Multisystemic Inflammatory Syndrome Temporally Associated with COVID-19 in a Regional Pediatric Hospital from México.

Multisystemic inflammatory syndrome (MIS-C) is an inflammatory condition temporally associated with COVID-19 in children; nevertheless, the clinical and immunologic spectrum of MIS-C is heterogeneous, and its long-term effects are unknown. During the period of August 2020 to December 2021, a total of 52 MIS-C cases were confirmed in pediatric patients from the Hospital del Niño DIF Hidalgo, diagnosed using criteria from the World Health Organization. All patients had serologic IgG confirmation of SARS-CoV2, the mean age of the patients was 7 years, and 94% of the patients did not have a previous underlying disease. In addition to the presentation of lymphopenia, neutropenia, and thrombocytopenia, elevations in D-dimer and ferritin levels were observed in all patients. There was clinical improvement with intravenous gamma globulin and corticosteroid treatment.

Tenebrio molitor as a Clean Label Ingredient to Produce Nutritionally Enriched Food Emulsions.

Tenebrio molitor flour, a sustainable source of protein and bioactive compounds, was used as a clean label ingredient in order to reformulate a commercial hummus sauce, replacing egg yolk and modified starch, improving its nutritional quality. For this purpose, the impact of different concentrations of insect flour on the sauce was studied. Rheology properties, texture profile analysis, and the microstructure of the sauces were analyzed. Nutritional profile analysis was carried out, as well as bioactivity, namely the total phenolic content and the antioxidant capacity. Sensory analysis was conducted to determine the consumer's acceptance. At low concentrations (up to 7.5% of T. molitor flour) the sauce structure remained practically unchanged. However, for higher additions of T. molitor (10% and 15%), a loss of firmness, adhesiveness and viscosity was observed. Structure parameters such as elastic modulus (G') at 1 Hz of the sauces with 10% and 15% were significantly lower than the commercial sauce, indicating a loss of structure caused by Tenebrio flour incorporation. Although the formulation with 7.5% T. molitor flour was not the best rated in the sensory analysis, it showed a higher antioxidant capacity compared to the commercial standard. In addition, this formulation also presented the highest concentration in total phenolic compounds (16.25 mg GAE/g) and significantly increased the content of proteins (from 4.25% to 7.97%) and some minerals, compared to the standard.

Mendelian Susceptibility to Mycobacterial Disease: Retrospective Clinical and Genetic Study in Mexico.

Mendelian susceptibility to mycobacterial disease (MSMD) is a rare genetic disorder characterized by impaired immunity against intracellular pathogens, such as mycobacteria, attenuated Mycobacterium bovis-Bacillus Calmette-Guérin (BCG) vaccine strains, and environmental mycobacteria in otherwise healthy individuals. Retrospective study reviewed the clinical, immunological, and genetic characteristics of patients with MSMD in Mexico. Overall, 22 patients diagnosed with MSMD from 2006 to 2021 were enrolled: 14 males (64%) and eight females. After BCG vaccination, 12 patients (70%) developed BCG infection. Furthermore, 6 (22%) patients developed bacterial infections mainly caused by Salmonella, as what is described next in the text is fungal infections, particularly Histoplasma. Seven patients died of disseminated BCG disease. Thirteen different pathogenic variants were identified in IL12RB1 (n = 13), IFNGR1 (n = 3), and IFNGR2 (n = 1) genes. Interleukin-12Rß1 deficiency is the leading cause of MSMD in our cohort. Morbidity and mortality were primarily due to BCG infection.

Interesse da população pela jardinagem durante o isolamento social na pandemia da COVID-19 / Population's interest in gardening during social isolation in the COVID-19 Pandemic

Métodos associados à jardinagem tornaram-se uma boa alternativa de lazer para lidar com o isolamento social e as dificuldades geradas pela pandemia da COVID19. Com este estudo objetivou-se avaliar motivação da população para a prática da jardinagem antes e durante o isolamento social e suas principais contribuições. A coleta de dados foi realizada através de um questionário contendo 23 questões, e divulgado via internet. Os dados obtidos foram tabulados em planilha de Excel e posteriormente alguns dados foram analisados com o auxílio do software estatístico R versão 4.1.0 para Windows. Diante do presente estudo foi possível observar que a população acredita que o cultivo de plantas em casa melhora a qualidade de vida durante o confinamento da COVID-19, sendo uma opção de lazer onde muitos se beneficiaram, seja de forma física ou emocional, pelo contato com a natureza.

