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1.
J Heart Valve Dis ; 22(1): 126-32, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23611000

RESUMO

BACKGROUND AND AIM OF THE STUDY: Prosthetic valve endocarditis (PVE) after aortic valve replacement occurs infrequently but carries a high mortality rate, particularly with previous valved conduit root replacement or aortic root reconstruction (ARR). Infection can lead to paravalvular aortic root abscess, aorto-left ventricular disruption, and left ventricular pseudoaneurysm formation. Herein is presented a case series of aortic-left ventricular disruption and ventricular psuedoaneurysm secondary to PVE after previous aortic root replacement; the surgical approach and outcomes are discussed. METHODS: All patients who underwent cardiac valve surgery at The Methodist DeBakey Heart and Vascular Center between October 2008 and May 2011 were reviewed for cases of PVE with previous ARR with valved conduit and aortic root replacement. Five cases were identified, for whom the record review and follow up by clinic visit or telephone call after discharge was complete. RESULTS: All patients survived surgical repair after complete redo aortic root excision and reconstruction with valved-conduit and coronary reimplantation. All patients underwent delayed sternal closure with mediastinal packing and, in four cases, definitive closure with omental flap. All patients were discharged and remain recurrence-free with the current management scheme. CONCLUSION: Aortic left ventricular disruption from PVE after aortic root replacement with valved conduit can be managed successfully. The authors' strategy includes careful sternal entry with preparations for emergency bypass, complete excision of all infected material, redo total aortic root replacement and coronary reimplantation, initial open sternal management to control coagulopathy, and definitive closure with an omental transposition flap in a delayed fashion.


Assuntos
Doenças da Aorta/cirurgia , Endocardite/cirurgia , Doenças das Valvas Cardíacas/cirurgia , Implante de Prótese de Valva Cardíaca , Complicações Pós-Operatórias/cirurgia , Adulto , Idoso , Doenças da Aorta/etiologia , Endocardite/complicações , Feminino , Doenças das Valvas Cardíacas/complicações , Humanos , Masculino , Pessoa de Meia-Idade , Recidiva , Reoperação
2.
J Immunol ; 189(6): 2843-51, 2012 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-22888136

RESUMO

Despite advances in renal replacement therapy, the mortality rate for acute kidney injury (AKI) remains unacceptably high, likely owing to extrarenal organ dysfunction. Kidney ischemia-reperfusion injury (IRI) activates cellular and soluble mediators that facilitate organ crosstalk and induce caspase-dependent lung apoptosis and injury through a TNFR1-dependent pathway. Given that T lymphocytes mediate local IRI in the kidney and are known to drive TNFR1-mediated apoptosis, we hypothesized that T lymphocytes activated during kidney IRI would traffic to the lung and mediate pulmonary apoptosis during AKI. In an established murine model of kidney IRI, we identified trafficking of CD3+ T lymphocytes to the lung during kidney IRI by flow cytometry and immunohistochemistry. T lymphocytes were primarily of the CD3+CD8+ phenotype; however, both CD3+CD4+ and CD3+CD8+ T lymphocytes expressed CD69 and CD25 activation markers during ischemic AKI. The activated lung T lymphocytes did not demonstrate an increased expression of intracellular TNF-α or surface TNFR1. Kidney IRI induced pulmonary apoptosis measured by caspase-3 activation in wild-type controls, but not in T cell-deficient (T(nu/nu)) mice. Adoptive transfer of murine wild-type T lymphocytes into T(nu/nu) mice restored the injury phenotype with increased cellular apoptosis and lung microvascular barrier dysfunction, suggesting that ischemic AKI-induced pulmonary apoptosis is T cell dependent. Kidney-lung crosstalk during AKI represents a complex biological process, and although T lymphocytes appear to serve a prominent role in the interorgan effects of AKI, further experiments are necessary to elucidate the specific role of activated T cells in modulating pulmonary apoptosis.


