Age-specific and species-specific tree response to seasonal drought in tropical dry forests.

Millions of people depend on ecosystem services provided by Tropical Dry Forests (TDFs), yet their proximity to population centers, seasonally dry climate, and the ease at which they are converted to agriculture has left only 10 % of their original extent globally. As more TDFs become protected, basic information relating TDF age to subsurface water resources will help guide forest recovery. Severe deforestation and recent reforestation around Bahía de Caráquez, Ecuador produced a mosaic of different successional stages ideal for exploring relationships between TDF age, subsurface water availability and species-specific responses to seasonal drought. Over one year, we measured gravimetric water content, predawn and midday leaf water potential, and the stable isotope composition of xylem and source waters in two regenerating and one primary forest. Over the transition from wet to dry season, we discovered a sharper decrease in predawn water potential in younger successional forests than in the primary forest. Growing in degraded subsurface environments under increased competition, successional forest trees accessed deeper sources of moisture from unsaturated weathered bedrock and groundwater through the dry season; however, different species employed distinct water use strategies. Ceiba trichistandra maintained midday water potentials above -1.27 MPa through a drought avoidance strategy dependent on groundwater. Sideroxylon celastrinum tolerated drought by lowering predawn and midday water potential through the early dry season but took up greater proportions of saprolite moisture and groundwater as the dry season progressed. Contrastingly, Handroanthus chrysanthus maintained access to shallow soil and saprolite moisture by dropping midday water potential to -4.30 MPa, reflecting drought tolerance. Our results show that limited subsurface water resources in regenerating TDF's lead to species-specific adaptations reliant on deeper sources of moisture. The recovery of soil and saprolite hydrologic properties following disturbances is likely to exceed 100 years, highlighting the importance of forest conservation.

Unexpected nitrogen sources in a tropical urban estuary.

Tropical urban estuaries are severely understudied. Little is known about the basic biogeochemical cycles and dominant ecosystem processes in these waterbodies, which are often low-lying and heavily modified. The San Juan Bay Estuary (SJBE) in San Juan, Puerto Rico is an example of such a system. Over the past 80 years, a portion of the estuary has filled in, changing the hydrodynamics and negatively affecting water quality. Here we sought to document these changes using ecological and biogeochemical measurements of surface sediments and bivalves. Measurements of sediment physical characteristics, organic matter content, and stable isotope ratios (δ13C, δ15N, δ34S) illustrated the effects of the closure of the Caño Martín Peña (CMP) on the hydrology and water quality of the enclosed and semienclosed parts of the estuary. The nitrogen stable isotope (δ15N) values were lowest in the CMP, the stretch of the SJBE that is characterized by waters with low dissolved oxygen and high fecal coliform concentrations. Despite this, the results of this study indicate that nitrogen (N) contributions from N-fixing, sulfate-reducing microbes may meet or even exceed contributions from urban runoff and sewage. While the importance of sulfate reducers in contributing N to mangrove ecosystems is well documented, this is the first indication that such processes could be dominant in an intensely urban system. It also underscores just how little we know about tropical coastal ecosystems in densely populated areas throughout the globe.

Nitrogen and carbon concentrations and stable isotope ratios: Data from a 15N tracer study in short-form Spartina alterniflora and Distichlis spicata.

We present four datasets that provide information on primary production, nitrogen (N) uptake and allocation in two salt marsh grasses, short-form Spartina alterniflora and Distichlis spicata. These four datasets were generated during a month-long stable isotope (15N) tracer study described in the companion manuscript (Hill et al., 2018). They include an allometry dataset containing mass and height data for individual plants harvested from Colt State Park, Bristol, Rhode Island and used to nondestructively estimate plant masses. A second dataset contains weekly stem height measurements collected over the course of the 15N tracer study. Also included are high resolution data from 49 vegetated compartments (leaves, stems, fine/coarse roots, rhizomes) and bulk sediment depth intervals, reporting the mass, carbon and N concentrations, and stable isotope ratios measured following the harvest of cores over time. Additionally, we provide a complementary dataset with estimates of microbial removal from potential and ambient denitrification enzyme assays. These data, along with source code used in their analysis, are compiled in the NitrogenUptake2016 R package available from the Comprehensive R Archive Network.

Nitrogen uptake and allocation estimates for Spartina alterniflora and Distichlis spicata.

Salt marshes have the potential to intercept nitrogen that could otherwise impact coastal water quality. Salt marsh plants play a central role in nutrient interception by retaining N in above- and belowground tissues. We examine N uptake and allocation in two dominant salt marsh plants, short-form Spartina alterniflora and Distichlis spicata. Nitrogen uptake was measured using 15N tracer experiments conducted over a four-week period, supplemented with stem-level growth rates, primary production, and microbial denitrification assays. By varying experiment duration, we identify the importance of a rarely-measured aspect of experimental design in 15N tracer studies. Experiment duration had a greater impact on quantitative N uptake estimates than primary production or stem-level relative growth rates. Rapid initial scavenging of added 15N caused apparent nitrogen uptake rates to decline by a factor of two as experiment duration increased from one week to one month, although each experiment shared the qualitative conclusion that Distichlis roots scavenged N approximately twice as rapidly as Spartina. We estimate total N uptake into above- and belowground tissues as 154 and 277 mg N·m-2·d-1 for Spartina and Distichlis, respectively. Driving this pattern were higher N content in Distichlis leaves and belowground tissue and strong differences in primary production; Spartina and Distichlis produced 8.8 and 14.7 g biomass·m-2·d-1. Denitrification potentials were similar in sediment associated with both species, but the strong species-specific difference in N uptake suggests that Distichlis-dominated marshes are likely to intercept more N from coastal waters than are short-form Spartina marshes. The data and source code for this manuscript are available as an R package from https://github.com/troyhill/NitrogenUptake2016.