Reactive oxygen species impair Na+ transport and renal components of the renin-angiotensin-aldosterone system after paraquat poisoning.

Paraquat (1,1'-dimethyl-4,4'-bipyridyl dichloride) is an herbicide widely used worldwide and officially banned in Brazil in 2020. Kidney lesions frequently occur, leading to acute kidney injury (AKI) due to exacerbated reactive O2 species (ROS) production. However, the consequences of ROS exposure on ionic transport and the regulator local renin-angiotensin-aldosterone system (RAAS) still need to be elucidated at a molecular level. This study evaluated how ROS acutely influences Na+-transporting ATPases and the renal RAAS. Adult male Wistar rats received paraquat (20 mg/kg; ip). After 24 h, we observed body weight loss and elevation of urinary flow and serum creatinine. In the renal cortex, paraquat increased ROS levels, NADPH oxidase and (Na++K+)ATPase activities, angiotensin II-type 1 receptors, tumor necrosis factor-α (TNF-α), and interleukin-6. In the medulla, paraquat increased ROS levels and NADPH oxidase activity but inhibited (Na++K+)ATPase. Paraquat induced opposite effects on the ouabain-resistant Na+-ATPase in the cortex (decrease) and medulla (increase). These alterations, except for increased serum creatinine and renal levels of TNF-α and interleukin-6, were prevented by 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (tempol; 1 mmol/L in drinking water), a stable antioxidant. In summary, after paraquat poisoning, ROS production culminated with impaired medullary function, urinary fluid loss, and disruption of Na+-transporting ATPases and angiotensin II signaling.

Periapical Lesions Associated with Demographic Variables, Dental Conditions, Systemic Diseases, and Habits.

AIM AND BACKGROUND: The aim of this study was to analyze the prevalence of periapical lesions and possible associations with demographic, dental conditions, systemic diseases, and habit variables in patients from a private Brazilian university. MATERIALS AND METHODS: Data from 452 patients of both sexes, aged 18-78 years, from a Brazilian university were evaluated. Panoramic radiographs were analyzed, and the presence of periapical lesions was recorded. In these cases, the dental condition was assessed for the presence or absence of endodontic treatment. Medical records provide information related to general health (diabetes, cardiovascular diseases, altered cholesterol, autoimmune diseases, gallstones, or kidney stones) and habits (smoking or alcoholism). The data were descriptively analyzed, and then logistic regression and the Wald test were performed in Stata/SE v.14.1. RESULTS: A rate of 58.4% of participants were women, and the mean age was 36.4 (±14.6) years. Periapical lesions prevailed in 193 (42.7%) patients, and 281 (72.4%) teeth were not previously endodontically treated. Mandibular first molars (19%) and men (48.9%) were the most affected. The adjusted model showed that the age group of 26-45 years was 5 times more likely [odds ratio (OR) = 5.01; 95% confidence interval (CI): 2.85-8.82] to have lesions than those aged ≤25 years. Participants aged above 46 years were 19.1 times more prone (OR = 19.1; 95% CI: 10.2-36.0) to morbidity than younger ones (≤25 years). CONCLUSION: The studied sample showed that periapical lesions were more prevalent in males, in mandibular molars, and without prior endodontic treatment. There was no observed association between periapical lesions and comorbidities or habits; however, a significant correlation occurred with advancing age. CLINICAL SIGNIFICANCE: Comorbidities and habits were not associated with the presence of periapical lesions. However, aging has been identified as a significant risk factor for the development of periapical lesions. This finding is clinically relevant as it highlights the importance of monitoring and maintaining oral health in patients with advanced age. How to cite this article: dos Santos VC, de Oliveira Kublitski PM, Marques da Silva B, et al. Periapical Lesions Associated with Demographic Variables, Dental Conditions, Systemic Diseases, and Habits. J Contemp Dent Pract 2023;24(11):864-870.

O uso de vídeos para a implementação de protocolos de reabilitação entre a equipe multiprofissional durante o manejo de pacientes com doença de Parkinson: uma revisão integrativa / The use of videos for the implementation of rehabilitation protocols between the multiprofessional team during the management of patients with Parkinson's disease: an integrative review / El uso de videos para la aplicación de protocolos de rehabilitación entre el equipo multiprofesional durante el manejo de pacientes con enfermedad de Parkinson: una revisión integrativa

Objetivo: Avaliar a efetividade do uso de vídeos para a implementação de protocolos de reabilitação entre a equipe multiprofissional durante o manejo dos pacientes com Doença de Parkinson. Métodos: Foi conduzida uma revisão integrativa da literatura a partir da pergunta norteadora, usando os elementos do PICO para identificar os descritores de assunto e sinônimos para as bases de dados Cochrane Library (CENTRAL), LILACS, PubMed, Education Resources Information Center (ERIC). Foi planejado incluir estudos que avaliaram a ferramenta de vídeo como estratégia de desenvolvimento da equipe multiprofissional que atua na reabilitação do paciente com Doença de Parkinson, independentemente do ano e do idioma de publicação. A avaliação crítica da literatura também foi planejada para todos os estudos incluídos. Resultados: Nenhum estudo atendeu ao critério de inclusão desta revisão e por isso os autores decidiram discutir e relatar uma síntese dos estudos semelhantes, com potencial para a inclusão, do tema abordado na pergunta norteadora. Assim, um total de dez estudos foram sintetizados em quadros. Conclusão: Não foi possível identificar a efetividade da intervenção estudada devido à ausência de estudos clínicos com essa finalidade. Entretanto, foram avaliados estudos semelhantes para aprimorar o delineamento de um possível estudo clínico.

Objective: To evaluate the effectiveness of using videos for the implementation of rehabilitation protocols among the multidisciplinary team during the management of patients with Parkinson's disease. Methods: An integrative literature review was conducted based on the guiding question, using PICO elements to identify subject descriptors and synonyms for the Cochrane Library (CENTRAL), LILACS, PubMed, Education Resources Information Center) databases. It was planned to include studies that evaluated the video tool as a development strategy for the multidisciplinary team that works in the rehabilitation of patients with Parkinson's disease, regardless of the year and language of publication. Critical literature review was also planned for all included studies. Results: No study met the inclusion criteria of this review and therefore the authors decided to discuss and report a synthesis of similar studies, with potential for inclusion, of the topic addressed in the guiding question. Thus, a total of ten studies were summarized in tables. Conclusion: It was not possible to identify the effectiveness of the intervention studied due to the lack of clinical studies with this purpose. However, similar studies were evaluated to improve the design of a possible clinical study.