Assuntos
Injúria Renal Aguda/imunologia , Movimento Celular/imunologia , Isquemia/imunologia , Pulmão/imunologia , Pulmão/patologia , Ativação Linfocitária/imunologia , Subpopulações de Linfócitos T/imunologia , Subpopulações de Linfócitos T/patologia , Injúria Renal Aguda/patologia , Animais , Modelos Animais de Doenças , Isquemia/patologia , Pulmão/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos Nus , Camundongos Transgênicos , Traumatismo por Reperfusão/imunologia , Traumatismo por Reperfusão/patologia
3.
Am J Physiol Lung Cell Mol Physiol ; 303(5): L449-59, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22728466

RESUMO

Despite advancements in renal replacement therapy, the mortality rate for acute kidney injury (AKI) remains unacceptably high, likely due to remote organ injury. Kidney ischemia-reperfusion injury (IRI) activates cellular and soluble mediators that incite a distinct pulmonary proinflammatory and proapoptotic response. Tumor necrosis factor receptor 1 (TNFR1) has been identified as a prominent death receptor activated in the lungs during ischemic AKI. We hypothesized that circulating TNF-α released from the postischemic kidney induces TNFR1-mediated pulmonary apoptosis, and we aimed to elucidate molecular pathways to programmed cell death. Using an established murine model of kidney IRI, we characterized the time course for increased circulatory and pulmonary TNF-α levels and measured concurrent upregulation of pulmonary TNFR1 expression. We then identified TNFR1-dependent pulmonary apoptosis after ischemic AKI using TNFR1-/- mice. Subsequent TNF-α signaling disruption with Etanercept implicated circulatory TNF-α as a key soluble mediator of pulmonary apoptosis and lung microvascular barrier dysfunction during ischemic AKI. We further elucidated pathways of TNFR1-mediated apoptosis with NF-κB (Complex I) and caspase-8 (Complex II) expression and discovered that TNFR1 proapoptotic signaling induces NF-κB activation. Additionally, inhibition of NF-κB (Complex I) resulted in a proapoptotic phenotype, lung barrier leak, and altered cellular flice inhibitory protein signaling independent of caspase-8 (Complex II) activation. Ischemic AKI activates soluble TNF-α and induces TNFR1-dependent pulmonary apoptosis through augmentation of the prosurvival and proapoptotic TNFR1 signaling pathway. Kidney-lung crosstalk after ischemic AKI represents a complex pathological process, yet focusing on specific biological pathways may yield potential future therapeutic targets.


Assuntos
Injúria Renal Aguda/metabolismo , Apoptose , Isquemia/metabolismo , Pulmão/patologia , Receptores Tipo I de Fatores de Necrose Tumoral/metabolismo , Traumatismo por Reperfusão/metabolismo , Injúria Renal Aguda/sangue , Injúria Renal Aguda/complicações , Animais , Proteína Reguladora de Apoptosis Semelhante a CASP8 e FADD/metabolismo , Permeabilidade Capilar , Caspase 3/metabolismo , Caspase 8/metabolismo , Creatinina/sangue , Etanercepte , Imunoglobulina G/farmacologia , Isquemia/sangue , Isquemia/complicações , Rim/irrigação sanguínea , Rim/fisiopatologia , Lesão Pulmonar/etiologia , Lesão Pulmonar/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , NF-kappa B/antagonistas & inibidores , NF-kappa B/metabolismo , Nitrilas/farmacologia , Isoformas de Proteínas/metabolismo , Receptores do Fator de Necrose Tumoral , Traumatismo por Reperfusão/sangue , Traumatismo por Reperfusão/complicações , Transdução de Sinais , Sulfonas/farmacologia , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Fator de Necrose Tumoral alfa/sangue
4.
J Am Coll Surg ; 214(4): 629-37; discussion 637-9, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22321526