Methods associated with gardening have become a good leisure alternative to cope with social isolation and the difficulties generated by the pandemic of COVID-19. With this study we aimed to evaluate the population motivation to practice gardening before and during social isolation and its main contributions. The collecting of data was done through a questionnaire containing 23 questions, and disseminated via Internet. The data collected was tabulated in Excel spreadsheet and later some data were analyzed with the assistance of the statistical software R version 4.1.0 for Windows. In this study it was possible to observe that the population believes that growing plants at home improves the quality of life during the COVID-19 confinement, being a leisure option where many benefited, physically or emotionally, from the contact with nature.

Thermal comfort conditions at microclimate scale and surface urban heat island in a tropical city: A study on João Pessoa city, Brazil.

Surface urban heat islands (SUHIs) are one of the most studied phenomena in urban climates because they generate problems for the well-being of the urban population. This study analyzed the thermal comfort conditions at microclimate scale and SUHI for João Pessoa city, Brazil. Micrometeorological data (temperature and air humidity data) collected at 10 stations in 2011 and 2018 were used to calculate Thom's discomfort index (TDI) for João Pessoa city. Satellite images from Landsat 5/TM for 1991, 2006, and 2010 and Landsat 8/OLI for 2018 were used for land use and land cover classification and to identify SUHI. The obtained results highlighted that the SUHI area in João Pessoa city was 26 km2 and that almost half of the heat island area was concentrated in the Geisel, Aeroclube, Valentina, Distrito Industrial, Cristo Redentor, and Mangabeira neighborhoods. Regarding the micrometeorological data, higher values were obtained for 2018 in the dry periods (summer) and during the day. Based on the results, a considerable increase in discomfort during the daytime was observed in urbanized areas of the city from 2010 - 2018 due to the increase in the average temperature in João Pessoa between 1991 and 2018.

A influência de mídias multissensoriais na aprendizagem de crianças com transtorno de leitura / The influence of multisensory media on learning children with reading disorder

A dislexia é um transtorno específico de aprendizagem que pode afetar a habilidade de leitura prejudicando, principalmente, o processo de alfabetização. Na leitura, alguns dos sintomas observados são os erros de reconhecimento de palavras e a dificuldade de decodificação de palavras, o que acaba ocasionando um prejuízo na compreensão de textos. Pesquisas publicadas na literatura indicam que o uso de conteúdos multimídia melhoram o desempenho de leitura de escolares com dislexia. No entanto, a maioria das aplicações multimídia usam apenas dois dos cinco sentidos humanos: a visão e a audição. O MBook, livro multissensorial desenvolvido para este trabalho, utiliza um rastreador ocular para sincronizar mídias multissensoriais de vento, cheiro, sons e luzes ao texto que está sendo lido. Baseado na hipótese de que o livro multissensorial poderia diminuir a sobrecarga na memória de trabalho durante a leitura, favorecendo a compreensão de textos, aumentando o número de palavras lidas por minuto e aumentando a motivação pela leitura, foi realizada uma comparação entre dois estudos de caso individuais, um com um escolar com dislexia e outro sem. Os resultados apontam para um ganho maior na compreensão do texto e aumento na velocidade da leitura pelo escolar com dislexia ao utilizar mídias multissensoriais. Além disso, em ambos os casos, o uso da ferramenta indicou um aumento na motivação pela leitura.

Dyslexia is a specific learning disorder that can affect reading ability, mainly impairing the literacy process. In reading, some of the symptoms observed are errors of word recognition and difficulty in decoding words, which causes a loss in the comprehension of texts. Studies published in the literature indicate that the use of multimedia content improves the reading performance of students with dyslexia. However, most multimedia applications use only two of the five human senses: vision and hearing. The MBook, a multisensory book developed for this work, uses an eye tracker to synchronize wind, smell, sounds, and light effects to the text being read. Based on the hypothesis that the multisensory book could reduce the overload in working memory during reading, favoring the comprehension of texts, increasing the number of words read per minute and increasing motivation for reading, a comparison of two individual case studies was conducted: one with a student with dyslexia and one without. The results pointed to a greater gain in the comprehension of the text and an increase in reading speed by the student with dyslexia when multisensory media were used. In addition, with both students, the use of the tool pointed to an increase in reading motivation.