Objetivo: Evaluar la efectividad del uso de videos para la implementación de protocolos de rehabilitación entre el equipo multidisciplinario durante el manejo de pacientes con enfermedad de Parkinson. Métodos: Se realizó una revisión integrativa de la literatura basada en la pregunta guía, utilizando elementos PICO para identificar descriptores para las bases de datos Cochrane Library (CENTRAL), LILACS, PubMed, Education Resources Information Center (ERIC). Se planeó incluir estudios que evaluaran la herramienta de video como estrategia de desarrollo para el equipo multidisciplinario que trabaja en la rehabilitación de pacientes con enfermedad de Parkinson, independientemente del año e idioma de publicación. También se planeó una revisión crítica de la literatura para todos los estudios incluidos. Resultados: Ningún estudio cumplió con los criterios de inclusión de esta revisión y, por lo tanto, los autores decidieron discutir e informar una síntesis de estudios similares, con potencial de inclusión, del tema abordado en la pregunta guía. Por lo tanto, un total de diez estudios se resumieron en tablas. Conclusión: No fue posible identificar la efectividad de la intervención estudiada debido a la falta de estudios clínicos con este fin. Sin embargo, se evaluaron estudios similares para mejorar el diseño de un posible estudio clínico.

Erros de prescrição, dose, preparo e desperdício de medicamentos relacionados a distribuição individualizada de antimicrobiano para pacientes de uti-neonatal / Prescription errors, dose, preparation and drug wastage related to individualized antimicrobial distribution for uti-neonatal patients / Errores de prescripción, dosis, preparación y desperdicio de medicamentos relacionados con la distribución individualizada de antimicrobianos a pacientes en unidades de cuidados intensivos neonatales

Introdução: Dentre as diversas áreas de sua competência, a Farmácia Hospitalar é responsável pela promoção e monitoramento do uso seguro de medicamentos. Objetivo: Investigar erros de prescrição, de dose, de preparo e desperdício de antimicrobianos distribuídos de forma individualizada para pacientes de UTI-Neonatal na Fundação Santa Casa de Misericórdia do Pará. Metodologia: Trata-se de uma pesquisa transversal, retrospectiva, fundamentada na pesquisa-ação, tendo como recorte temporal o período de novembro de 2021 a janeiro 2022. As variáveis relacionadas aos medicamentos foram comparadas ao protocolo de prescrição do NEOFAX®. Resultado: O estudo envolveu 76 pacientes e analisou 213 prescrições que envolveram 341 antimicrobianos, dos quais, 280 (82%) dos antimicrobianos prescritos não apresentavam clareza quanto às instruções de preparo e 98 (29%) apresentaram as doses em desacordo com o NEOFAX®. Os itens de maior dúvida foram quanto à estabilidade após o preparo: 341 (100%); quanto ao volume final após preparo 341 (100%) e quanto a técnica de preparo 266 (78%). Do total de pacientes, 32 (42%) eram de muito baixo peso, seguidos de 26 (34%) de extremo baixo peso, e 18 (24%) de baixo peso. Conclusão: Os dados obtidos no estudo subsidiaram a implantação de uma central de misturas intravenosas na instituição estudada.

Introduction: Among the various areas of its competence, the Hospital Pharmacy is responsible for promoting and monitoring the safe use of medicines. Objective: To investigate errors in prescribing, dosage, preparation and waste of antimicrobials distributed individually to neonatal ICU patients at Santa Casa de Misericórdia Foundation of Pará. Methodology: This is a retrospective cross-sectional study, based on action research, with a time frame of November 2021 to January 2022. The variables related to medications were compared to the NEOFAX® prescription protocol. Results: The study involved 76 patients and analyzed 213 prescriptions involving 341 antimicrobials. Of these, 280 (82%) of the prescribed antimicrobials were unclear regarding preparation instructions and 98 (29%) had doses in disagreement with NEOFAX®. The most doubtful items were about stability after preparation: 341 (100%); about the final volume after preparation 341 (100%) and about the preparation technique 266 (78%). Of the total number of patients, 32 (42%) were very low weight, followed by 26 (34%) extreme low weight, and 18 (24%) low weight. Conclusion: The data obtained in this study supported the implementation of an IV mixing center at the studied institution.

Introducción: Entre varias áreas de su competencia, la Farmacia Hospitalaria es responsable de promover y vigilar el uso seguro de los medicamentos. Objetivo: Investigar errores de prescripción, dosificación, preparación y desperdicio de antimicrobianos distribuidos de forma individualizada para pacientes de UCI neonatal en la Fundación Santa Casa de Misericórdia de Pará. Metodologia: Trata-se de uma pesquisa transversal, retrospectiva, fundamentada na pesquisa-ação, tendo como recorte temporal o período de novembro de 2021 a janeiro 2022. Las variables relacionadas con la medicación se compararon con el protocolo de prescripción NEOFAX®. Resultados: El estudio involucró 76 pacientes y analizó 213 prescripciones que involucraron 341 antimicrobianos, de los cuales, 280 (82%) de los antimicrobianos prescritos no eran claros en cuanto a las instrucciones de preparación y 98 (29%) presentaban las dosis en desacuerdo con el NEOFAX®. Los ítems de mayor duda fueron sobre la estabilidad tras la preparación: 341 (100%); sobre el volumen final tras la preparación 341 (100%) y sobre la técnica de preparación 266 (78%). Del total de pacientes, 32 (42%) eran de muy bajo peso, seguidos de 26 (34%) de bajo peso extremo y 18 (24%) de bajo peso. Conclusión: Los datos obtenidos en el estudio subsidian la implementación de un centro de mezclas intravenosas en la institución estudiada.