RESUMO

BACKGROUND: Preoperative imaging in patients with primary hyperparathyroidism provides important localization information. Although 4-dimensional neck CT (4DCT) can precisely localize hyperfunctioning parathyroid tissue, the contribution of 4DCT to overall cost, operating room time, and hospital stay is unknown. STUDY DESIGN: Records of 535 patients with primary hyperparathyroidism who underwent parathyroidectomy at our institution from 1996 to 2010 were reviewed. All patients had preoperative cervical ultrasonography and sestamibi scanning, and most (78.9%) underwent preoperative 4DCT. A decision tree was constructed to compare extent of procedure, operating room time, length of stay, failure rate, and total cost of each strategy (with and without 4DCT). Costs were determined by 2010 Medicare reimbursement. RESULTS: For patients with and without preoperative 4DCT, respectively, mean operating room time (64.4 vs 61.4 minutes; p = 0.58) and failure rate (1.9% vs 4.4%; p = 0.12) were not significantly different. Length of stay was higher in the no-CT cohort (0.61 vs 0.23 days; p < 0.001). Patients with a preoperative 4DCT were significantly more likely to undergo a limited parathyroidectomy (90.3% vs 80.5%; p = 0.004). Mean cost of care per patient in the CT and no-CT cohorts was $6,572 and $6,306, respectively. CONCLUSIONS: The introduction of routine 4DCT into the preoperative workup for surgical intervention in primary hyperparathyroidism does not appear to shorten operating room time or decrease failure rate significantly. However, preoperative 4DCT is associated with shorter hospital stays and improved rates of minimally invasive parathyroidectomy. This clinical benefit must be weighed against the increased cost associated with routine preoperative 4DCT.


Assuntos
Tomografia Computadorizada Quadridimensional , Custos Hospitalares , Hiperparatireoidismo Primário/diagnóstico por imagem , Paratireoidectomia , Cuidados Pré-Operatórios , Análise Custo-Benefício , Árvores de Decisões , Tomografia Computadorizada Quadridimensional/economia , Humanos , Hiperparatireoidismo Primário/economia , Hiperparatireoidismo Primário/cirurgia , Tempo de Internação/economia , Tempo de Internação/estatística & dados numéricos , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Minimamente Invasivos/economia , Modelos Estatísticos , Paratireoidectomia/economia , Paratireoidectomia/métodos , Cuidados Pré-Operatórios/economia , Cuidados Pré-Operatórios/métodos , Texas , Fatores de Tempo , Falha de Tratamento , Resultado do Tratamento
5.
Eplasty ; 11: e8, 2011 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-21369366

RESUMO

OBJECTIVE: To determine alterations in quantities and distributions of natural antimicrobials following ischemia-reperfusion injury. We hypothesized that these compounds would be upregulated in areas of small intestine where changes in permeability and cellular disruption were likely and where protective mechanisms would be initiated. METHODS: Rats with ischemia-reperfusion underwent superior mesenteric artery clamping and reperfusion. Shams were subjected to laparotomy but no clamping. Ileum and jejunum were harvested and sectioned, and subjected to fluorescence deconvolution microscopy for determinations of content and localization of rat beta defensins, 1, 2, 3; rat neutrophil protein-1; and cathelicidin LL-37. Modeling was performed to determine cellular location of antimicrobials. RESULTS: Ischemia-reperfusion increased neutrophil defensin alpha (RNP-1) in jejunum; rat beta defensin 1 was increased 2-fold in ileal mucosa and slightly reduced in jejunal mucosa; rat beta defensin 2 was reduced by ischemia-reperfusion in ileum, but slightly increased in jejunum; rat beta defensin 3 was concentrated in the muscularis externa and myenteric plexus of the jejunum; ischemia-reperfusion did not alter cathelicidin LL-37 content in the small intestine, although a greater concentration was seen in jejunum compared with ileum. CONCLUSION: Ischemia-reperfusion injury caused changes in antimicrobial content in defined areas, and these different regulations might reflect the specific roles of jejunum versus ileum.

7.
Mol Cell Biochem ; 347(1-2): 191-9, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21046201

RESUMO

We previously demonstrated that enteral arginine increased c-Jun/activator protein-1 (AP-1) DNA-binding activity and iNOS expression in a rodent model of mesenteric ischemia/reperfusion (I/R). The objective of this study was to specifically investigate the role of AP-1 in arginine's deleterious effect on the postischemic gut. We hypothesized that AP-1 inhibition would mitigate the effects of arginine. Using a rodent model of mesenteric I/R we demonstrated that gut neutrophil infiltration, activity of c-Jun/AP-1, as well as iNOS expression were increased by I/R and further increased by arginine while lessened by inhibition of c-Jun using the pharmacologic c-Jun N-terminal kinase inhibitor, SP600125. Similar results were demonstrated using a cell culture model of oxidant stress in IEC-6 cells. Importantly, effects of SP600125 were comparable to those of c-Jun silencing. Lastly, the specific iNOS inhibitor, 1400W, had no effect on either AP-1 or c-Jun. In conclusion, SP600125 attenuated the activity of c-Jun/AP-1, iNOS expression, and neutrophil infiltration induced by arginine following mesenteric I/R. Our data suggest that AP-1 inhibition mitigates the injurious inflammatory effects of arginine in the postischemic gut. Further investigation into the pathologic role of enteral arginine in the postischemic gut is warranted.


