RESUMO
This study investigated the influence of glutathione S-transferase omega 1 (GSTO1) and GSTO2 gene polymorphisms on susceptibility and aggressiveness of head and neck squamous cell carcinoma (HNSCC). A case-control study consisting of 300 HNSCC cases and 299 age and sex- matched normal control was performed. Genotyping of GSTO1*A140D and GSTO2*N142D polymorphisms was determined using the polymerase chain reaction-restriction fragment length polymorphism method. Our results revealed that the frequencies of GSTO1 and GSTO2 genotypes were not significantly different between HNSCC cases and controls. No significant differences were found in smoking or drinking status between cases and controls. However, HNSCC individuals with the GSTO1*D140 varient were significantly associated with nodal metastasis (OR = 0.53, 95 %CI = 0.31-0.91, P = 0.020) and advanced pathological stage (OR = 0.33,95 %CI = 0.15-0.70, P = 0.032), while no significant association was observed between GSTO2 genotype and clinicopathological features. Therefore, our findings suggest that the GSTO1*D140 variant genotype in individuals might play a protective role against the aggressiveness of HNSCC.
Assuntos
Carcinoma de Células Escamosas/enzimologia , Carcinoma de Células Escamosas/genética , Progressão da Doença , Estudos de Associação Genética , Predisposição Genética para Doença , Glutationa Transferase/genética , Neoplasias de Cabeça e Pescoço/enzimologia , Neoplasias de Cabeça e Pescoço/genética , Polimorfismo de Nucleotídeo Único/genética , Carcinoma de Células Escamosas/patologia , Estudos de Casos e Controles , Feminino , Frequência do Gene/genética , Neoplasias de Cabeça e Pescoço/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Carcinoma de Células Escamosas de Cabeça e PescoçoRESUMO
PURPOSE: To evaluate the association between glutathione S-transferase Omega (GSTO) genes polymorphism and the susceptibility of acute lymphoblast leukemia (ALL). METHODS: The polymorphism of GSTO1 and GSTO2 genes were analyzed in 99 ALL patients compared with 100 healthy children by PCR-based restriction fragment length polymorphism (RFLP) analysis. RESULTS: GSTO1*A140D polymorphism was significantly associated with susceptibility to ALL (OR = 2.24, 95% CI = 1.16-4.35, P = 0.009) whereas, GSTO2*N142D genotype was significantly interacted with high risk group of childhood ALL (OR = 5.52, 95% CI = 1.72-17.71, P = 0.004). CONCLUSION: This study revealed gene polymorphism in glutathione S-transferase Omega class may be a risk factor to the development of acute childhood lymphoblastic leukemia.
Assuntos
Glutationa Transferase/genética , Polimorfismo Genético , Polimorfismo de Nucleotídeo Único , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Adolescente , Criança , Pré-Escolar , Códon/genética , DNA/genética , DNA/isolamento & purificação , DNA de Neoplasias/genética , DNA de Neoplasias/isolamento & purificação , Éxons/genética , Feminino , Humanos , Lactente , Masculino , Leucemia-Linfoma Linfoblástico de Células Precursoras/enzimologia , Valores de Referência , Estudos RetrospectivosRESUMO
Oral cancer, one of the ten most widespread cancers in Thailand, is a major public health problem. The aim of the study was to assess hMSH2 and hMLH1 gene mutations, microsatellite DNA alterations, and investigate the association between these alterations and clinicopathological features of oral squamous cell carcinomas (SCC) in a sample of Thai patients. Microsatellite alterations at D2S391, D3S647, D17S513, and D17S520 were detected at a frequency of 40.6%. Among these alterations, 12.5% exhibited loss of heterozygosity (LOH) at D3S647 and D17S513, while 34.4% exhibited microsatellite instability (MI) at D2S391, D17S513, and D17S520. Polymorphic change in the intronic region of hMSH2 at IVS 1 nt 211+9, c-->g was observed in 50% of cases. Significant correlation was observed between IVS 1 nt 211+9 polymorphism and the recurrence status of the patients (p = 0.030, OR = 10.67). This study demonstrated that the polymorphism of hMSH2 at IVS 1 nt 211+9 (c-->g) was associated with oral cancer recurrence status and could be used as a biomarker for prognosis and follow-up treatment of oral cancer.