A importância das mulheres negras na enfermagem do brasil e do mundo / The importance of black women in nursing in brazil and the world / La importancia de las mujeres negras en la enfermería en brasil y en el mundo

Objetivo: compreender a importância das mulheres negras na enfermagem e sua representatividade. Método: trata-se de uma revisão historiográfica realizada através de um levantamento em base de dados eletrônicos, que foram capturados por meio dos descritores em Ciência da Saúde: "História da Enfermagem", "Cuidados de Enfermagem", "Enfermagem em Saúde Comunitária", "Biografia" e "Racismo", combinados pelo operador bolleano "and", nos sites da Biblioteca Virtual em Saúde, da Scientific Electronic Library Online. Também foram utilizados documentos da literatura cinzenta e sites de domínio público. Resultados: foram avaliados 54 documentos relevantes ao estudo e que atenderam aos critérios de inclusão, sendo possível sistematizar e categorizar 13 mulheres negras que se destacaram na enfermagem e os seus principais feitos. Elas são: Mary Jane Seacole, Mary Eliza Mahoney, Mary Elizabeth Carnegie, Clara Adams Ender, Maria José Barroso ("Maria Soldado"), Maria Barbosa Fernandes, Lydia das Dores Matta, Maria de Lourdes Almeida, Josephine de Mello, Rosalda Paim, Izabel dos Santos, Maria Estella de Azevedo dos Santos e Ivone Lara. Conclusão: o negro, sobretudo as mulheres negras, sempre esteve ativamente envolvido no processo de cuidar, em todas as suas formas, porém seu protagonismo foi esquecido e invisibilizado, portanto, quanto mais se resgata fatos históricos e se coloca em evidência marcos que contribuíram para a mudança de paradigmas, menos riscos se corre em reproduzir e perpetuar histórias com protagonistas únicos. Descritores: História da Enfermagem; Cuidados de Enfermagem; Enfermagem em Saúde Comunitária; Biografia; Racismo..

Objective: to understand the importance of black women in nursing and its representativeness. Method: this is a historiographical review carried out through a survey based on electronic databases, which were captured through the descriptors in Health Science: "History of Nursing", "Nursing Care", "Community Health Nursing", "Biography" and "Racism", combined by the Bollean operator "and", on the websites of the Virtual Health Library, of the Scientific Electronic Library Online. Documents from grey literature and public domain sites were also used. Results: 54 documents relevant to the study were evaluated and met the inclusion criteria, being possible to systematize and categorize 13 black women who stood out in nursing and their main achievements. They are: Mary Jane Seacole, Mary Eliza Mahoney, Mary Elizabeth Carnegie, Clara Adams Ender, Maria José Barroso ("Maria Soldado"), Maria Barbosa Fernandes, Lydia das Dores Matta, Maria de Lourdes Almeida, Josephine de Mello, Rosalda Paim, Izabel dos Santos, Maria Estella de Azevedo dos Santos and Ivone Lara. Conclusion: black people, especially black women, have always been actively involved in the care process, in all its forms, but its protagonism has been forgotten and invisible, therefore, the more historical facts are rescued and milestones that have contributed to the paradigm shift are highlighted, the less risks are in reproducing and perpetuating stories with unique protagonists. Descriptors: History of Nursing; Nursing Care; Community Health Nursing; Biography; Racism.

Objetivo: comprender la importancia de las mujeres negras en enfermería y su representatividad. Método: se trata de una revisión historiográfica realizado a través de una encuesta basada en bases de datos electrónicas, que fueron capturadas a través de los descriptores en Ciencias de la Salud: "Historia de la Enfermería", "Atención de Enfermería", "Enfermería en Salud Comunitaria", "Biografía" y "Racismo", combinados por el operador Bollean "y", en los sitios web de la Biblioteca Virtual en Salud, de la Biblioteca Electrónica Científica en Línea. También se utilizaron documentos de literatura gris y sitios de dominio público. Resultados: 54 documentos relevantes para el estudio fueron evaluados y cumplieron con los criterios de inclusión, siendo posible sistematizar y categorizar a 13 mujeres negras que se destacaron en enfermería y sus principales logros. Ellos son: Mary Jane Seacole, Mary Eliza Mahoney, Mary Elizabeth Carnegie, Clara Adams Ender, Maria José Barroso ("Maria Soldado"), Maria Barbosa Fernandes, Lydia das Dores Matta, Maria de Lourdes Almeida, Josephine de Mello, Rosalda Paim, Izabel dos Santos, Maria Estella de Azevedo dos Santos e Ivone Lara. Conclusión: las personas negras, especialmente las mujeres negras, siempre han participado activamente en el proceso de cuidado, en todas sus formas, pero su protagonismo ha sido olvidado e invisible, por lo tanto, mientras más hechos históricos se rescatan y se destacan los hitos que han contribuido al cambio de paradigma, menos riesgos hay en reproducir y perpetuar historias con protagonistas únicos. Descriptores: Historia de la Enfermería; Atención de Enfermería; Enfermería em Salud Comunitaria; Biografía; Racismo

Phylogenetic relationships of the supercontig of sodium channel subunit I (NaV) in 17 species of Anopheles (Diptera: Culicidae).

BACKGROUND: Malaria is a global health problem and is transmitted by the Anopheles species. Due to the epidemiological importance of the genus, studies on biological, phylogenetic, and evolutionary aspects have contributed to the understanding of adaptation, vector capacity, and resistance to insecticides. The latter may result from different causes such as mutations in the gene that encodes the sodium channel (NaV). METHODS: In this study, the NaV subunit I scaffold of 17 anopheline species was used to infer phylogenetic relationships of the genus Anopheles using Bayesian inference. The evolutionary phylogenetic tree of the NaV gene was aligned in the AliView program and analyzed utilizing Bayesian inference, using the software MrBayes. RESULTS: The anophelines were grouped into five well-supported clusters: 1 - Anopheles darlingi and Anopheles albimanus; 2 - Anopheles sinensis and Anopheles atroparvus; 3 - Anopheles dirus; 4 - Anopheles minimus, Anopheles culicifacies, Anopheles funestus, Anopheles maculatus, and Anopheles stephensi; and 5 - Anopheles christyi, Anopheles epiroticus, Anopheles merus, Anopheles melas, Anopheles gambiae, Anopheles coluzzii, and Anopheles arabiensis. CONCLUSIONS: The topology confirms the phylogenetic relationships proposed in studies based on the genome of some anophelines and reflects the current taxonomy of the genus, which suggests that NaV undergoes selection pressure during the evolution of the species. These data are useful tools for inferring their ability to resist insecticides and also help in better understanding the evolutionary processes of the genus Anopheles.