Assuntos
Antracenos/farmacologia , Arginina/administração & dosagem , Arginina/farmacologia , Intestino Delgado/irrigação sanguínea , Proteínas Proto-Oncogênicas c-jun/antagonistas & inibidores , Traumatismo por Reperfusão/patologia , Fator de Transcrição AP-1/antagonistas & inibidores , Amidinas/farmacologia , Animais , Benzilaminas/farmacologia , DNA/metabolismo , Nutrição Enteral , Inativação Gênica/efeitos dos fármacos , Inflamação , Intestino Delgado/efeitos dos fármacos , Intestino Delgado/enzimologia , Intestino Delgado/patologia , Óxido Nítrico Sintase Tipo II/metabolismo , Ligação Proteica/efeitos dos fármacos , Proteínas Proto-Oncogênicas c-jun/metabolismo , RNA Interferente Pequeno/metabolismo , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/enzimologia , Fator de Transcrição AP-1/metabolismo
8.
J Vasc Surg ; 52(4): 1003-14, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20678877

RESUMO

INTRODUCTION: Mesenteric ischemia-reperfusion injury (IRI) leads to systemic inflammation and multiple organ failure in clinical and laboratory settings. We investigated the lung structural, functional, and genomic response to mesenteric IRI with and without regional intraischemic hypothermia (RIH) in rodents and hypothesized that RIH would protect the lung and preferentially modulate the distant organ transcriptome under these conditions. METHODS: Sprague-Dawley rats underwent sham laparotomy or superior mesenteric artery occlusion (SMAO) for 60 minutes with or without RIH. Gut temperature was maintained at 15°-20°C during SMAO, and systemic normothermia (37°C) was maintained throughout the study period. At 6 or 24 hours, lung tissue was collected for (1) histology, (2) myeloperoxidase activity, (3) bronchoalveolar lavage (BAL) fluid protein concentrations, (4) lung wet/dry ratios, and (5) total RNA isolation and hybridization to Illumina's Sentrix BeadChips (>22,000 probes) for gene expression profiling. Significantly affected genes (false discovery rate <5% and fold change ≥1.5) were linked to gene ontology (GO) terms using MAPPFinder, and hypothermia-suppressed genes were further analyzed with Pubmatrix. RESULTS: Mesenteric IRI-induced lung injury, as evidenced by leukocyte trafficking, alveolar hemorrhage, and increased BAL protein and wet/dry ratios, and activated a proinflammatory lung transcriptome compared with sham. In contrast, rats treated with RIH exhibited lung histology, BAL protein, and wet/dry ratios similar to sham. At 6 hours, GO analysis identified 232 hypothermia-suppressed genes related to inflammation, innate immune response, and cell adhesion, and 33 hypothermia-activated genes related to lipid and amine metabolism and defense response. Quantitative real-time polymerase chain reaction validated select array changes in top hypothermia-suppressed genes lipocalin-2 (lcn-2) and chemokine ligand 1 (CXCL-1), prominent genes associated with neutrophil activation and trafficking. CONCLUSIONS: Therapeutic hypothermia during SMAO provides distant organ protection and preferentially modulates the IRI-activated transcriptome in the rat lung. This study identifies potential novel diagnostic and therapeutic targets of mesenteric IRI and provides a platform for further mechanistic study of hypothermic protection at the cellular and subcellular level.


