Lipidome remodeling in response to nutrient replenishment requires the tRNA modifier Deg1/Pus3 in yeast.

In the yeast Saccharomyces cerevisiae, the absence of the pseudouridine synthase Pus3/Deg1, which modifies tRNA positions 38 and 39, results in increased lipid droplet (LD) content and translational defects. In addition, starvation-like transcriptome alterations and induced protein aggregation were observed. In this study, we show that the deg1 mutant increases specific misreading errors. This could lead to altered expression of the main regulators of neutral lipid synthesis which are the acetyl-CoA carboxylase (Acc1), an enzyme that catalyzes a key step in fatty acid synthesis, and its regulator, the Snf1/AMPK kinase. We demonstrate that upregulation of the neutral lipid content of LD in the deg1 mutant is achieved by a mechanism operating in parallel to the known Snf1/AMPK kinase-dependent phosphoregulation of Acc1. While in wild-type cells removal of the regulatory phosphorylation site (Ser-1157) in Acc1 results in strong upregulation of triacylglycerol (TG), but not steryl esters (SE), the deg1 mutation more specifically upregulates SE levels. In order to elucidate if other lipid species are affected, we compared the lipidomes of wild type and deg1 mutants, revealing multiple altered lipid species. In particular, in the exponential phase of growth, the deg1 mutant shows a reduction in the pool of phospholipids, indicating a compromised capacity to mobilize acyl-CoA from storage lipids. We conclude that Deg1 plays a key role in the coordination of lipid storage and mobilization, which in turn influences lipid homeostasis. The lipidomic effects in the deg1 mutant may be indirect outcomes of the activation of various stress responses resulting from protein aggregation.

Tecnologias aplicadas pela enfermagem no cuidado neonatal / Technology applied for newborn care in nursing / Tecnología aplicada para el cuidado del recién nascido em enfermaria

Trata-se de pesquisa de abordagem qualitativa realizada com o objetivo de identificar as tecnologias do cuidado empregadas em Unidades de Terapia Intensiva Neonatal (UTIN) de Hospitais Universitários Federais da região Sudeste do Brasil. A entrevista foi utilizada na coleta de dados realizada no período de junho a agosto de 2011. Os resultados, apresentados em quatro categorias, ressaltaram que a transformação do cuidado dirigido ao recém- -nascido evoluiu em duas vertentes que se complementam: o avanço de tecnologias duras e o avanço de tecnologias leves. Ambas são imprescindí-veis ao cuidado neonatal, mas a última, em especial, ainda se constitui num desafio para a enfermagem neonatal. Concluiu-se que já se caminha bem no desenvolvimento de tecnologias que proporcionem a sobrevivência de recém-nascidos considerados até pouco tempo incompatíveis com a vida, mas persiste o desafio de desenvolver tecnologias que deem vida aos dias desses recém-nascidos.

A research with a qualitative approach performed with the purpose of identifying healthcare technologies used in Neonatal Intensive Care Units (NICU) in Federal University Hospitals of the southeastern region of Brazil. The interview was used in data collection during the period of June to August 2012. The results, presented in four categories, emphasized that the transformation of healthcare guided to newborns evolved in two mutually complementary aspects: the advance of hard technologies and the advance of soft technologies. Both are indispensable to neonatal care, nevertheless the latter is still a challenge for neonatal nursing care. It was concluded that the development of technologies for providing survival for newborns, previously considered incompatible with life, is well on track, but the challenge persists for the development of technologies that bring life to the days of these newborns.

Se trata de una pesquisa de abordaje cualitativa realizada con el objetivo de identificar las tecnologí-as del cuidado empleado en Unidades de Terapia Intensiva Neonatal (UTIN) de Hospitales Univer-sitarios Federales de la región sudeste de Brasil. La entrevista fue utilizada en la colecta de datos realizada en el periodo de junio a agosto de 2012. Los resultados, presentados en cuatro categorías, resaltaron que la transformación del cuidado dirigido al recién-nascido evolucionó en dos vertientes que se complementan: avanzo de tecnologías duras y el avanzo de tecnologías leves. Ambas son imprescindibles al cuidado neonatal, pero la última, en especial, aún se constituye en un desafío para la enfermería neonatal. Se concluye que ya se camina bien en el desarrollo de tecnologías que proporcionen la sobrevivencia de recién-nascidos considerados hasta poco tiempo incompatibles con la vida, pero persiste el desafío de desarrollar tecnologías que den vida a los días de esto recién-nascidos.