Conhecimento de leigos sobre os primeiros socorros no ambiente extra-hospitalar / Conocimiento laico sobre primeros auxilios en el entorno extrahospitalario / Lay knowledge about first aid in the extra-hospital environment

Objetivo: Avaliar o conhecimento da população leiga sobre primeiros socorros no ambiente extra-hospitalar. Método: estudo descritivo, exploratório e quantitativo, aprovado pelo Comitê de Ética sob protocolo CAAE no47763121.4.0000.5587, realizado com uma população leiga na região Norte de Mato Grosso, avaliando 150 pessoas, utilizando um questionário validado. Resultados: dos participantes; cerca de 78% souberam identificar os sinais vitais, 58% hemorragia interna e 55% hemorragia externa e 55,3% chamam o socorro após essa verificação. Em relação a queimaduras 65,3% acertaram quanto aos cuidados. Em situações de envenenamento 47% sabem que o paciente deve ser levado para emergência, e 11% sabem avaliar os riscos. Quanto ao conhecimento de realizar uma massagem cardíaca, 54% desconhecem o local correto e compressões. Em relação a prestar os primeiros socorros 84,6% não estão preparados, e 72,6% descrevem inaptos, déficit de capacitação. Conclusão: A população leiga possui conhecimento ainda precário, sugerindo cursos de primeiros socorros.(AU)

Objective: To assess the lay population's knowledge about first aid in the out-of-hospital environment. Critical, exploratory study and published protocol CAAE no47763121.587, carried out with a law in the northern region of Mato Grosso, evaluating 150 people, using the valid one method. Results: from the participants; about 78% of internal protection after the help, 58% of hemorrhage and 55.3% call this verification help. In relation to respect, 65.3% were right about care. In poisoning situations, 47% know that the patient should be taken to an emergency room, and 11% know how to assess the risks. As for the knowledge of performing a cardiac massage, 54% are unaware of the correct location and compressions. Regarding the beginning of first aid, 4.6% are not prepared, and 72.6% describe unfit, capacity deficit. Conclusion: The lay population still has precarious knowledge, suggesting first aid courses.(AU)

Objetivo: Evaluar el conocimiento de la población general sobre primeros auxilios en el medio extrahospitalario. Estudio crítico, exploratorio y protocolo publicado CAAE no47763121.587, realizado con una ley en la región norte de Mato Grosso, evaluando 150 personas, utilizando el método válido. Resultados: de los participantes; alrededor del 78% de protección interna después de la ayuda, el 58% de hemorragia y el 55,3% llaman a esta verificación ayuda. En relación al respeto, el 65,3% acertó sobre el cuidado. En situaciones de intoxicación, el 47% sabe que el paciente debe ser llevado a urgencias y el 11% sabe evaluar los riesgos. En cuanto al conocimiento de realizar un masaje cardíaco, el 54% desconoce la ubicación correcta y las compresiones. En cuanto al inicio de los primeros auxilios, el 4,6% no está preparado y el 72,6% describe no apto, déficit de capacidad. Conclusión: La población laica aún tiene conocimientos precarios, sugiriendo cursos de primeros auxilios.(AU)

Política Nacional de Educação Permanente em Saúde: aspectos que compõem o perfil dos profissionais que atuam nas Comissões de Integração Ensino-Serviço (CIES) Regionais no Pará / National Policy on Permanent Education in Health: aspects that make up the profile of professionals who work in the Regional Teaching-Service Integration Commissions (CIES) in Pará

Este artigo propõe analisar aspectos que compõem o perfil dos profissionais que atuam nas Comissões de Integração Ensino Serviço (CIES) nas Regiões de Saúde no Pará, considerando a importância do funcionamento dessas comissões para o avanço da PNEPS no estado. Tratou-se de uma pesquisa do tipo estudo de caso, com análise qualitativa de carácter exploratório. Os sujeitos pesquisados foram os 25 profissionais que atuavam na Educação Permanente em Saúde na SESPA com atividades direcionadas às CIES Regionais, nos 13 Centros Regionais de Saúde. Foram utilizados questionários auto aplicáveis no formato online. Os achados evidenciaram elevada adesão dos participantes à pesquisa; o tempo médio de atuação na CIES Regional é de 2 anos; identificou-se elevado grau de escolaridade; a maioria faz parte da categoria de profissionais de saúde; todos participantes informaram ter vínculo estável e atribuíram grau alto de importância para o funcionamento das CIES. Concluiu-se a necessidade de continuação de pesquisas nessa área, contudo, o presente estudo propôs colocar em análise os aspectos do perfil desses profissionais para somar esforços com informações que possa contribuir com as esferas de gestão do estado para o desenvolvimento de medidas que estejam alinhadas a consolidação da PNEPS enquanto política pública(AU)

This article proposes to analyze aspects that make up the profile of professionals who work in the Education Service Integration Commissions (CIES) in the Health Regions in Pará, considering the importance of the functioning of these commissions for the advancement of PNEPS in the state. It was a case study research, with qualitative analysis of an exploratory nature. The research subjects were the 25 professionals who worked in Permanent Education in Health at SESPA with activities directed to the Regional CIES, in the 13 Regional Health Centers. Self-administered online questionnaires were used. The findings showed high adherence of participants to the research; the average time working at CIES Regional is 2 years; a high level of schooling was identified; the majority are part of the category of health professionals; all participants reported having a stable relationship and attributed a high degree of importance to the functioning of the CIES. The need for further research in this area was concluded, however, the present study proposed to analyze the aspects of the profile of these professionals to add efforts with information that can contribute to the spheres of state management for the development of measures that are aligned the consolidation of PNEPS as a public policy (AU)

Este artículo se propone analizar aspectos que componen el perfil de los profesionales que actúan en las Comisiones de Integración de los Servicios de Educación (CIES) en las Regiones de Salud de Pará, considerando la importancia del funcionamiento de estas comisiones para el avance de la PNEPS en el estado. Se trató de una investigación de estudio de caso, con análisis cualitativo de carácter exploratorio. Los sujetos de la investigación fueron los 25 profesionales que actuaban en la Educación Permanente en Salud de la SESPA con actividades dirigidas al CIES Regional, en los 13 Centros Regionales de Salud. Se utilizaron cuestionarios en línea autoadministrados. Los hallazgos mostraron alta adherencia de los participantes a la investigación; el tiempo promedio de trabajo en el CIES Regional es de 2 años; se identificó un alto nivel de escolaridad; la mayoría forman parte de la categoría de profesionales de la salud; todos los participantes informaron tener una relación estable y atribuyeron un alto grado de importancia al funcionamiento del CIES. Se concluyó la necesidad de más investigaciones en esta área, sin embargo, el presente estudio se propuso analizar los aspectos del perfil de estos profesionales para sumar esfuerzos com informaciones que puedan contribuir a los ámbitos de la gestión estatal para el desarrollo de medidas que estén alineadas con la consolidación del PNEPS como política pública