Assuntos
Hipotermia Induzida , Pulmão/imunologia , Oclusão Vascular Mesentérica/terapia , Pneumonia/prevenção & controle , Traumatismo por Reperfusão/prevenção & controle , Animais , Líquido da Lavagem Broncoalveolar/imunologia , Adesão Celular/genética , Quimiotaxia de Leucócito/genética , Análise por Conglomerados , Modelos Animais de Doenças , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica , Imunidade Inata/genética , Mediadores da Inflamação/metabolismo , Pulmão/irrigação sanguínea , Pulmão/patologia , Masculino , Oclusão Vascular Mesentérica/complicações , Ativação de Neutrófilo/genética , Análise de Sequência com Séries de Oligonucleotídeos , Pneumonia/etiologia , Pneumonia/genética , Pneumonia/imunologia , Pneumonia/patologia , Reação em Cadeia da Polimerase , Edema Pulmonar/imunologia , Edema Pulmonar/prevenção & controle , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/etiologia , Traumatismo por Reperfusão/genética , Traumatismo por Reperfusão/imunologia , Traumatismo por Reperfusão/patologia , Reprodutibilidade dos Testes , Fatores de Tempo
9.
Ann Thorac Surg ; 89(3): 931-6; discussion 936-7, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20172156

RESUMO

BACKGROUND: Esophageal or gastric leakage from anastomotic wound dehiscence, perforation, staple line dehiscence, or trauma can be a devastating event. Traditional therapy has often consisted of either surgical repair for rapidly diagnosed leaks or diversion for more complicated cases, commonly associated with a delayed diagnosis. This study summarizes our experience treating leaks or fistulas with novel, covered self-expanding metal stents (cSEMS). The primary objective of this study was to determine the efficacy and safety of covered self-expanding metal stents when used to treat complicated leaks and fistulas. METHODS: Over 15 months, 25 patients with esophageal or gastric leaks were evaluated for stenting as primary treatment. A prospective database was used to collect data. Stents were placed endoscopically, with contrast evaluation used for leak evaluation. Patients who did not improve clinically after stenting or whose leak could not be sealed underwent operative management. RESULTS: During a mean follow-up of 15 months, 23 of the 25 patients with esophageal or gastric leaks during a 15-month period were managed with endoscopic stenting as primary treatment. Healing occurred in patients who were stented for anastomotic leakage after gastric bypass or sleeve gastrectomy (n = 10). One patient with three esophageal iatrogenic perforations healed with stenting. Eight patients successfully avoided esophageal diversion and healed with stenting and adjunctive therapy. Two of the 4 patients with tracheoesophageal fistulas sealed with the assistance of a new pexy technique to prevent stent migration; 1 additional patient had this same technique used to successfully heal an upper esophageal perforation. CONCLUSIONS: Esophageal leaks and fistulas can be effectively managed with cSEMS as a primary modality. The potential benefits of esophageal stenting are healing without diversion or reconstruction and early return to an oral diet.


Assuntos
Remoção de Dispositivo , Perfuração Esofágica/terapia , Esôfago , Stents , Fístula Traqueoesofágica/terapia , Anastomose Cirúrgica/efeitos adversos , Materiais Revestidos Biocompatíveis , Feminino , Humanos , Masculino , Metais , Pessoa de Meia-Idade , Stents/efeitos adversos
10.
J Surg Res ; 161(2): 288-94, 2010 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-20080249

RESUMO

Nutritional supplementation has become the standard of care for management of critically ill patients. Traditionally, nutritional support in this patient population was intended to replete substrate deficiencies secondary to stress-induced catabolism. Recognition of the influence of certain nutrients on the immune and inflammatory response of the critically ill has led to the evolution of more sophisticated nutritional strategies and concepts. Administration of immune-enhancing formulas supplemented with a combination of glutamine, arginine, omega-3 fatty acids (omega-3 FA), and nucleotides have been shown in most studies to reduce infectious outcomes. More recently, the separation of nutritional support from the provision of key nutrients has led to a further appreciation of the immunomodulatory and anti-inflammatory benefits of isolated nutrients, such as glutamine and antioxidants. The purpose of this article is to review the molecular mechanisms that are unique to each class of frequently utilized nutrients. A better understanding of the specific molecular targets of immunonutrients will facilitate application of more refined nutritional therapies in critically ill patients.


