Effects of topiramate, bupropion and naltrexone isolated or combined on subcutaneous adipose tissue in obese rats.

OBJECTIVE: To evaluate the effects of combining topiramate, bupropion and naltrexone in obesity-induced rats on their weight and subcutaneous adipose tissue. METHODS: A total of 40 male Wistar rats were induced to obesity for 8 weeks and the animals were divided into 8 groups: Ctr - control, G0 - Sham, G1 - oral saline solution (1.0mL/day), G2 - topiramate (20.0mg/kg) and bupropion (5.0mg/kg), G3 - naltrexone (20.0mg/kg), G4 - topiramate (20.0mg/kg), G5 - bupropion (5.0mg/kg) and G6 - topiramate (20.0mg/kg), bupropion (5.0mg/kg) and naltrexone (20.0mg/kg). During the experiment, all animals were weighed weekly. After 30 days of treatment animals were euthanized and their skin fragments were processed and stained with hematoxylin and eosin for morphological, morphometric and biochemical analyzes. RESULTS: The only group that presented a decrease in the volume of subcutaneous adipose tissue was G3, but this decrease was not significant when compared with the other groups. The G4, the G5 and the G6 presented increased adipose tissue volume. Data showed that until the eighth week all animals increased their weight by approximately 50%. After treatment animals of all groups, except G3, increased their weight from 4% to 9% approximately. The G3 was the only group that lost weight, but this decrease was not significant. CONCLUSION: The medicines studied were not efficient in reducing weight in obese rats. However, it should be considered that 30-day treatment period is not enough to observe the stronger effects of these drugs.

Effects of topiramate, bupropion and naltrexone isolated or combined on subcutaneous adipose tissue in obese rats

ABSTRACT Objective: To evaluate the effects of combining topiramate, bupropion and naltrexone in obesity-induced rats on their weight and subcutaneous adipose tissue. Methods: A total of 40 male Wistar rats were induced to obesity for 8 weeks and the animals were divided into 8 groups: Ctr - control, G0 - Sham, G1 - oral saline solution (1.0mL/day), G2 - topiramate (20.0mg/kg) and bupropion (5.0mg/kg), G3 - naltrexone (20.0mg/kg), G4 - topiramate (20.0mg/kg), G5 - bupropion (5.0mg/kg) and G6 - topiramate (20.0mg/kg), bupropion (5.0mg/kg) and naltrexone (20.0mg/kg). During the experiment, all animals were weighed weekly. After 30 days of treatment animals were euthanized and their skin fragments were processed and stained with hematoxylin and eosin for morphological, morphometric and biochemical analyzes. Results: The only group that presented a decrease in the volume of subcutaneous adipose tissue was G3, but this decrease was not significant when compared with the other groups. The G4, the G5 and the G6 presented increased adipose tissue volume. Data showed that until the eighth week all animals increased their weight by approximately 50%. After treatment animals of all groups, except G3, increased their weight from 4% to 9% approximately. The G3 was the only group that lost weight, but this decrease was not significant. Conclusion: The medicines studied were not efficient in reducing weight in obese rats. However, it should be considered that 30-day treatment period is not enough to observe the stronger effects of these drugs.

Reference values for biochemical parameters in Balb/c mice bred in the Faculdade de Medicina do Abc housing facilities, Santo André/SP, Brazil / Valores de referência de parâmetros bioquímicos de camundongos (Mus mus musculus) das linhagens Balb/c mantidos no biotério da Faculdade de Medicina do Abc, Santo André/SP, Brasil

The reference for biochemical parameters of laboratory animals, used in scientific research is based on the international literature. Comparing a few parameters evaluated in different studies with Balb/c mice, it was possible to observe that the reference values for these biochemical parameters vary enormously. It depends on several variables, such as sex, lineage and genotype, and are also influenced by other factors such as age, accommodation, animal handling, diet offered, sanitary conditions of the housing facilities, among others. The objective of this study is to establish a list of blood biochemical parameters of Balb/c mice (male and female) kept in the Housing facilities of Centro Universitário Saúde ABC. Thirty male and female animals weighing approximately 30g with 90 days of age were used. The animals were euthanized and blood was collected through caudal vena cava puncture, placed in collection tubes and the total cholesterol, triglycerides, transaminases (AST and ALT), urea, creatinine and glycemia parameters were determined. We did not observe a significant difference when comparing males and females in triglycerides, creatinine, urea and glycemia levels. However a significant difference was observed in the cholesterol, ALT and AST parameters. With these results, we were able to establish reference values for the Balb/c mice for comparison with future experiments, using a human semi-automatic analyzer.(AU)