Tuberculosis en tiempos de COVID-19: situación de la tuberculosis pediátrica en Uruguay en 2020 / Tuberculosis in times of COVID-19: pediatric tuberculosis in Uruguay in 2020 / Tuberculose em tempos de COVID-19: situação da tuberculose pediátrica no Uruguai em 2020

Introducción: la Organización Mundial de la Salud (OMS) alertó sobre el riesgo de descuidar acciones de control de la tuberculosis (TB) durante la pandemia de COVID-19. Objetivos: describir la situación de la TB en menores de 15 años en Uruguay en 2020, y compararla con 2019. Describir estrategias del Programa Nacional de Tuberculosis (PNT) para mitigar el impacto de la pandemia de COVID-19. Metodología: Estudio descriptivo, retrospectivo de menores de 15 años con TB en Uruguay entre 1/1/2020 y 31/12/2020. Datos patronímicos, diagnóstico clínico y bacteriológico, tratamiento y estudio de contactos; indicadores epidemiológicos de 2019. Se calculó tasa de incidencia, frecuencias y porcentajes. Se seleccionaron estrategias del PNT dirigidas o que impactan directamente en la atención pediátrica. Resultados: en 2020, se registraron 61 casos de TB en menores de 15 años. Tasa de incidencia 8,8/100.000. Presentaron TB pediátrica 6,3% del total de casos (61/968), y en 2019 3,9% (42/1057). Formas pulmonares 84% y extrapulmonares 16%. Confirmación bacteriológica: 25%. No hubo fallecidos en 2020 y hubo uno en 2019. Contactos: en 2020 disminuyó 11% la notificación; se estudió al 86%, se indicó quimioprofilaxis al 73%. Aumento significativo de contactos en menores 15 años enfermos (2,4% 2019 y 5% 2020). Estrategias del PNT: 1) Diagnóstico: uso de GeneXpert Ultra; 2) Tratamiento: supervisión por video (VOT); 3) Contactos: se priorizó a menores de 15 años adecuando protocolo de estudio. Conclusiones: en 2020 se mantuvieron las acciones de control de la TB en la población pediátrica. Aumentó la incidencia de TB en menores de 15 años. Los niños y adolescentes fueron priorizados en el diagnóstico (técnicas más sensibles) y estudio de contactos.

Summary: Introduction: the WHO warned about the risk of neglecting tuberculosis (TB) control actions during the COVID-19 pandemic. Objectives: describe the TB situation in children <15 years of age in Uruguay in 2020, and compare it with 2019. Describe strategies of the National Tuberculosis Program (NTP) to mitigate the impact of the COVID 19 pandemic. Material and methods: descriptive, retrospective study of children <15 years of age with TB in Uruguay 1/1/2020 - 12/31/2020. Patronymic data, clinical and bacteriological diagnosis, treatment and study of contacts; epidemiological indicators of 2019. Incidence rate, frequency and percentages were calculated. NTP strategies aimed at or directly impacting pediatric care were selected. Results: in 2020, 61 cases of TB in children < 15 years. Incidence rate 8.8/100,000. Pediatric TB 6.3% of total cases (61/968), and in 2019 3.9% (42/1057). Pulmonary forms 84% and extrapulmonary 16%. Bacteriological confirmation: 25%. No deaths in 2020 and 1 in 2019. Contacts: in 2020 the notification decreased by 11%; 86% were studied, 73% chemoprophylaxis. Significant increase in infected contacts < 15(2.4% 2019 and 5% 2020). NTP strategies: 1- Diagnosis: use of GeneXpert Ultra; 2- Treatment: video surveillance (VOT); 3- Contacts: children of <15 years were prioritized, adapting the study protocol. Conclusions: in 2020, the TB control actions were maintained in the pediatric population. The incidence of TB increased in children <15 years of age. Children and adolescents were prioritized in diagnosis (more sensitive techniques) and contact studies.

Introdução: a OMS alertou sobre o risco de negligenciar as ações de controle da tuberculose (TB) durante a pandemia de COVID-19. Objetivos: descrever a situação da TB em <15 anos no Uruguai 2020 e comparar com 2019. Descrever as estratégias do Programa Nacional de Tuberculose (PNT) para mitigar o impacto da pandemia de COVID-19. Metodologia: estudo descritivo retrospectivo de <15 anos com TB no Uruguai 01/01/2020 - 31/12/2020. Dados patronímicos, diagnóstico clínico e bacteriológico, tratamento e estudo de contatos; indicadores epidemiológicos de 2019. Calculou-se a taxa de incidência, frequência e percentuais. Estratégias de PNT destinadas ou impactando diretamente os cuidados pediátricos foram selecionadas Resultados: em 2020, 61 casos de TB em crianças <15 anos. Taxa de incidência 8,8/100.000. TB pediátrica 6,3% do total de casos (61/968), e em 2019 3,9% (42/1057). Formas pulmonares 84% e extrapulmonares 16%. Confirmação bacteriológica: 25%. Não houve falecidos em 2020 e 1 em 2019. Contatos: em 2020 a notificação diminuiu 11%; 86% foram estudados, 73% quimioprofilaxia. Aumento significativo de contatos infetados < 15 pacientes (2,4% 2019 e 5% 2020). Estratégias de NTP: 1- Diagnóstico: uso do GeneXpert Ultra; 2- Tratamento: supervisão por vídeo (VOT); 3- Contatos: <15 anos foram priorizados, adaptando o protocolo do estudo. Conclusões: em 2020, as ações de controle da TB foram mantidas na população pediátrica. A incidência de TB aumentou em <15 anos. Crianças e adolescentes foram priorizados no diagnóstico (técnicas mais sensíveis) e no estudo de contatos.