Assuntos
Nutrição Enteral/métodos , Apoio Nutricional , Aminoácidos/metabolismo , Animais , Antioxidantes/metabolismo , Arginina/metabolismo , Estado Terminal , Ácidos Graxos Ômega-3/metabolismo , Glutamina/metabolismo , Humanos , Nucleotídeos/metabolismo , PPAR gama/metabolismo , Receptores X de Retinoides/metabolismo
11.
Crit Care ; 13 Suppl 5: S10, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19951382

RESUMO

INTRODUCTION: The purpose of the present review is to review our experience with near-infrared spectroscopy (NIRS) monitoring in shock resuscitation and predicting clinical outcomes. METHODS: The management of critically ill patients with goal-oriented intensive care unit (ICU) resuscitation continues to evolve as our understanding of the appropriate physiologic targets improves. It is now recognized that resuscitation to achieve supranormal indices is not beneficial in all patients and may precipitate abdominal compartment syndrome. RESULTS: Over the years, ICU technology has provided physicians with specific physiologic parameters to guide shock resuscitation. Throughout this time, the tissue hemoglobin oxygen saturation (StO2) monitor has emerged as a non-invasive means to obtain reliable physiologic parameters to guide clinicians' resuscitative efforts. StO2 monitors have been shown to aid in early identification of nonresponders and to predict outcomes in hemorrhagic shock and ICU resuscitation. These data have also been used to better understand and refine existing resuscitation protocols. More recently, use of NIRS technology to guide resuscitation in septic shock has been shown to predict outcomes in high-risk patients. CONCLUSIONS: StO2 is an important tool in identifying high-risk patients in septic and hemorrhagic shock. It is a non-invasive means of obtaining vital information regarding outcome and adequacy of resuscitation.


Assuntos
Hemoglobinas/metabolismo , Unidades de Terapia Intensiva , Monitorização Fisiológica/métodos , Consumo de Oxigênio/fisiologia , Ressuscitação/métodos , Centros de Traumatologia , Animais , Humanos , Unidades de Terapia Intensiva/tendências , Monitorização Fisiológica/tendências , Oximetria/métodos , Ressuscitação/tendências , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Espectroscopia de Luz Próxima ao Infravermelho/tendências , Centros de Traumatologia/tendências
12.
Curr Opin Crit Care ; 15(6): 481-7, 2009 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19851101

RESUMO

PURPOSE OF REVIEW: Acute kidney injury (AKI) continues to contribute significantly to morbidity and mortality in the ICU setting, especially when associated with distant organ dysfunction. There is increasing evidence that AKI directly contributes to organ dysfunction in lung, brain, liver, heart and other organs. This review will examine our current understanding of the deleterious organ crosstalk in the critically ill, which can provide a framework for developing novel therapeutics. RECENT FINDINGS: The majority of studies correlating AKI with distant organ dysfunction have demonstrated the pathophysiological importance of proinflammatory and proapoptotic pathways as well as oxidative stress and reactive oxygen species (ROS) production. Leukocyte activation and infiltration, changes in levels of soluble factors such as cytokines and chemokines, and regulation of cell death in extra-renal organs are potentially important mechanisms by which AKI modulates multiorgan dysfunction. SUMMARY: There is increasing knowledge of AKI and deleterious interorgan crosstalk that arises, at least in part, due to the imbalance of immune, inflammatory, and soluble mediator metabolism that attends severe insults to the kidney. Further studies can build on these new mechanistic observations to develop strategies to improve outcomes in the critically ill patient.


Assuntos
Injúria Renal Aguda/fisiopatologia , Insuficiência de Múltiplos Órgãos/fisiopatologia , Injúria Renal Aguda/complicações , Injúria Renal Aguda/metabolismo , Humanos , Inflamação/fisiopatologia , Isquemia/fisiopatologia , Insuficiência de Múltiplos Órgãos/metabolismo
13.
Curr Opin Crit Care ; 14(6): 679-84, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19005309

