes in hepatic tissue microhemocirculation, as well as the enhancement of the sinusoidal vessels diameter and relative square of vascular bed. CHD stimulates the reparative processes course in a pathologically changed organ. There was established, that while simultaneous application of two cryosurgical methods, the velocity and grade of restoration processes in cirrhotically-changed liver are enhanced in comparison with such indices changes while separate usage of these two methods.

[Surgical treatment of complications of insulin-dependent (type-1) diabetes mellitus]. / Khirurgicheskoe lechenie oslozhnenii insulinzavisimogo (I tip) sakharnogo diabeta.

In the clinic, within the recent 5 years, the surgical treatment of complication of diabetes mellitus were used in 88 patients. Xenotransplantation of islet pancreatic tissue was performed in 70 patients, deportalization of pancreatic blood flow--in 18. Resulting from the operations performed, a pronounced antidiabetic effect (reduction of daily insulin dosage, stabilization of the incidence of complications) was noted in the majority of patients. Occasionally, the staged combined use of both the methods for surgical treatment of complications of diabetes mellitus is possible.

[Treatment of frostbite]. / Lechenie otmorozhenii.

The article shows that treatment of frostbites in a special medium with controlled temperature and humidity makes the time of treatment shorter and prevents complications characteristic of such a trauma. The main purpose of such exposures of the injured areas is to achieve mummification and to make the operation as early as possible. The mummification of the soft tissues began within 5-8 days after trauma. Chemical necrectomy was possible on the 7th-9th days, followed by autodermoplasty on the 2nd day.

[Effectiveness of cryogenic action in experimental liver resection]. / Effektivnost' kriovozdeistviia pri rezektsii pecheni v éksperimente.

The traditional ligature methods were added with freezing of the left hepatic lobe resection line in order to block up parenchymatous bleeding and cholerhagia in atypical resection of the liver. Alterations in the enzymatic activity (alaninic and asparaginic transaminases alkaline phosphotase, lactate dehydrogenase and ribonuclease) in the blood serum and liver of the rabbits allowed to judge about the character of inflammatory and destructive changes in the liver following the use of the abovementioned methods. The rise of the activity of the enzymes under study within the first days after operation and its normalization by the 7th to 14th days in all the studied variants of hemo- an cholestasis have been established.