Territórios tradicionais de vida e as zonas de sacrifício do agronegócio no Cerrado / Traditional life territories and the agribusiness sacrifice zone in the Cerrado

RESUMO A imensa sociobiodiversidade do Cerrado brasileiro pode ser compreendida a partir dos modos de vida construídos pelo amplo leque de povos e comunidades tradicionais em suas relações com o bioma, do qual são guardiãs. Nas últimas décadas, projetos de desenvolvimento promovem ali acelerado avanço do agronegócio, expropriando terras, privatizando águas, contaminando o ambiente e ameaçando ou inviabilizando modos de vida tradicionais. Neste artigo, parte-se da percepção de mulheres de Campos Lindos/TO, sobre as consequências trazidas às suas vidas e saúde por empresas produtoras de soja. Em seguida, questiona-se a constituição do Cerrado como zona de sacrifício do desenvolvimento brasileiro, ao concentrar terras para a produção de 75% de quatro commodities agrícolas, desmatar mais de 50% da vegetação nativa, exaurir aquíferos e levar rios à morte, contaminar o ambiente com 73,5% dos agrotóxicos consumidos no País, trazendo implicações para o processo saúde-doença (como intoxicações agudas, malformações, cânceres, desnutrição, adoecimento mental) e para outros biomas do Brasil e países da América do Sul. Conclui-se perscrutando alternativas na perspectiva dos comuns, do decrescimento, dos direitos da natureza e do bem viver, instigando reflexões da saúde coletiva e da agroecologia sobre a contribuição dos saberes e fazeres tradicionais à saúde e à emancipação humana.

ABSTRACT The immense socio-biodiversity of the Brazilian Cerrado can be understood from the ways of life built by the wide range of traditional peoples and communities in their relations with the biome, of which they are guardians. In the last decades, development projects have promoted an accelerated advance in agribusiness, expropriating land, privatizing water, contaminating the environment and threatening or preventing traditional ways of life. In this essay, we start from the perception of women from Campos Lindos/TO, about the consequences brought to their lives and health by companies producing soybeans. Then we questioned the constitution of the Cerrado as a sacrifice zone for Brazilian development, by concentrating land for the production of 75% of four agricultural commodities, deforesting more than 50% of native vegetation, depleting aquifers and causing rivers to die, contaminating the environment with 73.5% of pesticides consumed in Brazil, with implications for the health-disease process (such as acute intoxications, malformations, cancers, malnutrition, mental illness) and for other biomes in Brazil and South American countries. We conclude by examining alternatives in the perspective of the common, of degrowth, of the rights of nature and of good living, instigating reflections from the Collective Health and Agroecology on the contribution of traditional knowledge and practices to health and human emancipation.

Mudanças de paradigmas realizados por mulheres negras na enfermagem / Paradigm changes performed by black women in nursing / Cambios de paradigma realizados por mujeres negras en enfermería

Falar da enfermagem é entender que o significado de cuidar é tão antigo quanto a existência humana e esse conceito de existir leva a uma reflexão sobre o "ser" negro em uma sociedade. Para tanto, no tange a sua luta por liberdade, na árdua trajetória por mudanças de paradigmas, o contexto histórico mundial traz a luta negra contra a escravidão, cujo o negro escravizado era tido como objeto, emergindo em um cenário de exclusão social, discriminação e intolerância

Phylogenetic relationships of the supercontig of sodium channel subunit I (NaV) in 17 species of Anopheles (Diptera: Culicidae)

ABSTRACT Background: Malaria is a global health problem and is transmitted by the Anopheles species. Due to the epidemiological importance of the genus, studies on biological, phylogenetic, and evolutionary aspects have contributed to the understanding of adaptation, vector capacity, and resistance to insecticides. The latter may result from different causes such as mutations in the gene that encodes the sodium channel (NaV). Methods: In this study, the NaV subunit I scaffold of 17 anopheline species was used to infer phylogenetic relationships of the genus Anopheles using Bayesian inference. The evolutionary phylogenetic tree of the NaV gene was aligned in the AliView program and analyzed utilizing Bayesian inference, using the software MrBayes. Results: The anophelines were grouped into five well-supported clusters: 1 - Anopheles darlingi and Anopheles albimanus; 2 - Anopheles sinensis and Anopheles atroparvus; 3 - Anopheles dirus; 4 - Anopheles minimus, Anopheles culicifacies, Anopheles funestus, Anopheles maculatus, and Anopheles stephensi; and 5 - Anopheles christyi, Anopheles epiroticus, Anopheles merus, Anopheles melas, Anopheles gambiae, Anopheles coluzzii, and Anopheles arabiensis. Conclusions: The topology confirms the phylogenetic relationships proposed in studies based on the genome of some anophelines and reflects the current taxonomy of the genus, which suggests that NaV undergoes selection pressure during the evolution of the species. These data are useful tools for inferring their ability to resist insecticides and also help in better understanding the evolutionary processes of the genus Anopheles.

Physical Mapping of the Anopheles (Nyssorhynchus) darlingi Genomic Scaffolds.

The genome assembly of Anopheles darlingi consists of 2221 scaffolds (N50 = 115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of the smallest genomes among Anopheles species. Anopheles darlingi genomic DNA fragments of ~37 Kb were cloned, end-sequenced, and used as probes for fluorescence in situ hybridization (FISH) with salivary gland polytene chromosomes. In total, we mapped nine DNA probes to scaffolds and autosomal arms. Comparative analysis of the An. darlingi scaffolds with homologous sequences of the Anopheles albimanus and Anopheles gambiae genomes identified chromosomal rearrangements among these species. Our results confirmed that physical mapping is a useful tool for anchoring genome assemblies to mosquito chromosomes.

Human acute Chagas disease: changes in factor VII, activated protein C and hepatic enzymes from patients of oral outbreaks in Pará State (Brazilian Amazon).

Oral transmission of Chagas disease has been increasing in Latin American countries. The present study aimed to investigate changes in hepatic function, coagulation factor levels and parasite load in human acute Chagas disease (ACD) secondary to oral Trypanosoma cruzi transmission. Clinical and epidemiological findings of 102 infected individuals attended in the State of Pará from October 2013 to February 2016 were included. The most common symptoms were fever (98%), asthenia (83.3%), face and limb edema (80.4%), headache (74.5%) and myalgia (72.5%). The hepatic enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of 30 ACD patients were higher compared with controls, and this increase was independent of the treatment with benznidazole. Moreover, ACD individuals had higher plasma levels of activated protein C and lower levels of factor VII of the coagulation cascade. Patients with the highest parasite load had also the most increased transaminase levels. Also, ALT and AST were associated moderately (r = 0.429) and strongly (r = 0.595) with parasite load respectively. In conclusion, the present study raises the possibility that a disturbance in coagulation and hepatic function may be linked to human ACD.