A referência dos parâmetros bioquímicos dos animais de laboratório, utilizados para pesquisa científica é baseada na literatura internacional. Comparando-se alguns parâmetros avaliados em diferentes estudos com camundongos Balb/C pudemos observar que os valores de referência dos parâmetros bioquímicos variam enormemente, dependendo de diversas variáveis, como sexo, linhagem e genótipo e ainda sofrem influência de outros fatores como idade, condição de alojamento, manejo dos animais, dieta oferecida, padrão sanitário do biotério onde são mantidos, dentre outros. Desta forma o presente trabalho objetivou estabelecer uma lista de parâmetros bioquímicos do sangue de camundongos da linhagem Balb/C (machos e fêmeas) criados no Biotério da FMABC. Foram utilizados 30 animais machos e fêmeas, com peso +-30g e idade 90 dias. Os animais foram eutanasiados e o sangue foi coletado por meio da veia cava caudal, acondicionado em tubos de coleta e as dosagens de colesterol total, triglicérides, transaminases (AST e ALT), uréia, creatinina e glicemia foram analisados. Não observamos diferença significativa quando comparado machos e fêmeas em: Triglicérides, Creatinina, Uréia e Glicemia. Entretanto, observou-se diferença significativa nos parâmetros de colesterol, ALT e AST. Com estes resultados, pudemos então, estabelecer valores de referência para os camundongos Balb/C para comparação com futuros experimentos utilizando um analisador humano semi-automático.(AU)

Reference values for biochemical parameters in Balb/c mice bred in the Faculdade de Medicina do Abc housing facilities, Santo André/SP, Brazil / Valores de referência de parâmetros bioquímicos de camundongos (Mus mus musculus) das linhagens Balb/c mantidos no biotério da Faculdade de Medicina do Abc, Santo André/SP, Brasil

The reference for biochemical parameters of laboratory animals, used in scientific research is based on the international literature. Comparing a few parameters evaluated in different studies with Balb/c mice, it was possible to observe that the reference values for these biochemical parameters vary enormously. It depends on several variables, such as sex, lineage and genotype, and are also influenced by other factors such as age, accommodation, animal handling, diet offered, sanitary conditions of the housing facilities, among others. The objective of this study is to establish a list of blood biochemical parameters of Balb/c mice (male and female) kept in the Housing facilities of Centro Universitário Saúde ABC. Thirty male and female animals weighing approximately 30g with 90 days of age were used. The animals were euthanized and blood was collected through caudal vena cava puncture, placed in collection tubes and the total cholesterol, triglycerides, transaminases (AST and ALT), urea, creatinine and glycemia parameters were determined. We did not observe a significant difference when comparing males and females in triglycerides, creatinine, urea and glycemia levels. However a significant difference was observed in the cholesterol, ALT and AST parameters. With these results, we were able to establish reference values for the Balb/c mice for comparison with future experiments, using a human semi-automatic analyzer.

A referência dos parâmetros bioquímicos dos animais de laboratório, utilizados para pesquisa científica é baseada na literatura internacional. Comparando-se alguns parâmetros avaliados em diferentes estudos com camundongos Balb/C pudemos observar que os valores de referência dos parâmetros bioquímicos variam enormemente, dependendo de diversas variáveis, como sexo, linhagem e genótipo e ainda sofrem influência de outros fatores como idade, condição de alojamento, manejo dos animais, dieta oferecida, padrão sanitário do biotério onde são mantidos, dentre outros. Desta forma o presente trabalho objetivou estabelecer uma lista de parâmetros bioquímicos do sangue de camundongos da linhagem Balb/C (machos e fêmeas) criados no Biotério da FMABC. Foram utilizados 30 animais machos e fêmeas, com peso +-30g e idade 90 dias. Os animais foram eutanasiados e o sangue foi coletado por meio da veia cava caudal, acondicionado em tubos de coleta e as dosagens de colesterol total, triglicérides, transaminases (AST e ALT), uréia, creatinina e glicemia foram analisados. Não observamos diferença significativa quando comparado machos e fêmeas em: Triglicérides, Creatinina, Uréia e Glicemia. Entretanto, observou-se diferença significativa nos parâmetros de colesterol, ALT e AST. Com estes resultados, pudemos então, estabelecer valores de referência para os camundongos Balb/C para comparação com futuros experimentos utilizando um analisador humano semi-automático.