[The many faces of contact in caring]. / As várias faces do contato no cuidar.

This paper emerged from a group experience about a theme related to human care: the contact. The experience is described, unveiling feelings and memories perceived while constructing the activity, building a concept of contact, here seen as one of the fundaments of human care. Human care was studied on an anthropological approach, lived in a group technique. Our meanings of contact were expressed with the words: senses, sensibility, representation, confidence, relationship. Each significance was worked and detailed since our concept of contact arose from the interrelation of them. Some theoretical reflection about the theme was done and constructs of this kind of care were elaborated, since it is considered one of the fundaments of human care.

Release of lactate dehydrogenase activity by rabbit renal cortex slices during storage in solutions used for kidney transplantation.

Lactate dehydrogenase (LDH) activity was evaluated after incubation of rabbit renal cortical slices in several preservation solutions. Experimental solutions commonly used for kidney transplantation decreased LDH activity up to 72 h of storage at 4 degrees C compared to a control plasma-like solution. Low temperature (4 degrees C) storage led to a smaller release of LDH than slices preserved at 25 degrees C in control solutions. The addition of organic substrates to the control solution diminished enzyme release only of those slices incubated at 25 degrees C. After 72 h of incubation, the rate of LDH release was constant for all solutions except Collins glucose 330 mOsm solution for which the rate increased still further up to 96 h. The lower rate of LDH release exhibited by experimental solutions for up to 72 h indicates the maintenance of cellular integrity and hence of tissue viability. On the other hand, the continued increase of the rate of LDH release shown by Collins glucose 300 mOsm solution up to 96 h may suggest an even higher survival of viable cells and the stabilization of the rate of LDH release observed in the latter period of storage may indicate the onset of necrosis.