Função sexual e incontinência urinária por esforço em mulheres submetidas à histerectomia total com ooforectomia bilateral / Función sexual e incontinencia urinaria de esfuerzo en mujeres sometidas a histerectomía total con ooforectomía bilateral / Sexual function and stress urinary incontinence in women submitted to total hysterectomy with bilateral oophorectomy

RESUMO O objetivo deste artigo é avaliar o índice de função sexual de mulheres submetidas à histerectomia total com ooforectomia bilateral (HT-OB), a prevalência de incontinência urinária por esforço (IUE) e sua associação com a realização desse procedimento cirúrgico em um hospital de referência em Belém (PA). Foram incluídas 162 mulheres, com vida sexual ativa, alocadas em dois grupos: aquelas que realizaram HT-OB em período superior a 12 meses (n=68), e aquelas que não realizaram (n=94). Utilizou-se o questionário female sexual function index (FSFI) para avaliação da função sexual, e um questionário desenvolvido pelos pesquisadores para coletar dados sociais, econômicos e clínicos, incluindo informações quanto à presença de IUE. O valor de significância foi definido como p<0,05. Houve diferença significativa no índice de função sexual entre o grupo HT-OB e o grupo-controle, com escore geral do FSFI de 23,56 e 28,68, respectivamente (p=0,0001). Os domínios desejo, excitação, lubrificação (p<0,0001), orgasmo (p=0,04), satisfação (p=0,0006) e dor (p=0,015) apresentaram escores inferiores em mulheres histerectomizadas. A prevalência de sintomas de IUE no grupo HT-OB foi de 35,3%, sendo observada associação significativa entre a presença desses sintomas e a realização da histerectomia (p=0,02). Mulheres que realizam HT-OB têm maior risco de disfunção sexual, e este procedimento cirúrgico é associado ao desenvolvimento de IUE.

RESUMEN El presente artículo tuvo el objetivo de evaluar el índice de función sexual de mujeres sometidas a histerectomía total con ooforectomía bilateral (HT-OB), la prevalencia de incontinencia urinaria de esfuerzo (IUE) y su asociación con la realización de este procedimiento quirúrgico en un hospital de referencia en Belém (Brasil). Se incluyeron a 162 mujeres con vida sexual activa, que fueron divididas en dos grupos: las que se sometieron a HT-OB durante el período superior a 12 meses (n=68) y las que no lo había hecho (n=94). Se les aplicaron el cuestionario female sexual function index (FSFI), para evaluar la función sexual, y un cuestionario desarrollado por investigadores para recopilar datos sociales, económicos y clínicos, con informaciones en cuanto a la presencia de IUE. El valor de significación fue de p<0,05. Hubo una diferencia significativa en el índice de función sexual entre el grupo HT-OB y el grupo control, con un puntaje general de FSFI de 23,56 y 28,68, respectivamente (p=0,0001). Los dominios deseo, excitación, lubricación (p<0,0001), orgasmo (p=0,04), satisfacción (p=0,0006) y dolor (p=0,015) tuvieron puntajes más bajos en mujeres histerectomizadas. La prevalencia de síntomas de IUE fue del 35,3% en el grupo HT-OB, además se observó una asociación significativa entre la presencia de estos síntomas y la realización de la histerectomía (p=0,02). Las mujeres que se someten a HT-OB tienen un mayor riesgo de disfunción sexual, y este procedimiento quirúrgico está asociado al desarrollo de IUE.

ABSTRACT The objective of this article was to evaluate the sexual function index of women submitted to total hysterectomy with bilateral oophorectomy (THBO), the prevalence of urinary incontinence (SUI) and its association with the performance of this surgical procedure in a reference hospital in Belém, Pará. One hundred sixty-two women with active sexual life were included in two groups: those who have undergone THBO for more than 12 months (n=68), and those who have not (n=94). The Female Sexual Function Index (FSFI) questionnaire was used to evaluate sexual function along with a questionnaire developed by the researchers to collect social, economic and clinical data, including information on the presence of SUI. The significance level was defined as p<0.05. There was a significant difference in the sexual function index between THBO group and the control group, with an FSFI overall score of 23.56 and 28.68, respectively (p=0.0001). Desire, arousal, lubrication (p<0.0001), orgasm (p=0.04), satisfaction (p=0.0006) and pain (p=0.015) domains had lower scores in hysterectomized women. The prevalence of SUI symptoms in THBO group was 35.3%, and a significant association was observed between the presence of SUI and hysterectomy (p=0.02). Women who undergo THBO have a higher risk of sexual dysfunction, and this surgical procedure is associated with the development of SUI.

Development and characterization of a novel l-asparaginase/MWCNT nanobioconjugate.

The enzyme l-asparaginase (ASNase) presents effective antineoplastic properties used for acute lymphoblastic leukemia treatment besides their potential use in the food sector to decrease the acrylamide formation. Considering their applications, the improvement of this enzyme's properties by efficient immobilization techniques is in high demand. Carbon nanotubes are promising enzyme immobilization supports, since these materials have increased surface area and effective capacity for enzyme loading. Accordingly, in this study, multi-walled carbon nanotubes (MWCNTs) were explored as novel supports for ASNase immobilization by a simple adsorption method. The effect of pH and contact time of immobilization, as well as the ASNase to nanoparticles mass ratio, were optimized according to the enzyme immobilization yield and relative recovered activity. The enzyme-MWCNTs bioconjugation was confirmed by thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), Raman and transmission electron microscopy (TEM) studies. MWCNTs have a high ASNase loading capacity, with a maximum immobilization yield of 90%. The adsorbed ASNase retains 90% of the initial enzyme activity at the optimized conditions (pH 8.0, 60 min, and 1.5 × 10-3 g mL-1 of ASNase). According to these results, ASNase immobilized onto MWCNTs can find improved applications in several areas, namely biosensors, medicine and food industry.

Electrical activity of the masseter and supra hyoid muscles during swallowing of patients with multiple sclerosis. / Atividade elétrica dos músculos masseter e supra-hióideo durante a deglutição do paciente com esclerose múltipla.