Sífilis materna: Análise de evidências referentes à falha no tratamento de gestantes / Maternal syphilis: Analysis of evidence regarding the failure to treat pregnant women

Objetivo: Analisar as evidências referentes à falha no tratamento da sífilis durante o período gestacional em mulheres atendidas em um hospital de referência em Parnaíba-PI. Metodologia: A amostra foi composta por 24 gestantes/puérperas admitidas no Hospital Estadual Dirceu Arcoverde para trabalho de parto e que apresentaram VDRL reagente durante a internação. Como instrumento de coleta de dados, utilizou-se um formulário estruturado. Após a coleta, os dados foram mensurados através de estatística descritiva. Resultados: Das 24 mulheres, 22 realizaram pré-natal e 12 apresentaram VDRL reagente. Entre as 12, 11 foram tratadas na gestação, mas somente 3 foram consideradas adequadamente tratadas. Conclusão: Uma assistência de pré-natal inadequada colabora para a ocorrência de falhas no tratamento de gestantes com sífilis, o que pode resultar no aumento do número de casos da forma congênita. Sendo assim, é necessário novas estratégias que visem reduzir a transmissão vertical da sífilis

Objective: To analyze the evidence regarding the failure to treat syphilis during the gestational period in women attending a referral hospital in Parnaíba-PI. Method: The sample consisted of 24 pregnant / puerperal women admitted to the Dirceu Arcoverde State Hospital for labor and who presented VDRL reagent during hospitalization. As a collection instrument, a structured form was used. After the data collection, the data were measured using descriptive statistics. Results: Of the 24 women, 22 underwent prenatal care and 12 presented VDRL reagent. Among the 12, 11 were treated in gestation, but only 3 were considered adequately treated. Conclusion: Inadequate prenatal care contributes to the occurrence of failures in the treatment of pregnant women with syphilis, which may result in an increase in the number of congenital cases. New strategies are needed to reduce vertical transmission of syphilis

Qualidade fisiológica de sementes de ingá em função da secagem / Quality seeds inga physiological function of drying

Entre os fatores decisivos para manter a qualidade das sementes está o teor de água, que quanto menor para algumas espécies maior será o tempo de manutenção da viabilidade dessas sementes, por outro lado há espécies cujas sementes são sensíveis a dessecação. Nesse sentido, o objetivo do presente trabalho foi avaliar a qualidade fisiológica de sementes de Inga laurina (Sw.) Willd. submetidas a dessecação. O experimento foi realizado no Centro de Ciências Agrárias da Universidade Federal da Paraíba (CCA-UFPB), em Areia - PB, em delineamento inteiramente ao acaso, com os tratamentos distribuídos num arranjo fatorial 2 x 8, perfazendo 16 tratamentos, constituídos pelas combinações de dois locais (casa de vegetação e ambiente laboratório) e oito períodos de secagem (0, 6, 12, 18, 24, 30, 36 e 42 horas), com quatro repetições de 25 sementes cada. Para avaliação do efeito da secagem determinou-se o teor de água, a germinação e o vigor (primeira contagem, índice de velocidade de germinação, comprimento e massa seca de raízes e parte aérea). As sementes de I. laurina podem ser secadas sem perda de viabilidade em ambiente natural de laboratório até 24 horas de secagem com valores médios de temperatura de 28,5 °C e umidade relativa do ar de 75 %.

Among the key factors to maintain seed quality is the water content, the lower that for some species the longer maintenance of the viability of the seed, on the other hand there are species whose seeds are sensitive to desiccation. Accordingly, the aim of this study was to evaluate the physiological quality of seeds of Inga laurina (Sw.) Willd. subjected to desiccation. The experiment was conducted at the Center for Agricultural Sciences, Federal University of Paraíba (CCA-UFPB) in Sand - PB, in a completely randomized design with treatments arranged in a factorial 2 x 8, totaling 16 treatments consisting of combinations of two locations (greenhouse and laboratory environment), and eight drying (0, 6, 12, 18, 24, 30, 36 and 42 hours), with four replicates of 25 seeds each. To evaluate the effect of drying was determined the water content, germination and vigor (first count, rate of germination, length and dry weight of roots and shoots). Seeds I. laurina can be dried without loss of viability in the natural environment of the laboratory within 24 hours of drying with average temperature of 28.5 ° C and relative humidity of 75%