RESUMO

PURPOSE OF REVIEW: To review what we learned through implementation of computerized decision support for ICU resuscitation of major torso trauma patients who arrive in shock. RECENT FINDINGS: Overall, these patients respond well to preload-directed goal-orientated ICU resuscitation; however, the subset of patients destined to develop abdominal compartment syndrome do not respond well. In fact, this strategy precipitates the full-blown syndrome that is a new iatrogenic variant of multiple organ failure. The clinical trajectory of abdominal compartment syndrome starts early after emergency department admission and its course is fairly well defined by the time patients reach the ICU. It occurs in patients who arrive with severe bleeding that is not readily controlled. These patients require a very different emergency department management strategy. Hemorrhage control is paramount. Alternative massive transfusion protocols should be used with an emphasis on hemostasis and avoidance of excessive isotonic crystalloids. Finally, near-infrared spectroscopy that measures tissue hemoglobin saturation in skeletal muscle (StO2) is good at identifying high-risk patients. A falling StO2 in the setting of ongoing resuscitation is a harbinger of death from early exsanguination and multiple organ failure. SUMMARY: Fundamental changes are needed in the care of trauma patients who arrive in shock and require a massive transfusion.


Assuntos
Síndromes Compartimentais/terapia , Cuidados Críticos/métodos , Sistemas de Apoio a Decisões Clínicas/organização & administração , Choque Hemorrágico/terapia , Ferimentos e Lesões/complicações , Transfusão de Sangue , Síndromes Compartimentais/etiologia , Síndromes Compartimentais/fisiopatologia , Humanos , Unidades de Terapia Intensiva , Músculo Esquelético/fisiopatologia , Oxigenoterapia , Choque Hemorrágico/etiologia , Choque Hemorrágico/fisiopatologia , Espectroscopia de Luz Próxima ao Infravermelho , Texas
14.
Am J Physiol Gastrointest Liver Physiol ; 285(5): G1056-67, 2003 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-12855402

RESUMO

Smad proteins are transcription activators that are critical for transmitting transforming growth factor-beta (TGF-beta) superfamily signals from the cell surface receptors to the nucleus. Our previous studies have shown that cellular polyamines are essential for normal intestinal mucosal growth and that a decreased level of polyamines inhibits intestinal epithelial cell proliferation, at least partially, by increasing expression of TGF-beta/TGF-beta receptors. The current study went further to determine the possibility that Smads are the downstream intracellular effectors of activated TGF-beta/TGF-beta receptor signaling following polyamine depletion. Studies were conducted in IEC-6 cells derived from rat small intestinal crypts. Depletion of cellular polyamines by alpha-difluoromethylornithine (DFMO) increased basal levels of Smad3 and Smad4 proteins, induced their nuclear translocation, and stimulated Smad sequence-specific DNA-binding activity. Polyamine depletion-induced Smads were also associated with a significant increase in transcription activation as measured by luciferase reporter gene activity of Smad-dependent promoters. Inhibition of Smads by a dominant-negative mutant Smad4 in the DFMO-treated cells prevented the increased Smad transcription activation. Polyamine-deficient cells highly expressed TGF-beta and were growth-arrested at the G1 phase. Inhibition of TGF-beta by treatment with either immunoneutralizing anti-TGF-beta antibody or TGF-beta antisense oligodeoxyribonucleotides not only blocked the induction of Smad activity but also decreased the Smad-mediated transcriptional activation in polyamine-depleted cells. These findings suggest that Smads are involved in the downstream cellular processes mediated by cellular polyamines and that increased TGF-beta/TGF-beta receptor signaling following polyamine depletion activates Smads, thus resulting in the stimulation of Smad target gene expression.


Assuntos
Proteínas de Ligação a DNA/fisiologia , Mucosa Intestinal/metabolismo , Poliaminas/metabolismo , Transdução de Sinais/fisiologia , Transativadores/fisiologia , Fator de Crescimento Transformador beta/fisiologia , Animais , Divisão Celular/fisiologia , Linhagem Celular , DNA/metabolismo , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Eflornitina/farmacologia , Mucosa Intestinal/citologia , Ratos , Proteína Smad3 , Proteína Smad4 , Distribuição Tecidual , Transativadores/genética , Transativadores/metabolismo , Transcrição Gênica/fisiologia , Fator de Crescimento Transformador beta/antagonistas & inibidores , Fator de Crescimento Transformador beta/metabolismo
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