PURPOSE: characterize the swallowing muscles electromyographic activity in EM. METHODS: we evaluated 60 individuals being 30 with definitive diagnosis in EMG and 30 without neurologic changes. Volunteers provided personal data through interview, and we also obtained data from clinical records on the time of diagnosis, disease clinic form and the EDSS scale score. We then administered the DYMUS questionnaire. All evaluations occurred according to the Swallowing Electromyographic Evaluation Protocol. RESULTS: Saliva swallowing, liquid swallowing with comfortable volume and continuous swallowing showed differences in the groups at the masseter, with higher averages in the comparison group. We verified differences between masseter and supra hyoid in each group, at rest, in saliva swallowing, liquid swallowing with comfortable volume and continued swallowing. In all situations the averages were higher in the supra hyoid, being that the smaller difference occurred at rest and the higher at continuous swallow. We recorded correlations between EDSS and the activity of supra hyoid at saliva swallow, liquid swallow with comfortable volume and swallow continuous. With the DYMUS, we observed correlations with the masseter activity and with the swallowing time. The high punctuation at the DYMUS presented correlations with the increase electrical activity of the masseter. CONCLUSION: patients with more severe conditions show lower supra hyoid electric activity, and the electric activity of the masseter is related to difficulty in swallowing.

OBJETIVO: caracterizar a atividade eletromiográfica dos músculos da deglutição na EM. MÉTODO: foram avaliados 60 indivíduos, sendo 30 com diagnóstico definitivo de EM e 30 sem alterações neurológicas. Foram levantados os dados pessoais dos voluntários e, em seguida, coletados em prontuário tempo de diagnóstico, forma clínica da doença e escore da escala EDSS. Em seguida foi administrado o questionário DYMUS. Todos foram submetidos à realização do Protocolo de Avaliação Eletromiográfica da Deglutição. RESULTADOS: na deglutição de saliva, deglutição de líquido com volume confortável e deglutição contínua, foram verificadas diferenças entre os grupos no músculo masseter, e as médias foram mais elevadas no grupo de comparação. Foram registradas diferenças entre masseter e supra-hióideo no repouso, na deglutição de saliva, na deglutição de líquido com volume confortável e na deglutição contínua, e em todas as situações as médias foram mais elevadas no supra-hióideo, sendo que a menor diferença ocorreu no repouso, e a maior na deglutição contínua. Foram registradas correlações do EDSS com a atividade do supra-hióideo na deglutição de saliva, na deglutição de líquido com volume confortável e na deglutição contínua. Com o DYMUS foram observadas correlações com a atividade do masseter e com o tempo de deglutição. As pontuações elevadas no DYMUS apresentaram correlações com o aumento da atividade elétrica do masseter. CONCLUSÃO: quanto pior o estado clínico do paciente, menor será a atividade elétrica do supra-hióideo, e o aumento da atividade elétrica do masseter está relacionado com a dificuldade de deglutição.

Nanostructured lipid carriers loaded with free phytosterols for food applications.

The objective of this study was to develop nanostructured lipid carriers (NLCs) with free phytosterols (FP) using conventional fats and oils. Lipid matrices (LMs) and NLCs were produced with high oleic sunflower oil, fully hydrogenated canola (CA) and crambe (CR) oils by high-pressure homogenization (HPH). The NLCs were evaluated for hydrodynamic diameter (Z-ave), polydispersity index (PDI), and zeta potential (ZP). The melting behavior and polymorphism were investigated for both, the LMs and NLCs. The NLCs presented particle sizes ranging from 148.23 to 342.10 nm, PDI from 0.275 to 0.481, and ZP between -22.27 and -29.70 mV. The NLCs presented higher thermal resistance than that of the LMs. The use of CA and CR separately in the NLC formulations favored the incorporation of FP. The LMs and NLCs presented crystals in ß-form and in mixtures of ß' and ß forms. The developed NLCs can be used for food enrichment, such as spreads, margarine, and beverages.

Solid lipid nanoparticles as carriers for lipophilic compounds for applications in foods.

Nanotechnology is a new subject of interest in the field of food industry. Therefore, scientific and technological studies have been intensified in the last 10 years because of the promising results associated with the potential application of functional properties in food products, such as physical and chemical stability, protection and controlled release of bioactive compounds, and facilitated solubility of lipophilic compounds. Lipids have been used as raw material for the preparation of nanostructures, mainly owing to the solubilization capacity of lipophilic bioactive compounds, as well as because of the advantage of potentially using natural ingredients for production on an industrial scale. Thus, in this review, we describe the information reported in scientific literature on the chemical, physical, and structural properties of lipids used in the preparation of solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs). We reviewed the production methods; structural lipid components; emulsifying systems; bioactive lipophilic compounds; and the physical, thermal, and oxidative properties of SLN and NLC. In addition, important methods for characterizing these systems with regard to particle size, polydispersity index, zeta potential, morphology, crystallization behavior, and polymorphism are discussed with examples, in order to support studies that consider physical stability during processing and storage. Furthermore, studies on the applications of SLNs and NLCs in foods are only found for model systems, justifying the compilation of a series of studies on the potential applications to encourage future works. In addition, we have described the aspects still under discussion, related to the possible risks and regulatory aspects of nanotechnology in food.

Effects of glow plasma technology on some bioactive compounds of acerola juice.

This work examines the influence of glow plasma technology on vitamins, carotenoids, phenolic content, antioxidant capacity, and color of acerola juice. The effects of nitrogen plasma treatment were observed at different operation conditions: flowrates (from 10 to 20 mL/min) and processing times (5 to 15 min). The study explored the suitability of glow plasma on 40 mL samples, which is considered a large sample of current plasma technology studies. Nitrogen glow plasma increased the content of vitamin A and carotenoids in acerola juice under specific processing conditions. The highest increase of vitamin A and carotenoids was found at gas flow rate of 10 mL/min and 10 min of treatment time. The effect of glow plasma on vitamin C (main vitamin in acerola juice) was not significant, maintaining the retention of vitamin C above 95% under all operating conditions that were studied. The phenolics content reduced by 30% due to reaction with reactive nitrogen species when the juice was treated at the most severe condition (gas flow rate of 20 mL/min and 15 min of treatment time). The antioxidant capacity was little affected by the reactive nitrogen species formed by N2 glow plasma, which remained relatively constant at high plasma flow rate (20 mL/min) but reduced when lower gas flow rates were used (10 mL/min). The typical orange color of the juice improved due to the increase in free carotenoid content. In summary, this study showed that the operating conditions of glow plasma treatment could be adjusted to have a positive influence on vitamin A, carotenoids and color of acerola juice.