Frequência de imunofenótipos aberrantes em leucemias agudas: análise de 213 casos diagnosticados na Fundação Hemope / Frequency of aberrant immunophenotypes in acute leukemia: analysis of 213 cases diagnosed in Fundação Hemope

As leucemias agudas (LAs) são doenças clonais do tecido hematopoético caracterizadas por proliferação anômala de progenitores das diferentes linhagens. 0 diagnóstico das LAs se baseia em achados citomorfológicos, citoquimicos e imunofenotipicos em células da Medula Óssea (MO) e/ou Sangue Periférico (SP). Cerca de 30 a 50% das LMAs e LLAs, bem caracterizadas pelo imunofenótipo, exibem expressão de antigenos aberrantes e esta situação deve ser distinguida das leucemias bifenotipicas, que tem atualmente critérios de diagnóstico bem definidos. A detecção dos antígenos aberrantes não parece ter implicação prognóstica, mas é uma importante ferramenta para a detecção de doença residual mínima (DRM). 0 objetivo deste trabalho foi relatar a frequência de Fenótipos Aberrantes (FA) em LAs nos pacientes diagnosticados na Fundação Hemope, correlacionar este achado com a idade e identificar os antígenos aberrantes predominantes. 0 estudo contou com 213 pacientes de ambos os sexos e sem restric;:ao de faixa etária ou raça. A imunofenotipagem utilizou amostras de SP e/ou MO, sendo a análise realizada por citometria de fluxo multiparamétrica. Para as LMAs, LLAs BeLLAs T as frequências de FA encontradas foram de 47%, 40% e 52%, respectivamente. Os antígenos aberrantes predominantes foram CD? e CD56 para as LMAs e CD13 e CD33 para as LLAs. A frequência de antígenos aberrantes (45%) e a predominância de antígenos linfocitários T e NK na LMAs e de antígenos mielóides nas LLAs, condizem com a literatura. Por outro lado, o predomínio de FA entre os adultos parece sugerir mais uma caracteristica da amostra, onde esta faixa etária predominou, que uma caracteristica biológica das leucemias analisadas. Finalmente, este estudo, definindo melhor o perfil imunofenotipico de nossos pacientes, possibilita o uso deste conhecimento na avaliação de DRM.

Acute leukemias (AL) are clonal diseases of the hematopoietic tissue characterized by anomalous proliferation of precursors of different lineage. The diagnosis of AL is based on morphological, citochemical and immunophenotypical findings in cells of Bone Marrow (BM) and/or Peripheral Blood (PB). About 30 to 50% of AML and ALL, well-characterized by immunophenotype, display aberrant expression of antigens and this situation should be distinguished from biphenotipic leukemias, which are currently well-defined criteria for diagnosis. The detection of aberrant antigen does not seem to have prognostic implication, but it is an important tool for the detection of minimal residual disease (MRD). The objective of this study was to report the frequency of aberrant phenotypes (AP) at AL in patients diagnosticated by Funda9iio Hemope, correlate this finding with age and identify the predominant aberrant antigens. The study included 213 patients of both genders and without restriction of age or race. The immunophenotyping used samples of PB and/or BM, being the analysis by multiparametric flow cytometry. For AML, B-ALL and T-ALL the frequencies of FA found were 47%, 40% and 52% respectively. The predominant aberrant antigens for AML were CD7 and CD56 and for ALL were CD33 and CD13. The frequency of aberrant antigens (45%) and the predominance of lymphocyte T and NK antigens in AML and myeloid antigens in ALL, matches with the literature. Moreover, the prevalence of AP among adults, who are the predominant age group, seems to be more suggestive of a characteristic of the sample, than a biological feature of leukemias that were analyzed. Finally, this study, defines better the immunophenotypical profile of our patients and allows the use of this knowledge in the evaluation.

Técnica de impressäo facial / Facial impression technique

A técnica de impressäo facial fornece ao técnico de laboratório referências tridimensionais essenciais de tecido mole, lábio, gengiva e rosto do paciente, auxiliando na obtençäo de uma prótese precisa e harmônica com a estrutura facial